• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
• /
• pp.273-273
• /
• 2013

Recently, Point-of-care (POC) testing microdevices enable to do the patient monitoring, drug screening, pathogen detection in the outside of hospital. Immunochromatographic strip (ICS) is one of the diagnostic technologies which are widely applied to POC detection. Relatively low cost, simplicity to use, easy interpretations of the diagnostic results and high stability under any circumstances are representative advantages of POC diagnosis. It would provide colorimetric results more conveniently, if the genetic analysis microsystem incorporates the ICS as a detector part. In this work, we develop a reverse transcriptase-polymerase chain reaction (RT-PCR) microfluidic device integrated with a ROSGENE strip for colorimetric influenza H1N1 virus detection. The integrated RT-PCR- ROSGENE device is consist of four functional units which are a pneumatic micropump for sample loading, 2 ${\mu}L$ volume RT-PCR chamber for target gene amplification, a resistance temperature detector (RTD) electrode for temperature control, and a ROSGENE strip for target gene detection. The device was fabricated by combining four layers: First wafer is for RTD microfabrication, the second wafer is for PCR chamber at the bottom and micropump channel on the top, the third is the monolithic PDMS, and the fourth is the manifold for micropump operation. The RT-PCR was performed with subtype specific forward and reverse primers which were labeled with Texas-red, serving as a fluorescent hapten. A biotin-dUTP was used to insert biotin moieties in the PCR amplicons, during the RT-PCR. The RT-PCR amplicons were loaded in the sample application area, and they were conjugated with Au NP-labeled hapten-antibody. The test band embedded with streptavidins captures the biotin labeled amplicons and we can see violet colorimetric signals if the target gene was amplified with the control line. The off-chip RT-PCR amplicons of the influenza H1N1 virus were analyzed with a ROSGENE strip in comparison with an agarose gel electrophoresis. The intensities of test line was proportional to the template quantity and the detection sensitivity of the strip was better than that of the agarose gel. The test band of the ROSGENE strip could be observed with only 10 copies of a RNA template by the naked eyes. For the on-chip RT-PCR-ROSGENE experiments, a RT-PCR cocktail was injected into the chamber from the inlet reservoir to the waste outlet by the micro-pump actuation. After filling without bubbles inside the chamber, a RT-PCR thermal cycling was executed for 2 hours with all the microvalves closed to isolate the PCR chamber. After thermal cycling, the RT-PCR product was delivered to the attached ROSGENE strip through the outlet reservoir. After dropping 40 ${\mu}L$ of an eluant buffer at the end of the strip, the violet test line was detected as a H1N1 virus indicator, while the negative experiment only revealed a control line and while the positive experiment a control and a test line was appeared.

• 공업화학
• /
• 제34권3호
• /
• pp.207-212
• /
• 2023

근적외선 흡수 염료는 전통적으로 정보 기록 및 정보 표시 분야에 사용되었을 뿐만 아니라 최근 광학 필터, 바이오, 에너지 저장 및 변환, 코팅 첨가제 등 다양한 응용 분야에 적용되고 있다. 핸드폰이나 디지털 카메라에 사용되는 이미지 센서는 근적외선 영역에서도 감도를 나타내기 때문에 보다 선명한 이미지를 구현하기 위해 근적외선 광학필터가 필수적이다. 에너지 저장 및 변환이 중요해짐에 따라 투명 태양 전지 분야에서는 근적외선 영역까지 흡수 영역을 확장할 수 있도록 다양한 근적외선 흡수 소재가 개발되고 있으며, 이를 이용해 소자 효율을 향상시키는 연구가 진행되고 있다. 미래 모빌리티 기술로 많은 관심을 받고 있는 자기치유 코팅 시스템에 광-열 효과를 갖는 근적외선 흡수 염료가 도입되어 보다 효율적인 자기치유 성능을 구현하는 연구들도 보고되고 있다. 본 총설에서는 대표적인 근적외선 흡수 염료들의 화학 구조들을 소개하고, 근적외선 흡수 염료들을 기반으로 한 최신 응용 연구 동향에 대해 다뤄보고자 한다.

• 센서학회지
• /
• 제6권1호
• /
• pp.6-15
• /
• 1997

이 논문은 빔의 구조를 갖거나 멤브레인의 구조를 갖는 써모파일 센서의 다목적 최적설계에 관한 연구이다. 연구대상의 써모파일 센서는 $Si_{3}N_{4}/SiO_{2}$ 박막위에 알루미늄과 다결정 실리콘을 사용하여 열전쌍을 형성하고, 박막중심부에 $RuO_{2}$를 사용하여 적외선 흡수부를 만들어 중심부와 실리콘림부 사이의 온도차이에 따른 Seebeck 효과에 의한 유기전압을 감지하는 센서를 대상으로 하였다. 최적설계의 목적함수는 센서의 감도, 검출능 (detectivity) 및 열시정수를 대상으로 하였다. 패키지를 고려하여 모델링을 하였으며, 기존의 식의 고찰에 의한 단순 설계방법이 아닌 수학적 계획법을 사용한 다목적 최적화 방법을 이용하여 최적해를 구하였다. 최종적인 최적설계 수식화에는 퍼지계획법에서 사용되는 소속함수를 정의하여 설계자가 우선적으로 신뢰할 수 있는 해를 구 할 수 있도록 하였다. 또한, 제한조건으로서 주위 온도변화에 따른 센서의 출력전압변화를 포함시켜 실제 사용되는 환경을 고려하였다.

