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Investigation of ${\beta}$-Lactamase-producing Multidrug-resistant Pseudomonas aeruginosa Isolated from Non-Tertiary Care Hospitals in Korea

  • Sohn, Eui-Suk;Yoo, Jeong-Sik;Lee, Jeom-Kyu;Lee, Kyeong-Min;Chung, Gyung-Tae;Shin, Eun-Shim;Han, Sun-Young;Lee, Sang-Hee;Kim, Joon;Lee, Yeong-Seon
    • Journal of Microbiology and Biotechnology
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    • v.17 no.10
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    • pp.1733-1737
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    • 2007
  • A total of 2,280 nonduplicate clinical isolates of Pseudomonas aeruginosa, obtained nationwide from Korean non-tertiary care hospitals from 2002 to 2005, were identified and their susceptibilities to aminoglycosides, antipseudomonal penicillins, carbapenems, cephalosporins, monobactams, and quinolones were studied, together with their production of ${\beta}$-lactamases. Using disk diffusion and minimum inhibitory concentration tests, it was found that 2.9% of isolates were multidrug-resistant (MDR) P. aeruginosa. An EDTA-disk synergy test, PCR amplification with specifically designed primers, and direct sequencing of the PCR products showed that the $bla_{OXA-10}$, $bla_{VIM-2}$, $bla_{OXA-2}$, $bla_{OXA-17}$, $bla_{PER-1}$, $bla_{SHV-12}$, and $bla_{IMP-1}$ genes were carried by 34.3%, 26.9%, 3.0%,3.0%, 1.5%, 1.5%, and 1.5% of 67 MDR P. aeruginosa isolates, respectively. The prevalence of MDR P. aeruginosa was three-fold higher, compared with that from the United States. More than two types of ${\beta}$-lactamase genes were carried by 10.4% of isolates. The most prevalent ${\beta}$-lactamase genes were $bla_{VIM-2}$ and $bla_{OXA-10}$. This study is the first description of MDR P. aeruginosa trom non-tertiary care hospitals in Korea and the coexistence of the $bla_{VIM-2}$, $bla_{IMP-1}$, or $bla_{PER-1} in these clinical isolates.

A Study on Detection of Residual Solvent, Ethoxyquin and Color Stability in Oleoresin Paprika Extracts (파프리카 추출물의 색소안정성과 Ethoxyquin 및 잔류용매 검출)

  • Lee, Seon-Ok;Kyung, Suk-Hun;Park, Kil-Dong;Kang, Hee-Gon;Park, Joo-Sung;Lee, See-Kyung
    • Applied Biological Chemistry
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    • v.45 no.2
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    • pp.77-83
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    • 2002
  • Effects of ethoxyquin on the color stability of oleoresin paprika extracts and amount of residual ethoxyquin, a color stabilizer, in commercial extracts were determined. The oleoresin paprika extracts dissolved in ethanol gave the highest maximum photo-absorbency at 444-458 nm, with the color index of United States product 2-6 times higher than that produced in India. The residual solvents in oleoresin paprika extracts were mainly acetone and methanol, although some other extracts also contained small amounts of hexane. HPLC analysis was determined as a proper analytical method for residual ethoxyquin assay in the oleoresin paprika extracts, particularly when hexane was used as a solvent. The residual ethoxyquins were detected in the extracts produced in US and Spain which had relatively high color indices.

A Study on Development of GenBank-based Prototype System for Linking Heterogeneous Content (GenBank를 활용한 이종의 콘텐트 연계 프로토타입 시스템 개발 연구)

  • Ahn, Bu-Young;Shin, Young-Ju;Kim, Dea-Hwan
    • Journal of Information Management
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    • v.40 no.4
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    • pp.109-133
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    • 2009
  • Among biological information, GenBank, provided by the National Center for Biotechnology Information (NCBI)of the United States, is a representative database on genetic information and is the most widely used by researchers around the world. Korea Institute of Science and Technology Information (KISTI) visits NCBI on a regular basis and downloads the latest version of GenBank to reorganize the information gathered there into a database. This database is provided for Korean researchers of science and technology through the Bio-KRISTAL search engine, developed by KISTI. This study aims to design a service model that links information on papers, patents, and biodiversity and other contents of NDSL, an integrated service on scientific and technological information run by KISTI, with GenBank's reference and organism fields and to develop a prototype system. For this purpose, this paper explores the possibility of a linkage and convergence service between heterogeneous content by: (a) collecting GenBank data from NCBI's FTP site; (b) dividing GenBank text files into basic and reference genetic information and restructuring them into a database; (c) extracting article and patent information from the GenBank reference fields to generate new tables; and (d) leveraging data mapping technology to implement a prototype system where GenBank and NDSL data are interlinked and provided.

