• Title/Summary/Keyword: Thiosulfate

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Purification and Characterization of Oxidase Induced from Thiobacillus neapolitanus R-10 (Thiobacillus neapolitanus R-10으로 부터 산화 효소의 분리 정제와 특성)

  • Weon, Yong-Don;Kim, Dong-Suck;Ryu, Beung-Ho
    • Journal of Environmental Science International
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    • v.5 no.1
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    • pp.43-50
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    • 1996
  • Thiobacillus neopolitanus R-10, which produces a active thiosulfate oxidase was isolated from nightsoil. The optimal culture conditions of Thiobacillus neopolitanus R-10 for the production of enzyme was determined as followed: 0.8% $Na_2S_2O_3$, 0.2% $KH_2PO_4$, 0.2% $K_2HPO_4$, 0.04% $Na_2CO_3$, 0.02% $MgSO_4$.$7H_2O$, 2ml trace elements solution, ann PH 6.5 at 3$0^{\circ}C$ and 72hr cultivation. The oxidase was successively purified 83 folds yield by ${(NH_2)}_2SO_4$ fractionation, DEAE-Cellulose, Sephadex A-50 column chromatogrophy and gel Sephadex G-150 gel filteration with yield of 5.9%. The molecular weight of purified enzyme was estimated to be 43.000 dalton by SDS-polyacrylamide gel electrophoresis and gel filteration column chromatography The enzyme activity was highest at 40t and PH 7.0 The enzyme activity was relatively high by $\beta$-mercaptoethanol but strongly inhibited by cysteine.

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Chemical Leaching of Silver from Diverse Resources (다양한 자원으로부터 은의 화학적 침출)

  • Xing, Weidong;Lee, Manseung
    • Resources Recycling
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    • v.26 no.1
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    • pp.3-10
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    • 2017
  • The special properties of silver are often indispensable in the manufacture of advanced materials. Therefore, it is of importance to develop a process to recover silver which is necessary for the production of advanced materials from diverse resources. In this manuscript, the developed processes for the leaching of silver from diverse resources are reviewed. For this purpose, the advantages and disadvantages of using some inorganic acids (nitric and sulfuric acid) and their mixture with other oxidizing agents (ozone, oxygen, hydrogen peroxide and ferric ion) were investigated. Moreover, the leaching of silver with thiourea and thiosulfate was compared over those by inorganic acids in terms of environmental effect.

Separation of Gold and Silver from Diverse Solutions by Solvent Extraction (다양한 용액으로부터 용매추출에 의한 금과 은의 분리)

  • Xing, Weidong;Lee, Manseung
    • Resources Recycling
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    • v.26 no.5
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    • pp.3-11
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    • 2017
  • Solvent extraction is an important process to recover pure gold and silver from various leaching solutions. The present work reviews the aqueous chemistry and solvent extraction separation of gold (I, III) and silver (I) from several leaching systems such as cyanide, thiocyanate, thiosulfate, thiourea and chloride medium. The extraction and separation behavior of gold (I, III) and silver (I) by various single and mixtures was compared on the basis of extraction reaction and the selectivity from these mediums. The chloride medium is recommended for the separation of gold and silver by solvent extraction in terms of extraction and stripping efficiency.

Ethylene Production and Internal Structure of Developing Maize Seeds (옥수수 종자의 발육 중 ethylene 발생과 내부형태 변화)

  • Lee Suk-Soon;Seo Jung-Moon;Hong Seung-Beom
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.5
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    • pp.425-431
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    • 2006
  • In order to investigate the effects of ethylene on the seed development of three corn types (dent, sweet, and super sweet corns), aminoethoxyvinyl glycine (AVG) and silver thiosulfate (STS) and ethephon (2-chloroethylphosphonic acid, CEPA) were applied either on whole plants or shanks of ears at 9 and 21 days after silking. Ethylene production of developing super sweet corn seeds was much higher than those of sweet and dent corns. The cavity in the endosperm tissues of the super sweet corn started earlier and endosperm was collapsed more severely compared to those of sweet and dent corns. Ethylene production seemed to be related to the death of endosperm cells to form a cavity. Application of AVG and STS reduced ethylene production and delayed cavity formation in endosperm of super sweet corn seeds, while CEPA increased ethylene production and enhanced the time of cavity formation. AVG and STS increased 100-seed weight, while CEPA decreased.

