• Biomedical Science Letters
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• v.13 no.3
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• pp.207-212
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• 2007

The purpose of this preliminary study is to improve the efficiency of gene transfer of nonviral plasmid DNA by in vivo electroporation in ischemic hindlimb muscle, tibialis anterior. Hindlimb ischemic model was aseptically made by excision of left femoral artery. Each $50\;{\mu}g$ of pEGFP-C1 and pGL3-control in $100\;{\mu}l$ 0.9% NaCl was injected in tibialis anterior muscle. In vivo electroporation was applied on the same site with 10 mm-distance 2 needle array electrodes and ECM830. In 3 groups of normal rat with different electric field strength 0, 200 and 800 V/cm, the expression of pEGFP-C1 was comparatively evaluated. In 8 groups of normal rats, the expression of pGL3-control was evaluated in 0, 40, 50, 80, 100, 150, 200 and 300 V/cm of electric field strength. In 5 groups of ischemic models, the expression of pGL3-control was analyzed on 0, 4, 7, 10 and 14 days elapsed after making ischemic models. In 9 groups of ischemic rats, the expression of pGL3-control was analyzed in the electric field strength 0, 60, 70, 80, 100, 150, 200, 250 and 300 V/cm. GFP expressions in normal tibialis anterior were high in the extent and degree in order of electric field strength of 200, 800 and 0 V/cm. Luciferase value was highest in $50{\sim}100\;V/cm$ electric field strength. In the case of ischemic models, luciferase expression was significantly increasing in the order elapsed time after making the model. The degree of luciferase expression was higher in cases of application of in vivo electroporation than in that of non-application and was highest in $100{\sim}150\;V/cm$. In conclusion, in vivo electroporation is effective in transfer and expression of plasmid DNA in normal and ischemic tibialis anterior of rat.

• Journal of Korean Physical Therapy Science
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• v.29 no.2
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• pp.28-37
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• 2022

Background: The purpose of this study was to investigate the effects of differences in the tension of Kinesio tape applied to the tibialis anterior muscle on muscle strength, joint range of motion and balance of ankle joint in young adults. Design: Randomized Controlled Trial. Methods: 41 young college students participated in this study. And the subjects were allocated randomly to two group. The experimental group had a tension difference (10%G, n=20). And there was no difference in tension in the control group (0%G, n=21). Jtech power track II, goniometer, and Biorescue were used to measure muscle strength, joint range of motion and balance. The paired t-test was performed to examine the differences within the groups before and after taping, and the independent t-test was performed to examine the differences between the groups. Result: 1) As a result of measuring muscle strength of each group, all measurements except 0%G of plantar flexion showed insignificant results. 2) Measurement of joint range of motion each group showed that neither 0%G nor 10%G was significant. 3) As a result of measuring the baladncing ability of each group there was a significant decrease in length, and average speed of the balancing capability (p<.05). 4) Comparisons between groups showed significant differences in the area among balance capability (p<.05). Conclusions: Based on these findings, the tension difference of Kinesio tape applied to the tibialis anterior muscle shows little effect on the muscle strength, range of motion, and balance of ankle joints. Given the current lack of research on the tension of kinesio tapes many studies are needed in the future to establish clear theories.

• Journal of the Korean Society of Physical Medicine
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• v.6 no.2
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• pp.215-223
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• 2011

Purpose: The purpose of this study was to determine the effect of leg muscle activation by applying proprioceptive neuromuscular facilitation (PNF) arm patterns to unilateral upper extremities under the condition of both open and closed kinetic chains in a seated position. Methods: Twenty-two healthy subjects participated in this study. Four PNF patterns were applied to each subject's unilateral upper extremity. EMG data were collected from the vastus medialis, biceps femoris, tibialis anterior and gastrocnemius. The measured EMG data were digitized and processed to root mean square (RMS) and expressed as percentage maximal voluntary isometric contraction (%MVIC). The data were analyzed using two-way analysis of variance (ANOVA) with repeated measures to determine the statistical significance. Results: The results of this study were summarized as follows: Firstly, in comparison to muscle activation of the biceps femoris, there was a significant increase in the D2 flexion pattern when it was compared with D2 extension pattern and when it was compared with D1 flexion pattern (p<.05). Secondly, there was a significant increase in the muscle activation of the vastus medialis and tibialis anterior with a closed kinetic chain rather than an open kinetic chain (p<.05). Conclusion: In conclusion, in order to increase muscle activation of the biceps femoris, the D2 flexion pattern can be applied, regardless of kinetic chain. In addition, in order to increase muscle activation of the vastus medialis and tibialis anterior, four arm patterns can be applied with a closed kinetic chain.

