• Clinical and Experimental Reproductive Medicine
• /
• 제49권4호
• /
• pp.259-269
• /
• 2022

Objective: Animal-free scaffolds have emerged as a potential foundation for consistent, chemically defined, and low-cost materials. Because of its good potential for high biocompatibility with reproductive tissues and well-characterized scaffold design, we investigated whether polyglycolic acid (PGA) could be used as an animal-free scaffold instead of natural fibrin-agarose, which has been used successfully for three-dimensional human endometrial cell culture. Methods: Isolated primary endometrial cells was cultured on fibrin-agarose and PGA polymers and evaluated various design parameters, such as scaffold porosity and mean fiber diameter. Cytotoxicity, scanning electron microscopy (SEM), and immunostaining experiments were conducted to examine cell activity on fabricated scaffolds. Results: The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay and SEM results showed that endometrial cells grew and proliferated on both scaffolds. Immunostaining showed cytokeratin and vimentin expression in seeded cells after 7 days of culture. On both scaffolds, an epithelial arrangement of cultured cells was found on the top layer and stromal arrangement matrix on the bottom layer of the scaffolds. Therefore, fibrin-agarose and PGA scaffolds successfully mimicked the human endometrium in a way suitable for in vitro analysis. Conclusion: Both fibrin-agarose and PGA scaffolds could be used to simulate endometrial structures. However, because of environmental and ethical concerns and the low cost of synthetic polymers, we recommend using PGA as a synthetic polymer for scaffolding in research instead of natural biomaterials.

• 한국복식학회:학술대회논문집
• /
• 한국복식학회 2003년도 International Costume Conference
• /
• pp.32-32
• /
• 2003

This research has for its object to look carefully into the peculiarity of the color of the costume in the Korean culture by revealing the characteristic and the idea of the color being discovered in the Korean basic culture, which has the majority of the Korean people. The scope of the basic culture was divided into folk belief, folk game and folk play. Within these limits, the colors of the dress, accessories, instruments were extracted by comparing with the naked eye in NCS Color System. For the analysis of hue and tone the secondary dimensional analysis using NCS color system and the three-dimensional analysis using the software, COLOR 3D Version 2.0, were done.

• 복식
• /
• 제56권5호
• /
• pp.29-43
• /
• 2006

The purpose of this study is to examine a unique characteristic of the colors of the costumes in Korean basic culture in the aim of seeking the characteristics and the conceptual meanings of colors found in the majority’s culture. The scope of the basic culture was divided into folk belief, folk game and folk play. Within these limits, the colors of the dress, accessories, instruments were extracted by comparing with the naked eye in NCS Color System. For the analysis of hue and tone, the secondary dimensional analysis using NCS color system and the three-dimensional analysis using the software, COLOR 3D Version 2.0, were done. The result of this investigation is that the colors of the costume in the Korean basic culture are white, gray and black of achromatic color and yellow, yellowish red and purplish blue. This confirms that the colors based on Five-elements color are becoming the basis too basic culture. And Arche-pattern, which is a characteristic commonly found in the Korean traditional society, was shown as a characteristic of color. The colors of the costumes in Korean basic culture are uniquely adopted by the Korean civilians according to their religious and philosophical living standard. This study is meaningful in seeking a root for the formation of their unique color culture.

• 대한한의학방제학회지
• /
• 제32권2호
• /
• pp.181-191
• /
• 2024

Objective : Inulae Flos(IF) has been used to treat arthritis, sever furuncle, fear and palpitation, vomiting, stroke, asthma and cough in Korean Medicine. Although the anticancer activity of IF has been reported, the molecular mechanism is still not well understood. In this study, we investigated the growth inhibitory activity of an ethanol extract of IF in HT-1080 human fibrosarcoma cells and its underlying mechanisms using two-dimensional (2D) and three-dimensional (3D) cell culture system. Methods : HT-1080 cells were cultured with IF for 9 days in 3D cell culture. To check an inhibition of cell prolifelation by IF, MTT assay was performed. DNA contents were measured using flow cytometry. Western blotting was used to evaluate the regulation of cell cycle- and autophagy-related proteins. Acridine orange staining was performed to confirm autophagy, and DCF-DA staining was performed to confirm the occurrence of ROS. Results : IF controlled a spheroid formation and decreased a cell viability in 3D cell culture. IF-induced cell proliferation inhibition was associated with a distinct increase of S and G2/M phase cell distribution in 2D cell cultre. In addition, IF significantly induced autophagy and generated reactive oxygen species(ROS). Interestingly, IF-induced cell cycle arrest and autophagy were recovered after pre-treatment of N-acetyl-L-cysteine, ROS scavenger. Conclusion : Our results indicate that IF induced ROS-mediated cell cycle arrest and autophagy and it may potentially useful for human fibrosarcoma treatment.

