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Cytokine-cytokine receptor interactions in the highly pathogenic avian influenza H5N1 virus-infected lungs of genetically disparate Ri chicken lines

  • Vu, Thi Hao;Hong, Yeojin;Truong, Anh Duc;Lee, Jiae;Lee, Sooyeon;Song, Ki-Duk;Cha, Jihye;Dang, Hoang Vu;Tran, Ha Thi Thanh;Lillehoj, Hyun S.;Hong, Yeong Ho
    • Animal Bioscience
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    • v.35 no.3
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    • pp.367-376
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    • 2022
  • Objective: The highly pathogenic avian influenza virus (HPAIV) is a threat to the poultry industry as well as the economy and remains a potential source of pandemic infection in humans. Antiviral genes are considered a potential factor for HPAIV resistance. Therefore, in this study, we investigated gene expression related to cytokine-cytokine receptor interactions by comparing resistant and susceptible Ri chicken lines for avian influenza virus infection. Methods: Ri chickens of resistant (Mx/A; BF2/B21) and susceptible (Mx/G; BF2/B13) lines were selected by genotyping the Mx dynamin like GTPase (Mx) and major histocompatibility complex class I antigen BF2 genes. These chickens were then infected with influenza A virus subtype H5N1, and their lung tissues were collected for RNA sequencing. Results: In total, 972 differentially expressed genes (DEGs) were observed between resistant and susceptible Ri chickens, according to the gene ontology and Kyoto encyclopedia of genes and genomes pathways. In particular, DEGs associated with cytokine-cytokine receptor interactions were most abundant. The expression levels of cytokines (interleukin-1β [IL-1β], IL-6, IL-8, and IL-18), chemokines (C-C Motif chemokine ligand 4 [CCL4] and CCL17), interferons (IFN-γ), and IFN-stimulated genes (Mx1, CCL19, 2'-5'-oligoadenylate synthase-like, and protein kinase R) were higher in H5N1-resistant chickens than in H5N1-susceptible chickens. Conclusion: Resistant chickens show stronger immune responses and antiviral activity (cytokines, chemokines, and IFN-stimulated genes) than those of susceptible chickens against HPAIV infection.

Occurrence characteristics and management plans of Paspalum distichum and P. distichum var. indutum (습지에서 발생하는 생태계교란야생식물인 물참새피와 털물참새피의 발생특성과 관리방안)

  • In Yong Lee;Seung Hwan Kim;Yong Ho Lee;Adhikari Pradeep;Dong Gun Kim;Sun Hee Hong
    • Korean Journal of Environmental Biology
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    • v.40 no.3
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    • pp.325-334
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    • 2022
  • Paspalum distichum and P. distichum var. indutum are perennial weeds of the family Poaceae that prefer moist environments such as waterfronts and waterways. The origin of both species is North America. P. distichum is distributed all over the world. However, P. distichum var. indutum occurs only in the United States, Japan, and Korea. For this reason, in many countries, P. distichum and P. distichum var. indutum are classified as the same species. In other words, P. distichum var. indutum is a different ecological type of P. distichum. Both species can reproduce and spread mainly by rhizome fragments rather than seeds. This rhizome has a characteristic that it does not germinate if it is buried in the ground with depth of more than 3 cm. As a management method for P. distichum and P. distichum var. indutum in agricultural lands (paddy fields), it is effective to combine cultural control and chemical control methods. In other words, combining deep plowing and harrowing can suppress the budding of water sparrow that has invaded paddy fields or fallow paddy fields. After that, these two species that germinate can be controlled by spraying soil treatment herbicides such as butachlor and thiobencarb or foliar treatment herbicides such as cyhalofop-butyl and fenoxaprop-p-ethyl.

