• 제목/요약/키워드: Tissue harvesting

검색결과 68건 처리시간 0.025초

Reconstruction of post-burn anterior neck contractures using a butterfly design free anterolateral thigh perforator flap

  • Lellouch, Alexandre G.;Ng, Zhi Yang;Pozzo, Victor;Suffee, Tabrez;Lantieri, Laurent A.
    • Archives of Plastic Surgery
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    • 제47권2호
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    • pp.194-197
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    • 2020
  • Anterior neck burns represent a major reconstructive challenge due to severe sequalae including restriction in movement and poor aesthetic outcomes. Common treatment options include skin grafting with/without dermal matrices, and loco-regional and distant free flap transfers with/without prior tissue expansion. Such variation in technique is largely influenced by the extent of burn injury requiring resurfacing. In order to optimize like-for-like reconstruction of the anterior neck, use of wide, thin and long flaps such as the anterolateral thigh (ALT) perforator flap have been reported with promising results. Of note, some patients have a tendency towards severe scar contractures, which may be contributed by the greater extent of inflammation during wound healing. We report our experience at 4 years' followup after secondary reconstruction of severe, anterior neck burn contractures in two patients by harvesting the ALT flap with a butterfly design. This technique provides adequate wound resurfacing of the burned neck and surrounding areas, and provides good neck extensibility by addressing both anterior and lateral aspects of the scar defect simultaneously. Such a flap design reduces tension on wound edges and thus, the risk of contracture recurrence in what remains a particularly challenging type of burn reconstruction.

국내 딸기 시들음병 발생실태와 품종별 저항성 분석 (Resistance Analysis of Cultivars and Occurrence Survey of Fusarium Wilt on Strawberry)

  • 남영현;정석기;김남규;유성준;김홍기
    • 식물병연구
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    • 제11권1호
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    • pp.35-38
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    • 2005
  • 2001년에서 2003년까지 국내 딸기주산단지에서 딸기시들음병 발생실태 조사를 실시하였다. 딸기시들음병 발생은 주로 육묘기인 6월부터 8월, 정식기인 9월부터 10월, 수확기인 1월부터 3월까지 발생되었다. 병징은 뿌리가 갈변되어 크라운의 도관을 따라 갈변되었고 짝잎을 형성하였다. 시들음병 발생은 214개 포장 중 37개 포장에서 최대 30%까지 발생되었다. 딸기 시들음병은 Dochiodome, 매향, Redpearl, Samaberry, Akihime 품종 등 조사된 모든 품종에서 발생하였으나, 특히 Samaberry, 매향, Dochiodome 에서 많이 발생하였다. 시들음병 발생토양은 무발병 토양보다 전기전도도, 질소, 인산농도가 높았고, pH는 낮게 나타났다. Dochiodome 와 Samaberry 품종에서 분리된 Fo47, Fo79 균주는 공시된 4품종에 대해 병원성이 강했으며 특히 Dochiodome, Redpearl, 매향 품종은 더 감수성이었다.

수질부 축소술과 전층 피부이식술을 이용한 교차수지 피판술 (Cross Finger Flap with Reduction Pulp Plasty and Full Thickness Skin Graft)

  • 조용현;노시균;이내호;양경무
    • Archives of Plastic Surgery
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    • 제36권5호
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    • pp.674-677
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    • 2009
  • Purpose: Typical cross finger flap is still a good method for reconstruction of fingertip injuries. However, it is necessarily followed by great loss and aesthetically unpreferable result of donor finger. Hereby, we introduce a modification of cross finger flap with reduction pulp plasty and full thickness skin graft, with which we could reduce the defect size of injured fingertip and donor site morbidity at the same time, without any need for harvesting additional skin from other part of hand. Method: This method was performed in the patients with fingertip injuries of complete amputation or in case of loss of fingertip due to necrosis after replantation. Firstly, reduction pulp plasty was performed on the injured finger to reduce the size of defect of fingertip. Additional skin flap was obtained from the pulp plasty. Secondly, cross finger flap was elevated from the adjacent finger to cover the defect on the injured finger. At the same time, defect on the donor finger produced by the flap elevation was covered by full thickness skin graft with the skin obtained from the pulp plasty of injured finger. Results: Flap and graft survived without any necrosis after surgical delay and flap detachment. All of them were healed well and did not present any severe adversary symptoms. Conclusion: Cross finger flap with reduction pulp plasty and full thickness skin graft is an effective method that we can easily apply in reconstruction of fingertip injury. We think that it is more helpful than the usual manner, especially in cases of children with less soft tissue on their fingers for preservation and reduction of the morbidity of donor finger.