Discrimination of cows from heifers by antemortem and postmortem inspection in slaughter house (도축장의 생.해체검사를 통한 경산우 및 미경산우 감별법(I))

  • Choi, Im-Yong;Choi, Tae-Suk;Lee, Dal-Ju;Lee, Hae-Yeong;Rho, Chang-Sik;Kim, Kyu-Hiun;Kim, Eun;Kweon, Taek-Boo;Lee, Jung-Hark
    • Korean Journal of Veterinary Service
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    • v.30 no.3
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    • pp.429-436
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    • 2007
  • This study was performed to make helpful standard for discrimination of cows from heifers by uterine and ovarian section inspection. A total of 3,736 heifers and 249 cows were inspected from January to November 2006 in Karak Market Slaughter house. Firstly, it was obvious that non-corpus albicans were observed in almost heifers(3,597/3,736, 96.3%), but there were distinctive corpus albicans in all cows (249/249 100%). Secondly, another evident was that the length and diameter of the horn in uterus was below 30mm in 94.5% (3,267/3,736) heifers, but above 30mm in 90.4% (225/245) cows, and it was easy to distinguish between right and left horn by difference of size in 87.6% (218/249) cows. Thirdly the diameters of pelvic cavity was below 220mm in 96.8% (3,615/3,736) heifers, but was over 200mm in 85.1% (212/249) cows. Fourthly another difference was found in second sacrum union condition and 13th thoracic vertebral process's states. In case of heifers, it was distinctive five lines in each second sacrum, but it was non-distinctive or united in the same each lines of the sacrum in cows. Fifthly in the results of 13th thoracic vertebral colors, the ratio between red color and white color was 7 : 3 in heifers (3,385/3,736, 90.6%), but that was 2 : 8 in cows (199/249, 79.9%). Consequently, it was possible to use the method which discriminated cows from heifers by uterine and ovarian section inspection in large scaled field slaughter house.

Study on Innovation Measurement of National R&D Investments for Nanotechnology Using Data Envelopment Analysis (자료포락분석을 통한 국가 나노기술 연구개발투자 결과의 혁신성 분석 연구)

  • Lim, Jung Sun;Hahn, Hyuk;Won, Dong-Kyu;Kim, Sanggook
    • Journal of Korea Technology Innovation Society
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    • v.22 no.2
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    • pp.207-219
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    • 2019
  • The international trends in activities of government R&D performance measurement is evolving into evidence-based approach to support the verification of objective policy implementation. The European Commission has been implementing KETs (Key Enabling Technologies) policy that incubates nanotech based emerging technologies to support the fourth industrial revolution/revitalization of high-tech manufacturing, and resulting innovations are quantified by KETs Observatory project. The European Commission also built Innovation Radar system that monitors potentially innovative projects within FP7 and Horizon2020 by data analysis. The United States is also upgrading its Federal RePORTER system to quantitatively monitor federal R&D investments with outcomes (including nanotechnology). In the field of Korean nanotechnology, basic statistical data by analyzing NTIS (National Science & Technology Information Service) information is applied in policy field. Developing innovation measurement methodology beyond basic statistical analysis is an international policy issue, and a long-term R&D investment area of a government. The objective of this model study is to quantify the innovation potential of nano R&D investments conducted by Korea government, using input-output based efficiency measurement model and NTIS (National Science & Technology Information Service) that is comprehensive data portal for national R&D investments/outcomes including nanotechnology.

Analysis of Minimum Logistics Cost in SMEs using Korean-type CIPs Payment System (한국형 CIPs 결제 시스템을 이용한 중소기업의 최소 물류비용 분석)

  • Kim, Ilgoun;Jeong, Jongpil
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.21 no.1
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    • pp.7-18
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    • 2021
  • Recently, various connected industrial parks (CIPs) architectures using new technologies such as cloud computing, CPS, big data, fifth-generation mobile communication 5G, IIoT, VR-AR, and ventilation transportation AI algorithms have been proposed in Korea. Korea's small and medium-sized enterprises do not have the upper hand in technological competitiveness than overseas advanced countries such as the United States, Europe and Japan. For this reason, Korea's small and medium-sized enterprises have to invest a lot of money in technology research and development. As a latecomer, Korean SMEs need to improve their profitability in order to find sustainable growth potential. Financially, it is most efficient for small and medium-sized Korean companies to cut costs to increase their profitability. This paper made profitability improvement by reducing costs for small and medium-sized enterprises located in CIPs in Korea a major task. VJP (Vehicle Action Program) was noted as a way to reduce costs for small and medium-sized enterprises located in CIPs in Korea. The method of achieving minimum logistics costs for small businesses through the Korean CIPs payment system was analyzed. The details of the new Korean CIPs payment system were largely divided into four types: "Business", "Data", "Technique", and "Finance". Cost Benefit Analysis (CBA) was used as a performance analysis method for CIPs payment systems.