Electronic Cigarette or Vaping Product Use-Associated Lung Injury: A Case Report (전자담배 관련 급성 폐 손상: 증례 보고)

  • Jiyun Lim;Bo Da Nam;Jung Hwa Hwang;Yang-Ki Kim;Eunsun Oh;Eun Ji Lee
    • Journal of the Korean Society of Radiology
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    • v.82 no.6
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    • pp.1581-1588
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    • 2021
  • Electronic cigarette (e-cigarette) or vaping product use-associated lung injury (EVALI) has emerged as a social issue as e-cigarette use is rapidly increasing worldwide and is related to many deaths in the United States. To our knowledge, this is the first case report of EVALI in South Korea of a 24-year-old man with acute respiratory symptoms and a history of e-cigarette use. Chest CT revealed diffuse bilateral ground-glass opacities with subpleural sparing, airspace consolidation, and centrilobular micronodules as typical patterns of EVALI with organizing pneumonia and diffuse alveolar damage. Infection was excluded with meticulous laboratory examinations, and the patients' illnesses were not attributed to other causes. EVALI was diagnosed by meeting the diagnostic criteria with consistent clinico-radiologic findings through a multidisciplinary approach. Radiologists should have good knowledge of EVALI radiologic findings and play a cardinal role in the proper diagnosis and management of EVALI.

Nationwide survey of Turnip mosaic virus and selection of cabbage lines with resistance against major TuMV isolates

  • Chung, Jinsoo;Han, Jae-yeong;Kim, Jungkyu;Ju, Hyekyoung;Gong, Junsu;Seo, Eun-young;Choi, Su Ryun;Lim, Yong Pyo;Hammond, John;Lim, Hyoun-Sub
    • Korean Journal of Agricultural Science
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    • v.43 no.4
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    • pp.567-574
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    • 2016
  • This survey was conducted in 2015, following up on theed tthe occurrence of Turnip mosaic virus (TuMV) nationwide in radish and Chinese cabbage fields of 28 cities in South Korea. A total of 152 samples of Raphanus sativus and 29 samples of Brassica rapa, showing virus-like symptoms, were collected. Among these, 107 B. rapa samples and 9 B. rapa samples were positive for TuMV when analyzed by RT-PCR. The TuMV strains found in the two crops showed 99% homology in nucleotide and amino acid sequences of coat protein to each other. Furthermore, their sequences showed 99% homology to the sequences of TuMV isolates R007 (GenBank: KU140420) and R041 (GenBank: KU140421) that were collected in 2014. These results suggested TuMV isolated from radish and cabbage in 2015 were the same strain as the isolates R007 and R041 collected in 2014. A screening test was conducted using these two isolates to select TuMV-resistant B. rapa lines out of 167 B. rapa breeding lines.and identified eight lines resistant to R007 (Kenshin, 279002, 279012, 279064, 279081, MP, C-21, HKC-004) and nine lines resistant to R041 (C-26, HKC-005, 11Su-4, 11Su-5, 11Su-7, 11Su-8, Tian Jin Lv Qing Ma Ye, CNU_141193, Jing Lv 60). Our prior data indicated 4.24% difference in sequences between the two isolates and these can serve as potential tools to develop B. rapa markers to screen for resistance against TuMV strainsin breeding populations.

Seasonal Dynamics of Enzymetic Activities and Functional Diversity in Soils under Different Organic Managements (시용 유기물을 달리한 토양에서 미생물 군락의 효소활성과 기능적 다양성의 계절적 변화)

