• Journal of Microbiology and Biotechnology
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• v.25 no.3
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• pp.386-392
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• 2015

A novel recombinant bacmid, bEasyBm, that enables the easy and fast generation of pure recombinant baculovirus without any purification step was constructed. In bEasyBm, attR recombination sites were introduced to facilitate the generation of a recombinant viral genome by in vitro transposition. Moreover, the extracellular RNase gene from Bacillus amyloliquefaciens, barnase, was expressed under the control of the Cotesia plutellae bracovirus early promoter to negatively select against the nonrecombinant background. The bEasyBm bacmid could only replicate in host insect cells when the barnase gene was replaced with the gene of interest by in vitro transposition. When bEasyBm was transposed with pDualBac-EGFP, the resulting recombinant virus, EasyBm-EGFP, showed high levels of EGFP expression efficiency compared with that of non-purified recombinant virus BmGOZA-EGFP, which was constructed using the bBmGOZA system. In addition, nonrecombinant backgrounds were not detected in unpurified EasyBm-EGFP stocks. Based on these results, a high-throughput system for the generation of multiple recombinant viruses at a time was established.

• Journal of the Society of Naval Architects of Korea
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• v.39 no.1
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• pp.49-60
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• 2002

Welding is essential in ship production since welding is very popular method for joining two or more metals. However, welding causes residual stress and distortion and these give a bad influence to the structure strength and assembly of ship blocks. Therefore, prediction and treatment of residual stress and distortion is a key to accuracy control in shipyard. In this paper, a computational procedure, based on thermal-elastic-plastic 3-dimensional FEA, has been suggested to simulate butt and fillet welding process. In the simulation process, temperature distribution at each time step is obtained by heat transfer analysis and then thermal deformation analysis is done with obtained temperature distributions to find the residual stress and distortion. In heat transfer analysis, enthalpy method is used to realize phase change at melting temperature. Also element birth and death method is used to simulate adding of weld metal in both heat transfer analysis and thermal elastic plastic analysis. The proposed procedure is verified by related researches and the results show good agreement with those of related researches.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.649-652
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• 2005

The camera cellular phone has a large portion of cellular phone market in recent year. The variety of a customer demand makes a fast model change and the spatial resolution is changed from VGA to multi-mega pixel. The 1.3 mega pixel (MP) camera cellular phone was first released into the Korean market in October 2003. The major cellular phone companies released a 2MP camera cellular phone that supports zoom function and a 2MP camera cellular phone is settled down with the Korea cellular phone market. It makes a keen competition in price and demands automation for phone camera module. There is an increasing requirement for the automatic assembly to correspond to a fast model change. The hard automation techniques that rely on dedicated manufacturing system are too inflexible to meet this requirement. Therefore in this study, this system is designed with the flexibility concept in order to cope with phone camera module change. The system has a same platform that has X-Y-Z motion or X-Z motion with ${\mu}m$order accuracy. It has a special gripper according to the type of a component to be put together. If the camera model changes, the gripper may be updated to fit for the camera module. The controller of this system acquires the data sets that have the information about the assembly part by the tray. This information is obtained ahead of an inspection step. The controller excludes an inferior part to be assembled by using this information to diminish the inferior goods. The assembly jig used in this system has a function of self adjustment that reduces the tact time and also diminish the inferior goods. Finally, the intelligent assembly system for phone camera module will be designed to get a flexibility to meet model change and a high productivity with a high reliability.

• Nuclear Engineering and Technology
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• v.52 no.8
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• pp.1626-1637
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• 2020

This work covers an important point of the benchmark released by the expert group on Uncertainty Analysis in Modeling of Light Water Reactors. This ambitious benchmark aims to determine the uncertainty in light water reactors systems and processes in all stages of calculation, with emphasis on multi-physics (coupled) and multi-scale simulations. The Gesellschaft für Anlagen und Reaktorsicherheit methodology is used to propagate the thermal-hydraulic uncertainty of macroscopic parameters through TRACE5.0p3/PARCSv3.0 coupled code. The main innovative points achieved in this work are i) a new thermal-hydraulic model is developed with a highly-accurate 3D core discretization plus an iterative process is presented to adjust the 3D bypass flow, ii) a control rod insertion occurrence -which data is obtained from a real PWR test- is used as a transient simulation, iii) two approaches are used for the propagation process: maximum response where the uncertainty and sensitivity analysis is performed for the maximum absolute response and index dependent where the uncertainty and sensitivity analysis is performed at each time step, and iv) RESTING MATLAB code is developed to automate the model generation process and, then, propagate the thermal-hydraulic uncertainty. The input uncertainty information is found in related literature or, if not found, defined based on expert judgment. This paper, first, presents the Gesellschaft für Anlagen und Reaktorsicherheit methodology to propagate the uncertainty in thermal-hydraulic macroscopic parameters and, then, shows the results when the methodology is applied to a PWR reactor.

• Ocean and Polar Research
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• v.31 no.4
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• pp.339-347
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• 2009

It is possible to obtain accurate temperature and salinity profiles of the oceans using a SBE 911plus CTD and accompanying data conversion packages. To obtain highly accurate results, CTD data needs to be carefully processed in addition to proper and regular maintenance of the CTD itself. Since the manufacturer of the CTD provides tools that are necessary for post processing, it is possible to conduct proper processing without too much effort. Some users, however, are not familiar with all of the processes and inadvertently ignore some of these processes at the expense of data quality. To draw attention to these and other similar issues, we show how it is possible to improve data quality by utilizing a few extra processes to the standard or default data process procedures with CTD data obtained from the equatorial Eastern Pacific between 2001 and 2005, and 2007. One easy step that is often ignored in the standard data process procedure is "wild edit", which removes abnormal values from the raw data. If those abnormal values are not removed, the abnormality could spread vertically during subsequent processes and produce abnormal salinity in a range much wider than that of the raw data. To remove spikes in salinity profiles the "align CTD" procedure must be carried out not with the default values included in the data processing software but with a proper time constant. Only when "cell thermal mass" correction is conducted with optimal parameters, we can reduce the difference between upcast and downcast, and obtain results that can satisfy the nominal accuracy of the CTD.

