• 한국약용작물학회:학술대회논문집
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• 2012.05a
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• pp.153-154
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• 2012

• Korean Journal of Microbiology
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• v.21 no.1
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• pp.27-35
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• 1983

The stoichiometry between the consumption of CO and $O_2$ and the production of $CO_2(2CO+O_2{\rightarrow}2CO_2)$) showed that Pseudomonas carboxydohydrogena grows as a typical aerobic CO oxidizer with CO. The optimal concentration of CO for growth was found to be 30% in gas mixture with air. The initial buffer concentration of the culture medium did not affect the growth of this bacterium. P. carboxydohydrogena is an obligate aerobe and dose not use nitrate as a terminal electron acceptor. The CO dehydrogenase is an inducible and soluble enzyme. The reaction rate and stability were maximal at pH7.5, and the Arrhenius plot revealed an activation energy of 37.7kJ/mol (9.0 Kcal/mol). The crude enzyme used methylene blue, thionin, and toluylene blue as electron acceptors for the oxidation of CO to $Co_2$ under anaerobic conditions. It was found that water must be the source of the second oxygen atom for CO oxidation.

• The Korean Journal of Zoology
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• v.10 no.1
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• pp.10-16
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• 1967

본 연구는 백서후각기의 발생과정을 세포조직학적으로 규명보고하는 바이며 사용된 stage는 stage 20부터 32까지는 Christe(1964)의것을 인용하였으며 그 외에 명시된 stage 33,34, 35 는 저자가 편의상 구분하였다. 재료는 백서 5 필을 온실서 충분한 영양으로 사육후 임신시켜 요구되는 배를 stage 별로 얻어 Bouin's solution 에 고정시켜 paraffin법을 따랐으며 5-7$\mu$의 두께로 절편을 만들어 Delafield's hematoxylin, eosin, thionin 에 염색하였다. Stage 별 후각기의 발생은 state 20 에 후순, state 22에 후와 및 조비기(Jacobson's organ), stage23A에 구비강막(oro-nasal membrane), state 23 B 에 수신경섬유 와 원시내비공, stage 24 에 조비기신경섬유, stage 27에는 골갑개상피에 후세포와 호흡상피상에 섬유 또한 악골갑개상피에서 seromucinous 선들이 최초로 나타났다. 본 실험결과 백서후각기의 형성과정은 인체와 생쥐( mouse) 에서와 유사하나 조비기의 성장, 비갑개의수, 및 후역의 범위에 있어서는 극히 차가 심한 것같다.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.64.1-64
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• 2003

Inoculation of primary pepper leaves with an avirulent strain of Xanthomonas campestris pv. vesicatoria induced systemic acquired resistance (SAR) in secondary leaves. This SAR response was accompanied by the systemic expression of defense-related genes, a systemic microoxidative burst generating H2O2, and the systemic induction of ion-leakage and callose deposition in the non-inoculated, secondary leaves. Some defense-related genes encoding PR-1, chitinase, peroxidase, PR10, thionin, defensin and zinc-finger protein were distiilctly induced in the systemic leaves. The systemically striking accumulation of H$_2$O$_2$and strong increase in peroxidase activity in pepper was suggested to contribute to the triggering of cell death In the systemic micro-HRs, leading to the induction of SAR. Treatment of non-inoculated, secondary leaves with diphenylene iodinium (DPI), an inhibitor of the oxidative burst, substantially reduced the induction of some defense-related genes and subsequently SAR.

• International Journal of Industrial Entomology and Biomaterials
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• v.22 no.1
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• pp.21-23
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• 2011

In order to produce the colored cocoons we finalized the adequate amount of dyes throughout several trial and error experiments. The proper amount of each dye required for per 100 g of each colored cocoon turned out to be 150 mg of Rhodamine, 1560 mg of Thionin, 170 mg of Neutral red, and 200 mg of N-Blue, respectively. With this amount of dyes silkworms grew without physiological disorders. In order to produce colored cocoons, artificial diet composed mainly of mulberry leaves was fed to silkworms from the beginning of 5th instar, and subsequently fed with dye included diet from $4^{th}$ day of the 5th instar. This process resultantly produced colored silkworm body from the onset of feeding and subsequently colored cocoons and eggs. Nevertheless, the dye induced color was not inherited to next generation.

• Bulletin of the Korean Chemical Society
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• v.27 no.2
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• pp.281-285
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• 2006

Microbial fuel cells (MFC) stacked with bipolar plates have been constructed and their performance was tested. In this design, single fuel cell unit was connected in series by bipolar plates where an anode and a cathode were made in one graphite block. Two types of bipolar plate stacked MFCs were constructed. Both utilized the same glucose oxidation reaction catalyzed by Gram negative bacteria, Proteus vulgaris as a biocatalyst in an anodic compartment, but two different cathodic reactions were employed: One with ferricyanide reduction and the other with oxygen reduction reactions. In both cases, the total voltage was the mathematical sum of individual fuel cells and no degradation in performance was found. Electricity from these MFCs was stored in a supercapacitor to drive external loads such as a motor and electric bulb.

