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Rapid detection of the hepatitis a virus from fresh lettuce using immunomagnetic separation and quantum dots assay (IMS-QD assay를 활용한 상추에서 간염 A형 바이러스의 신속순수분리 및 형광 검출법 연구)

  • Lee, Hee-Min;Kwon, Joseph;Choi, Jong-Soon;Won, Yong-Gwan;Kim, Eun-Sun;Chung, Jae-Keun;Kim, Min-Ji;Kim, Duwoon
    • Food Science and Preservation
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    • v.21 no.2
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    • pp.170-174
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    • 2014
  • Hepatitis A virus (HAV) infection leads to acute liver failure and death through the intake of contaminated food. The polymerase chain reaction (PCR) has been used to detect HAV in food samples. HAV detection takes a long time, however, due to the virus concentration step required before PCR assay. In this study, a rapid method of detecting the HAVs present in lettuce using immunomagnetic separation combined with quantum dots (IMS-QDs) assay was developed. The detection limit of IMS-QDs for HAV was 10 $TCID_{50}/mL$, similar to the result that was obtained using RT-PCR combined with PEG or IMS. The application of IMS-QDs assay completed the viral detection within one hour, but this was not possible using PEG combined with RT-PCR. In conclusion, IMS-QDs assay is a rapid and efficient method for detecting HAV at a low concentration in agricultural products.

A 3-D finite element analysis on the mandibular movement pattern and stress distribution during symphyseal widening (하악 이부확장 시 하악골 이동 양상과 응력 분포에 관한 삼차원 유한요소법적 연구)

  • Lee, Do-Hoon;Hong, Hyun-Sil;Chae, Jong-Moon;Jo, Jin-Hyung;Kim, Sang-Cheol
    • The korean journal of orthodontics
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    • v.38 no.1
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    • pp.13-30
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    • 2008
  • Objective: The objective of this study was to evaluate the displacement pattern and the stress distribution of the finite element model 3-D visualization during symphyseal widening according to the osteotomy position, osteotomy type, and distraction device. Methods: The kinds of distraction devices used were tooth-borne type, hybrid type, bone-borne type and tooth-borne type $30^{\circ}$ angulated, and the kinds of osteotomy design were vertical osteotomy line between the central incisors and step osteotomy line through the symphysis. Results: All reference points of the mandible including the condyles were displaced laterally irrespective of the osteotomy position, osteotomy method and distraction device. The anteroposterior or vertical displacements showed small differences between the groups. The widening pattern of the osteotomy line in the tooth-borne type of device was v shaped, and that of bone-borne type was a reverse v shape. However, the pattern in the hybrid type was parallel. The lateral displacement of the mandibular angle by the bone-borne device was more remarkable than the other types of devices. The displacement by the $30^{\circ}$ angulated tooth-borne type was different between the left and right sides in both the transverse and anteroposterior aspects. Conclusion: The design of the distraction devices and osteotomy line can influence the displacement pattern and the stress distribution during mandibular symphyseal distraction osteogenesis procedures.

Expression Pattern of EphB2 in Gastric Cancer (위암에시 EphB2 단백의 발현 양상)

  • Song, Jae-Hwi;Kim, Chang-Jae;Cho, Young-Gu;Park, Cho-Hyun;Nam, Suk-Woo;Yoo, Nam-Jin;Lee, Jung-Young;Park, Won-Sang
    • Journal of Gastric Cancer
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    • v.6 no.1
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    • pp.25-30
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    • 2006
  • Purpose: The EphB2 receptor, a member of the receptor tyrosine kinase family, is a target gene of the Wnt signaling pathway and may achieve a tumor suppressor function through regulation of cell growth and migration. Our aim was to determine whether an altered expression of EphB2 might be associated with gastric cancer development and, if so, to determine to which pathologic parameter it is linked. Materials and Methods: For the construction of the gastric cancer tissue microarray, 83 paraffin-embedded tissues containing gastric cancer areas were cored 3 times and transferred to the recipient master block. The expression patterns of EphB2 were examined on tissue microarray slides by using immunohistochemistry and were compared using pathologic parameters, including histological type, depth of invasion, lymph node metastatsis, and peritoneal dissemination. Results: The EphB2 protein was expressed in the normal gastric mucosal epithelium, especially in the bottom of the mucosa. We found loss of EphB2 expression in 30 (36.1%) of the 83 gastric cancer tissues. Statistically, loss of EphB2 expression was more common in gastric cancer with lymph-node metastasis. There was no significant correlation between EphB2 expression and depth of invasion, histologic type, or peritoneal dissemination. Conclusion: Our findings suggest that loss of EphB2 expression may represent a critical step in gastric carcinogenesis.

