• Title/Summary/Keyword: Time Determination

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Effect of Thresher Drum-Speed on the Quality of the Milled Rice (탈곡기의 급동 속도가 도정 손실에 미치는 영향)

  • Chung, Chang Joo;Koh, Hak Kyun;Lee, Chong Ho;Kang, Hwa Seug
    • Journal of Biosystems Engineering
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    • v.4 no.2
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    • pp.9-9
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    • 1979
  • It is understood that drum speed of threshers and the moisture content of paddy grains to be threshed, respectively, have a signific:mt effect upon rice recoveries. Threshing under an increased drum speed would give a high performance rate, which is the general practice in custom work threshing in association with the use of semiauto-t hreshers. In the connection, however, it may result in the promotion of grain cracks and brokens of the rice product after milling. No reference or determination for an opti mum drum speed of the thresher is made available for various grain moisture contents at the time of the threshing operation and for different rice varieties especially for the Tongil rice varieties. This study was Conducted to find out and determine effects of the drum speeds on grain losses. The grain loss was quantified in terms of recovery rates of rice grains after treatments. Samples of each of all treatments were taken from the grain sampling plate placed in the grain conveyor of threshers. The grain sample plate was specially provided for this experiment. The brown-rice, milling, and head-rice recJveries were tes ted in the laboratory mill, respectively. Two rice varieties, Akibare and Suweon 251, each with five levels of different moist?ure contents at harvest and six levels of different drum speeds of threshers, were selected and used for treatments in this experiment. Two conditions of materials were tested in the thresher. One condition was to thresh the experimental material immediately after cutting, referred to as the wet-material thr eshing in this study. The other was to thresh the experimental :material, dried to contain about 15-16 percent of the grain moisture under the shocking operation. This is referred to as the dry-material threshing in this study. In additioon, field measurements for the grain moistures and drum-sdeeds under actual operation practices of the traditional field threshing, were conducted with a view to comparing with results of the experimental treatments. The results of the study may be summarized as follows: 1. For threshing treatments of Japonica-type rice variety (Akibare) , the effect of drum speeds and levels of grain moisture at cutting upon brown-rice, milling, and head-rice recoveries were found statistically significant. No significant difference in these recovery rates was noticed regardless of whether the material was threshed right after cutting or after drying by the shocking operation. 2. For the Tongil-sister rice variety(Suweon 251), milling recovery for the varied drum-speed and the grain~moisture level at cutting was found statististically significant. Th milling recovery was much significant when associated with the wet-material thres?hing compared to the dry-material threshing. 3. The optimum peripheral velocity to be maintained at the edge of teeth on the thr?esher drum was determined and may be recommanded as that of about 12 to 13 meters per second in view of the maximum recovery rate of the milled rice. 4. The effect of the drum speed on the qualitative loss of the milled rice was much greater in the case of the Tongil variety than Japonica. This effect was also greater by the wet-material threshing than by the dry-material threshing. Therefore, to apply the wet-material threshing operation for the Tongil variety, in particular, it should be very important to introduce the kind of threshing technology which would maintain the drum speed at optimum. 5. A field survey for the actual drum speed of threshing operations for 50 threshers indicated that average peripheral velccity was 12.76m/sec., and that the range was from 10.50 to 14.90m/sec. Approximately, more than 30% of the experimented and measured threshers were being operated at speeds which exceeded the optimum speed determined and assessed in this study. Accordingly, it should be highly desirable and important to take counter-measures against these threshing practices of operational overspeed.