가토의 안면-두피 피판 동종이식을 위한 실험용 모델 연구 (An Experimental Study about flap Viability after Harvesting of the Composite Face/Scalp flap for Allotransplantation in Rabbit Model)

  • 서영민;정승문
    • Archives of Reconstructive Microsurgery
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    • 제14권2호
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    • pp.95-104
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    • 2005
  • The aim of this study was to investigate the major vascular system to supply flap, flap survival rate and complications after flap elevation in order to evaluate possibility of the vascularized face/scalp allotransplantation. Forty New Zealand white rabbits were divided into two groups: control group and experimental group. Individuals of control group had a face/scalp composite unit which was composed of skin, subcutaneous tissue and platysma muscle, supplying by bilateral facial artery, temporal artery and auricular artery and draining by external jugular vein. After a flap was elevated, bilateral facial artery, temporal artery and auricular artery were ligated. On the other hand, those of experimental group had the same composite unit as control group with bilateral facial artery, temporal artery and auricular artery being not ligated. We had measured survival area of flaps of the sixteen individuals survived for four weeks in the control group and fourteen in the experimental group by Grid method. The mean survival durations of the flap were 3.7days in the control group, 20.0days in the experimental group. The significant differences in the mean survival durations and survival rate at the 28days were found between the control and experimental group (p<0.05). Mean values about the survival area's fractions of all were $1.3{\pm}4.%$ in the control group and $63.1{\pm}4.8%$ in the experimental group. Those of experimental group was significantly higher than control group statistically (p<0.05). The composite face/scalp flap which we have elevated, supplied by bilateral facial artery, temporal artery, auricular artery and drained by external jugular vein has flap viability enough to be transplanted after its elevation.

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Investigation of the dorsolateral branch of the posterior intercostal artery for use as the pedicle of a free flap: A cadaveric study and case series

  • Nam, Su Bong;Seo, Jung Yeol;Park, Tae Seo;Sung, Ji Yoon;Kim, Joo Hyoung;Lee, Jae Woo;Kim, Min Wook;Oh, Heung Chan
    • Archives of Plastic Surgery
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    • 제46권1호
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    • pp.39-45
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    • 2019
  • Background The dorsolateral branch of the posterior intercostal artery (DLBPI) can be easily found while harvesting a latissimus dorsi (LD) musculocutaneous flap for breast reconstruction. However, it remains unknown whether this branch can be used for a free flap and whether this branch alone can provide perfusion to the skin. We examined whether the DLBPI could be reliably found and whether it could provide sufficient perfusion. Methods We dissected 10 fresh cadavers and counted DLBPIs with a diameter larger than 2 mm. For each DLBPI, the following parameters were measured: distance from the lateral margin of the LD muscle, level of the intercostal space, distance from the spinal process, and distance from the inferior angle of the scapula. Results The DLBPI was easily found in all cadavers and was reliably located in the specified area. The average number of DLBPIs was 1.65. They were located between the seventh and eleventh intercostal spaces. The average length of the DLBPI between the intercostal space and the LD muscle was 4.82 cm. To assess the perfusion of the DLBPIs, a lead oxide mixture was injected through the branch and observed using X-rays, and it showed good perfusion. Conclusions The DLBPI can be used as a pedicle in free flaps for small defects. DLBPI flaps have some limitations, such as a short pedicle. However, an advantage of this branch is that it can be reliably located through simple dissection. For women, it has the advantage of concealing the donor scar underneath the bra band.

No-fat diet for treatment of donor site chyle leakage in vascularized supraclavicular lymph node transfer

  • Seong, Ik Hyun;Park, Jin-Woo;Woo, Kyong-Je
    • 대한두개안면성형외과학회지
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    • 제21권6호
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    • pp.376-379
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    • 2020
  • Supraclavicular lymph node (SCLN) flap is a common donor site for vascularized lymph node transfer for the treatment of lymphedema. Chyle leakage is a rare but serious complication after harvesting SCLN flap in the neck. We report a case of chyle leakage at the SCLN donor site and its successful management. A 52-year-old woman underwent SCLN transfer for treatment of lower extremity lymphedema. After starting a regular diet and wheelchair ambulation on the 3rd postoperative day, the amount of drainage at the donor site increased (8-62 mL/day) with the color becoming milky, which suggested a chyle leak. Despite starting a low-fat diet on the 4th postoperative day, the chyle leakage persisted (70 mL/day). The patient was started on fat-free diet on the 5th postoperative day. The amount of drainage started to decrease and the drain color became more clear within 24 hours. The drainage amount remained less than 10 mL/day from the 8th postoperative day, and we removed the drain on the 12th postoperative day. There was no seroma or other wound complications at follow-up 4 weeks after the operation. The current case demonstrates that a fat-free diet can be a first-line treatment for low output chyle leakage after a SCLN flap.

