• Plant Biotechnology Reports
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• v.3 no.1
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• pp.95-101
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• 2009

Tea leaves are major source of catechins—antioxidant flavonoids. Dihydroflavonol 4-reductase (DFR, EC 1.1.1.219) is one of the important enzymes that catalyzes the reduction of dihydroflavonols to leucoanthocyanins, a key ''late'' step in the biosynthesis of catechins. This manuscript reports characterization of DFR from tea (CsDFR) that comprised 1,413 bp full-length cDNA with ORF of 1,044 bp (115-1,158) and encoding a protein of 347 amino acids. Sequence comparison of CsDFR with earlier reported DFR sequences in a database indicated conservation of 69-87% among amino acid residues. In silico analysis revealed CsDFR to be a membrane-localized protein with a domain (between 16 and 218 amino acids) resembling the NAD-dependent epimerase/dehydratase family. The theoretical molecular weight and isoelectric point of the deduced amino sequence of CsDFR were 38.67 kDa and 6.22, respectively. Upon expression of CsDFR in E. coli, recombinant protein was found to be functional and showed specific activity of 42.85 nmol $min^{-1}$ mg $protein^{-1}$. Expression of CsDFR was maximum in younger rather than older leaves. Expression was down-regulated in response to drought stress and abscisic acid, unaffected by gibberellic acid treatment, but up-regulated in response to wounding, with concomitant modulation of catechins content. This is the first report of functionality of recombinant CsDFR and its expression in tea.

• Architectural research
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• v.16 no.2
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• pp.37-44
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• 2014

Utilizing spaces for awareness could be superficially noble. However, the typology of spaces played an important role in addressing the awareness of translating the messages through tangible forms, which in this context are sculptures. Assuring the efficacy of the typology of the spaces that is compatible with the content is important to ensure its timelessness to the dynamic demographical change of viewers. Therefore, the study proposes an investigation of the efficacy of typology of public urban space; garden that accommodates memorial sculptures. The study intends to analyze the features and flaws of ASEAN Sculpture Garden, as a mediatory role of ASEAN in instilling awareness of unity between ASEAN members to the public. The objective of the study is to analyze the efficacy of the garden to accommodate the rich memorial sculptures. The body of the research is formed by theoretical and case study research. It is projected by three methods; archival research, semi structured interviews and site documentation; direct observations and site visit. The qualitative data then will be analysed using Actor Network theory, keyword coding and site findings. Findings showed that as the main objective of the garden is to exhibit the sculptures, it could be summed up that it has failed to meet the apparent intention based on the site analysis conducted by the researcher. The typology of the space should be able to communicate the project's aims continuously rather than serving the function temporarily. The variables, which are space and time factors are debatable, as the neighboring tourist spot, National Monument residing the wide square managed to attract public attention due to its great efficacy of typological space regardless the time factor. As a conclusion, the typology of space is a huge factor to ensure the park's success.

• Korean Journal of Social Welfare
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• v.62 no.3
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• pp.323-351
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• 2010

Through the biographical approach, the current research purports to reconstruct the Changeprocess of values and identities on the lives of the first-generation Korean-German females in Germany from the transnational perspective. Ten interviews were conducted, using Schutze's autobiographicalnarrative interview. Interview data were analyzed through the application of Schutze's autobiographical-narrative interview and Mayring's qualitative content analysis. Findings showed that on the onset of emigration, their values centered around hybrid collectivism. Their life in the process of emigration was characteristic of a shift to hybrid individualism. Furthermore, the life at beginning of emigration was found to be characterized by a singular regional identity. The process of emigration was shown to mark the conversion into dual identity, dual regional and dual national. Some theoretical and practical suggestions for the emigrants' welfare were finally offered that were associated with the process of values and identities changes in their life.

• The Journal of the Korea Contents Association
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• v.16 no.7
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• pp.566-574
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• 2016

The purpose of this study was to determine the viewing trends in eco-childhood education. Analyte is analyzed, such as from 10 March 2008 and 2015 annual target of 97 pieces of thesis until March trends, research content, research methods. As a result, first, the ecological childhood education subjects infant destination, Teachers, took place in indoor and outdoor environments subject, parent targeted net was a study of the most frequent targets 5 years of single age, 4 years old, 3 years old, It was a three-order. Second, the research method consisted of the most experimental studies. Third, research topics include ecology toddler exploring related variables of education, ecological infant was the most common educational programs academic and theoretical basis for the direction sought, ecological Early Childhood Curriculum understanding and national levels of the education process, linking childcare courses, ecology teacher education the program was in order. Fourth, training in experiential learning is the most common forest garden gardening had appeared, walking activities, ecology, art, spirituality, work, raise animals and plants, environmental education in order.

• The Journal of the Korea Contents Association
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• v.13 no.9
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• pp.369-381
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• 2013

The purpose of the study was to divide social commerce websites' information into components of product, price, service, and seller information, and to systematically analyze each component of information. A content analysis was used as a research methodology, and 100 webpages from social commerce websites were analyzed. As results, the social commerce websites provided basic information (front view, fabric contents, country of origin, care, color, size) for product information and emphasized price information, including discount rates, sales dues, product quantity, and the amount of products sold. They basically provided expected delivery date, delivery company, Q&A, and return/refund service for service information. In addition, they basically presented brand logo and brand name but need to add information about brand concepts, history and volume as seller information. This study suggested diverse theoretical and practical implications of social commerce information.