Detecting Phenology Using MODIS Vegetation Indices and Forest Type Map in South Korea (MODIS 식생지수와 임상도를 활용한 산림 식물계절 분석)

  • Lee, Bora;Kim, Eunsook;Lee, Jisun;Chung, Jae-Min;Lim, Jong-Hwan
    • Korean Journal of Remote Sensing
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    • v.34 no.2_1
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    • pp.267-282
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    • 2018
  • Despite the continuous development of phenology detection studies using satellite imagery, verification through comparison with the field observed data is insufficient. Especially, in the case of Korean forests patching in various forms, it is difficult to estimate the start of season (SOS) by using only satellite images due to resolution difference. To improve the accuracy of vegetation phenology estimation, this study reconstructed the large scaled forest type map (1:5,000) with MODIS pixel resolution and produced time series vegetation phenology curves from Normalized Difference Vegetation Index (NDVI) and Enhanced Vegetation Index (EVI) derived from MODIS images. Based on the field observed data, extraction methods for the vegetation indices and SOS for Korean forests were compared and evaluated. We also analyzed the correlation between the composition ratio of forest types in each pixel and phenology extraction from the vegetation indices. When we compared NDVI and EVI with the field observed SOS data from the Korea National Arboretum, EVI was more accurate for Korean forests, and the first derivative was most suitable for extracting SOS in the phenology curve from the vegetation index. When the eight pixels neighboring the pixels of 7 broadleaved trees with field SOS data (center pixel) were compared to field SOS, the forest types of the best pixels with the highest correlation with the field data were deciduous forest by 67.9%, coniferous forest by 14.3%, and mixed forest by 7.7%, and the mean coefficient of determination ($R^2$) was 0.64. The average national SOS extracted from MODIS EVI were DOY 112.9 in 2014 at the earliest and DOY 129.1 in 2010 at the latest, which is about 0.16 days faster since 2003. In future research, it is necessary to expand the analysis of deciduous and mixed forests' SOS into the extraction of coniferous forest's SOS in order to understand the various climate and geomorphic factors. As such, comprehensive study should be carried out considering the diversity of forest ecosystems in Korea.

Prediction of Nitrate Contamination of Groundwater in the Northern Nonsan area Using Multiple Regression Analysis (다중 회귀 분석을 이용한 논산 북부 지역 지하수의 질산성 질소 오염 예측)

  • Kim, Eun-Young;Koh, Dong-Chan;Ko, Kyung-Seok;Yeo, In-Wook
    • Journal of Soil and Groundwater Environment
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    • v.13 no.5
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    • pp.57-73
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    • 2008
  • Nitrate concentrations were measured up to 49 mg/L (as $NO_3$-N) and 22% of the samples exceeded drinking water standard in shallow and bedrock groundwater of the northern Nonsan area. Nitrate concentrations showed a significant difference among land use groups. To predict nitrate concentration in groundwater, multiple regression analysis was carried out using hydrogeologic parameters of soil media, topography and land use which were categorized as several groups, well depth and altitude, and field parameters of temperature, pH, DO and EC. Hydrogeologic parameters were quantified as area proportions of each category within circular buffers centering at wells. Regression was performed to all the combination of variables and the most relevant model was selected based on adjusted coefficient of determination (Adj. $R^2$). Regression using hydrogelogic parameters with varying buffer radii show highest Adj. $R^2$ at 50m and 300m for shallow and bedrock groundwater, respectively. Shallow groundwater has higher Adj. $R^2$ than bedrock groundwater indicating higher susceptibility to hydrogeologic properties of surface environment near the well. Land use and soil media was major explanatory variables for shallow and bedrock groundwater, respectively and residential area was a major variable in both shallow and bedrock groundwater. Regression involving hydrogeologic parameters and field parameters showed that EC, paddy and pH were major variables in shallow groundwater whereas DO, EC and natural area were in bedrock groundwater. Field parameters have much higher explanatory power over the hydrogeologic parameters suggesting field parameters which are routinely measured can provide important information on each well in assessment of nitrate contamination. The most relevant buffer radii can be applied to estimation of travel time of contaminants in surface environment to wells.

