• Journal of Microbiology and Biotechnology
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• 제25권3호
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• pp.386-392
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• 2015

A novel recombinant bacmid, bEasyBm, that enables the easy and fast generation of pure recombinant baculovirus without any purification step was constructed. In bEasyBm, attR recombination sites were introduced to facilitate the generation of a recombinant viral genome by in vitro transposition. Moreover, the extracellular RNase gene from Bacillus amyloliquefaciens, barnase, was expressed under the control of the Cotesia plutellae bracovirus early promoter to negatively select against the nonrecombinant background. The bEasyBm bacmid could only replicate in host insect cells when the barnase gene was replaced with the gene of interest by in vitro transposition. When bEasyBm was transposed with pDualBac-EGFP, the resulting recombinant virus, EasyBm-EGFP, showed high levels of EGFP expression efficiency compared with that of non-purified recombinant virus BmGOZA-EGFP, which was constructed using the bBmGOZA system. In addition, nonrecombinant backgrounds were not detected in unpurified EasyBm-EGFP stocks. Based on these results, a high-throughput system for the generation of multiple recombinant viruses at a time was established.

• 대한조선학회논문집
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• 제39권1호
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• pp.49-60
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• 2002

용접법은 선박 생산 공정에 있어서 금속 접합을 위해 가장 많이 사용되고 있다. 용접은 금속 접합을 위해 유용한 방법이지만 국부적 가열, 용융, 냉각으로 이어지는 열하중에 의하여 잔류응력과 잔류변형을 발생시킨다. 잔류응력은 구조물의 강도에 부정적인 영향을 끼치고, 잔류변형은 조립 작업에 부정적인 영향을 끼치게 된다. 그러므로 역학적 방법에 의한 잔류응력과 잔류변형의 예측은 이들을 제어할 수 있는 방법을 작업 전에 제시할 수 있도록 하므로, 선체 강도 확보와 선박 생산성 향상을 위하여 매우 중요한 문제라고 볼 수 있다. 본 논문에서는 이러한 배경에서 용접 현상을 가장 잘 시뮬레이션할 수 있는 방법인 유한요소법을 이용한 3차원 열탄소성 해석을 이용하였다. 열탄소성 해석을 위하여 온도분포를 계산한 후 계산된 온도분포를 이용하여 용접변형 및 응력을 순차적으로 계산하였다. 온도분포 계산을 위하여 용융과정을 엔탈피 방법을 이용하여 구현하였으며, 용접비드의 용입은 요소생성법을 이용하여 구현하였다. 본 연구에서 제안된 해석과정은 기존 연구에서 제시한 실험결과와 정성적으로 일치하는 결과를 주는 것을 확인하였다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2005년도 ICCAS
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• pp.649-652
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• 2005

The camera cellular phone has a large portion of cellular phone market in recent year. The variety of a customer demand makes a fast model change and the spatial resolution is changed from VGA to multi-mega pixel. The 1.3 mega pixel (MP) camera cellular phone was first released into the Korean market in October 2003. The major cellular phone companies released a 2MP camera cellular phone that supports zoom function and a 2MP camera cellular phone is settled down with the Korea cellular phone market. It makes a keen competition in price and demands automation for phone camera module. There is an increasing requirement for the automatic assembly to correspond to a fast model change. The hard automation techniques that rely on dedicated manufacturing system are too inflexible to meet this requirement. Therefore in this study, this system is designed with the flexibility concept in order to cope with phone camera module change. The system has a same platform that has X-Y-Z motion or X-Z motion with ${\mu}m$order accuracy. It has a special gripper according to the type of a component to be put together. If the camera model changes, the gripper may be updated to fit for the camera module. The controller of this system acquires the data sets that have the information about the assembly part by the tray. This information is obtained ahead of an inspection step. The controller excludes an inferior part to be assembled by using this information to diminish the inferior goods. The assembly jig used in this system has a function of self adjustment that reduces the tact time and also diminish the inferior goods. Finally, the intelligent assembly system for phone camera module will be designed to get a flexibility to meet model change and a high productivity with a high reliability.