• Food Science of Animal Resources
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• v.38 no.3
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• pp.636-652
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• 2018

The study aims to determine the optimum sterilization rate and water activity of Chinese traditional smoke-cured bacon product (Larou) in the preservation with natural coating solution. With the response surface methodology (RSM), we analyzed 3 factors of processing conditions (the concentration of lysozyme, concentration of sodium alginate, and concentration of chitosan) and 2 response factors (sterilization rate and water activity). Sterilization rate and water activity of Larou were largely affected by the concentration of lysozyme, concentration of sodium alginate, and concentration of chitosan. The final optimum concentrations of lysozyme, sodium alginate, and chitosan were 0.09, 1.40, and 1.60% and realized the high sterilization rate. Water activity of sliced Larou was significantly correlated with the sterilization rate. Low-field nuclear magnetic resonance analysis verified the optimum processing conditions. The coating resulted in 99.69% rate of reducing bacteria after 30-day storage. The data of the total number of colony, peroxidation value, moisture content, pH, and sensory evaluation provided the theoretical basis for extending the shelf life of Chinese traditional smoke-cured bacon product (Larou) with natural coating solution.

• The Journal of Korean Academic Society of Nursing Education
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• v.17 no.1
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• pp.5-13
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• 2011

Purpose: The purpose of this study was to develop a lesson plan related to a simulation for nursing students by understanding what relationship learning satisfaction, self-efficacy and learning attitude have after applying simulation-based education on emergency nursing care. Methods: Simulation practice, seminar, class and group self-study about a patient having a myocardial infarction were applied to nursing students for 5 weeks. After applying the simulation-based education on emergency nursing care, students rated their learning attitude, learning satisfaction and a sense of self-efficacy with a self-administered questionnaire and academic achievement was divided into written and performance evaluation. SPSS/WIN 17.0 was used for descriptive analysis, independent t-test, one-way ANOVA and pearson correlation were also used. Results: In this study, the performance evaluation score was related to learning attitude (r=.210, p=.018).Learning satisfaction (r=.220, p=.013)and written evaluation score was related to learning attitude (r=.211, p=.017) and learning satisfaction (r=.190, p=.032). Conclusion: In this study, simulation-based learning was a useful method for practical ability and this is good to acquire both of knowledge and technique. Not only evaluation of theoretical knowledge but performance ability related to practice has to be done by developing lessons with various methods and content. In addition, a standard evaluation method needs to be developed.

• Journal of Digital Contents Society
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• v.16 no.1
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• pp.145-155
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• 2015

Into the 1990s, there have been changes in perception of pop culture consumers by making a distinction between groups that are active, critical and creative. This study aims to establish a theoretical reasoning for conventions organized and participated voluntarily by fans, which has been especially noticeable in the American subculture for a long time, while investigating how fans and all the parties concerned interact at conventions then to expand the imaginary world. To this end, an analysis has been carried out on the conventions for the particular American TV series of Supernatural. The results show that the reason for this drama series maintaining such enduring vitality as if a living thing is attributable to the special bond of sympathy developed between fans and actors and the following interactions between them. Furthermore, in the aspect of cultural sociology, a piece of work can only be completed through unconstrained interpretation of the work and the resultant recreation by the creator and the receiver, which has been ascertained by this study.

• Journal of Digital Convergence
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• v.14 no.11
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• pp.549-555
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• 2016

This paper tries to find the implications applied to art institutions of Korea by analyzing what the content and consequences of services of the Cleveland Museum of Art provided after the Internet of Things adoption in the theoretical perspectives strengthening service marketing through the Internet of Things increases visits and participation in the museum. For analysis, journal articles, statistics, and government press releases, news articles, web pages, web page articles are collected. The results are first, the application of digital and social media is positive in visit to the museum and monetization. Second, the introduction of the Internet of Things will enhance the museum's 'Education', 'Accessibility', 'Communications' services, as well as significantly increase on and offline participation by activating experience. Third, technical support from related companies with the support of local government funds is essential to build the system. Consequently, in order to enable the participation of the spectators in the galleries it is proposed aggressive adoption of such digital technology in domestic barren market of Internet of Things.

• Journal of the Korean Chemical Society
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• v.62 no.2
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• pp.118-123
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• 2018

The hexagonal tungsten bronze $Cs_xWO_3$ nanoparticle was synthesized by a chemical coprecipitation method of ammonium tungstate and $Cs_2CO_3$ in acidic condition. This synthetic method for cesium tungsten bronze is reported for the first time as far as we know. The synthesized $Cs_xWO_3$ as precipitated showed a weak crystallinity of hexagonal unit cell with a crystallite size of about 4 nm without annealing. When the synthesized $Cs_xWO_3$ was annealed in $N_2$ atmosphere, the crystallinity and crystallite size systematically increased maintaining the typical hexagonal tungsten bronze structure as the annealing temperature increased. The analyzed Cs content in the bronze was about 0.3 vs W, which is very close to the theoretical maximum value, 1/3 in cesium tungsten bronze. According to XPS analysis, the reduced tungsten ions existed as both the forms of $W^{5+}$ and $W^{4+}$ and the contents systematically increased as the annealing temperature increased up to $800^{\circ}C$. The $Cs_xWO_3$ thin films on PET substrate were also prepared by a wet-coating method using the ball-milled solution of the annealed $Cs_xWO_3$ nanoparticles at various temperatures. The near-infrared shielding property of these thin films increased systematically as the annealing temperature increased up to $800^{\circ}C$ as expected with the increased contents of reduced tungsten ions.