• The Plant Pathology Journal
• /
• 제31권3호
• /
• pp.219-225
• /
• 2015

The primary step for efficient control of viral diseases is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been used to detect viral RNA molecules because of its simplicity and high sensitivity for a number of viruses. RT-LAMP for the detection of Potato virus X (PVX) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to demonstrate its advantages over RT-PCR. RT-LAMP reactions were conducted with or without a set of loop primers since one out of six primers showed PVX specificity. Based on real-time monitoring, RT-LAMP detected PVX around 30 min, compared to 120 min for RT-PCR. By adding a fluorescent reagent during the reaction, the extra step of visualization by gel electrophoresis was not necessary. RT-LAMP was conducted using simple inexpensive instruments and a regular incubator to evaluate whether RNA could be amplified at a constant temperature instead of using an expensive thermal cycler. This study shows the potential of RT-LAMP for the diagnosis of viral diseases and PVX epidemiology because of its simplicity and rapidness compared to RT-PCR.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
• /
• pp.278-278
• /
• 2016

Solution-processed metal-oxide semiconductors have been considered as the next generation semiconducting materials for transparent and flexible electronics due to their high electrical performance. Moreover, since the oxide semiconductors show high sensitivity to light illumination and possess persistent photoconductivity (PPC), these properties can be utilized in realizing optical memory devices, which can transport information much faster than the electrons. In previous works, metal-oxide semiconductors are utilized as a memory device by using the light (i.e. illumination does the "writing", no-gate bias recovery the "reading" operations) [1]. The key issues for realizing the optical memory devices is to have high photoconductivity and a long life time of free electrons in the oxide semiconductors. However, mono-layered indium-zinc-oxide (IZO) and mono-layered indium-gallium-zinc-oxide (IGZO) have limited photoconductivity and relaxation time of 570 nA, 122 sec, 190 nA and 53 sec, respectively. Here, we boosted up the photoconductivity and relaxation time using a double-layered IZO/IGZO active layer structure. Solution-processed IZO (top) and IGZO (bottom) layers are prepared on a Si/SiO2 wafer and we utilized the conventional thermal annealing method. To investigate the photoconductivity and relaxation time, we exposed 9 mW/cm2 intensity light for 30 sec and the decaying behaviors were evaluated. It was found that the double-layered IZO/IGZO showed high photoconductivity and relaxation time of 28 uA and 1048 sec.

• 한국표면공학회지
• /
• 제37권2호
• /
• pp.71-75
• /
• 2004

The technology of $C_{60}$ fullerite films preparation by means of gas-phase deposition and structure of fullerite films are described. A three-channel flow plant was used to obtain fullerite films. The films were deposited in the flow of inert gas under reduced pressure onto a cooled silicon or sapphire substrate placed inside the reaction chamber of the plant. The plant allows one to obtain the films of pure fullerenes and to synthesise the films from fullerene compounds and doped fullerenes. The structure of two types of films were investigated by FE-SEM and SEM techniques: pure fullerite films onto silicon and sapphire substrates as well as compound films were studied by FE-SEM technique. All samples have shown columnar structure with high level of porosity. The synthesis of films composed of fullerene and its compounds for use in electronics is demonstrated to be promising. For example, experiments confirm the possibility to use fullerite films in sensor electronics to produce humidity and thermal sensors. It is also possible to use the sensitivity of these films to isotropic pressure. The experiments with $C_{60}$-Cu-J films have shown quite strong dependence of their resistance on pressure of different sort of medium-gas that could be used in gas-sensitive sensors. The structure and preparation technology of resistive sensor based on fullerite films are described.bed.

• 에너지공학
• /
• 제11권2호
• /
• pp.166-177
• /
• 2002

본 연구는 화염안정을 위해 약간의 메탄을 첨가한 1차원, Hat, 예혼합, 층류 석탄-공기 화염구조에 관한 연구로서 반응영역을 늘리기 위해 0.3 atm에서 운전되는 저압버너를 사용하였다. 본 연구에서는 가스 온도, 주요가스의 농도, 샘플된 촤의 분석과 화염속도에 대하여 여러 모델들의 해석결과를 실험결과와 서로 비교하였다. 여러 모델중 촤 표면적 지수(S=4)와 휘발성분에 대해 각각의 탈휘발화 속도상수를 적용한 model II $I^{*}$ -d가 실험치와 비교적 일치함을 보여주었다. 샘플된 촤의 분석 결과 입자의 반응이 낮게 예측되어져 촤 표면적지수를 증가시켜야만 했다. 이 지수는 촤의 반응 표면적에 대한 민감도 분석으로부터 얻어진 결과였고 model II $I^{*}$ -d의 화염속도 해석결과는 대부분의 측정치에 근접한 결과를 보여주고 있다. 고체 입자 직경은 열적 지연과 반응표면적을 통하여 탈휘발화율과 촤 산화에 큰 영향을 주며 이는 곧 화염속도에 영향을 주고 있음을 보여주었다.