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Gamma-ray Exposure Rate Monitoring by Energy Spectra of NaI(Tl) Scintillation detectors

  • Lee, Mo Sung
    • Journal of Radiation Protection and Research
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    • v.42 no.3
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    • pp.158-165
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    • 2017
  • Background: Nuclear facilities in South Korea have generally adopted pressurized ion chambers to measure ambient gamma ray exposure rates for monitoring the impact of radiation on the surrounding environment. The rates assessed with pressurized ion chambers do not distinguish between natural and man-made radiation, so a further step is needed to identify the cause of abnormal variation. In contrast, using NaI(Tl) scintillation detectors to detect gamma energy rates can allow an immediate assessment of the cause of variation through an analysis of the energy spectra. Against this backdrop, this study was conducted to propose a more effective way to monitor ambient gamma exposure rates. Materials and Methods: The following methods were used to analyze gamma energy spectra measured from January to November 2016 with NaI detectors installed at the Korea Atomic Energy Research Institute (KAERI) dormitory and Hanbat University. 1) Correlations of the variation of rates measured at the two locations were determined. 2) The dates, intervals, duration, and weather conditions were identified when rates increased by $5nSv{\cdot}h^{-1}$ or more. 3) Differences in the NaI spectra on normal days and days where rates spiked by $5nSv{\cdot}h^{-1}$ or more were studied. 4) An algorithm was derived for automatically calculating the net variation of the rates. Results and Discussion: The rates measured at KAERI and Hanbat University, located 12 kilometers apart, did not show a strong correlation (coefficient of determination = 0.577). Time gaps between spikes in the rates and rainfall were factors that affected the correlation. The weather conditions on days where rates went up by $5nSv{\cdot}h^{-1}$ or more featured rainfall, snowfall, or overcast, as well as an increase in peaks of the gamma rays emitted from the radon decay products of $^{214}Pb$ and $^{214}Bi$ in the spectrum. This study assumed that $^{214}Pb$ and $^{214}Bi$ exist at a radioactive equilibrium, since both have relatively short half-lives of under 30 minutes. Provided that this assumption is true and that the gamma peaks of the 352 keV and 1,764 keV gamma rays emitted from the radionuclides have proportional count rates, no man-made radiation should be present between the two energy levels. This study proved that this assumption was true by demonstrating a linear correlation between the count rates of these two gamma peaks. In conclusion, if the count rates of these two peaks detected in the gamma energy spectrum at a certain time maintain the ratio measured at a normal time, such variation can be confirmed to be caused by natural radiation. Conclusion: This study confirmed that both $^{214}Pb$ and $^{214}Bi$ have relatively short half-lives of under 30 minutes, thereby existing in a radioactive equilibrium in the atmosphere. If the gamma peaks of the 352 keV and 1,764 keV gamma rays emitted from these radionuclides have proportional count rates, no man-made radiation should exist between the two energy levels.

Installation Technology and Behavior of Silty Clay Filled Geotextile Tube (실트질 점토 채움 시 지오텍스타일 튜브의 거동 및 시공 방법에 관한 연구)

  • Shin, Eun-Chul;Oh, Young-In
    • Journal of the Korean Geosynthetics Society
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    • v.1 no.1
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    • pp.13-21
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    • 2002
  • Geotextile tubes hydraulically or mechanically filled with dredged materials have been applied in hydraulic and coastal engineering in recent years(detached breakwater, groins and jetty). The geotextile tubes are made of sewn geosynthetics sheets. If the sandy soil is use to fill material, these inlets should be spaced closely to assure uniform filling of the tubes because sandy soil and geosynthetic is very pervious. However, the clayey soil or contaminated slurry is used, the inlets can be located relatively long distance. The fine clayey particles tend to rapidly blind the fabric slowing down water escape through the geotextile. This paper presents a field test result of a geotextile tube in the land reclamation project for the Songdo New City construction site. The dredged silty clay was dredged by the dredging ship and hydraulically pumped into the geotextile tube. The height of geotextile tube was measured at every filling stage and also measured width and diameter of geotextile tube with the elapsed time. Based on the test results, if the clayey filling material is used, the pumping step must be divided 3~4 stages for drainage and sediment. After complete drainage, the height of the geotextile tube reduces by approximately 50%.

