• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.138.1-138
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• 2003

Potato mop-top virus(PMTV) was identified from Solanum tuberosum cv. Gawon showing bright chlorotic mottle symptom in Namwon, Korea. Samples were collected green-house in February, 2003. Electron microscopic examination of negatively stained preparation revealed that PMTV were rigid-rod shaped particles about 100-150, 250-300 nm x 18-20 nm in length. In ultrathin sections of leaf tissue from diseased potato plants, cluster of viruses particles were observed in the cytoplasm. TAS-ELISA determined that the virus was serologically related to PMTV. PMTV produced double ring necrotic local lesion in inoculated leaf of Chenopodium amaranticolor in incubated at 15$^{\circ}C$. The PMTV could be detected with RT-PCR using PMTV detectable primer set designed to amplify about 540 bp of the partial CP gene of PMTV

• The Plant Pathology Journal
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• 제25권1호
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• pp.108-111
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• 2009

DNA-based studies, including cloning and genotyping, have become routine in fungal research laboratories. However, preparation of high-quality DNA from fungal tissue requires much time and labor and is often a limiting step for high-throughput experiments. We have developed a quick and safe (QS) DNA extraction method for fungi. Time efficiency and safety in the QS method were achieved by using plate-grown mycelia as the starting material, by eliminating phenol-chloroform extraction procedures, and by deploying a simple electric grinder. This QS method is applicable not only to a broad range of microbial eukaryotes, including true fungi and oomycetes, but also to lichens and plants.

• International Journal of Industrial Entomology and Biomaterials
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• 제17권1호
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• pp.109-113
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• 2008

Realizing the scope of utilizing by-products of silk cocoons by applying appropriate methods is the immediate crave to optimize returns. The nutritive value of pupae suits for human diet, feed for poultry, carps, fish, rabbits, piggery and dogs. The pupal skin, fat, oil, cocoon palade have applications in oleo chemical, soap, glycerin, cosmetic, artificial fibres, membranes and n-triacontanol isolation. The pupal proteins Chitin, Shinki fibroin, Serrapeptidase, glucosamine are latent precursors of post surgical, anti-carcinogenic, anti-inflammative, anti-bacterial, anti-histaminic, gastric, hepatitis, pancreatitis, leukocytopenia, neurological, ophthalmic, blood pressure, cardiac and diabetic medicines and for preparation of vitamins A, E and K. The silk and its proteins sericin and fibroin are potentially used for wound healing, diabetes, impotence, sinusitis, arthritis, edema, cystitis, epididymitis, tissue regeneration, cancer, post-surgical trauma and used as anti-oxidatives, bio-adhesives, ultra violet screens and bio-active textiles. The waste cocoons can be used in making art crafts like garlands, carpets, overcoats, decoratives and greeting cards. The in-depth research towards utility optimization and make aware this reality to sericulturists, reelers, weavers, traders, entrepreneurs, policy makers etc., is the upright want of the today's Sericulture industry.

• Environmental Analysis Health and Toxicology
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• 제11권3_4호
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• pp.45-51
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• 1996

Histopathological changes of gills and caudal fins isolated from fishes, Cyprinus carpio, Carassius auratus, and Hernibarbus labeo, which were killed by oxygen deficiency or toxic chemicals, were studied. The toxic chemicals were HCl, NaOH, chloroform, benzene, heavy metals(Cu, Cr, Zn, Pb, Hg), and o-dichlorobenzene. The exposure level was enough to kill the fishes within 30 minutes. Oxygen deficient water was prepared by aeration of nitrogen gas and the oxygen concentration was less than l ppm. Cryocutting was used for the rapid preparation of tissue slides and the tissues were stained by hematoxylin/eosin. In the fishes killed by hazardous chemicals, congestion and/or hyperplasia of secondary lamella and erosion of fin were found as the major histopathological changes. Whereas, these characteristics were not observed in gills or caudal fins of fishes killed by oxygen deficiency. These different bioindications appeared in the fishes killed by toxic substances or natural causes, can be used for the rapid identification of the causes of fish kills.