저면관수 시스템에서 배양액 농도와 Arbuscular 균근균 처리가 분식 미니 장미의 생육 및 개화에 미치는 영향 (Effects of Nutrient Solution Strength and Arbuscular Mycorrhizal Fungi on Growth and Flowering of Potted Miniature Rose in Ebb and Flow System)

  • 이범선;이인호;지성희;손보균;조자용;강종구
    • 생물환경조절학회지
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    • 제13권2호
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    • pp.112-119
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    • 2004
  • 분화장미의 생장에 미치는 배양액 농도와 AMF(Arbuscular Mycorrhizal Fungi) 접종 및 접종시기의 영향을 구명하기 위하여 수행하였다. 연구목적을 달성하기 위하여 저면관수(ebb and flow) 시스템에서 일본 원예시험장 배양액을 6농도(0.125, 0.25, 0.5, 1, 2, 4배)로 처리하고, AMF를 무접종, 삽목 시 접종 및 정식 시 접종 처리하여 식물을 재배하였다. 배양액 농도가 높아짐에 따라 배지 침출액의 EC가 높아졌으며, AMF무접종 처리구와 삽목 시 AMF접종처리구의 배지 침출액 EC변화는 유사하였으나 정식 시 AMF접종처리구의 EC는 상대적으로 낮았다. 배지 침출액의 pH변화는 AMF무접종처리구와 삽목 시 AMF접종처리구가 서로 비슷한 변화를 나타내었으나 정식 시 AMF접종처리구의 경우 pH변화가 크지 않고, 배앙액농도가 높을수록 낮아졌다. 배양액의 농도가 높을수록 초장, 건물중과 엽면적이 증가하는 결과를 보였으며, AMF접종처리에 의해 영양생장 및 생식생장(개화단축 및 개화수)의 증가를 보였으며, 특히 삽목 시 AMF접종처리보다 정식 시 AMF접종처리가 우수하였다. 엽록소 함량에 있어서도 정식 시 AMF접종처리구에서 증가하는 경향을 나타내었다. 배양액농도의 증가에 의해 N, P, K, Na 및 Mn의 엽중함량이 증가되었으며, AMF접종에 의해서도 증가되었으며, 정식 시 AMF접종처리구가 가장 우수하였다.

'유명' 복숭아의 과실 바람들이 증상 (Characteristics of Fruit Flesh Pithiness Symptoms in 'Yumyeong' Peach [Prunus persica (L.) Batsc])

  • 조명동;김용구;박희승
    • 원예과학기술지
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    • 제18권3호
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    • pp.360-367
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    • 2000
  • 유명복숭아는 보구력이 매우 강한 독특한 불용질성 품종으로서, 수확기간이 긴 장점이 있으나 너무 늦게 수확하는 경우 수상에서 과실 바람들이 증상이나 내부갈변 현상이 나타나는 경우가 있다. 바람들이 증상은 늦게 수확할수록 큰 과실에서 많이 발생하며 당과 말산 함량은 정상과와 비교하여 차이가 없으나 구연산의 함량이 크게 낮아 산도가 떨어졌다. 바람들이 증상과의 과피에서는 질소, 인산, 칼륨의 함량이 높았으며 과육에서는 질소, 인산, 마그네슘의 함량이 높았으나 칼슘의 경우 과피와 과육 모두에서 낮게 나타났다. 바람들이 발생은 수확전 2개월 간의 강수량이 적고 최고온도가 높은 해에 많이 발생하여 과실비대기의 고온건조가 한 원인이 되는 것으로 생각되었다. 형태학적인 측면에서 바람들이 발생부위의 세포들은 정상부위의 세포들보다 작은 크기의 세포들로 이루어져 있었으며, 내부갈변과에서 액포막이 파괴되고 죽은 세포가 많은 것에 비하여 바람들이과는 액포막의 파괴없이 액포가 수축된 상태로 나타났다. 바람들이 발생조직 주변의 유관속 조직에서 틸로시스가 발견되어 이러한 틸로시스가 일부 과육조직의 발달을 방해하여 바람들이 발생에 관여하는 것으로 추정되었다. 한편, '판타지아'와 '월미' 등의 다른 복숭아에서도 틸로시스가 나타났으며 비정상적으로 작은 세포들의 모임이 과육에서 발견되어 과육 전체 세포들이 균일하게 생장하고 있지 못함을 보여주었다. 이는 복숭아 여러 품종에서 바람들이와 유사한 증상이 나타날 수 있는 가능성을 암시하나 많은 복숭아가 연화기간이 짧아 바람들이 증상이 발견되기 어려운 것으로 추정되었다.

