• Title/Summary/Keyword: Tip-effect

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Micropropagation through Stem, Node-bud Shoot Tip and Bulblet Scale Culture in Fritillaria thunbergii Miq. (패모의 줄기, 마디, 정단 및 자구인편 배양에 의한 기내 증식)

  • Peak, Kee-Yoeup;Yu, Kwang-Jin;Seong, Nak-Sul;Choi, In-Sick;Cho, Jin-Tae
    • Korean Journal of Medicinal Crop Science
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    • v.2 no.2
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    • pp.154-161
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    • 1994
  • This experiment was carried out to establish micropropagation system in Fritillaria thunbergii Miq. Through the culture of bulblet scales, stems, node-buds and shoot tips with special reference to the effect of physiological age of explant and plant growth regulators on bulblet formation. Number of formed bulblets was significantly increased in node-bud or stem tissue compared to scals segments and on the medium supplemented with kinetin than BA containing medium. Optimum levels of kinetin for bulblet formation from node-bud taken from above 3 cm shoot length and stem segments excised from below 3 cm shoot length were 5.0 mg /L and $1.0{\sim}3.0\;mg$ /L kinetin, respectively. Interesting phenomenon was observed, the direct formation of bulblets from the axilliary bud of cultured explants. Bulblet forming capacity in stem tissue was depended on stem age, young stem had high regeneration ability compared to old stem taken from above 10 cm shoot length. 1.0 mg /L kinetin was optimum concentration for the formation of bulblets from old stem segments. Stem tissue taken from underground growing plant was promoted coampare to shoot tips or bulb scale segments. Optimum concentration of sucrose was $5{\sim}7%$. Summariged above results revealed that effective explant for micropropagation was stem and /or node-bud tissue excised from less than 3 cm plant height compared to those of bulb scale segments which showed high contamination after culture. Maximum multiplication rate of young stem and /or node-bud segment was about 20 times. Kinetin requirement for stimulation of bulblet formation from cultured explant depended on source of explants but favorable levels of kinetin for organogenesis ranged from 1.0 mg /L to 5.0 mg /L.

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Modified S-FPZ Model for a Running Crack in Concrete (콘크리트의 연속적인 균열성장에 대한 수정 특이-파괴진행대 이론)

  • Yon, Jung-Heum
    • Journal of the Korea Concrete Institute
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    • v.15 no.6
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    • pp.802-810
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    • 2003
  • In this paper, the modified singular fracture process zone (S-FPZ) model is proposed to consider variation of a fracture criterion for continuous crack propagation in concrete. The fracture properties of the proposed fracture model are strain energy release rate at a micro-crack tip and crack closure stress (CCS) versus crack opening displacement (COD) relationship in the FPZ. The proposed model can simulate the estimated fracture energy of experimental results. The analysis results of the experimental data shows that specimen geometry and loading condition did not affect the CCS-COD relation. But the strain energy release rate is a function of not only specimen geometry but also crack extension. Until 25 mm crack extension, the strain energy release rate is a constant minimum value, and then it increased linearly to the maximum value. The maximum fracture criterion occurred at the peak load for an large size specimen. The fracture criterion remains the maximum value after the peak load. The variation of the fracture criterion is caused by micro-cracking and micro-crack localizing. The fracture criterion of strain energy release rate can simply be the size effect of concrete fracture, and it can be used to quantify the micro-tracking and micro-crack localizing behaviors of concrete.

Effects of the Angulation of Orthodontic Mini-Implant as an Indirect Anchorage : A Three-Dimensional Finite Element Analysis (교정용 미니임플란트의 식립각도에 따른 간접골성 고정원의 효과에 대한 유한요소 해석)