Study on the Relationship between Biliary Secretion and Cyclic Nucleotides (담즙분비와 Cyclic nucleotides간의 상호관계에 관한 연구)

  • Lee, H.W.;Kim, W.J.;Hong, S.S.;Cho, S.J.;Hong, S.U.;Lim, C.K.
    • The Korean Journal of Pharmacology
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    • v.18 no.1
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    • pp.43-54
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    • 1982
  • Bile formation is a complex process comprised of three separate physiologic mechanism operating at two anatomical sites. At present time, it was known that at least two processes are responsible for total canalicular secretion at the bile canaliculus. One of the processes is bile salt-dependent secretion (BSDS) hypothesis that the active transport of bile salts from plasma to bile provided a primary stimulus for bile formation: the osmotic effect of actively transported bile acid was responsible for the movement of water and ions into bile. The other process is bile salt-independent secretion (ESIS), which is unrelated to bile salt secretion at the canaliculus and which may involve the active transport of sodium. The third process for bile formation involves the biliary ductal epithelium. Secretin-stimulated bile characteristically contained bicarbonate in high concentration. Therefor, it was suggested that secretin stimulated water and bicarbonate secretion from the biliary ductules. One the other hand, it was found that a large amounts of cAMP was present in canine bile but no apparent relationship between bile salt secretion and cAMP content in dog bile. However, bile flow studies in human have demonstrated that secretin and glucagon increase bile cAMP secretion as does secretin in baboons. Secretin increases baboon bile duct mucosal cAMP levels in addition to bile CAMP levels suggesting that in that species secretin-stimulated bile flow may be cAMP mediated. It has been postulated that glucagon and theophylline which increase the bile salt-independent secretion in dogs might act through an increased in liver cAMP content. In a few studies, the possible role of cAMP on bile formation has teen tested by administration of an exogenous derivative of cAMP, dibutyryl cAMP. In the rat, DB cAMP did not modify bile flow, but injection of DB cAMP in the dog promoted an increase in the bile salt-independent secretion. Because of these contradictory results, this study was carried out to examine the relationship between cyclic nucleotides and bile flow due to various bile salts as well as secretin or theophylline. Experiments were performed in rabbits with anesthesia produced by the injection of seconal(30 mg/kg). Rabbits had the cystic duct ligated and the proximal end of the divided common duct cannulated with an appropriately sized polyethylene catheter. A similar catheter was placed into the inferior vena cava for administration of drugs. Bile was collected for determination of cyclic nucleotides and total cholate in 15 min. intervals for a few hours. The results are summerized as followings. 1) Administrations of taurocholic acid or chenodeoxycholic acid increased significantly the concentrations of cAMP and cGMP in bile of rabbits. 2) Concentration of cAMP in bile during the continuous infusion of ursodeoxycholic acid, was remarkedly increased in accordance with the increase of bile flow, while on the contrary concentration of cGMP in bile was decreased significantly. 3) Dehydrocholic acid and deoxycholic acid significantly increased bile flow, total cholate output and cyclic nucleotides in bile. 4) Only cAMP concentration in bile was significantly increased from control value by secretin, while theophylline increased cAMP as well as cGMP in rabbit bile. 5) In addition, the administration of secretin to taurocholic acid-stimulated bile flow increased cAMP while theophylline produced the increases of cAMP and cGMP in bile. 6) The administration of insulin to taurocholic acid-stimulated bile flow decreased cAMP concentration, while on the contrary cGMP was remarkedly increased in rabbit bile.

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Research on Mechanized Upland Reclamation Works An Experiment for the Selection of Optimum Teeth Interval and the Analysis of Efficiency of Stumping and Root-Clearing Methods by Rake Dozer. (Report 1) (야산 기계화 개간공법에 관한 연구 -레이크 도저의 적정 이빨 간격의 선택과 발배근작업능률 시험(제1보)-)