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Macroscopic Analysis of Traffic Flow in the Korean Coastal Waterway (한국연안의 해상교통류분석(I))

  • 이철영;문성혁;최종화;박양기
    • Journal of the Korean Institute of Navigation
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    • v.10 no.2
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    • pp.31-55
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    • 1986
  • Generally, the development of shipping is characterized by the amount of traffic flow (traffic volume) and seaborne cargo in the sea. Movement of ships is an essential element of constructing the traffic flow which is represented the dynamic movement of ships in the sea, but on the other band the numbers of arriving and departing the port is the basic factor consisting of the static movement of ships. The amount of cargoes by coastal vessels and ocean trade vessels have increased tremendously with the great growth of the Korean economy these days. This increase of the seaborne cargoes has made the Korean coastal traffic flow so congested that this can be a cause of large pollution as well as great marine casualities such as a loss of human lives and properties . And also the future coastal traffic is expected to increase considerably according to our economic development and high dependence upon foreign trade. Under the circumstance, to devise the safety of coastal traffic flow and to take a proper step of a efficient navigation, there is a necessity for analyzing and surveying the coastal traffic trend and the characteristics of cargo movement. In order to grasp the dynamic movement of ships in the Korean coast, O/D analysis is executed. This paper aims to secure the basic data necessary for a comprehensive plan and estimation of vessel traffic management system for the enhancement of safety, order and efficiency of vessel traffic in the Korean coast. The analyzed results of the traffic flow and seaborne cargoes of the Korean coast are summarized as follows : 1) The congestion by the vessels occurred around the ports such as-in proportion of ship's number (proportion of tonnage) -Incheon 18.5%(14.8%), Pohang 5.9% (9.9%), Samil 5.2%(8.3%), Mokpo 8.6%(0.8%), Pusan 13.5%(36.4%), Ulsan 9.1%(16.2%). 2) It is found that the area adjacent to Incheon, Pusan, Ulsan, Channel of Hanryu and South-western area are heavily congested. 3) It is confirmed thatthe area adjacent to Incheon, Pusan, Ulsan, Channel of Hanryu and South-western area are heavily congested. 3) It is confirmed that the coastal vessels are main elements constituting the coastal traffic and that there are much traffic flow among five ports as following through the precise O/D analysis of ship's coastal movement. Incheon-Samil, Ulsan, Pusan, Jeju Pusan -Samil, Ulsan, Incheon, Jeju Pohang -Samil, Inchoen, Jeju Pohang -Samil, Incheon, Jeju Ulsan -Samil, Incheon, Jeju Samil -Ulsan, Pusan, Incheon 4) The amount of cargoes to abroad are in proportion about 81% of total and the amount of coastal cargoes are about 19%. Of those, cargoes in and out to Japan are about 26% and to South-east Asia are about 27%. 5) The chief items of foreign cargoes are oil(38.33%), iron ore(13.98%), bituminoous coal(12.74%), grain(8.02%), lumber(6.45%) in the import cargoes and steel material(21.96%), cement(17.16%), oil(6.81%), fertilizer(3.80%) in the export cargoes. 6) The 80.5% of total export cargoes and 92.4% of total import cargoes are flowed in five main ports. 7) The chief items of coastal cargoes are oil (42.45%), cement(16.86%), steel material (6.49%), anthracite(6.31%), mineral product(4.3%), grain, and fertilizer. Almost 92.24% of total import and export oil cargoes in Korea is loaded and unloaded at the port of Samil & Ulsan.

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Study on the Effects of Corrosion Inhibitor According to the Functional Groups for Cu Chemical Mechanical Polishing in Neutral Environment (중성 영역 구리 화학적 기계적 평탄화 공정에서의 작용기에 따른 부식방지제의 영향성 연구)

  • Lee, Sang Won;Kim, Jae Jeong
    • Korean Chemical Engineering Research
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    • v.53 no.4
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    • pp.517-523
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    • 2015
  • As the aluminum (Al) metallization process was replaced with copper (Cu), the damascene process was introduced, which required the planarization step to eliminate over-deposited Cu with Chemical Mechanical Polishing (CMP) process. In this study, the verification of the corrosion inhibitors, one of the Cu CMP slurry components, was conducted to find out the tendency regarding the carboxyl and amino functional group in neutral environment. Through the results of etch rate, removal rate, and chemical ability of corrosion inhibitors based on 1H-1,2,4-triazole as the base-corrosion inhibitor, while the amine functional group presents high Cu etching ability, carboxyl functional group shows lower Cu etching ability than base-corrosion inhibitor which means that it increases passivation effect by making strong passivation layer. It implies that the corrosion inhibitor with amine functional group was proper to apply for 1st Cu CMP slurry owing to the high etch rate and with carboxyl functional group was favorable for the 2nd Cu CMP slurry due to the high Cu removal rate/dissolution rate ratio.

