• 제목/요약/키워드: Tissue paper

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Color Image Analysis of Histological tissue Sections (해부병리조직에 대한 칼라 영상분석)

  • Choe, Heung-Guk
    • The Transactions of the Korea Information Processing Society
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    • v.6 no.1
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    • pp.253-260
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    • 1999
  • In this paper, we suggest a new direct method for mage segmentation using texture and color information combined through a multivariate linear discriminant algorithm. The color texture is computed in nin 3${\times}$3 masks obtained from each 3${\times}$3${\times}$3 spatio-spectral neighborhood in the image using the classical haralick and Pressman texture features. Among these 9${\times}$28 texture features the best set was extracted from a training set. The resulting set of 10 features were used to segment an image into four different regions. The resulting segmentation was Compared to classical color and texture segmentation methods using both box classifiers and maximum likelihood classification. It compared favourably on the test image from a Fastred-Lightgreen stained prostatic histological tissue section based on visual inspection. The classification accuracy of 97.5% for the new method obtained on the training data was also among the best of the tested methods. If these results hold for a larger set of images, this method should be a useful tool for segmenting images where both color and texture are relevant for the segmentation process.

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Multiple congenital anomalies in a Korean native calf (Bos taurus coreanae)

  • Islam, Mohammad Rafiqul;Roh, Yoon-Seok;Cho, Ara;Park, Heejin;Heo, Soo Young;Lee, Kichang;Lee, Han Kyung;Lim, Chae Woong;Kim, Bumseok
    • Korean Journal of Veterinary Research
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    • v.51 no.1
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    • pp.63-67
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    • 2011
  • Supernumerary ectopic limb(s) (SEL) is a congenital anomaly defined as the presence of accessory limb(s) attached to various body regions. This paper describes a case of SEL with ectopic lung and ectopia cordis in a newborn calf, based on macroscopic, microscopic and radiographic findings. External features of multiple congenital anomalies included an ectopic lung growing over the middle of the backbone and covered with normal haired skin. Ectopia cordis was found in the abdominal cavity and attached to the liver. Two extra abnormal limbs originated separately from within the ectopic lung. Most of the abdominal organs were exposed to the outside through the opened abdominal cavity. Microscopically the ectopic lung tissue had edema in the connective tissue around the bronchus and artery. Changes in other organs included congestion of the renal medulla, infiltration of inflammatory cells (lymphocytes and eosinophils) around the hepatic portal tract, and edema surrounding blood vessels and neurons in the brain. The rudimentary humerus of the forelimb was attached to the thoracic spine, as viewed radiographically. The hindlimb was consisted of an irregularly shaped femur, short tibia and fibula, two tarsal bones, one metatarsal bone, and three phalanges. This is the first description of congenital anomalies involving the SEL, ectopic lung and ectopia cordis in a calf.

Mineral- and Tissue-Specific Metabolic Changes in Tomato (Lycopersicon esculentum L.) Plants Grown under NPK-Starved Conditions

  • Sung, Jwakyung;Lee, Yejin;Lee, Seulbi;Lim, Jungeun;Lee, Deogbae
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.6
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    • pp.689-698
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    • 2016
  • Specific metabolic network responses to mineral starvation are not well-defined. We examined a detailed broad-scale identification of metabolic responses of tomato leaf and root to N, P or K starvation. Tomato plants were grown hydroponically under optimal (5 mM N, 0.5 mM P, or 5 mM K) and starved (0.5 mM N, 0.05 mM P, or 0.5 mM K) conditions and metabolites were measured by LC-MS and GC-MS. Overall, the levels of metabolites (lipids, nucleotides, peptides and secondary metabolites) presented in this paper largely showed mineral- and tissue-specific responses. Most strikingly, G3P (glycerol-3-P), GPC (glycerol-P-choline) and choline phosphate responded differently to a type of mineral; an increase in N or K starvation and a decrease in P starvation. A dramatic increase in the levels of secondary metabolites, in particular, rutin and chlorogenate in both tomato tissues during N starvation were observed. Based on these data, it is necessary to clearly elucidate an unknown event taking place in a variety of abiotic impacts, and we are now studying to expand our knowledge on metabolic- and proteomic-responses using GS-MS and LC-MS.