• Korean Journal of Veterinary Service
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• v.30 no.3
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• pp.363-374
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• 2007

A biochemical characterization and antimicrobial drugs susceptibility study was conducted in four breeding kennel which was canine abortion caused by Brucella canis in Gyeongbuk province in 2003-2006. Total of 267 dogs domesticated in the four kennel were examination. Among them, 143 (53.6%) dogs were sero-positive and 25 of blood samples were isolated to Brucella canis. At amplification of 35KDa-BCSP gene using PCR, 711 bp DNA fragment was same visible in 25 isolates and B canis RM6/66. Biochemical characterization of B canis isolated was non-hemolytic, no production of $H_2S$, no fermentation of carbohydrates, catalase-positive, oxidase-positive, indol-negative, hydrolyzation of urea, reduction of nitrate and development of thionin dye medium. Using disk-diffusion method, all of 25 strains tested were found to be highly susceptible to tetracycline, aminoglycoside, quinolone, macrolide antibiotics, rifampin and ampicillin in vitro. Using PFGE with restriction enzyme Smi I, 25 isolates tested were typed to 2 pattern, S1 and S2.

• Korean Journal of Veterinary Service
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• v.33 no.2
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• pp.129-134
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• 2010

Canine brucellosis is a contagious disease of the reproductive tract that cause mainly abortion and infertility in dog. A serological and bacteriological survey was conducted for breeding kennels which were suffered from frequent outbreak of canine brucellosis in Gyeongbuk province in 2009. Among 138 samples, 45 serum samples were sero-positive. Brucella canis was isolated from 30 blood samples of the seropositive cases, and from 2 samples of 62 sero-negatives. The biochemical properties of 32 isolates were characterized with no production of H2S, no fermentation of carbohydrates, hydrolyzation of urea, and development of thionin dye medium. At amplification of BCSP and 16S-rRNA gene using PCR, 711bp and 905bp DNA fragments were detected in agarose. Three tandem repeat pattern was shown in genotyping by Multi-locus VNTR assay (MLVA).

• Korean Journal of Veterinary Research
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• v.29 no.1
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• pp.1-6
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• 1989

The number and distribution of the retinal ganglion cells in the 2 years old Korean native cattle was determined from whole fiat mounted preparation stained with methylene blue and thionin. The results were summarized as follows. 1. The total number of retinal ganglion cells was estimated to be 3,085,200 in the bovine retina ranging from $2,214mm^2$ in total area. 2. Visual streak was recognized at the area 2.5mm superior to the optic disc and ganglion cell density drops off rapidly to the directions superior to and inferior to the visual streak. 3. Area centralis ($6,800cells/mm^2$) was located at the area 10mm temporally from the point of 3mm superior to the optic disc. 4. The number of ${\alpha}-type$ ganglion cells (above $15{\mu}$) was 57,000 in the bovine retina and ${\alpha}-type$ ganglion cells constituted 18.5% of the total cells. 5. The relative frequency of ${\alpha}-type$ ganglion cells was higher in the peripheral regions than in the visual streak, especially higher in the superior-temporal quadrant than in other region of the bovine retina.

• Journal of Life Science
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• v.28 no.9
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• pp.1030-1041
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• 2018

Abiotic stresses limit the growth and productivity of plants. Cellular adaptation to abiotic stresses requires coordinated regulation in gene expression directed by complex mechanisms. This study used the activation tagging system to identify novel salt stress-responsive genes. The study selected 9 activation tagging lines that showed salt stress-tolerant phenotypes during their germination stages. Thermal asymmetric interlaced-PCR (TAIL-PCR) was used to identify the T-DNA tagging sites on the Arabidopsis genome in selected activation tagging lines, including AT7508, AT7512, AT7527, AT7544, AT7548, and AT7556. RT-PCR analysis showed that ClpC2/HSP93-III (At3g48870), plant thionin family (At2g20605), anti-muellerian hormone type-2 receptor (At3g50685), vacuolar iron transporter family protein (At4g27870), and microtubule-associated protein (At5g16730) were activated in AT7508, AT7512, AT7527, AT7544, and AT7556, respectively. Interestingly, in AT7548, both the genes adjacent to the T-DNA insertion site were activated: Arabinogalactan protein 13 (AGP13) (At4g26320) and F-box/RNI-like/FBD-like domains-containing protein (At4g26340). All of the seven genes were newly identified as salt stress-responsive genes from this study. Among them, the expression of ClpC2/HSP93-III, AGP13, F-box/RNI-like/FBD-like domains-containing protein gene, and microtubule-associated protein gene were increased under salt-stress condition. In addition, AT7508, AT7527, and AT7544 were more tolerant to salt stress than wild type at seedling development stage, functionally validating the screening results of the activation tagging lines. Taken together, our results demonstrate that the activation tagging system is useful for identifying novel stress-responsive genes.