• BMB Reports
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• 제45권5호
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• pp.299-304
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• 2012

The ubiquitin-proteasome system is a major proteolytic system for nonlysosomal degradation of cellular proteins. Here, we investigated the response of mouse embryonic stem (ES) cells under proteotoxic stress. Proteasome inhibitors induced expression of heat shock protein 70 (HSP70) in a concentration- and time-dependent manner, and also induced apoptosis of ES cells. Importantly, more apoptotic cells were observed in ES cells compared with other somatic cells. To understand this phenomenon, we further investigated the expression of HSP70 and pluripotent cell markers. HSP70 expression was more significantly increased in somatic cells than in ES cells, and expression levels of pluripotent cell markers such as Oct4 and Nanog were decreased in ES cells. These results suggest that higher sensitivity of ES cells to proteotoxic stress may be related with lower capacity of HSP70 expression and decreased pluripotent cell marker expression, which is essential for the survival of ES cells.

• Structural Engineering and Mechanics
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• 제54권2호
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• pp.319-336
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• 2015

Life cycle performance of corrosion affected RC structures is an important and challenging issue for effective infrastructure management. The accurate condition assessment of corroded RC structures mainly depends on the effective evaluation of deterioration occurring in the structures. Structural performance deterioration caused by reinforcement corrosion is a complex phenomenon which is generally uncertain and non-decreasing. Therefore, a stochastic modelling such as the gamma process can be an effective tool to consider the temporal uncertainty associated with performance deterioration. This paper presents a time-dependent reliability analysis of corrosion affected RC structures associated bond strength degradation. Initially, an analytical model to evaluate cracking in the concrete cover and the associated loss of bond between the corroded steel and the surrounding cracked concrete is developed. The analytical results of cover surface cracking and bond strength deterioration are examined by experimental data available. Then the verified analytical results are used for the stochastic deterioration modelling, presented here as gamma process. The application of the proposed approach is illustrated with a numerical example. The results from the illustrative example show that the proposed approach is capable of assessing performance of the bond strength of concrete structures affected by reinforcement corrosion during their lifecycle.

• Computers and Concrete
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• 제20권5호
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• pp.529-537
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• 2017

Excessively elevated temperature can lead to cracks in prestressed concrete (PC) continuous bridge with box girder on the pier top at cement hydration age. This paper presents a case study for evaluating the behavior of PC box girder during the early hydration age using a two-stage computational model, in the form of computer program ANSYS, namely, 3-D temperature evaluation and determination of mechanical response in PC box girders. A numerical model considering time-dependent wind speed and ambient temperature in ANSYS for tracing the thermal and mechanical response of box girder is developed. The predicted results were compared to show good agreement with the measured data from the PC box girder of the Zhaoshi Bridge in China. Then, based on the validated numerical model three parameters were incorporated to analyze the evolution of the temperature and stress within box girder caused by cement hydration heat. The results of case study indicate that the wind speed can change the degradation history of temperature and stress and reduce peak value of them. The initial casting temperature of concrete is the most significant parameter which controls cracking of PC box girder on pier top at cement hydration age. Increasing the curing temperature is detrimental to prevent cracking.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.222-227
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• 2008

This study is focused on an integrated numerical modeling enabling one to investigate the dynamic behavior and failure of 2-D textile composite and 3-D orthogonal woven composite structures weakened by micro-cracks and subjected to an impact load. The integrated numerical modeling is based on: I) determination of governing equations via a three-level hierarchy: micro-mechanical unit cell analysis, layer-wise analysis accounting for transverse strains and stresses, and structural analysis based on anisotropic plate layers, II) development of an efficient computational approach enabling one to perform transient response analyses of 2-D plain woven and 3-D orthogonal woven composite structures featuring the matrix cracking and exposed to time-dependent loads, III) determination of the structural characteristics of the textile-layered composites and their degraded features under various geometrical yarn shapes, and finally, IV) assessment of the implications of stiffness degradation on dynamic response to impact loads.

• BMB Reports
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• 제47권2호
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• pp.98-103
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• 2014

Orostachys japonicus shows various biological activities. However, the molecular mechanisms remain unknown in LPS-stimulated macrophages. Here, we investigated the anti-oxidizing effect of the dichloromethane (DCM) and hexane fractions from O. japonicus (OJD and OJH) against oxidative stress in RAW 264.7 cells stimulated by LPS. OJD and OJH significantly increased the expression of heme oxygenase-1 (HO-1) in a dose- and time-dependent manner. Additionally, it was found that the expression of HO-1 was stimulated by Nrf2 activated via degradation of Keap1. ERK and p38 inhibitors repressed HO-1 induced by OJD and OJH in LPS-stimulated cells, respectively. In conclusion, these results suggest that OJD and OJH may block oxidative damage stimulated by LPS, via increasing the expression of HO-1 and Nrf2, and MAPK signaling pathway.