  • 류한열;정하우박승우
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.17 no.3
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    • pp.3860-3871
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    • 1975
  • This experiment was carried out to establish the mechanized methods in stumping and root-clearing, which were the most important works in the reclamation of sloping uplands. The determination of optimum teeth interval of rake blades and its operation methods to reduce the quantity of transported topsoil during the works, are the aims of this investigation. A newly designed rake blade, whose net teeth intervals could be regulated by three stages as 15cm, 25cm, and 35cm, was manufactured to attach to the bulldozer of 13ton. The experiments were carried out at Kilsang-Myon, Kwangwha-Gun, from Aug. 9 to Aug. 23, 1975. For each interval, 36 test plots of 50${\times}$10mn in size, which were regulated under three levels of land slopes of 10, 20, and 30% and two different tree stand density of high or medium values, were randomly chosen and arranged by two-replicated split-split plot design. Each stump classified by its diameter was stumped and cleared by the rake dozer to be related between diameter and stumping time. The results obtained in this experiment can be summarized as follows: 1. Stumping times for the diameters ranging from 6 to 18cm of stumps are almost the same and they are not varied by the difference of teeth intervals of rake dozer. 2. By back-ward stumping method, the number of stumps which can be stumped per hour ranges almost from 100 to 170, showing significant difference with respect to the teeth intervals. The working area is sharply varied with not only the stand density of stumps but the teeth intervals. 3. Optimum stumping distance for each teeth interval of rake dozer to minimize the quantity of transported topsoil are varied with such the rates as it is 15m or 20m for 15cm of teeth interval, but 25m for 25cm or 35cm, respectively. The clearing distance could be chosen almost double as long as the operating distance. 4. The working areas per hour of the simultaneous stumping and clearing methods are no significant difference among the various treatments of working conditions, but they are affected by the operating techniques. However, the influencing factors of the working conditions as classified before and the working directions are ranged from 10 to 15 per cent of total working area, respectively. 5. The residual rates of stumps which are not stumped by the rake dozer in each test plot are generally reduced as the teeth interval gets narrower, but there are no significant difference among them. The mean residual rates average to be about 4% for the simultaneous stumping and clearing method. The back-ward stumping method are recommended to be supervised and directed by more than one man, to show the operator where the stumps are located. 6. The results according to the stumping and clearing methods are summarized as Table IV-2. And the selection of working methods is recommended to follow as shown in Fig. IV-9 with respect to the stand density of the field. 7. Generally speaking, the narrower the teeth interval, the better become the working results, but the more the quantity of transported topsoil is increased. Therefore, it is recommended that the teeth interval should be reduced from the present distance of more than 30cm to 25cm or 15cm, by developing suitable working methods through more field works and experiments.

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A Comparative Analysis of Blood Estradiol-17${\beta}$ Levels According to Varying Regimens of $Clomid^{(R)}$(Clomiphene citrate) Administration in Induced Ovulated Menstrual Cycles ($Clomid^{(R)}$(Clomiphene citrate)의 투여시기와 용량을 변수로 한 혈중 $Estradiol-17{\beta}$의 동태에 관한 연구)