• 스마트미디어저널
• /
• 제3권4호
• /
• pp.22-27
• /
• 2014

3차원 복원은 실세계에 존재하는 물체를 가상의 공간에 재건하고 자유로운 시점을 선택하여 물체를 관찰할 수 있게 한다. 이러한 3차원 복원 기술은 교육, 문화, 예술 등 분야를 막론하고 다양한 곳에서 사용되고 있다. 3차원 복원 시스템을 구현하기 위해 본 논문에서는 Microsoft사에서 출시된 Kinect를 이용하여 다시점 시스템을 구성해서 고품질의 3차원 객체를 복원하는 방법을 제안한다. 먼저 3대의 Kinect를 객체의 전면에 수렴형으로 설치하여 색상 영상과 깊이 영상을 획득한다. 그런데 원본의 깊이 영상은 깊이 값을 가지지 않는 부분이 존재한다. 따라서 본 논문에서는 이 부분을 적절한 깊이 값으로 채워 넣기 위해서 깊이 가중치를 추가한 결합형 양방향 필터를 적용한다. 또한 다시점 시스템에서 얻은 원본의 색상 영상은 서로 색상이 일치하지 않는 문제가 존재하는데 이를 그대로 이용하여 3차원 모델 정합을 하면 색상이 부자연스럽게 연결된 3차원 모델을 얻게 된다. 따라서 이러한 색상 불일치의 문제를 해결하기 위해서 본 논문에서는 다시점 시스템에서의 3차원 기하학적 정보를 이용한 색상 보정 방법을 사용한다. 실험을 통해 제안한 방법을 이용하여 획득한 3차원 모델이 원본 3차원 모델보다 색상과 형태 관점에서 자연스럽게 표현된 것을 확인할 수 있었다.

• Biomolecules & Therapeutics
• /
• 제23권5호
• /
• pp.391-399
• /
• 2015

As a major component of the epidermal tissue, a primary keratinocyte has served as an essential tool not only for the study of pathogenesis of skin-related diseases but also for the assessment of potential toxicities of various chemicals used in cosmetics. However, its short lifespan in ex vivo setting has been a great hurdle for many practical applications. Therefore, a number of immortalization attempts have been made with success to overcome this limitation. In order to understand the immortalization process of a primary keratinocyte, several key biological phenomena governing its lifespan will be reviewed first. Then, various immortalization methods for the establishment of stable keratinocyte cell lines will be explained. Finally, its application to a three-dimensional skin culture system will be described.

• 대한화장품학회지
• /
• 제29권2호
• /
• pp.105-130
• /
• 2003

Human skin equivalents, also designated as cultured skin substitute (Boyce and Warden, 2002) or organotypic co-cultures (Maas-Szabowski et al., 1999, 2000, 2003), are three-dimensional systems that are engineered by seeding fibroblasts into a three-dimensional dermal matrix. Such a dermal equivalent is then subsequently seeded with human keratinocytes. After cell attachment, the culture is kept first under submerged condition to allow keratinocyte proliferation. Thereafter, the culture is lifted the air-liquid interface (A/L) to expose the epidermal compartment to the air, and to further induce keratinocyte differentiation. During the air-exposure, nutrients from the medium will diffuse through the underlying dermal substrate towards the epidermal compartment and support keratinocyte proliferation and differentiation. Under these conditions, a HSE is formed that shows high similarity with the native tissue from which it was derived (Figure 1) (Bell et at., 1981; Boyce et al., 1988; Ponec et al., 1997;El Ghalbzouri et al.., 2002).

• 한국콘텐츠학회:학술대회논문집
• /
• 한국콘텐츠학회 2006년도 추계 종합학술대회 논문집
• /
• pp.836-839
• /
• 2006

애니메이션제작에 있어 과장과 강조를 통하여 코믹한 연출을 만들어 가는 것이 무엇보다도 중요한 과제이다. 본 연구에서는 애니메이션적인 과장과 강조를 Shot에 적용시킨 코믹효과를 연구한다. 먼저 선행단계로 입체적인 Shot을 연출하는 카메라계산법으로 이미지추출 실험과 Shot 이동연출법을 제시한다. 그리고 입체원리로 제작되어진 애니메이션 Shot을 분석하여 코믹효과를 제언한다.

• 한국수정란이식학회지
• /
• 제29권3호
• /
• pp.207-219
• /
• 2014

Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in $2.5{\times}10^5cells/ml$ and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of $IL-1{\beta}$ (0.1, 1, 10 and 100 ng/ml) were higher than without $IL-1{\beta}$, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.

• 수산해양기술연구
• /
• 제44권4호
• /
• pp.353-361
• /
• 2008

Recently a modern style sailing boat suitable for Korean sea was designed through full three dimensional design skill by the authors. In this paper, based on this three dimensional hull and deck design, a production process of 31ft class cruiser sailing boat was developed. First of all, it was possible to make the digital mock-up for design boat. Through this mock-up and RP(rapid prototyping) modeling, an appropriate general arrangement of design boat was able to be determined at final. And also the female deck mould was able to be made by a 5-axis NC cutting machine. By doing this method, more higher efficiency and precision for sailing boat production could be achieved than before. Through this research the total process of design and construction for the designed boat was established.