  • Kim, Min-Ji;Park, Yong-Jin;Park, Sun-Hyung;Chun, Youn-Sic
    • Journal of Dental Rehabilitation and Applied Science
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    • v.27 no.3
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    • pp.293-304
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    • 2011
  • The purpose of this study was to investigate the displacement and pattern of stress distribution on periodontal ligaments of maxillary first and second molar, and on orthodontic mini-implant (OMI) surface, according to three different insertion angles to the bone surface of OMI using Dragon helix appliance, which is a newly introduced scissors-bite correcting appliance. OMI were placed between second premolar and first molar with three different insertion angles (45, 60, 90 degrees). Displacement and maximum stress distribution area (MSDA) were analyzed by finite element analysis. When the insertion angle to the alveolar bone surface was 90 degrees, maxillary first and second molar both exhibited MSDA at the palatal root apex. Maxillary first molar did not show any significant displacement, while the second molar exhibited intrusive and palatal displacement. On the OMI, as the insertion angle decreased, the MSDA shifted towards the tip, and the amount of displacement had increased. When the OMI was inserted at a 90 degree angle, anchor loss was minimized and scissors-bite correcting effect was maximized.

PEMOCVD of Ti(C,N) Thin Films on D2 Steel and Si(100) Substrates at Low Growth Temperatures

  • Kim, Myung-Chan;Heo, Cheol-Ho;Boo, Jin-Hyo;Cho,Yong-Ki;Han, Jeon-Geon
    • Proceedings of the Korean Vacuum Society Conference
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    • 1999.07a
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    • pp.211-211
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    • 1999
  • Titanium nitride (TiN) thin films have useful properties including high hardness, good electrical conductivity, high melting point, and chemical inertness. The applications have included wear-resistant hard coatings on machine tools and bearings, decorative coating making use of the golden color, thermal control coatings for widows, and erosion resistant coatings for spacecraft plasma probes. For all these applications as feature sizes shrink and aspect ratios grow, the issue of good step coverage becomes increasingly important. It is therefore essential to manufacture conformal coatings of TiN. The growth of TiN thin films by chemical vapor deposition (CVD) is of great interest for achieving conformal deposition. The most widely used precursor for TiN is TiCl4 and NH3. However, chlorine impurity in the as-grown films and relatively high deposition temperature (>$600^{\circ}C$) are considered major drawbacks from actual device fabrication. To overcome these problems, recently, MOCVD processes including plasma assisted have been suggested. In this study, therefore, we have doposited Ti(C, N) thin films on Si(100) and D2 steel substrates in the temperature range of 150-30$0^{\circ}C$ using tetrakis diethylamido titanium (TDEAT) and titanium isopropoxide (TIP) by pulsed DC plamsa enhanced metal-organic chemical vapor deposition (PEMOCVD) method. Polycrystalline Ti(C, N) thin films were successfully grown on either D2 steel or Si(100) surfaces at temperature as low as 15$0^{\circ}C$. Compositions of the as-grown films were determined with XPS and RBS. From XPS analysis, thin films of Ti(C, N) with low oxygen concentration were obtained. RBS data were also confirmed the changes of stoichiometry and microhardness of our films. Radical formation and ionization behaviors in plasma are analyzed by optical emission spectroscopy (OES) at various pulsed bias and gases conditions. H2 and He+H2 gases are used as carrier gases to compare plasma parameter and the effect of N2 and NH3 gases as reactive gas is also evaluated in reduction of C content of the films. In this study, we fond that He and H2 mixture gas is very effective in enhancing ionization of radicals, especially N resulting is high hardness. The higher hardness of film is obtained to be ca. 1700 HK 0.01 but it depends on gas species and bias voltage. The proper process is evident for H and N2 gas atmosphere and bias voltage of 600V. However, NH3 gas highly reduces formation of CN radical, thereby decreasing C content of Ti(C, N) thin films in a great deal. Compared to PVD TiN films, the Ti(C, N) film grown by PEMOCVD has very good conformability; the step coverage exceeds 85% with an aspect ratio of more than 3.

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A study on the effect of the locations of pile tips on the behaviour of piles to adjacent tunnelling (말뚝선단의 위치가 터널근접 시공에 의한 말뚝의 거동에 미치는 영향에 대한 연구)