  • Chang, H.J.;Suh, B.H.;Lee, J.H.
    • Clinical and Experimental Reproductive Medicine
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    • v.15 no.1
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    • pp.40-52
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    • 1988
  • Estradiol-17${\beta}$($E_2$) levels in the blood were estimated according to varying the time and amount of the administration of $Clomid^{(R)}$. $Clomid^{(R)}$ were administered on the 2nd, 3rd and 4th menstrual day corresponding to the recruitment period and on the 5th menstrual day corresponding to the selection period of the ovarian follicles, respectively. And $Clomid^{(R)}$ were administered 50 mg, 100 mg and 150 mg/day, repectively. The effects of the sequential HMG to $E_2$ levels in the blood were also estimated. The results were as following : 1. Blood $E_2$ levels according to the day and amount of administration of $Clomid^{(R)}$ were the highest in the group 3(D $2{\sim}6$, 150 mg/day, with HMG) and the lowest in the group 6(D $5{\sim]9$, 50 mg/day, without HMG). $E_2$ levels showed increasing tendency to 0 day. 2. In the cases of the administration of $Clomid^{(R)}$ during the $2nd{\sim}6th$ menstrual day, $E_2$ levels according to the amount were similar among groups and showed increasing tendency daily. 3. In the cases of administration of $Clomid^{(R)}$ during the $2nd{\sim}6th$ menstrual day, $E_2$ levels according to the sequential HMG independent of the amount of $Clomid^{(R)}$ were higher in the with HMG group than without HMG groups. 4. In the case of the administration of $Clomid^{(R)}$ during the $5th{\sim}9th$ menstrual day, $E_2$ levels according to the amount were the highest in the 100 mg/day group and the lowest in the 50mg/day group. 5. In the cases of administration of $Clomid^{(R)}$ independent of the amount during the 5th${\sim}$9th menstrual day, $E_2$ levels according to the sequential HMG were higher in the with HMG group than without HMG group. 6. $E_2$ levels according to the amount independent of the day of the administration of $Clomid^{(R)}$ were the highest in the 100 mg/day group and 150 mg/day, 50 mg/day group in low sequence. 7. $E_2$ levels according to the sequential HMG independent of the day and amount of the administration of $Clomid^{(R)}$ were higher in the with HMG group than the without HMG group. 8. $E_2$ levels according to the day of the administration of $Clomid^{(R)}$ independent of the amount of $Clomid^{(R)}$ and sequential HMG were the highest in the group D 2${\sim}$6 and the lowest in the group D 5${\sim}$9. According to the above results, there were higher $E_2$ levels in the group with sequential HMG than without HMG. Therefore, the hypothesis, postulated initially by the author, was not verified that sequential HMG would not affect the $E_2$ levels which were related to the process of the selection of the ovarian follicle in the connection with 'FSH window'. Because it may be the stimulation after the selection of later predominant follicle. And the highest level of $E_2$ was estimated in the $Clomid^{(R)}$ 150 mg/day group with sequential HMG on the 2nd${\sim}$6th day, and the higher levels were estimated in the 2nd${\sim}$6th day, 3rd${\sim}$7th day and 4th${\sim}$8th day groups than the 5th${\sim}$9th day group. The lower levels were estimated in the $Clomid^{(R)}$ 50 mg/day group without HMG than 100 mg/day and 150 mg/day on the 5th${\sim}$9th day. Therefore, further study will be needed that combines analyses of the E2 levels in the blood according to the various administration of $Clomid^{(R)}$ with or without sequential HMG and determination of the numbers and size of the ovarian follicles by ultrasonogram.

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EFFECT OF SOFT-START CURING ON THE CONTRACTION STRESS OF COMPOSITE RESIN RESTORATION POLYMERIZED WITH LED AND PLASMA CURING UNIT (LED와 플라즈마 광원의 완속기시 광중합 방식이 복합레진의 수축응력에 미치는 영향)

  • Chung, Yang-Seok;Lee, Nan-Young;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.34 no.4
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    • pp.623-631
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    • 2007
  • Effect of Soft-start curing on the contraction stress of composite resin restoration polymerized with LED and plasma curing unit The purpose of this study was to evaluate the influence of soft-start light curing on contraction stress and hardness of composite resin. Composite resin (Filtek $Z-250^{TM}$, 3M ESPE, USA) was cured using the one-step continuous curing method with three difference light sources ; conventional halogen light ($XL3000^{TM}$, 3M ESPE, USA) cure for 40 seconds at $400 mw/cm^2$, LED light (Elipar Freelight $2^{TM}$, 3M-ESPE, USA) cure for 20 seconds at $800\;mW/cm^2$ a and plasma arc light ($Flipo^{TM}$, LOKKI, France) cure for 12 seconds at $1300 mW/cm^2$. For the soft-start curing method ; LED light (Elipar Freelight $2^{TM}$, 3M-ESPE, USA) cure exponential increase with 5 seconds followed by 17 seconds at $800\;mW/cm^2$ and plasma arc light ($Flipo^{TM}$, LOKKI, France) cure 2 seconds light exposure at $650\;mW/cm^2$ followed by 11 seconds at $1300\;mW/cm^2$. The strain guage method was used for determination of polymerization contraction. Measurements were recorded at each 2 second for the total of 800 seconds including the periods of light application. Obtained data were analyzed statically using Repeated measures ANOVA, One way ANOVA, and Tukey test. The results of present study can be summarized as follows: 1. Composite resin restoration showed transient expansion just after irradiation of curing light. Contraction stress was increased rapidly at the early phase of polymerization and reduced slowly as time elapsed (P<0.05). 2. Contraction stress was not revealed significant difference between Halogen curing light groups and LED and Plasma Light curing with soft-start group (P>0.05). 3. LED and Plasma Light curing with soft-start showed lower contraction stress than the one-step continuous light curing (P<0.05).

