Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Inulae Flos Induces Cell Cycle Arrest and Autophagy of HT-1080 Human Fibrosarcoma Cells in 2D and 3D Cell Culture Model

선복화의 2D 및 3D 세포배양모델에서 HT-1080 인간 섬유육종 세포의 세포주기정지 및 자가포식 유도 효과

  • Min Young Kim (Anit-Aging Research Center, Dong-eui University) ;
  • Yung Hyun Choi (Anit-Aging Research Center, Dong-eui University) ;
  • Su Hyun Hong (Anit-Aging Research Center, Dong-eui University)
  • 김민영 (동의대학교 항노화연구소) ;
  • 최영현 (동의대학교 항노화연구소) ;
  • 홍수현 (동의대학교 항노화연구소)
  • Received : 2024.05.04
  • Accepted : 2024.05.29
  • Published : 2024.05.31
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Abstract

Objective : Inulae Flos(IF) has been used to treat arthritis, sever furuncle, fear and palpitation, vomiting, stroke, asthma and cough in Korean Medicine. Although the anticancer activity of IF has been reported, the molecular mechanism is still not well understood. In this study, we investigated the growth inhibitory activity of an ethanol extract of IF in HT-1080 human fibrosarcoma cells and its underlying mechanisms using two-dimensional (2D) and three-dimensional (3D) cell culture system. Methods : HT-1080 cells were cultured with IF for 9 days in 3D cell culture. To check an inhibition of cell prolifelation by IF, MTT assay was performed. DNA contents were measured using flow cytometry. Western blotting was used to evaluate the regulation of cell cycle- and autophagy-related proteins. Acridine orange staining was performed to confirm autophagy, and DCF-DA staining was performed to confirm the occurrence of ROS. Results : IF controlled a spheroid formation and decreased a cell viability in 3D cell culture. IF-induced cell proliferation inhibition was associated with a distinct increase of S and G2/M phase cell distribution in 2D cell cultre. In addition, IF significantly induced autophagy and generated reactive oxygen species(ROS). Interestingly, IF-induced cell cycle arrest and autophagy were recovered after pre-treatment of N-acetyl-L-cysteine, ROS scavenger. Conclusion : Our results indicate that IF induced ROS-mediated cell cycle arrest and autophagy and it may potentially useful for human fibrosarcoma treatment.

Keywords

Acknowledgement

이 논문은 정부(과학기술정보통신부)의 재원으로 한국연구재단에서 시행한 기초연구사업 (No. 2022R1A2C1003573) 및 2023학년도 동의대학교 연구년 지원에 의하여 연구되었습니다.

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