• The Journal of Korean Physical Therapy
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• v.34 no.1
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• pp.12-17
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• 2022

Purpose: Ankle dorsiflexion is an essential element of normal functions, including walking, activities of daily living and sport activities. The tibialis anterior (TA) muscle functioned as a dorsiflexor and as a dynamic stabilizer of the ankle joint during walking and jumping. This study aimed to compare TA muscle thickness using ultrasonography according to the four different toe and ankle postures for the selective TA strengthening exercise. Methods: This study were recruited 26 (males: 15, females: 11) aged 20-30 years, with no injury ankle and calf in the medical history, had normal dorsiflexion and inversion range of motion (ROM). The thickness of the TA muscle was measured by ultrasonography in the four different toe and ankle postures: 1. Ankle dorsiflexion with all toe extension and ankle inversion (ITEDF); 2. Ankle dorsiflexion with all toe flexion and ankle inversion (ITFDF); 3. Ankle dorsiflexion with all toe extension and neutral position (NTEDF); 4. Ankle dorsiflexion with all toe flexion and neutral position (NTFDF). One-way repeated analysis of variance (ANOVA) and Bonferroni correction were used to confirm the significant difference among conditions. The level of statistical significance was set at α=0.01. Results: TA muscle thickness with ITFDF was significantly greater than in any other ankle positions, including ITEDF, NTFDF, and NTEDF (p<0.01). Conclusion: Among the four toe and ankle postures, isometric contraction in ITFDF postures showed the greatest increase in thickness of TA rather than ITEDF, NTEDF, and NTFDF postures. Based on these results, ITFDF can be recommended in an efficient way to selectively strengthen TA muscle.

• PNF and Movement
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• v.20 no.1
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• pp.115-123
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• 2022

Purpose: The aim of this study was to investigate the effects of sit-to-stand training with various foot positions combined with visual feedback on muscle onset time and balance in stroke patients. Methods: Thirty stroke patients were randomly assigned into three standing groups: one with a symmetrical foot position (SSF; n = 10), one with an asymmetrical foot position with the affected foot at the rear (SAF; n = 10), and one with visual feedback and an asymmetrical foot position (SVAF; n = 10). Sit-to-stand training with different foot positions was performed for 30 minutes a day, 5 times a week, for a total of 4 weeks. The effects on muscle onset time and balance were assessed. Results: In a comparison of the onset time of muscle contraction, the onset time of the affected side tibialis anterior and less-affected side gastrocnemius muscle and tibialis anterior was significantly shortened in the SAVF group. And onset time of the less-affected side tibialis anterior was shortened in the SAF group. There was a significant difference in the result of functional reach testing in the SVAF group. Conclusion: VRG was effective in improving muscle activity and balance in elderly women aged 65 and older. In this study, sit-to-stand training with visual feedback and asymmetrical foot position showed significant functional improvement.

• Journal of Korean Physical Therapy Science
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• v.16 no.2
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• pp.39-44
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• 2009

Background: The purpose of this study was to investigate the comparison between the walking speed and muscle tone of the lower extremity in male and female. Method: The experimental group was that 22 female and 24 male that participated this study. Subjects were measured walking speed and muscle tone. Muscle tone measured using myotonometer. Measured muscle were rectus femoris, vastus medialis, vastus lateralis, biceps femoris, semitendinousus, tibialis anterior, gastrocnemius. Result: The result of this study were that the walking speed were significantly significant(p<.01) in male and female. Muscle tone different in male and female that vastus medialis, vastus lateralis, tibialis anterior and gastrocnemius significantly significant(p<.05). Conclusion: This study showed that difference muscle tone and walking speed in male and female. This indicates that there is an interaction that plays a crucial roles in the walking speed and muscle tone.