• Toxicological Research
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• 제29권4호
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• pp.235-240
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• 2013

Extracellular accumulation of amyloid beta protein ($A{\beta}$) plays a central role in Alzheimer's disease (AD). Some metals, such as copper, lead, and aluminum can affect the $A{\beta}$ accumulation in the brain. However, the effect of mercury on $A{\beta}$ accumulation in the brain is not clear. Thus, this study was proposed to estimate whether mercury concentration affects $A{\beta}$ accumulation in PC12 cells. We treated 10, 100, and 1000 nM $HgCl_2$ (Hg) or $CH_3HgCl_2$ (MeHg) for 48 hr in PC12 cells. After treatment, $A{\beta}_{40}$ in culture medium increased in a dose- and time-dependent manner. Hg and MeHg increased amyloid precursor protein (APP), which is related to $A{\beta}$ production. Neprilysin (NEP) levels in PC12 cells were decreased by Hg and MeHg treatment. These results suggested that Hg induced $A{\beta}$ accumulation through APP overproduction and reduction of NEP.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2005년도 봄학술 발표회 논문집(II)
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• pp.469-472
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• 2005

With all ensuring the fire resistance structure as a method of setting the required cover thickness to fire, the RC is significantly affected from the standpoint of its structural stability that the compressive strength and elastic modulus is reduced by fire. Normally, the degradation of concrete member exposed to fire is largely dependent on the fire scale and fire condition. There is therefore a need to precisely predict the deterioration and fire damage of the exposed member. Thus, this work estimated the temperature distribution inside a member taking into consideration of the thermal properties by means of finite element method(FEM). The estimation results in a little higher prediction value than the experimental value in surface layer and is almost coincident with the experiment as the heating depth increase. From this work it can be known that the simulation application of FEM using the thermal properties of concrete member in high temperature gives rise to the confident prediction in the prediction of temperature distribution.

• Journal of Korean Neurosurgical Society
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• 제30권sup1호
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• pp.5-12
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• 2001

This study was designed to investigate the mechanism of cell death after cisplatin treatment in human glioblastoma A172 cells. Cis-diamminedichloroplatinum(Cisplatin) demonstrated cytostatic or cytotoxic effects on A172 cells in a dosedependent manner. Cisplatin-mediated cytotoxity in A172 cells was revealed as an apoptosis characterized by high molecular weight DNA fragmentation by agarose electrophoresis as well as nuclear fragmentation by Hoechst staining. Cisplatin also resulted in the activation of caspase 3-like protease as well as poly(ADP-ribose) polymerase(PARP) cleavage. Interestingly, the anti-apoptotic Bcl2 protein was degraded and furthermore, expression of p53 protein was increased by cisplatin in a time-dependent manner. Taken together, these results suggest that anticancer drug, cisplatin induces the apoptotic death of human glioblastoma A172 cells via the activations of caspase 3-like protease, degradation of anti-apoptotic Bcl2 protein and increase in the expression of p53.

• Nuclear Engineering and Technology
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• 제40권1호
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• pp.77-86
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• 2008

This paper describes the nonlinear analyses of a 1:4 scale model of a prestressed concrete containment vessel (PCCV) using an axisymmetric model and a three-dimensional model. These two models are refined by comparison of the analysis results and with testing results. This paper is especially focused on the analysis of behavior under pressure and the temperature effects revealed using an axisymmetric model. The temperature-dependent degradation properties of concrete and steel are considered. Both geometric and material nonlinearities, including thermal effects, are also addressed in the analyses. The Menetrey and Willam (1995) concrete constitutive model with non-associated flow potential is adopted for this study. This study includes the results of the predicted thermal and mechanical behaviors of the PCCV subject to high temperature loading and internal pressure at the same time. To find the effect of high temperature accident conditions on the ultimate capacity of the liner plate, reinforcement, prestressing tendon and concrete, two kinds of analyses are performed: one for pressure only and the other for pressure with temperature. The results from the test on pressurization, analysis for pressure only, and analyses considering pressure with temperatures are compared with one another. The analysis results show that the temperature directly affects the behavior of the liner plate, but has little impact on the ultimate pressure capacity of the PCCV.

• International Journal of Oral Biology
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• 제46권1호
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• pp.30-38
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• 2021

Cudraxanthone D (CD) is a natural xanthone compound derived from the root barks of Cudrania tricuspidata. However, the biological functions of CD in human metabolism have been rarely reported until now. Autophagy is the self-degradation process related to cancer cell metastasis. Here, we elucidated the effects of CD on human oral squamous cell carcinoma (OSCC) cells' metastatic ability. We confirmed that CD effectively decreased the proliferation and viability of SCC25 human OSCC cells in time- and dose-dependent manners. Also, the metastasis phenotype of the SCC25 cell (migration, invasion, and epithelial-mesenchymal transition [EMT]) was inhibited by CD. To further investigate the mechanism by which CD inhibited the metastatic capacity, we detected the relationship between EMT and autophagy in the SCC25 cells. The results revealed that CD inhibited the metastasis of the SCC25 cells by attenuating autophagy. Thus, our findings produced a potential novel agent for the treatment of human OSCC metastasis.