• Journal of Korean Society of Forest Science
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• v.78 no.1
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• pp.30-34
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• 1989

Effects on noise attenuation of the hedge species, Thuja orientalis L. and Euonymus japonicus Thunb., widely used as road side hedges, were analysed at the frequencies of 1,000, 2,000 and 3,150 Hz., respectively. The present experiments were carried out both in the field and in a Semianechoic Chamber. As increasing the distances from the noise source, attenuation rates of noise level were increased at high frequencies. But the attenuation rates of the noise level at low frequencies showed varied as the distances increased, and this trend appeared to be caused by the influence of ground waves. Generally, in this experiment, Thuja orientalis L., which is a coniferous species, showed more effective on the abilities of noise attenuation than that of Euonymus japonicus Thunb., which is a broad-leaved evergreen species.

• Journal of Life Science
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• v.21 no.5
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• pp.746-752
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• 2011

The contents of bioactive materials (e.g. polyphenolics compounds, flavonoids, minerals, and fatty acids) and antioxidative activities (DPPH (${\alpha}$,${\alpha}$'-diphenyl-${\beta}$-picrylhydrazyl) free radical scavenging activity, peroxidation of linoleic acid and rat hepatocyte microsome, and Fe/Cu reducing power) were tested by in vitro experimental models using water, ethanol and methanol extracts of leaves (TOL) and fruits (TOF) from Thuja orientalis. Methanol extract from TOL showed the highest extraction yield (12.90%) as well as contents of polyphenolic compounds (16.02%) and flavonoids (0.25%). Major minerals were Ca, K, and Mg. Major fatty acids were palmitic and lauric acids in TOL and palmitic and decanoic acids in TOF. In oxidation of in vitro models using DPPH free radical scavenging activity, Fe/Cu reducing power, $Fe^{2+}$/ascorbate-induced linolenic acid peroxidation by ferric thiocyanate and thiobarbituric acid (TBA) methods, and autooxidation of rat hepatic microsomes membrane, anti-oxidative activities were stronger in all extracts of TOL than in those of TOF in a dose-dependent manner. From these results, methanol extract of TOL was shown to have the most potent anti-oxidative properties and the highes content of antioxidative compounds such as polyphenolic compounds and flavonoids.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.32 no.2
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• pp.116-123
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• 2014

The description of vegetation cover and floral composition was undertaken in terms of phytosociological study in Dodamsambong(scenic site no. 44) and Danyangseokmoon(no. 45). In this study a total of 17 $relev{\acute{e}}s$ containing 144 taxa were collected and analyzed. Eight plant communities are differentiated, grouped into 4 physiognomic types: forest type(Buxus microphylla var. koreana-Thuja orientalis community, Tilia mandshurica-Quercus variabilis community, and Cynanchum wilfordii-Pinus densiflora community), mantle type(Cardamine leucantha-Neillia uekii community), secondary meadow type(Galium kinuta-Spodiopogon sibiricus community, Diarthron linifolium-Zoysia japonica community), and crevice type(Patrinia rupestris-Selaginella stauntoniana community, Hypodematium glandulosopilosum community). The vegetation of Dodamsambong and Danyangseokmoon is characterized by local flora, such as calciphilous plants, geological distribution-limit species, and endemic species. The soil depth, slope, and human impact have been identified as the most important differentiating ecological factors. Buxus microphylla var. koreana-Thuja orientalis community, Tilia mandshurica-Quercus variabilis community, and Patrinia rupestris-Selaginella stauntoniana community were evaluated highly by National Vegetation Naturalness. In order to restore the value of specific landscape for scenic site, we should improve the problems of protected area such as wrong management on habitat, forest fragmentation by facilities and decline in vegetation by lack of growing the next succession.

• Korean Journal of Medicinal Crop Science
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• v.11 no.5
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• pp.364-370
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• 2003

Essential oil of Thuja occidental leaves was extracted by steam distillation method, and chemical composition of essential oil was analyzed by GC-MS. The essential oil was tested to antimicrorbiaI activity against 28 test microorganisms and antioxident assay through DPPH free radical scaveging test and insecticidal activity by mosquito insecticide assay. The major constituents in the essential oil of T. occidental were monoterpene as d-isothujone, ${\alpha}-thujone$, camphor, L-fenchone, and hornyl acetate. The essential oil have broad antimicrobiaI activity, which showed strong antimicrobiaI activity for Streptococcus pyogenes as bacteria, Alternaria alternata, Aspergillus niger, Aspergillus oryzae, Saccharomyces cerevisiae, and Streptococcus mutans as fungi. The essential oil also showed DPPH free radical scavenging ability at concentration of $36\;{\mu}{\ell}/m{\ell}$. In spraying and fumigation tests, the essential oil had strong insecticidal activity against mosquito.