• Journal of The Korean Society of Integrative Medicine
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• v.6 no.2
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• pp.117-123
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• 2018

Purpose : The purpose of this study was to investigate the effect of increased brain activity on the muscle endurance and the effect of brain activation through the combination of transcranial direct current stimulation and aerobic exercise on elderly woman. Methods : To investigate the effect of the muscle endurance on right leg, muscle endurance was evaluated by measuring the surface EMG of the muscles of the rectus femoris, biceps femoris, tibialis anterior, and gastrocnemius muscle. Results : There was a significant difference in the pre and post comparisons of muscle endurance on rectus femoris, biceps femoris, tibialis anterior muscle (p<.05). Difference of Combination of transcranial direct current stimulation and aerobic exercise group median frequency was smaller than control group (p<.05). There was not a significant difference in the pre and post comparisons of muscle endurance on biceps femoris, and gastrocnemius muscle. Difference of Combination of transcranial direct current stimulation and aerobic exercise group median frequency was showed a similar pattern. Conclusion : Through these results, It was found that increasing the brain activity by the transcranial direct current stimulation improves the exercise capacity on the elderly women. Combination of transcranial direct current stimulation and aerobic exercise maybe applied as an effective treatment for improving muscular endurance.

• PNF and Movement
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• v.12 no.3
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• pp.133-142
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• 2014

Purpose: The purpose of this study was to investigate the effect of balance using ankle strategy during adjusted balance training and muscle training in normal young female adults. Methods: There were eighteen participants. All subjects were randomly assigned to three groups, the control group (n=6), the adjusted balance training group (n=6) and the muscle training group (n=6). Subjects were assessed for each subject by a pretest and a posttest at 1 week, 2 weeks, and 3 weeks, measuring the anterior-posterior limit of stability, trace length and EMG data for the tibialis anterior and lateral gastrocnemius. Results: The anterior-posterior limit of stability significantly increased in the training group (p<0.05). The trace length significantly decreased in the training group (p<0.05). The muscle activity of the tibialis anterior and lateral gastrocnemius also significantly decreased in the training group (p<0.05). Conclusion: We confirmed that both the balance training group and the muscle training group significantly increased the anterior-posterior limit of stability and decreased trace length and muscle activity. Further studies are needed to analyze the long-term effects on subjects resulting from these changes.

• Korean Journal of Applied Biomechanics
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• v.17 no.2
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• pp.131-143
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• 2007

In this study, we are concluded like this : 5 men who are soccer player of P university in B city measure the Electromyography with an angle of motion according to kinesiotaping's application when practicing in-step kick. When exercising in-step kick, the change of realization of muscle load of lower limbs enhence Gastrocnemius Lateralis and Vastus Medialis in the section of backswing, and improve Tibialis Anterior, Vastus Medialis, Rectus Femoris prior to impact after back-swing. Before impact, it mainly impoved Tibialis Anterior, Vastus Medialis, Rectus Femoris. After impact, it generally improved Gastrocnemius Lateralis, Vastus Medialis. Average integral electromyography value, it was such a small difference(; the difference of the value in Tibialis Anterior, Rectus Femoris, Vastus Medialis) that we can't compare case of after taping than before. In Electromyography, in case of after taping was considerably decreased at Gastrocnemius Lateralis, there was statistically significant difference between before and after. It was a little increased, after taping than before at Knee angle. And degree was a little decreased at Ankle angle. But, It's so delicate difference, there was not statistically significant difference between before and after.

• Korean Journal of Applied Biomechanics
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• v.20 no.3
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• pp.327-336
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• 2010

The purpose of this study was to analyze muscle activities on functional insole with diet effect. Originally, ten healthy female subjects with an average age of 23.2 year(S.D=1.1), weight of 49.7 kg(S.D=4.9), height of 163.2 cm(S.D=3.5) and a shoe size of 237.5 cm(S.D=4.9) were participated in this experiment. Ten healthy females walked on a treadmill(speed=about 4.2 km/h) wearing two different insole types. Muscle activities data was collected using the EMG operating system. The surface EMG signal for tibialis anterior(TA), gastrocnemius(GA), vatus lateralis(VL) and biceps femoris(BF) were acquired at the RMS(10 Hz, 350 Hz) using Noraxon Telemyo DTS system(Noraxon inc, USA). This study processed the data using the Windows SPSS ver.17.0 to get an independent t-test, with the setting, p<.05. Analysis of muscle activity were measured and calculated during walking. The results are as follow: Functional insole wearing were increased muscle activities significantly from Tibialis anterior(TA) during total gait cycle. Normal distribution was demonstrated in total step of stances period. One foot standing position showed decreased muscle activity. Two foot standing position was demonstrated with gastrocnemius and biceps femoris. As a result of the analysis, Functional insole will inerease the diet effect in the use of four muscle groups.