• Nuclear Engineering and Technology
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• 제52권8호
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• pp.1626-1637
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• 2020

This work covers an important point of the benchmark released by the expert group on Uncertainty Analysis in Modeling of Light Water Reactors. This ambitious benchmark aims to determine the uncertainty in light water reactors systems and processes in all stages of calculation, with emphasis on multi-physics (coupled) and multi-scale simulations. The Gesellschaft für Anlagen und Reaktorsicherheit methodology is used to propagate the thermal-hydraulic uncertainty of macroscopic parameters through TRACE5.0p3/PARCSv3.0 coupled code. The main innovative points achieved in this work are i) a new thermal-hydraulic model is developed with a highly-accurate 3D core discretization plus an iterative process is presented to adjust the 3D bypass flow, ii) a control rod insertion occurrence -which data is obtained from a real PWR test- is used as a transient simulation, iii) two approaches are used for the propagation process: maximum response where the uncertainty and sensitivity analysis is performed for the maximum absolute response and index dependent where the uncertainty and sensitivity analysis is performed at each time step, and iv) RESTING MATLAB code is developed to automate the model generation process and, then, propagate the thermal-hydraulic uncertainty. The input uncertainty information is found in related literature or, if not found, defined based on expert judgment. This paper, first, presents the Gesellschaft für Anlagen und Reaktorsicherheit methodology to propagate the uncertainty in thermal-hydraulic macroscopic parameters and, then, shows the results when the methodology is applied to a PWR reactor.

• Ocean and Polar Research
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• 제31권4호
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• pp.339-347
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• 2009

It is possible to obtain accurate temperature and salinity profiles of the oceans using a SBE 911plus CTD and accompanying data conversion packages. To obtain highly accurate results, CTD data needs to be carefully processed in addition to proper and regular maintenance of the CTD itself. Since the manufacturer of the CTD provides tools that are necessary for post processing, it is possible to conduct proper processing without too much effort. Some users, however, are not familiar with all of the processes and inadvertently ignore some of these processes at the expense of data quality. To draw attention to these and other similar issues, we show how it is possible to improve data quality by utilizing a few extra processes to the standard or default data process procedures with CTD data obtained from the equatorial Eastern Pacific between 2001 and 2005, and 2007. One easy step that is often ignored in the standard data process procedure is "wild edit", which removes abnormal values from the raw data. If those abnormal values are not removed, the abnormality could spread vertically during subsequent processes and produce abnormal salinity in a range much wider than that of the raw data. To remove spikes in salinity profiles the "align CTD" procedure must be carried out not with the default values included in the data processing software but with a proper time constant. Only when "cell thermal mass" correction is conducted with optimal parameters, we can reduce the difference between upcast and downcast, and obtain results that can satisfy the nominal accuracy of the CTD.

• 한국생활과학회지
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• 제24권6호
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• pp.755-768
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• 2015

The purpose of this study was to verify the mediating effects on self-regulated learning ability and sense of community in the relationship between parents' neglect and children's academic achievement. The subjects used in this study were 2,218 6th grade elementary school students from the third wave sample of the 2012 Korean Children and Youth Panel Survey (KCYPS). For data analysis, the three-step mediated regression analysis by Baron and Kenny (1986) was performed and the Sobel test was carried out in order to verify the effectiveness of mediation effects. The main results of the present study were as follows. First, the self-regulated learning ability and sub-component factors of achievement value, mastery goal orientation, behavioral control, academic time-management revealed to have a partial mediation effect in the relationship between parents' neglect and children's academic achievement. Second, the sense of community also showed to have a partial mediation effect on the relationship between parents' neglect and children's academic achievement. The findings of this study provide a viewpoint to deeply observe the problem of parents' neglect in connection with children's self-regulated learning ability and sense of community, and can be used as practical data to develop various programs for the benefit of improving children's academic achievement.

• 한국정보처리학회논문지
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• 제5권5호
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• pp.1345-1356
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• 1998

본 논문은 컴파일러에 의해 생성된 목적 코드상의 프로시저의 수행 속도를 향상시키기 위한 방안으로, 프롤로그 테일러링 알고리즘을 제안한다. 레지스터의 수가 많은 기계에서 반복 수행되는 프로시저의 경우, 프롤로그와 에필로그에서 실행되는 레지스터 저장 및 복원 명령어를 줄이는 것은 프로시저 실행 속도 향상의 주요 작업이 된다. IBM XL C 컴파일러에서 제공되는 에필로그 테일러링은 실행 경로상의 레지스터 복원 명령어를 줄임으로써, 프로시저 성능 향상에 기여해왔으나, 프롤로그 테일러링에 대한 구체적 알고리즘은 현재 제안되어 있지 않다. 본 논문이 제안하는 알고리즘에 의해 생성된 프롤로그는 각각의 실행 경로에 대해 현저히 감소된 수의 레지스터 저장 명령을 실행하도록 함으로써, 프로시저의 실행 속도를 개선시킨다. 테일러된 프롤로그를 갖는 프로시저가 바르게 실행되기 위해서는, 다이아몬드 구조나 반복 구조 내분에 레지스터 저장 명령을 생성해서는 안된다. 그러므로, 본 논문은 다이아몬드 구조나 반복 구조 내부가 아닌 최적의 위치에 레지스터 저장 명령을 생성하는 알고리즘을 제안한다.