Survey on the Water Quality of Keum River (금강수질(錦江水質)에 관(關)한 조사연구(調査硏究))

  • Park, Jong-Seong;Oh, Man Jin
    • Korean Journal of Agricultural Science
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    • v.6 no.1
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    • pp.65-68
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    • 1979
  • Water of river gets great concern about its quality, which might be polluted with pollutant from various industrial plants booming along the river sides in recent years. As a first step to prevent any applciable damage due to water pollution, survey on the water quality of Keum river at Sintanjin, Maepo, Taepyongri, Kongju, Puyeo and Kanggeng, six regions was conducted during september, 1977 through august, 1978 and the results were summarized as follow: 1. There were no significant regional and monthly difference in pH, $NO_2-N$, and $NO_3-N$ of water. 2. The BOD at Puyeo, Kanggeng was 3.9 to 5.6, 5.6 to 6.0 ppm, respectively, and those values reached the limiting level for tap water sources. 3. The BOD at Maepo, where Daejeon streams meet, was found 1.9, 3.0, and 2.1 times higher than those at Sintanjin region, during september, october and november, respectvely. 4. Content of DO was $7.6{\pm}3.8ppm$ in all surveyed regions and varied with water temperature and regional difference. 5. There were found regional difference and monthly difference in water hardness, suspended solid and $NH_3-N$.

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Production of an Interactive Media Artwork with motif of Characteristics of comets (혜성의 특성을 모티브로 한 인터랙티브 미디어 아트 제작)

  • Lee, O-Jung;Kim, Hyung-Gi
    • Journal of Digital Contents Society
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    • v.18 no.8
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    • pp.1529-1535
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    • 2017
  • Due to its unique formation and mystery phenomena, comets is a major motif that inspires various creative fields. Media art that has been developed and expanded in step with the development of modern scientific technology has expressed the unique characteristics of comets such as their structure in the art as a motif, using technologies. This study describes the process of producing "Near by", an distance-recognition interactive media art. offering the audience a chance to create a tail of a comet as they close to it. changes the color of a LED structure representing the nucleus of a comet as the audience approaches it. At the same time, a video of a comet's tail is projected. Through this interaction, the audience will renew their perception on comets and emotionally interact with the art. This work capturing not only the formative characteristics of a comet, but also its forming process as a motif. Such composite way of expressing a motif could be presented as a possibility of extending the expression of the natural phenomenon in the future media art field.

A TXOP Sharing Scheme for QoS Strategy of IEEE 802.11ac DL MU-MIMO MAC (IEEE 802.11ac DL MU-MIMO MAC의 QoS 정책을 고려한 TXOP 공유 방안)

  • Lee, Ji-Young;Seok, Seung-Joon
    • Journal of Digital Convergence
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    • v.12 no.10
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    • pp.317-327
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    • 2014
  • To improve the efficiency of wireless channel, IEEE 802.11ac uses the DL MU-MIMO MAC scheme through which an AP transmits multiple frames to different mobile nodes simultaneously. IEEE 802.11ac DL MU-MIMO MAC needs a new step, called as TXOP sharing, between legacy IEEE 802.11n DL SU-MIMO's two operations, the obtaining an EDCA TXOP and the transmitting multiple frames for EDCA TXOP. In the TXOP sharing operation, both wireless channel destinations and frames transmitted for its TXOP period should are determined. So this paper deals with the TXOP sharing for improving IEEE 802.11ac MAC performance. However, the EDCA priority based method mentioned by IEEE 802.11ac standard document not fair among the buffers and the frames of buffers, and occurs in high_loss rate and high_delay about specific buffers. In this paper, we propose a new scheme of the TXOP sharing with sequencing p-AC, s-AC in similar properties, and all S-AC. This method provides a differentiated service without damage of EDCA characteristics.