• The Korea Journal of Herbology
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• v.29 no.3
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• pp.27-33
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• 2014

Objectives : The aim of this study was to investigate the protective effect of extract of Thuja orientalis leaves (TOFE) against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity by inhibition of inflammation in in vitro and in vivo models of Parkinson's disease (PD). Methods : We evaluated the effect of TOFE against lipopolysaccharide (LPS)/1-methyl-4-phenylpyridinium ($MPP^+$) toxicity using nitric oxide (NO) assay, inducible NO synthase and cyclooxygenase 2 western blot, tyrosine hydroxylase and microglia activation immunohistochemistry (IHC) in BV2 cell, primary rat mesencephalic neurons, or C57BL/6 mice. We also evaluated the effect of TOFE in mice PD model induced by MPTP. C57BL/6 mice were treated with TOFE 50 mg/kg for 5 days and were injected intraperitoneally with four administrations of MPTP on the last day. We conducted behavioral tests and IHC analysis to see how TOFE affect MPTP-induced neuronal loss of dopaminergic neurons in substantia nigra pars compacta (SNpc) and striatum (ST) of mice. To assess the anti-inflammation effects, we carried out glial fibrillary acidic protein and macrophage-1 antigen integrin alpha M in IHC in SNpc and ST of mice. Results : In an in vitro system, TOFE decreasesd NO generations in BV2 cells. TOFE protected dopaminergic cells against LPS or $MPP^+$-induced toxicity in primary mesencephalic dopaminergic neurons. In vivo system, TOFE at 50 mg/kg treated group showed improved motor deteriorations than the MPTP only treated group and TOFE significantly protected striatal dopaminergic damage from MPTP-induced neurotoxicity in mice. Moreover, TOFE inhibited activation of astrocyte and microglia in SNpc and ST of the mice. Conclusions : We concluded that TOFE showed anti-parkinsonian effect by protection of dopaminergic neurons against MPTP toxicity through anti-inflammatory actions.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.179-179
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• 2003

• 보존과학연구
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• s.25
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• pp.197-226
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• 2004

This study was made to identify species of archaeological wood products excavated from three sites(Kwunbuk-ni, Gungnamgi, Wanggung-ni) of Sabi Era, Baekje. These sitesare presumably considered as capital areas of Sabi Era, Baekje. Total of 220 specimens were identified into five softwoods and eighteen hardwoods. Pinus densiflora(or Pinusthunbergii) and Quercus spp. are the most common and occupied 32%, 28% of totalspecimen, respectively. The others are follows : Torreya nucifera(1%), Abiesholophylla(3%), Cryptomeria japonica(4%), Thuja spp.(2%), Chamaecyparisobutusa(2%), Salix spp.(1%), Platycarya strobilacea (3%), Alnus spp.(1%), Carpinusspp.(0.5%), Castanea crenata(9%), Zelkova serrata (6%), Celtis spp.(0.5%), Prunusspp.(2%), Rhus verniciflua(1%), Rhus trichocarpa(0.5%), Meliosma oldhamii(1%),Hovenia dulcis(1%), Kalopanax pictus(0.5%), Cornus walteri(0.5%), Styrax japonica(1%),Fraxinus rhynchophylla (0.5%), F. sieboldiana(1%).Most of the identified species have been growing in this area until nowadays. However,a few species(Cryptomeria japonica, Thuja spp. Chamaecyparis obutusa, Torreyanucifera) didn’t grow natively around this area at that time. Two species(Cryptomeriajaponica, Chamaecyparis obutusa) are endemic species of Japan, indicating international trade or exchange of woods between Baekje Kingdom and Japan in 6-7th century. Torreyanucifera grows in limited areas in Korea (south of $35^{\circ}$10′N), however, is widely distributed in the southern Japan. Quercus spp. was identified the most in implements of Baekje and Pinus densiflora(orPinus thunbergii) was in the second place. Zelkova serrata was mainly used for raw materials of turnery products, e.g., wooden container. This species produces one of the hardest woods as 0.7 in specific gravity. It indicates that iron technologies have reached some high level in Sabi Era, Beakje.

• Journal of Korean Society for Atmospheric Environment
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• v.7 no.3
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• pp.208-218
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• 1991