• 한국시스템다이내믹스연구
• /
• 제9권2호
• /
• pp.129-154
• /
• 2008

As a practical means to upgrade urban sustainability, this paper focuses on resource cyclical systems concerned with the waste of electric and electronic equipment (WEEE) in Korea. Borrowing System Dynamics concepts and approaches, it examines behavioral changes of WEEE dynamics to observe whether the existing management methods can be readjusted. The measurement is based upon both reuse and material and thermal recycle simulation works in the individual stage of WEEE discharge, collection, and treatment, going beyond the traditional recycle-only customs. This research estimates that the newly introduced Extended Producer Responsibility (EPR) system would definitely exert a significant impact on the final stage of WEEE treatment, decreasing the final treatment volume in the first half of the research period. The trend, nonetheless, would be reversed in the second half, mainly owing to the additional waste volume originated from the local government and recycling center. Sensitivity analysis poses, among others, that the local government-supported reuse center should take charge of a pivotal role in the long run. The research also shows that sufficientand necessary conditions for the WEEE management and treatment should be given to the combined efforts, both from the private sectors and the public domains. Based on these research findings, the paper recommends that key stakeholders including the producer and the public organizations should devise how to jointly carry out specific agenda centered around partnership or network buildings.

• International Journal of Aeronautical and Space Sciences
• /
• 제17권1호
• /
• pp.37-44
• /
• 2016

Optical fiber temperature sensing systems have incomparable advantages over traditional electrical-cable-based monitoring systems. However, the fiber optic interrogators and sensors have often been rejected as a temperature monitoring technology in real-world industrial applications because of high cost and over-specification. This study proposes a multiplexed fiber optic temperature monitoring sensor system using an economical Optical Time-Domain Reflectometer (OTDR) and Hard-Polymer-Clad Fiber (HPCF). HPCF is a special optical fiber in which a hard polymer cladding made of fluoroacrylate acts as a protective coating for an inner silica core. An OTDR is an optical loss measurement system that provides optical loss and event distance measurement in real time. A temperature sensor array with the five sensor nodes at 10-m interval was economically and quickly made by locally stripping HPCF clad through photo-thermal and photo-chemical processes using a continuous/pulse hybrid-mode laser. The exposed cores created backscattering signals in the OTDR attenuation trace. It was demonstrated that the backscattering peaks were independently sensitive to temperature variation. Since the 1.5-mm-long exposed core showed a 5-m-wide backscattering peak, the OTDR with a spatial resolution of 40 mm allows for making a sensor node at every 5 m for independent multiplexing. The performance of the sensor node included an operating range of up to $120^{\circ}C$, a resolution of $0.59^{\circ}C$, and a temperature sensitivity of $-0.00967dB/^{\circ}C$. Temperature monitoring errors in the environment tests stood at $0.76^{\circ}C$ and $0.36^{\circ}C$ under the temperature variation of the unstrapped fiber region and the vibration of the sensor node. The small sensitivities to the environment and the economic feasibility of the highly multiplexed HPCF temperature monitoring sensor system will be important advantages for use as system-integrated temperature sensors.

• 대한원격탐사학회지
• /
• 제27권6호
• /
• pp.653-662
• /
• 2011

Land surface temperature (LST) is a one of the key variables of land surface which can be estimated from geostationary meteorological satellite. In this study, we have developed the three sets of LST retrieval algorithm from MTSAT-2 data through the radiative transfer simulations under various atmospheric profiles (TIGR data), satellite zenith angle, spectral emissivity, and surface lapse rate conditions using MODTRAN 4. The three LST algorithms are daytime, nighttime and total LST algorithms. The weighting method based on the solar zenith angle is developed for the consistent retrieval of LST at the early morning and evening time. The spectral emissivity of two thermal infrared channels is estimated by using vegetation coverage method with land cover map and 15-day normalized vegetation index data. In general, the three LST algorithms well estimated the LST without regard to the satellite zenith angle, water vapour amount, and surface lapse rate. However, the daytime LST algorithm shows a large bias especially for the warm LST (> 300 K) at day time conditions. The night LST algorithm shows a relatively large error for the LST (260 ~ 280K) at the night time conditions. The sensitivity analysis showed that the performance of weighting method is clearly improved regardless of the impacting conditions although the improvements of the weighted LST compared to the total LST are quite different according to the atmospheric and surface lapse rate conditions. The validation results of daytime (nighttime) LST with MODIS LST showed that the correlation coefficients, bias and RMSE are about 0.62~0.93 (0.44~0.83), -1.47~1.53 (-1.80~0.17), and 2.25~4.77 (2.15~4.27), respectively. However, the performance of daytime/nighttime LST algorithms is slightly degraded compared to that of the total LST algorithm.