  • Lee, Cheol-Ju;Jeon, Young Jin
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.17 no.2
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    • pp.91-105
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    • 2015
  • In the current work, a series of three-dimensional (3D) finite element analyses have been performed to study the effects of the locations of pile tips on the behaviour of single piles to adjacent tunnelling. In the numerical modelling, several key issues, such as tunnelling-induced pile head settlements, axial pile forces, interface shear stresses and apparent factors of safety have been studied. When the pile tips are inside the influence zone which considers the relative pile tip location with respect to the tunnel position, tunnelling-induced pile head settlements are larger than those computed from the greenfield condition. However, when the pile tips were outside the influence zone, an opposite trend was observed. When the pile tips were inside the influence zone, tunnelling-induced tensile pile forces developed; however, when the pile tips were outside the influence zone, tunnelling-induced compressive pile forces were mobilised, associated with larger settlements of the surrounding soil than the pile settlements. It has been shown that the increases in the tunnelling-induced pile head settlements have resulted in reductions of the apparent factor of safety by about 50% when the pile tips are inside the influence zone, therefore severly affecting the serviceability of piles. The pile behaviour, when considering the location of pile tips with regards to the influence zone, has been analysed in great detail by taking the tunnelling-induced pile head settlements, axial pile force and apparent factor of safety into account.

Development of Uneven Excavation Method for Reinforcement of Ground Slope (사면보강을 위한 요철형 암반굴착 공법개발)

  • Kim, Hyun-Gi
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.10
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    • pp.259-266
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    • 2020
  • In this study, required drill bits and excavation methods were developed for an uneven drilling method that can solve the problem of performance degradation of rock bolts. The developed drill bit's excavation performance was verified using rock with a strength of 100 MPa or more. In addition, for the relative evaluation of the uneven excavation method, experimental specimens were prepared for models with and without irregularities, and tests were performed. As a result of the experiment, the model with unevenness exhibited an average critical draw resistance of 801.6 kN, which is about 1.7 times the value of 468.7 kN for the model without unevenness, thus confirming the effect sufficiently. Therefore, it is expected that the resistance performance will significantly increase despite an increase in the uneven hole diameter of 20 mm. In the future, the results of this study could be used as basic data when performing other studies using numerical analysis models and performance verification through experiments to obtain an optimized rock forming method.

The Effect of Various Culture Media on Histological Anatomy and Saikosaponin Content in Hairy Root Culture of Buplurum falcatum (시호 모상근 배양에서 배지성분에 따른 사이코사포닌 함량과 조직학적 형태의 차이)

  • Ahn, Jun-Cheul;Jeong, Young-Jae;Lee, Kyu-Bae;Kim, Ock-Tae;Hwang, Baik
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.4
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    • pp.251-256
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    • 1999
  • Growth rate and saikosaponin content of Buplurum falcatum hairy root on MS and RCM basal media were measured. And using hairy root cultured in those media, it was investigated whether some correlation between differences in growth rate and saikosaponin content and histiological difference was or not. Results obtained showed that among media tested, MS and 3RCM medium which showed the faster growth in fresh weight and dry weight, respectively. And saikosaponin content of hairy root cultured in 3RCM was $2.2{\sim}5.5$ times higher than that of 1 year cultivated roots, whereas that in MS was extremely low. On the other hand, histological differences of root tips and about 1 cm region from root tip cultured in both MS and 3RCM were not prominent, however, in mature region, a lot of cells only in 3RCM contained densely stained vacuoles or organelles which probably contain tannin, suggesting that development of those has a correlation with biosynthesis of secondary metabolites containing saikosaponin.

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A Simulation of Wave Induced Current Around the Jeju New Harbor (제주외항 건설에 따른 주변 해역의 해빈류 변화 예측)

  • Kwak, Moon-Su;Pyun, Chong-Kun
    • Journal of the Korean Society of Hazard Mitigation
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    • v.4 no.3 s.14
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    • pp.1-8
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    • 2004
  • In this study, the change of the current in the coastal zone before and after the construction of Jeju new harbor was predicted by using the numerical model, which uses Hardy-Cross method. The numerical model was carried out for the present state, before the construction, and the state after the construction, and for the wave direction the NNW direction for winter and NE direction for summer were tested so that the seasonal change may be considered. The computation result shows that a large amount of the wave induced current was occurred when there were high waves coming in from NNW direction before and after the construction. Also, before the construction a longshore current occurred moving from the west to the east at the new harbor construction site so that it formed a rip current in the Hwabuk-dong front sea. And also, after the construction, the tip current produced changed into nearshore circulating current and a small circulating current appeared at the harbor entrance. On the other hand, at Samyang 4each, which is 3.0km away from the new harbor in the NE direction, shows that there was a longshore current occurred from the west to the east, which is in the opposite direction the new harbor, and the effect on the new harbor by sediment transport at Samyang beach is thought to be very small.