• Korean Journal of Human Ecology
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• v.24 no.6
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• pp.755-768
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• 2015

The purpose of this study was to verify the mediating effects on self-regulated learning ability and sense of community in the relationship between parents' neglect and children's academic achievement. The subjects used in this study were 2,218 6th grade elementary school students from the third wave sample of the 2012 Korean Children and Youth Panel Survey (KCYPS). For data analysis, the three-step mediated regression analysis by Baron and Kenny (1986) was performed and the Sobel test was carried out in order to verify the effectiveness of mediation effects. The main results of the present study were as follows. First, the self-regulated learning ability and sub-component factors of achievement value, mastery goal orientation, behavioral control, academic time-management revealed to have a partial mediation effect in the relationship between parents' neglect and children's academic achievement. Second, the sense of community also showed to have a partial mediation effect on the relationship between parents' neglect and children's academic achievement. The findings of this study provide a viewpoint to deeply observe the problem of parents' neglect in connection with children's self-regulated learning ability and sense of community, and can be used as practical data to develop various programs for the benefit of improving children's academic achievement.

• The Transactions of the Korea Information Processing Society
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• v.5 no.5
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• pp.1345-1356
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• 1998

Prolog tailoring technique, an optimization method to improve the execution speed of a procedure, is proposed in this paper. When a procedure is frequently and repeatedly called and the machine has a lot of callee-saved registers, optimizing prolog and epilog can become an important step of optimization. Epilg tailoring supported by IBM XL C Compiler has been known to improve procedure's execution speed by reducing register restore instructions on execution paths, but no algorithms for prolog tailoring has been proposed yet. The prolog generated by the prolog tailoring algorithm proposed in this paper executes considerably smaller number of register save instructions at run-time. This means the total number of instructions to be executed is decreased resulting in an improvement on the procedure's execution speed. To maintain the correctness of code, prolog code should not be inserted inside diamond structures of loop structures. This paper proposes a prolog tailoring technique which generates register save instructions at the best position in a control flow graph while not allowing the insertion of any prolog code inside diamond structures of loop structures.

• Explosives and Blasting
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• v.22 no.4
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• pp.7-15
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• 2004

An explosion modeling technique was developed by using the spherical discrete element code, PFC3D, which can be used to model the dynamic stress wave propagation phenomenon. The modeling technique is simply based on an idea that the explosion pressure should be applied to a PFC3D particle assembly not in the form of an external force (body force), but in the form of a contact force (surface force). According to this concept, the explosion pressure is applied to the wall particles by the scheme of radius expansion/contraction of inner-hole particles. The output wall force is compared to the input hole pressure in every time step, and a correction routine is activated to control the radius multiplier of the inner-hole particles. A comparative blast simulation far a cement mortar block of $80\times90\times80mm$ was conducted by using the conventional explosion modeling method and the new one. The results of the simulation are presented in a qualitative fashion.

• KSBB Journal
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• v.17 no.4
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• pp.326-332
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• 2002

The quality of biopharmaceutical proteins is strongly affected by a manufacturing process employed to produce Et, and thus validation of the manufacturing bioprocess is a very important issue. Chromatography is probably the most widely used bioprocess unit operation for protein purification. In this study, a miniaturized automatic chromatography system was designed and constructed for scale-down studies for process chromatography validation. This system, named MiniValChrom, has the following features: automatic and repeated operation, flexible sequences and intervals among the steps, on-line and real-time monitoring and control, method files savings, etc. Using the MiniValChrom, we peformed a case study of an abbreviated experiment to estimate chromatographic resin lifetime. BSA (bovine serum albumin) and Cibacron Blue 3G-A were used as the model protein and the resin, respectively. The resin deterioration was evaluated by determining and monitoring the HETP and NTP values from the chromatograms every 5 cycles. It was observed that the HETP and the NTP values were changed by 9% after 15 cycles. The resin lifetime validation could be completed by repeating this experiment until the HETP value reached a predetermined value. The MiniValChrom's concept and the protocol suggested in this study can serve as a rapid and economical tool for the validation studies of bioprocess chromatography system.

• Journal of ILASS-Korea
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• v.25 no.1
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• pp.8-14
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• 2020

Recently, 1-D model-based engine development using virtual engine system is getting more attention than experimental-based engine development due to the advantages in time and cost. Injection rate profile is the one of the main parameters that determine the start and end of combustion. Therefore, it is essential to set up a sophisticated model to accurately predict the injection rate as starting point of virtual engine system. In this research, procedure of 1-D model setup based on AMESim is introduced to predict the dynamic behavior and injection rate of diesel injector. As a first step, detailed 3D cross-sectional drawing of the injector was achieved, which can be done with help of precision measurement system. Then an approximate AMESim model was provided based on the 3D drawing, which is composed of three part such as solenoid part, control chamber part and needle and nozzle orifice part. However, validation results in terms of total injection quantity showed some errors over the acceptable level. Therefore, experimental work including needle movement visualization, solenoid part analysis and flow characteristics of injector part was performed together to provide more accuracy of 1-D model. Finally, 1-D model with the accuracy of less than 10% of error compared with experimental result in terms of injection quantity and injection rate shape under normal temperature and single injection condition was established. Further work considering fuel temperature and multiple injection will be performed.