Effect of Uniconazole and Silver Thiosulfate Treatment on Reduction of Ozone Injury in Snap Bean Plants (Uniconazole과 Silver Thiosulfate 처리(處理)가 강남콩의 오존피해(被害) 경감(輕減)에 미치는 효과(效果))

  • Ku, Ja Hyeong;Won, Dong Chan;Cho, Jeong Hee;Shin, Dae Shik
    • Korean Journal of Agricultural Science
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    • v.19 no.2
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    • pp.161-169
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    • 1992
  • Studies were conducted to examine the effects of single or combined treatment of uniconazole [(E)-1-(4-chlorophenyl)-4, 4-dimethyl 2(1, 2,-4-triazol-1-yl)-1-penten-3-ol)] and silver thiosulfate (STS) on reducing ozone injury to snap beans (Phaseolus vulgaris L. 'Strike'). Two weeks after seeding, plants were given a soil drench of uniconazole(XE-1019) solution at concentrations of 0.001, 0.005 and 0.025 mg/pot, and then two days prior to ozone fumigation, 0.3 and 0.6 mM STS containing 0.01% Tween-20 were also sprayed. Uniconazole was effective in providing protection against ozone injury through increase activities of free radical scavengers such as superoxide dismutase (SOD) and peroxidase (POD) as well as the increase of chlorophyll content and stomatal resistance resulted from plant growth retardation. The phytoprotective effects of STS seemed to be related to its properly of blocking the ethylene action and increasing activities of SOD and POD. Even at low concentrations, a combined treatment with uniconazole drench, STS spray significantly reduced ozone injury compared to single application.

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Sorting of Aged Seeds Using Leakage Substances of Crop Seeds (종자 누출물질을 이용한 노화종자의 선별)

  • Lee, Dong-Jin;Lim, Moo-Sang;Chu, Young-Ha;Chung, Kil-Woong;Kim, Bong-Ku
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.783-789
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    • 1997
  • This study was conducted to identify leakage of fluorescent substances out of 56 crops and to develop sorting methods of nonviable seeds of Chinese cabbage. Fluorescent substances were detected from 36 species, Chinese cabbage, radish, broccoli, cauliflower, egg plant and rape seed etc. In Chinese cabbage, the germination rate of non-sorted seeds decreased rapidly with ageing of seeds at 45$^{\circ}C$ and 100%. After Chinese cabbage seeds soaked for 4hrs and coated with cellulose, the sorting ratio of non fluorescent seeds were decreased 91.3% to 1.7% by increasing ageing periods from control to 8 days, while fluorescent seeds were increased drastically from 8.7% to 98.3%. Also, incubation of water soaked dead seeds of Chinese cabbage treated with 0.5-1.0% ninhydrin and 0.01N sodium thiosulfate for 30~60 minutes at 35$^{\circ}C$ developed purple color.

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The synthesis and antineoplastic activity of alkyl thiosulfates and diaryl disulfides

  • Chae, Dong-Kyu;Chung, Won-Keun;Lee, Nam-Bock
    • YAKHAK HOEJI
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    • v.16 no.3
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    • pp.149-154
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    • 1972
  • Seven alkylthiosulfates and three diaryl thiosulfates were synthesized as potential antineoplastic agents, and subjected to antineoplastic activity test against Ehrlich ascites carcinoma, SN-36 Leukemia and Sarcoma 180. Allyl thiosulfate, n-propylthiosulfate and ${\beta}$-hydroxyethylthiosulfate were found as to be active against experimental tumors.

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Effects of Temperature and Ethylene Response Inhibitors on Growth and Flowering of Passion Fruit

  • Liu, Fang-Yin;Peng, Yung-Liang;Chang, Yu-Sen
    • Horticultural Science & Technology
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    • v.33 no.3
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    • pp.356-363
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    • 2015
  • This study examined the effects of different day/night temperature regimes or silver ion on growth and flowering of passion fruit 'Tai-nung No.1'. Low temperature treatment ($20/15^{\circ}C$) caused passion fruit cultivar 'Tai-nung No.1' to fail to flower. Flowering induction occurred within a temperature range of $20-30^{\circ}C$, with no significant difference in the days to first flower bud and the total number of flower buds between plants grown at $30/25^{\circ}C$ and $25/20^{\circ}C$. However, plants grown at $30/25^{\circ}C$ exhibited their first flower buds set on the higher nodes and had higher abortion rates of flower buds than those at $25/20^{\circ}C$. Plants grown at $30/25^{\circ}C$ had the most rapid growth and the shortest plastochron. We also evaluated the effect of the ethylene response inhibitors silver nitrate ($AgNO_3$) and silver thiosulfate (STS) on growth and flowering of potted passion fruit 'Tai-nung No.1', when they were exposed to low temperature conditions ($20/15^{\circ}C$) following chemical treatments ($AgNO_3$ or STS, at 0.5 or 1.0 mM). $AgNO_3$ and STS treatments induced flower formation and initial flower bud formation within approximately two weeks at $20/15^{\circ}C$ whereas non-treated control plants exhibited no flower formation. ACC content and activity of ACC oxidase in the leaves of passion fruit 'Tai-nung No.1'exposed to low temperature conditions ($20/15^{\circ}C$) were significantly inhibited by the ethylene inhibitor treatments. These results indicate that ethylene, which is produced under low temperature conditions, plays an important role in inhibiting flower formation in passion fruit.