Assessing the Potential of Thermal Imaging in Recognition of Breast Cancer

  • Zadeh, Hossein Ghayoumi;Haddadnia, Javad;Ahmadinejad, Nasrin;Baghdadi, Mohammad Reza
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.18
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    • pp.8619-8623
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    • 2016
  • Background: Breast cancer is a common disorder in women, constituting one of the main causes of death all over the world. The purpose of this study was to determine the diagnostic value of the breast tissue diseases by the help of thermography. Materials and Methods: In this paper, we applied non-contact infrared camera, INFREC R500 for evaluating the capabilities of thermography. The study was conducted on 60 patients suspected of breast disease, who were referred to Imam Khomeini Imaging Center. Information obtained from the questionnaires and clinical examinations along with the obtained diagnostic results from ultrasound images, biopsies and thermography, were analyzed. The results indicated that the use of thermography as well as the asymmetry technique is useful in identifying hypoechoic as well as cystic masses. It should be noted that the patient should not suffer from breast discharge. Results: The accuracy of asymmetry technique identification is respectively 91/89% and 92/30%. Also the accuracy of the exact location of identification is on the 61/53% and 75%. The approach also proved effective in identifying heterogeneous lesions, fibroadenomas, and intraductal masses, but not ISO-echoes and calcified masses. Conclusions: According to the results of the investigation, thermography may be useful in the initial screening and supplementation of diagnostic procedures due to its safety (its non-radiation properties), low cost and the good recognition of breast tissue disease.

Synovial sarcoma in the buccal space: a case report (협부 간극에 발생한 활액막 육종: 증례보고)

  • Han, Ji-Hoon;Yoon, Kyu-Ho;Cheong, Jeong-Kwon;Bae, Jung-Ho;Li, Hsueh-Yu;Oh, Young-Il;Shin, Jae-Myung;Baik, Jee-Seon;Park, Kwan-Soo
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.37 no.6
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    • pp.545-549
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    • 2011
  • Synovial sarcoma (SS) is a malignant soft tissue tumor comprising 5-10% of all soft tissue sarcomas. This tumor normally occurs in the paraarticular regions of the extremities but is rare in head and neck sites. SS is sometimes difficult to diagnose because it can mimic benign lesions both clinically and radiologically. This paper presents a rare case of a SS of the buccal space of a 25-year old man. The histology examination and immunohistochemistry of the mass led to a diagnosis of synovial sarcoma. The patient was treated primarily with a surgical resection, followed by radiotherapy and chemotherapy. The follow up examination 17-months after surgery showed no signs of tumor relapse or metastasis.

Identification of Viral RNA by Light Microscopic in situ Hybridization (광학현미경 In Situ Hybridization에 의한 Viral RNA 증명)

  • 최원기;주경웅;김석홍
    • Biomedical Science Letters
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    • v.2 no.2
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    • pp.249-255
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    • 1996
  • In this paper, a in situ hybridization(ISH) has been used to investigate the yield of viral RNA expression from each organ tissues. It is studied to establish a rapidly, specific diagnostic method detecting rabbit haemorrhagic disease virus(RHDV) RNA in 10% formalin-fixed, paraffin-em-bedded tissues of naturally RHDV-infected rabbits using oligonucleotide probe to be made by RHDV total sequences. Biotin was used as the oligonucleotide probe marker. in situ hybridization is detected the virus genome in the cells and tissue as specifically compared with others nucleic acid hybridization method. All ISH procedure of RHDV were completed to Mi-croProbe$^{TM}$ capillary action system within 1-2 hours. In this report, RHDV was distributed widely in the cytoplasm of liver cell and the cortex of kidney but lung tissue and medulla of kidney were showed to positive reaction at locally. Although not entirely free of technical limitations, nucleic acid identification holds advantages over other diagnostic tests, including exquisite sensitivity, specificity, interchangeability and speed. It is expected that, in the immediate future viral nucleic acid detection will be a prominent part of the methods used in histopathology.

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Finite element analysis of tissue differentiation process in fractured bones applied by a composite IM-rod based on a mechano-regulation theory (메카노 규제 이론에 기초한 복합재료 IM-rod가 적용된 골절부의 세포분화과정의 유한요소해석)

  • Son, Dae-Sung;Mehboob, Hassan;Chang, Seung-Hwan
    • Composites Research
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    • v.25 no.5
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    • pp.136-140
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    • 2012
  • This paper describes the bone healing process of fractured long bones such as a tibia applied by composite IM rods using finite element analysis. To simulated tissue differentiation process mechano-regulation theory with a deviatoric strain was implemented and a user's subroutine programmed by a Python code for an iterative calculation was used. To broadly find the appropriate rod modulus for healing bone fractures, composite IM rods were analyzed considering the stacking sequence. To compare mechanical stimulation at fracture gap, two kinds of initial loading conditions were applied. As a result, it was found that the initial loading condition was the most sensitive factor for the healing performance. In case a composite IM rod made of a plain weave carbon fiber/epoxy (WSN3k) had a stacking sequence of $[{\pm}45]_{nT}$, the healing efficiency was the most effective under a initial load of 10%BW.

