• Fisheries and Aquatic Sciences
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• v.21 no.3
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• pp.5.1-5.8
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• 2018

Stimulator of interferon gene (STING) is induced by various inflammatory agents, such as lipopolysaccharide and microbial pathogens, including virus and bacteria. In this study, we obtained a full-length cDNA of a STING homolog from olive flounder using rapid amplification of cDNA ends PCR technique. The full-length cDNA of Paralichthys olivaceus STING (PoSTING) was 1442 bp in length and contained a 1209-bp open reading frame that translated into 402 amino acids. The theoretical molecular mass of the predicted protein sequence was 45.09 kDa. In the PoSTING protein, three transmembrane domains and the STING superfamily domain were identified as characteristic features. Quantitative real-time PCR revealed that PoSTING expressed in all the tissues analyzed, but showed the highest level in the spleen. Temporal expression analysis examined the significantly upregulated expression of PoSTING mRNA after viral hemorrhagic septicemia virus (VHSV) stimulation. In contrast, no significant changes in the PoSTING expression were detected in Edwardsiella tarda-challenged group compared to the un-injected control. The expression of P. olivaceus type I interferon (PoIFN-I) was also highly upregulated upon VHSV challenge. These results suggest that STING might be involved in the essential immune defense against viral infection together with the activation of IFN-I in olive flounder.

• Journal of the Korean Institute of Telematics and Electronics T
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• v.35T no.1
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• pp.67-74
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• 1998

The primary cause of air pollution by vehicles is imperfect combustion of fuel. One of the most usual causes of this imperfect combustion is the misfire in IC engins. The U.S. EPA(Environment Protection Agency) and the CARB(California air Resources Board) have imposed regulations for the detection of misfiring in automotive engines. The OBD-II regulations require that misfire should be monitored by the engine diagnostic system, and that the goal of OBD-II is to alert the driver to the presence of a malfunction of the emission control system. Several solutions to the misfire detection problem have been proposed for the detection of misfires. However, the performance of these methods in the presence of misfire is not altogether clear. This paper presents a precise method and system for internal combustion engine misfire. Present invention based upon measurements of engine roughness as derived from crankshaft angular velocity measurements with special signal processing method. Crankshaft angular velocity signals are processed by WDPT, so that the more reliable misfire detection than the time domain analysis. Experimental work confirms that it is possible to apply the WDFT for the detection of misfires in no-load idle and road testing.

• Korean Journal of Optics and Photonics
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• v.25 no.4
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• pp.193-199
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• 2014

The net effect of the emitter orientation, Mie scatters, and pillow lenses on the outcoupling efficiency (OCE) of a bottom-emitting OLED having an internal photonic crystal layer was investigated by a combined optical simulation based on the finite-difference time-domain method (FDTD) and the ray-tracing technique. The simulation showed that when the emitter orientation was horizontal with respect to the OLED surface, the OCE could be increased by 54% when a photonic crystal layer was employed, while it could be improved by 86% under optimized conditions of Mie scatters and pillow lenses applied to the glass substrate. The peculiar intensity distribution of the OLED, caused by the periodic lattice structure of the photonic crystal layer, could be ameliorated by inserting Mie scatters into the glass substrate. This study suggests that conventional outcoupling structures combined with control of the emitter orientation could improve the OCE substantially.

• International Journal of Industrial Entomology and Biomaterials
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• v.23 no.2
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• pp.223-229
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• 2011

Cyclophilins are originally identified as cytosolic binding protein of the immunosuppressive drug cyclosporine A. They have an activity of peptidyl prolyl cis/trans-isomerases (PPIase), which may play important roles in protein folding, trafficking, assembly and cell signaling. In this study, we report the cloning and characterization of a Bombyx mori cyclophilin A (bCypA) cDNA. The full-length cDNA of bCypA consist of 947 nucleotides with a polyadenylation signal sequence AATAAA and contain an open reading frame of 498 nucleotides encoding a polypeptide of 166 amino acids. The deduced amino acid sequence of bCypA shares a central peptidyl prolyl cis/trans-isomerase and a cyclosporin-A-binding domain with other cyclophilin sequences. Relative quantification real-time (RT) PCR analysis shows that mRNA transcripts of bCypA are detected in all the investigated tissues and highest expression level in the skin of 3-day-old 5 instar larva. Also, bCypA had PPIase activity on the proline-containing peptides. Accordingly, we suggest that bCypA is a new member of the cyclophilin A (CyPA) family and will be useful for quality control of bioactivity recombinant proteins with proline-containing peptides.

• The Journal of the Acoustical Society of Korea
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• v.24 no.5
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• pp.238-247
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• 2005

In this Paper. we Presents subband design techniques of a compensating filter and adaptive feedback canceller for the digital hearing aid. The sensorineural hearing loss has a hearing threshold that shows a nonlinear characteristic in frequency domain. and its compensation suffers from an echo that produced by an undesired time varying feedback path. Therefore. the digital hearing aid requires the compensator that can adjust gains nonlinearly in frequency bands and eliminate the echo rapidly In the Proposed digital hearing aid. the compensating filter is designed by the adaptive system identification method in subband structure, and the adaptive feedback canceller is designed by the subband affine projection algorithm. The designed compensation filter can control the nonlinear gain in each subband respectively, therefore precise compensation is possible. And the feedback canceller using the subband adaptive filter achieves fast convergence rate. The Performances of the Proposed method are verified by computer simulations as comparing with the behaviors of the previous trials.