To compare the resistance of landscape trees by acid rain, simulated acid rain was treated on the seeds of 6 coniferous species (Pinus densiflora, Pinus thunbergii, Pinus koraiensis, Taxus cuspidata, Cedrus deodara, Thuja occidentalis) and 6 broad-leafed species (Ligustrum obtusifolium, Acer Buergerianum, Acer Ginnala, Styrax obassia, Cornus Kousa, Magnolia obovata). The experimental design of randomized block arrangement with three replications was implemented in the experimental field of Yesan National Agri-Tech Junior College. The seeds of 12 tree species were planted on the nursery soil in the early spring of 1991. The regime of artificial acid rain, in terms of spray frequency per monthly and spray amount at year records. Simulated acid rain (pH 2.0, pH 4.0, pH 5.6 as control) containing sulfuric and nitric acid in the ratio of 3:2 (chemical equivalant basis) diluted ground water, were treated on the experimental plants under condition of cutting off the natural precipitation with vinyl tunnel, during the growing season (April 8 to August 31) in 1991. Seedling establishment, seedling growth and nutrient contents in needles and leaves were measured and compared among the treatment. The results were summerized as follows; 1. In general, coniferous species were more resistant than broad-leafed species in the resistant comparisons of landscape trees by simulated acid rain. But there were resistant tree species to acid rain among the broad-leafed species. 2. Among 6 coniferous species used in this experiment, Pinus densiflora, Pinus koraiensis, Taxus cuspidata, Cedrus deodara and Pinus thunbergii revealed the resistant tree species to acid rain. But Thuja occidentalis was very susceptible to acid rain, because of no germination in pH 2.0 treatment plot of acid rain. 3. Among 6 broad-leafed species used in this study, Magnolia obovata, Styrax obassia and Ligustrum obtusifolium showed relative resistant tree species to acid rain. But Acer Buergerianum, Acer Ginnala and Cornus Kousa were very susceptible to acid rain, because of no germination in pH 2.0 plot of acid rain treatment.

• Journal of Aquaculture
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• v.14 no.2
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• pp.81-87
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• 2001

Antimicrobial activity of the essential oil extracted from plants Artemisia princeps var. orientalis, Thuja orientalis and Chamaecyparis obtusa were tested against selected pathogenic bacteria and fungi of fish. At the concentrations above 500ppm, ingibitory effect of the oil of A. princeps var. orientalis was at its peak against Aeromonas hydrophila ATCC 14715, A. hydrophila CF-2, A. salmonicida ATCC 14174 and A. salmonicida EL-1 but the bacteria Edwardsiella tarda KBF-1, Vibrio anguillarum ATCC19264, V. ordalii ATCC33509 and Streptococcus sp. were insensitive. Likewise, the oil extract of T. orientalis showed the highest inhibitory activity against V. ordalii ATCC33509, E. tarda ECK-1, and E. tarda KBF-1 at 300ppm; however the activity was highest at 500ppm or A. hydrophila ATCC14715, A. hydrophila CF-2, A. salomonicida ATCC14174, A. salmonicida EL-1 and Streptococcus sp. SF-1. With increasing dose of C. obtusa oil, the inhibitory activity became more and more effective against A. hydrophila CF-2, A. salomonicida ATCC14174, E. tarda ECK-1 and Streptococcus sp. SF-1, but A. hydrophila ATCC14174, A. salmonicida EL-1, E. tarda KBF-1, V. anguillarum ATCC19264, V. ardalii ATCC33509 and gram positive bacteria (Streptococcus sp.) were somewhat resistant. A. princeps var. orientalis, T. orientalis and C. obtusa were also tested against Saprolegnia sp. at the oil concentrations of 10, 100, 500, 1,000, 1,500 and 2,000ppm. The inhibitory effect of the oil on the inhibit the mycelial growth of Saprolegnia sp. at 10ppm and completely inhibited at over 500ppm.

• Journal of Microbiology and Biotechnology
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• v.27 no.7
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• pp.1242-1248
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• 2017

Several medicinal plants are ethnomedically used in Korea as agents for treating infection, anti-inflammation, and pain relief. However, beyond typical inhibitory effects on cell growth, little is known about the potential anti-biofilm activity of these herbs, which may help to prevent cavities and maintain good oral health. This study aimed to investigate the antimicrobial and anti-biofilm activities of the methanol extracts of 37 Korean medicinal plants against dental pathogens Streptococcus mutans and Candida albicans, which synergize their virulence so as to induce the formation of plaque biofilms in the oral cavity. The antimicrobial activities were investigated by broth dilution and disk diffusion assay. The anti-biofilm and antioxidant activities were evaluated based on the inhibitory effect against glucosyltransferase (GTase) and the DPPH assay, respectively. Among 37 herbs, eight plant extracts presented growth and biofilm inhibitory activities against both etiologic bacteria. Among them, the methanol extracts (1.0 mg/ml) from Camellia japonica and Thuja orientalis significantly inhibited the growth of both bacteria by over 76% and over 83% in liquid media, respectively. Minimum inhibitory concentration (MIC) values of these methanol extracts were determined to be 0.5 mg/ml using a disk diffusion assay on solid agar media. Biofilm formation was inhibited by more than 92.4% and 98.0%, respectively, using the same concentration of each extract. The present results demonstrate that the medicinal plants C. japonica and T. orientalis are potentially useful as antimicrobial and anti-biofilm agents in preventing dental diseases.