  • Park, Kee-Choon;Kremer, Robert J.
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.4
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    • pp.307-316
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    • 2009
  • Soil microbial activity and diversity are affected by organic sources applied to improve soil quality and fluctuate seasonally. We investigated the effects of municipal compost (MC), poultry litter (PL), and cover crops of spring oats and red clover (RC) on soil enzyme activities, and soil bacterial community-level physiological profiling (CLPP) in a Mexico silt loam in North Central Missouri, USA. Temporal patterns of these parameters were observed by periodic five soil sampling from spring to fall over a two year period. MC increased soil dehydrogenase (DH) activity consistently beginning about three months after MC application; fluorescein diacetate (FDA) hydrolytic activity significantly began to increase by the September of the first year but fluctuated during the following period. DH activity responded more directly to the amount or properties of organic residues in soils while FDA hydrolysis and CLPP were generally influenced by composition of organic sources, and enzyme activities and CLPP showed seasonal variation, which depended on organic sources and soil moisture. MC and cover crops may be useful organic sources for enhancing general soil microbial activity and altering soil microbial diversity, respectively. Because microbial activities and diversity are dynamic and subject to seasonal changes, the effects of organic amendments on these parameters should be investigated frequently during a growing season.

Wide Area Augmentation System Estimating C1P1 DCB (C1P1 DCB를 추정하는 광역보강항법 시스템)

  • Bu, Sung-Chun;So, Hyoung-Min;Kim, Kap-Jin;Lee, Chul-Soo;Kim, Do-Kyoung;Ko, Yo-Han
    • Journal of Advanced Navigation Technology
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    • v.22 no.5
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    • pp.400-408
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    • 2018
  • Wide area augmentation system is a system that generates and transmits correction and Integrity information for use in wide area. Typical system is SBAS. In the United States, it operates under the name WAAS, EGNOS in Europe, MSAS in Japan, SDCM in Russia, GAGAN in India. it is developing Korean SBAS which named KASS by 2022 in Korea. SBAS is a standard System that is operated as civil aviation service base and set as international standards by ICAO. So the correction data can only is used for civil SPS receiver. In this paper, we discuss C1P1 DCB estimation which need to use SPS correction service for PPS receiver. Then we analyze C1P1 DCB correction effect under standalone Satellite Navigation and method to use PPS receiver under SPS DGPS. Finally we organize wide area augmentation system for PPS receiver and analysis performance.

Advance Care Planning: Preliminary Report of Differences and Similarities between Korean and Korean American

  • Park, Jin Hee
    • Journal of Hospice and Palliative Care
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    • v.16 no.4
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    • pp.232-241
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    • 2013
  • Purpose: This study was conducted to do preliminary report of differences and similarities between Koreans residing in Korea and Korean Americans residing in America regarding their awareness of end-of-life care, attitudes toward advance care planning, truth telling, and preferred decision-making model. Methods: Two participating groups were selected: a) Koreans residing in Korea, and b) Koreans Americans who had resided in the United States for at least 20 years. 25 Koreans and 23 Korean Americans who were older than 65 years old participated in this study. They were asked via a self-administered questionnaire that contained demographic questions and questions about end-of-life decision making regarding awareness of end-of-life care, attitudes toward advance care planning, truth telling, and preferred decision-making model. A Chi-square was used to measure differences between Koreans' and Korean Americans planning. A P value of less than 0.5 was considered significant. Data analysis was performed using SPSS 18.0. Results: In some aspects of awareness of end of life care, attitudes toward advance care planning, and truth telling, both groups had similar opinions. However, there were significant differences between groups in the necessity of end of life documentation, preferential informing the truth, and preferred decision making model. Conclusion: There were similarities and differences regarding some end of life issues between the Koreans and the Korean Americans.

Silage Productivity of Korean Improved and Introduced Maize Hybrids (국내 육성 및 수입 옥수수 품종의 사일리지 생산성)