A Diagnosis Method of Basal Cell Carcinoma by Raman Spectra of Skin Tissue using NMF Algorithm (피부 조직의 라만 스펙트럼에서 NMF 알고리즘을 통한 기저 세포암 진단 방법)

  • Park, Aaron;Baek, Sung-June
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.8
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    • pp.196-202
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    • 2013
  • Basal cell carcinoma (BCC) is the most common skin cancer and its incidence is increasing rapidly. In this paper, we propose a diagnosis method of basal cell carcinoma by Raman spectra of skin tissue using the NMF(non-negative matrix factorization) algorithm. After preprocessing steps, measured Raman spectra is used classification experiments. The weight and the basis can be obtained in a simple matrix operation and a column vector of the matrix decompsed by the NMF. Linear combination of bases and weights, it is possible to approximate the average of Raman spectra. The classification method is to select the class which to minimize the root mean square of the difference of the linear combination and the objective spectrum. According to the experimental results, the proposed method shows the promising results to diagnosis BCC. In addition, it confirmed that the proposed method compared with the previous research result could be effectively applied in the analysis of the Raman spectra.

Acellular Dermal Matrix as a Core Strut for Projection in Nipple Reconstruction: Approaches for Three Different Methods of Breast Reconstruction

  • Park, Gui-Yong;Yoon, Eul-Sik;Cho, Hee-Eun;Lee, Byung-Il;Park, Seung-Ha
    • Archives of Plastic Surgery
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    • v.43 no.5
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    • pp.424-429
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    • 2016
  • Background The objective of this paper was to describe a novel technique for improving the maintenance of nipple projection in primary nipple reconstruction by using acellular dermal matrix as a strut in one of three different configurations, according to the method of prior breast reconstruction. The struts were designed to best fill the different types of dead spaces in nipple reconstruction depending on the breast reconstruction method. Methods A total of 50 primary nipple reconstructions were performed between May 2012 and May 2015. The prior breast reconstruction methods were latissimus dorsi (LD) flap (28 cases), transverse rectus abdominis myocutaneous (TRAM) flap (10 cases), or tissue expander/implant (12 cases). The nipple reconstruction technique involved the use of local flaps, including the C-V flap or star flap. A $1{\times}2-cm$ acellular dermal matrix was placed into the core with O-, I-, and L-shaped struts for prior LD, TRAM, and expander/implant methods, respectively. The projection of the reconstructed nipple was measured at the time of surgery and at 3, 6, and 9 months postoperatively. Results The nine-month average maintenance of nipple projection was $73.0%{\pm}9.67%$ for the LD flap group using an O-strut, $72.0%{\pm}11.53%$ for the TRAM flap group using an I-strut, and $69.0%{\pm}10.82%$ for the tissue expander/implant group using an L-strut. There were no cases of infection, wound dehiscence, or flap necrosis. Conclusions The application of an acellular dermal matrix with a different kind of strut for each of 3 breast reconstruction methods is an effective addition to current techniques for improving the maintenance of long-term projection in primary nipple reconstruction.

EFFECTS OF RARE EARTH NITRATE ON INTERNAL ORGANS AND MINERAL ELEMENTS IN THE SERUM OF BROILER CHICKENS

  • Kehe, X.;Tingxian, X.;Jiangwi, Z.;Shilin, D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.5 no.1
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    • pp.63-67
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    • 1992
  • This paper reports the effects of rare earth nitrate (REN) on the growth of internal organs and mineral elements in serums of broiler chickens. The REN used is mixture of light RE containing mainly La, Ce, Pr and Nd 4 elements. 40 eight-week old AA broiler chickens were divided randomly into four groups that their treatments were respectively 0, 20, 200 and 2000 mg REN/kg feed and the duration of the experiment was 60 days. Responses were measured in terms of internal organic weight and tissue, contents of mineral elements in serums. There were no significant differences between the organ ratios and tissue changes of the 20, 200 mg REN/kg groups and the control group. The organs included the heart, liver, kidneys, lungs, testicles, thyroids, adrenal glands, pancreas, tonsies of caecum, stomachus glandularis, duodenum, ileum and bursa of Fabricius. The liver and thyroid indices of 2000 mg REN/kg group were significantly higher than those of the control group (p < 0.01, p < 0.05) and the adrenal gland index lower (p < 0.05). The pathlolgic changes in the heart, kidney and thyroid were more serious than that of the control group. No significant differences occurred between the contents of K, Na, Mg elements in the serum of all groups, with the exception of the content of Ca, which was higher in the 2000 mg/kg group (p < 0.05). The contents of all trace elements including Mn, Zn and Cu, but except Fe, in the serums of all the treated groups were significantly higher than those of the control group (p < 0.01).