• The Journal of Society for e-Business Studies
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• v.18 no.1
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• pp.165-189
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• 2013

Conceptual modeling is a critical activity for developing successful business information systems. The objective of this study is to evaluate the possibility of applying the constant comparison method from the grounded theory to conceptual modeling. To achieve the objective, we trained novice modelers and split them into two groups for evaluation. The experimental results show that applying the constant comparison method could increase acceptability from more experienced conceptual modelers. Moreover, while the control group was experienced difficulties when domain knowledge is unfamiliar, the experimental group could handle difficulties more effectively. In addition, applying the constant comparison method also decreased the time to complete analysis for conceptual modeling.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.5 s.120
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• pp.553-562
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• 2007

In this paper, we designed and implemented the system for telemetering ELF(Extremely Low Frequency) magnetic field intensity. The magnetic field measurement system used a 3-axis magnetic field sensor to measure the magnetic field with isotropy and the equalizer to compensate the frequency characteristic in band. By multiplexing three output signals of the magnetic field sensor in time domain, we got the uniform gain and frequency characteristic among three axes. This system was designed that the magnetic field measurement level range was $0.01{\sim}10.0\;uT$ and the measurement frequency band was $40{\sim}180\;Hz$. The control system would access to the magnetic field measurement system with RF and the maximum access distance was 1.0 km. We confirmed that the measurement level error of the fabricated system was within 5 %. The fabricated system was installed to a golf practice range where a high voltage power transmission line was crossed.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.22 no.8
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• pp.1774-1781
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• 1997

In this paper, we propose a full digital frequency and phase locked loop for CATV and HDTV receivers adopting VSB modulation. The CATV and HDTV receivers proposed by the Grand-Alliance in USA are ultilizing analog signal processing technology for carrier recovery. By the way, it is not a good architecture for the development of single chip ASIC operating in digital domain. To solve this problem while improving the performance, we first down convert the received r.f. signal to a near baseband signal for a low-rate AD converter and then we use digital signal processing techniques. The proposed system has the frequency pull-in range of -200 KHz +2.50 KHz. Moreover, it has the ability of adaptive loop bandwidth control according to the amount of frequency offset to improve the acquisition time while reducing the phase noise.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.4
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• pp.432-440
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• 2019

Global climate change exerts adverse effects on maize production. Among abiotic stresses, drought stress during the tasseling stage (VT) can increase anthesis-silking intervals (ASI) and decrease yield. We performed an evaluation of ASI and yield using a drought-sensitive line (Ki3) and a drought-tolerant line (Ki11) to analyze the correlation with ASI and yield. Moreover, the de novo data of Ki11 were analyzed to find putative novel transcripts related todrought stress in tropical maize. A total of 182 transcripts, with a log2 ratio >1.5, were found by comparing drought conditions to a control. The top 40 transcripts of high expression levels in the de novo analysis were selected and analyzed with PCR. Of the 40 transcripts, six novel transcripts were detected by quantitative real-time PCR (qRT-PCR) using seedling and VT stage samples. Five transcripts (transcripts_1, 12, 34, 35, and 40) were up-regulated in the Ki11 shoot at seedling stage, and transcripts_1, 12, and 40 were up-regulated at the re-watering stage after 12 h of drought stress. The transcripts_32 and 34 were up-regulated at the VT stage. Hence, transcript_34 possibly plays a significant role in drought tolerance during the seedling and VT stages. The transcript_32 was identified as chloramphenicol acetyltransferase (CAT) by Pfam domain analysis. The function of the other transcripts remained unknown. Further characterization of these novel transcripts in genetic regulation will be of great value for the improvement of maize production.

• Journal of Microbiology and Biotechnology
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• v.31 no.11
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• pp.1591-1600
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• 2021

Streptomyces coelicolor is a filamentous soil bacterium producing several kinds of antibiotics. S. coelicolor abs8752 is an abs (antibiotic synthesis deficient)-type mutation at the absR locus; it is characterized by an incapacity to produce any of the four antibiotics synthesized by its parental strain J1501. A chromosomal DNA fragment from S. coelicolor J1501, capable of complementing the abs- phenotype of the abs8752 mutant, was cloned and analyzed. DNA sequencing revealed that two complete ORFs (SCO6992 and SCO6993) were present in opposite directions in the clone. Introduction of SCO6992 in the mutant strain resulted in a remarkable increase in the production of two pigmented antibiotics, actinorhodin and undecylprodigiosin, in S. coelicolor J1501 and abs8752. However, introduction of SCO6993 did not show any significant difference compared to the control, suggesting that SCO6992 is primarily involved in stimulating the biosynthesis of antibiotics in S. coelicolor. In silico analysis of SCO6992 (359 aa, 39.5 kDa) revealed that sequences homologous to SCO6992 were all annotated as hypothetical proteins. Although a metalloprotease domain with a conserved metal-binding motif was found in SCO6992, the recombinant rSCO6992 did not show any protease activity. Instead, it showed very strong β-glucuronidase activity in an API ZYM assay and toward two artificial substrates, p-nitrophenyl-β-D-glucuronide and AS-BI-β-D-glucuronide. The binding between rSCO6992 and Zn²⁺ was confirmed by circular dichroism spectroscopy. We report for the first time that SCO6992 is a novel protein with β-glucuronidase activity, that has a distinct primary structure and physiological role from those of previously reported β-glucuronidases.