• 대한방사선기술학회지:방사선기술과학
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• 제21권2호
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• pp.47-52
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• 1998

Tissue-equivalent $MgB_4O_7$ TL phosphors were prepared and the effects of Lantanides series activators on TLD properties of the phosphors were studied. The glow curve of TLD increased TL intensity and the peak temperature is low that is, the heating rate is $10^{\circ}C/sec$. The activation energy of the main peak estimated by the peak shape method. By these methods, the estimated activation energies were $0.76{\sim}1.55eV$ respectively. The TL phosphors prepared in this work may be utilized to radiation sensor elements because of this high sensitivity to X-ray.

• Macromolecular Research
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• 제15권6호
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• pp.527-532
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• 2007

Polymer hydrogel has attracted considerable interest as a soft material which is finding expanding applications in pharmaceutics and various biomedical fields. In this work, modified PNVP hydrogels were synthesized by crosslinking polymerization of NVP monomer in the presence of PEG macromer with a methoxy end. The effect of the tethered PEG chain on the properties of the hydrogel was investigated in terms of its swelling capacity, compression gel strength, and the morphology of the resulting hydrogels. These PEG-modified PNVP hydrogels possessed good biocompatibility and a decreased protein (fibrinogen) adsorption, thereby indicating their potential as novel drug delivery matrices and scaffold for tissue engineering.

• Journal of Periodontal and Implant Science
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• 제43권6호
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• pp.262-268
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• 2013

The use of dental implants has become a mainstay of rehabilitative and restorative dentistry. With an impressive clinical success rate, there remain a few minor clinical issues with the use of implants such as peri-implant mucositis and peri-implantitis. The use of laser technology with implants has a fascinating breadth of applications, beginning from their precision manufacturing to clinical uses for surgical site preparation, reducing pain and inflammation, and promoting osseointegration and tissue regeneration. This latter aspect is the focus of this review, which outlines various studies of implants and laser therapy in animal models. The use of low level light therapy or photobiomodulation has demonstrated its efficacy in these studies. Besides more research studies to understand its molecular mechanisms, significant efforts are needed to standardize the clinical dosing and delivery protocols for laser therapy to ensure the maximal efficacy and safety of this potent clinical tool for photobiomodulation.

• 한국세라믹학회지
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• 제45권10호
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• pp.631-637
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• 2008

Self-standing mesoporous bioactive glass/poly($\varepsilon$-caprolactone) composite thin films with good molding capability, bioactivity, and biocompatibility in vitro, which may find potential applications in tissue engineering and drug storage, were prepared using a combination of the sol-gel, polymer templating, and water casting method. The thickness of self-standing films was affected by the difference of dielectric constant between distilled water and organic solvent.

• 한국정밀공학회지
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• 제33권1호
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• pp.63-67
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• 2016

We present a method of fabricating poly (lactic-co-glycolic acid) (PLGA) porous microfibers using a pore template. PLGA microfibers were synthesized using a glass capillary tube in a poly-(dimethylsiloxane) (PDMS) microfluidic chip. Gelatin solution was used as a porous template to prepare pores in microfibers. Two phases of PLGA solutions in different solvents-DMSO (dimethyl sulfoxide) and DCM (dichloromethane)-were used to control the porosity and strength of the porous microfibers. The porosity of the PLGA microfibers differed depending on the ratio of flow rates in the two phases. The porous structure was formed in a spiral shape on the microfiber. The porous structure of the microfiber is expected to improve transfer of oxygen and nutrients, which is important for cell viability in tissue engineering.

• 약학회지
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• 제41권5호
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• pp.602-606
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• 1997

Peonjahwan composed of crude herb, and materials and tissue of animals is a considerably bitter and poorly water-soluble oriental medicine for the treatment of hepatitis. Peonja dry elixir and Eudragit-coated peonja dry elixir were prepared using spray-dryer to mask the bitterness and enhance the release of ingredients from peonjahwan. The bitterness of peonja dry elixir and Eudragit-coated peonja dry elixir reduced to 1/2 and 1/4 of that from peonja powder, respectively. Furthermore, the release rate of bound bilirubin from dry elixir was significantly higher than that from peonja powder.