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일광에 의해 야기되는 '부유' 단감의 저장 중 과피흑변 (Sunlight Causes Skin Blackening in 'Fuyu' Persimmon Fruits during Storage)

  • 최성진
    • 원예과학기술지
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    • 제33권1호
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    • pp.55-60
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    • 2015
  • 과피흑변은 '부유' 단감(Diospyros kaki, 'Fuyu') 과실의 저장 중에 발생하는 주요 생리적 장해의 하나이다. 부유 과실의 과피에서 페놀화합물의 함량, PPO와 POD의 활성, 지질 포화도를 분석하였다. 페놀화합물은 일광의 방향에 따라 과피에 불균등하게 분포하였는데, 일광 노출면에 축적량이 가장 많았고 그 반대면에서는 가장 적었다. 그러나 수관 내부의 음지 가지에서 수확한 과실의 경우 페놀화합물은 과피에 비교적 고르게 분포되어 있었다. PPO와 POD의 활성 또한 과피에서 고르지 않았으나, 이는 과피흑변이 발생한 과실에서만 그러하였으며, 흑변 부위는 PPO 활성은 가장 높고 POD 활성은 가장 낮았다. 수확 시에 일광면에 표식을 한 과실을 저온에 저장하였을 때, 저장 중 과피흑변의 증상인 운형 흑반은 정확히 표식 부위에 발생하였다. 게다가, 일광면은 높은 지질포화도를 보였는데, 이는 일광에 의해 야기된 고온에 대한 적응을 의미한다. 따라서 이러한 결과로 미루어 볼 때, 부유 과실의 과피흑변 장해는 일광에 노출된 과실의 고온 순화 과피 조직에서 나타나는 저온장해 증상의 일종인 것으로 간주할 수 있을 것이다.

무병종서 생산을 위한 감자X바이러스 및 엽권바이러스에 관한 연구 (Studies on the Potato Virus X and Potato Leaf Roll Virus for Disease-free Seed Potato Production)

  • 최정일
    • 한국작물학회지
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    • 제7권1호
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    • pp.31-63
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    • 1969
  • A series of experiment was carried out to study on the production of disease-free seed potatoes at the Alpine Experiment Station from 1960 to 1968, which initiated a study of comparison on degeneration of plain warm region and high altitude products and the effect of latent potato virus X (PVX) and potato leaf roll virus(PLRV) on degeneration. Particular observations were made on some aspect of the nature of potato virus disease and its control such as concentrations of PVX, range of host plants, physical properties such as concentrations of PYX, range of host plants, physical properties and carrying effect of insects, by investigating 9 different areas of the main potato producing regions (Kimhae, Taegu, Choongju, Taejoen, Suwon, Kwangju, Chonju, Cheju and Chinju). Highly purified anti-serum was separated and tested for control of the virus disease and also various method of prevention and control of PLRV were observed, using cultivation of sprouted seed tubers, early harvesting method, and systemic chemicals. The results obtained are summarized as follows; 1. Potato yield in the plain region decreased by 32.8~66.3% in the first year cultivation of seed potatoes from colder region, and the rate of virus infection was 92.9 to 95.4%. 2. Plants of three families including, 20 species were susceptible to the PVX, and among the plants Salvia officinalis of a habits only was the carrier while the symptom of Digitalis purpurea of Screphulariaceae was masked. Necrosis and ring spot was occurred in most pJants of the Solanaceae and ring spot symptom also was observed in Nicotiana tabacum L. var. White Burley and in N. glutinosa. 3. The 8$C_2$ strain of virus had the following physical properties; thermal inactivation point, 68-$72^{\circ}C$ : dilution inactivation point, above 1, 000, 000 dilution: ageing in vitro, 240-360 days: and ageing in dry plant tissue, 30 days. 4. Myzus persicae and Oxya spp. did not transmit the 8$C_2$ strain of potato virus. 5. Virus was purified through the ammonium sulphate isolating method, and higher titer value, 1/2048 was obtained through anti-serum test. 6. Inhibition Chenopodiacae on the virus infection of potato was remarkable, and inhibition of local lesion host also was observed. 7, By earlier planting of sprouted seed tubers, growth period could be prolonged by 10 to 12 days. 8. Earlier harvest decreased much the rate of virus infection of seed potatoes. 9. According to the results of aphid control trial using systemic soil insecticides at Kangnung and Taekwanlyung, PSP 204, Disyston and Thimet was effective to aphid control. In particular, control effect of twice treatments of PSP 204 was great. 10. Treatmental effect of those chemicals lasted about 60-70 days. However, single foliar application of emulsified chemicals was not effective to potato virus control. 11. The effect of PSP 204, Disyston, and Thimet on the control of potato leaf roll virus was great, particularly in the case of two treatments of PSP 204, at Kangnung as well as at Taekwanlyung. Higher negative correlationship between the control effect of potato leaf roll virus and potato yield was observed showing the value r=-0.85 at Kangnung, and r=-0.87 at Taekwanlyung. 12. Differences in the control effects among PSP 204, Disyston, and Thimet was not noticed.

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