  • Lee, S.S.;Yun, S.H.;Seo, J.M.;Yang, S.K.;Min, H.K.;Ryu, S.H.;Park, J.Y;Kim, S.K.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.24 no.4
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    • pp.323-334
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    • 2004
  • Silage yield Potential and some agronomic characteristics of Korean improved and introduced corn hybrids from the United States were tested for five year in Gyeongsan, Gyeongsangbug-do and for one year in Hongcheon, Gangwon-do. 1. At 20 days after emergence, plant height and dry matter of hybrids were different, while the early growth of the hybrids was not correlated to the culm length and silage yield. 2. In Cyeongsan, silage yield potential of Suwon19 was relatively high, while most susceptible to rice black-streaked dwarf virus (RBSDV) disease and leaf senescence at harvest time. In contrast, silage yield potential and resistance to RBSDV of Cwanganok were moderate, while susceptible to leaf senescence at harvest. Generally, Suwon19 showed similar or higher yield than most introduced hybrids, while some introduced hybrids showed lower silage yield than Korean improved hybrids. Most introduced hybrids were more resistant to RBSDV and leaf senescence at harvest time compared to Korean improved hybrids. 3. In Hongcheon, silage yield of three Korean improved hybrids were lower than that of NC+5514 and DK729, while similar or higher than other introduced hybrids. af senescence of all Korean hybrids was severer compared to introduced hybrids.

Silibinin Inhibits Cell Growth and Induces Apoptosis through Cell-cycle Arrest in PC-3 Prostate Cancer Cells (인간 전립선 암세포 PC-3 세포에서 Silibinin의 세포주기조절을 통한 세포사멸 유도 효과)

  • Kim, Sang-Hun;Kim, Kwang-Youn;Yu, Sun-Nyoung;Jeon, Hyun-Joo;Jin, Young-Rang;Lee, Chang-Min;Ahn, Soon-Cheol
    • Journal of Life Science
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    • v.21 no.11
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    • pp.1573-1578
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    • 2011
  • Milk thistle (silybum marianum) is a famous dietary supplement widely used in the United States and Europe. Silbinin is a major biologically active compound of milk thistle and has strong antioxidant and radical scavenger activities. Anticancer activities, as well as chemopreventive effects on various cancer cell lines, including prostate, lung, colon, skin, and bladder, have also been reported in silbinin. In the present study, we investigated the anticancer effects of silibinin and apoptosis through cell cycle arrest on prostate cancer cell PC-3. We performed cell viability by MTT assay and western blotting to confirm cell cycle check point proteins such as cyclin A/D1/E and cyclin-dependent kinase (CDK) 2/4/6. To quantify silibinin-induced apoptotic cell death of PC-3, Annexin V and PI double staining was performed by flow cytometry, by which its cell distribution was determined. As a result, silibinin inhibited the cell growth of PC-3 cells in a time- and dose-dependent manner, and its treatment resulted in cell cycle arrest at the G1 phase. Also the level of cell cycle check point proteins (cyclin, CDK) was decreased by silibinin in a dose-dependent manner. Taken together, we suggest that apoptosis of prostate cancer cell line PC-3 induced by silibinin is associated with cell cycle arrest through decrease of cell cycle check point proteins, caspase-3 activation and poly (ADP-ribose) polymerase (PARP) cleavage.

Case Analysis Study of Global Femvertising Campaign for Female Empowerment (여성들의 권익신장을 위한 글로벌 펨버타이징 광고 캠페인 사례분석)

  • Um, Namhyun
    • Journal of Digital Convergence
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    • v.18 no.7
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    • pp.389-395
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    • 2020
  • Female empowerment advertising campaigns are easily observed in the United States and European countries whereas it is rarely found in Korea. Thus, this study is designed to analyze female empowerment advertising campaigns so-called femvertising through a case analysis method. The case analysis is often employed in the field of advertising and other social sciences, especially is helpful in finding current trends. Study results found that themes of global femvertising campaigns could be categorized into 'redefining women's beauty', 'gender equality', 'education', and 'leadership'. The objective of these femvertising campaigns is to change perspectives of the society on women and change men's perspective. In terms of product category by global femvertising campaign companies, it varies from consumer goods, IT & electronics, automotive, finance, toy, non-profit organization to press. In general, femvertising targets women, but some femvertising campaigns also target men. This study also provides implications in regards with media usage strategy and advertising planning strategy.