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Expiration-Day Effects: The Korean Evidence (주가지수 선물과 옵션의 만기일이 주식시장에 미치는 영향: 개별 종목 분석을 중심으로)

  • Choe, Hyuk;Eom, Yun-Sung
    • The Korean Journal of Financial Management
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    • v.24 no.2
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    • pp.41-79
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    • 2007
  • This study examines the expiration-day effects of stock index futures and options in the Korean stock market. The so-called 'expiration-day effects', which are the abnormal stock price movements on derivatives expiration days, arise mainly from cash settlement. Index arbitragers have to bear the risk of their positions unless they liquidate their index stocks on the expiration day. If many arbitragers execute large buy or sell orders on the expiration day, abnormal trading volumes are likely to be observed. If a lot of arbitragers unwind positions in the same direction, temporary trading imbalances induce abnormal stock market volatility. By contrast, if some information arrives at market, the abnormal trading activity must be considered a normal process of price discovery. Stoll and Whaley(1987) investigated the aggregate price and volume effects of the S&P 500 index on the expiration day. In a related study, Stoll and Whaley(1990) found a similarity between the price behavior of stocks that are subject to program trading and of the stocks that are not. Thus far, there have been few studies about the expiration-day effects in the Korean stock market. While previous Korean studies use the KOSPI 200 index data, we analyze the price and trading volume behavior of individual stocks as well as the index. Analyzing individual stocks is important for two reasons. First, stock index is a market average. Consequently, it cannot reflect the behavior of many individual stocks. For example, if the expiration-day effects are mainly related to a specific group, it cannot be said that the expiration of derivatives itself destabilizes the stock market. Analyzing individual stocks enables us to investigate the scope of the expiration-day effects. Second, we can find the relationship between the firm characteristics and the expiration-day effects. For example, if the expiration-day effects exist in large stocks not belonging to the KOSPI 200 index, program trading may not be related to the expiration-day effects. The examination of individual stocks has led us to the cause of the expiration-day effects. Using the intraday data during the period May 3, 1996 through December 30, 2003, we first examine the price and volume effects of the KOSPI 200 and NON-KOSPI 200 index following the Stoll and Whaley(1987) methodology. We calculate the NON-KOSPI 200 index by using the returns and market capitalization of the KOSPI and KOSPI 200 index. In individual stocks, we divide KOSPI 200 stocks by size into three groups and match NON-KOSPI 200 stocks with KOSPI 200 stocks having the closest firm characteristics. We compare KOSPI 200 stocks with NON-KOSPI 200 stocks. To test whether the expiration-day effects are related to order imbalances or new information, we check price reversals on the next day. Finally, we perform a cross-sectional regression analysis to elaborate on the impact of the firm characteristics on price reversals. The main results seem to support the expiration-day effects, especially on stock index futures expiration days. The price behavior of stocks that are subject to program trading is shown to have price effects, abnormal return volatility, and large volumes during the last half hour of trading on the expiration day. Return reversals are also found in the KOSPI 200 index and stocks. However, there is no evidence of abnormal trading volume, or price reversals in the NON-KOSPI 200 index and stocks. The expiration-day effects are proportional to the size of stocks and the nearness to the settlement time. Since program trading is often said to be concentrated in high capitalization stocks, these results imply that the expiration-day effects seem to be associated with program trading and the settlement price determination procedure. In summary, the expiration-day effects in the Korean stock market do not exist in all stocks, but in large capitalization stocks belonging to the KOSPI 200 index. Additionally, the expiration-day effects in the Korean stock market are generally due, not to information, but to trading imbalances.