• 화약ㆍ발파
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• 제22권4호
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• pp.7-15
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• 2004

본 연구에서는 PFC3D를 사용한 폭원모델링 기법을 제안하고, 제안된 기법을 시멘트 모르타르와 같은 연약재료의 발파에 적용하여 그 적용성을 시험해 보았다. PFC3D는 개별요소법(DEM)을 기반으로 하고 있어 응력파의 전파와 재료의 동적 파괴현상을 모사하는데 적합한 코드로 분류된다. 폭원모델링 과정에서는 공내입자들의 반경을 팽창/수축시키는 기법을 통해 공벽입자들에 접촉력의 형태로 폭발압력을 부여하는 방법을 사용하였으며, 입력하중에 따라 공벽에서 유발되는 접촉력을 계산단계마다 측정 및 보정함으로써 폭발압력의 크기를 제어할 수 있도록 하였다. 시멘트 모르타르 블록의 발파모델링 과정에서는 기존의 외력을 이용하는 방법과 본 연구에서 제안하고 있는 접촉력을 이용하는 기법을 각기 적용함으로써 연약재료의 파괴과정을 정성적으로 비교하여 보았다. 해석결과, 제안된 폭원모델링 기법을 적용한다면 암석이나 콘크리트와 같은 공학재료들이 발파과정에서 보이는 파괴거동을 수치적으로 보다 유사하게 모사 할 수 있을 것으로 판단된다.

• KSBB Journal
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• 제17권4호
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• pp.326-332
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• 2002

크로마토그래피 공정 성능 검증의 한 항목인 담체의 수명 검증을 위해 자동화된 미니 크로마토그래피 시스템(MiniValChrom)을 개발하였다. 이 시스템은 크로마토그래피공정의 자동화된 반복수행, 크로마토그래피 각 step의 작동순서 및 공정조건의 자유로운 구성, on-line 실시간 모니터링 및 제어, 여러 담체 수명 검증법의 method file 저장기능 등을 갖추었다. MiniValChrom을 사용하여 BSA와 Cibacron Blue 3G-A를 각각 모델 단백질과 담체로 담체 수명 검증실험을 사례연구로 수행하였다. 담체 수명의 감소는 크로마토그래피 공정을 반복수행하면서 5 cycle 마다 변화하는 HETP값을 측정하여 HETP 값이 1 cm 이상 될 때까지 반복 수행함으로써 담체 수명을 결정할 수 있었다. 본 연구를 통해 개발된 MiniValChrom은 다른 검증 항목이나 multi-product 생산공장 내 크로마토그래피 공정 검증에도 유용하게 쓰일 수 있을 것으로 기대된다.

• 한국분무공학회지
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• 제25권1호
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• pp.8-14
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• 2020

Recently, 1-D model-based engine development using virtual engine system is getting more attention than experimental-based engine development due to the advantages in time and cost. Injection rate profile is the one of the main parameters that determine the start and end of combustion. Therefore, it is essential to set up a sophisticated model to accurately predict the injection rate as starting point of virtual engine system. In this research, procedure of 1-D model setup based on AMESim is introduced to predict the dynamic behavior and injection rate of diesel injector. As a first step, detailed 3D cross-sectional drawing of the injector was achieved, which can be done with help of precision measurement system. Then an approximate AMESim model was provided based on the 3D drawing, which is composed of three part such as solenoid part, control chamber part and needle and nozzle orifice part. However, validation results in terms of total injection quantity showed some errors over the acceptable level. Therefore, experimental work including needle movement visualization, solenoid part analysis and flow characteristics of injector part was performed together to provide more accuracy of 1-D model. Finally, 1-D model with the accuracy of less than 10% of error compared with experimental result in terms of injection quantity and injection rate shape under normal temperature and single injection condition was established. Further work considering fuel temperature and multiple injection will be performed.