Mulberry Yields under Several Planting Densities on Hilly Land (경사지 뽕밭에서 식재밀도에 따른 수량의 경년적 변화에 관한 연구)

  • 이종한;김동일
    • Journal of Sericultural and Entomological Science
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    • v.29 no.1
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    • pp.7-11
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    • 1987
  • The influence of planting density, on the mulberry yield, had been studied for 5 years period since 1982. For this purpose, a hilly land (15$^{\circ}$) was selected and 5 planting density level (926, 1,667, 2,780 and 3,444 tree/10a) were used. Following results were obtained. 1. Vinyl mulching promoted 2 days earlier of green tip sprouting stage and 3 days earlier of shallow-bill stage, compared with the non-mulching. 2. It was shown that increase of the length of the branch with vinyl mulching and decrease of the mulching effect with the higher density of plants from, 2,780 tree/10a. 3. When the planting density was higher, length and width of leaf was decreased sharply, and chlorosis of the lower part of the branch was occured heavily. 4. With the higher planting density, number of branches per tree and average length of the branch was decreased. Number of shorter branch-normal branch ratio were increased. However, number of branches and total length of branch per area were shown opposite. 5. Yield of mulberry was increased for the peroid of 3 years after planting with the all the levels of planting density after that, yield increase was dully in 926 tree plot, and more dully in 1,677 trees plot, while decrease of the yield were showen with density of 2,780 and 3,444 trees plots. 6. The highest average yield for the 5 years were obtained from 2,780 trees plot, 46.6% higher than in 926 trees plot, followed 40% higher in 3,444 trees plot, and 29.9% higher in 1,667 trees plot. 7. Soil pH, organic matter, Ca and Mg decreased with cultivation, especially P2O5, pH, Ca and Mg in the density of more than 926 trees plot.

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A STUDY ON CLASS II COMPOSITE RESIN CAVITY USING FINITE ELEMENT STRESS ANALYSIS (유한요소법을 이용한 2급 복합레진 와동의 비교 연구)

  • Rim, Young-Il;Yo, In-Ho;Um, Chung-Moon
    • Restorative Dentistry and Endodontics
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    • v.22 no.1
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    • pp.428-446
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    • 1997
  • Restorative procedures can lead to weakening tooth due to reduction and alteration of tooth structure. It is essential to prevent fractures to conserve tooth. The resistance to fracture of the restored tooth may be influenced by many factors, among these are the cavity dimension and the physical properties of the restorative material. The placement of direct composite resin restorations has generally been found to have a strengthening effect on the prepared teeth. It is the purpose of this investigation to study the relationship between the cavity isthmus and the fracture resistance of a tooth in composite resin restorations. In this study, MO cavity was prepared on maxillary first premolar. Three dimensional finite element models were made by serial photographic method and isthmus(1/4, 1/3, 1/2 of intercuspal distance) were varied. Two types of model(B and R model) were developed. B model was assumed perfect bonding between the restoration and cavity wall and R model was left unfilled. A load of 500N was applied vertically at the first node from the lingual slope of the buccal cusp tip. This study analysed the displacement, 1 and 2 direction normal stress and strain with FEM software ABAQUS Version 5.2 and hardware IRIS 4D/310 VGX Work-station. The results were as follows : 1. Displacement of buccal cusp in R model occurred and increased as widening of the cavity, and displacement in B model was little and not influenced by cavity width. 2. There was a significant decrease of stress resulting in increase of fracture resistance in B model when compared with R model. 3. With the increase of the isthmus width, B model showed no change in the stress and strain. In R model, the stress and strain increased both in the area of buccal-pulpal line angle and the buccal side of marginal ridge, therefore the possibility of crack increased. 4. The stress and strain were distributed evenly on the tooth in B model, but in R model, were concentrated on the buccal side of the distal marginal ridge and buccal-pulpal line angle, therefore the possibility of fracture increased.

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