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The Output Factor of Small Field in Multileaf Collimator of 6 MV Photon Beams (다엽제한기 소조사면의 6 MV 광자선 출력선량계수)

  • Lee, Ho Joon;Choi, Tae-Jin;Oh, Young Kee;Jeun, Kyung Soo;Lee, Yong Hee;Kim, Jin Hee;Kim, Ok Bae;Oh, Se An;Kim, Sung Kyu;Ye, Ji Woon
    • Progress in Medical Physics
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    • v.25 no.1
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    • pp.15-22
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    • 2014
  • The IMRT is proper implement to get high dose deliver to tumor as its shape and selective approach in radiation therapy. Since the IMRT is performed as modulated the radiation fluence by the MLC created the open shapes and its irradiation time, the dose of segment of radiation field effects on the cumulated portal dose. The accurate output factor of small and step shape of segment is important to improve the determination of deliver tumor dose as it is directly proportional to dose. This experiment performed with the 6 MV photon beam of Clinac Ex(Varian) from $3{\times}3cm^2$ to $0.5{\times}0.5cm^2$ small field size for collimator jaw in MLC free and/or for MLC open field in fixed collimator jaw $10{\times}10cm^2$ using the CC01 ion chamber, SFD diode, diamond detector and X-Omat film dosimetry. As results of normalized to the reference field of $10{\times}10cm^2$ of MLC, the output factor of $3{\times}3cm^2$ showed $0.899{\pm}0.0106$, $0.855{\pm}0.0106$ for $2{\times}2cm^2$, $0.764{\pm}0.0082$ for $1{\times}1cm^2$ and $0.602{\pm}0.0399$ for $0.5{\times}0.5cm^2$. The output factor of MLC open field has shown a maximum 3.8% higher than that of the collimator jaw open field.

Studies on the Isolation of Lysozyme from the Egg White and the Determination of It's Bacteriolytic Activities (난백(卵白) Lysozyme의 분리법(分離法) 및 용균활성(溶菌活性)의 측정(測定)에 관(關)한 연구(硏究))

  • Her, Yong;Oh, Hong Rock
    • Korean Journal of Agricultural Science
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    • v.14 no.2
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    • pp.272-285
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    • 1987
  • In order to obtain the data for the effective separation and purification of lysozyme from egg white, optimal conditions for the homogenization of egg white and lysozyme activities of some fresh hen eggs were examined. The recovery and purity of lysozyme isolated by the direct crystallization method, the adsorption with Bentonite and the batch method with Duolite were also investigated. The results obtained were summarized as follows; 1. On the homogenization of egg white, optimal stirring time and stirring rate for the measurement of the lysozyme activities were found to be 10 min. and 2,000 rpm respectively. 2. On the activities of lysozyme in the fresh hen eggs, the activities of W. Leghorn was greater than that of R. Island or Ogol fowl, and the activities of the thick white was a little greater than that of the thin white. 3. The residual lysozyme activities of the hen eggs stored at $4^{\circ}C$ was greater than that of the ones stored at $25^{\circ}C$ or $-20^{\circ}C$ over the storage periods. 4. Hen egg lysozyme recrystallized three times by the method of direct crystallization showed the recovery of 64.3% and the increase of 29 fold in specific activities. 5. By the adsorption method with bentonite, lysozyme in the egg white was adsorbed to 95.7%, and the elution rate of the ones adsorbed was 89.1%, and the increase in specific activities was 13 fold. 6. In the experiment exploying duolite as an adsorbent, Jysozyme in the egg white was obtained with the bound rate of 97%, the recovery rate of 84.8%, and was purified by 30fold.

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