Analysis of Patent Trends in Agricultural Machinery (최신 농업기계 특허 동향 조사)

  • Hong, S.J.;Kim, D.E.;Kang, D.H.;Kim, J.J.;Kang, J.G.;Lee, K.H.;Mo, C.Y.;Ryu, D.K.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.23 no.2
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    • pp.99-111
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    • 2021
  • The connected farm that agricultural land, agricultural machinery and farmer are connected with an IoT gateway is in the commercialization stage. That has increased productivity, efficiency and profitability by intimate information exchange among those. In order to develop the educational program of intelligent agricultural machinery and the agricultural machinery safety education performance indicator, this study analyzed patent trends of agricultural machine with unmanned technology used in agriculture and efficiency technology applied advanced technologies such as ICT, robots and artificial intelligence. We investigated and analyzed patent trends in agricultural machinery of Korea, the USA and Japan as well as the countries in Europe. The United States is an advanced country in the field of unmanned technology and efficiency technology used in agriculture. Agricultural automation technology in Korea is insufficient compared to developed countries, which means rapid technological development is needed. In the sub-fields of field automation technology, path generation and following technology and working machine control technology through environmental awareness have activated.

Research Trends of Foreign Countries on Geological Evaluation of Abiotic Hydrogen Productivity: A Review (비생물기원 수소 생산성의 지질학적 평가 관련 해외 연구 동향: 리뷰 논문)

  • Jeong, Seongwoo;Kim, Taeyong;Ko, Kyoungtae;Yang, Minjune
    • The Journal of Engineering Geology
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    • v.32 no.4
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    • pp.627-642
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    • 2022
  • The world's long reliance on fossil fuels (e.g., oil, coal, and natural gas) is severely changing its environment and climate. Energy research has focused on developing hydrogen as the most promising energy carrier and a key technology for sustainable energy development. Hydrogen can be classified as gray, blue, green, and otherwise according to the raw materials and methods used for production and processing. For the development of hydrogen energy, geologists are attempting to identify the mechanism of abiotic hydrogen generation by serpentinization or hydrothermal alteration. Teams in the United States, France, and Australia have researched laboratory-scale hydrogen production through water-rock interactions under various conditions, whereas there has been almost no research on abiotic hydrogen in South Korea. This paper reviews the current state of international research on hydrothermal alteration and offers suggestions for future investigations of abiotic hydrogen production in South Korea.

Screening for Genotypes Lacking Lipoxygenase from Germplasm Collection of Korean Soybean Land Races (한국 재래종 콩집단에서 비린내 없는 콩품종 육성을 위한 Lipoxygenase 결실인자 변이 연구)

  • Kwon, Shin-Han;Park, Kyung-Sook;Kim, Mi-Young;Kim, Bong-Ryong;Song, Hi-Sup
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.6
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    • pp.528-533
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    • 1992
  • Soybean seeds contain lipoxygenase, which is responsible for the objectionable beany flavors in soybean seeds. The isozymes of lipoxygenase (1$\times$1, 1$\times$2, 1$\times$3) were discovered in United States of America, Japan, and Korea, and the mode of inheritance of the mutant genes was determined. This investigation was conducted to screen lipoxygenase-1, 2, and 3 lacking soybean lines from the Korean soybean land race population. Two lipoxygenase-1lacking lines, KAS 610-8 and KAS 621-8 were found in this investigation. In general, lipoxygenase acking varieties were small in seed size and low in oil content. A severe pod borer damage was observed in the two selected lipoxygenase-1 lacking lines. Lipoxygenase lacking line was not found in Korean wild soybean population used in this study and the lipoxygenase lacking lines were found only in Kyung-Nam province and the results imply that lipoxygenase lacking mutants were induced recently in cultivars.

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Trends in truffle mushroom utilization technology: A patent database survey through the first half of 2023 (트러플 활용기술 동향: 2023년 상반기까지의 특허를 중심으로)

  • Hyun-Suk Kim;Moon-Hee Choi;Deuk-Sil Oh;Hyun-Jae Shin
    • Journal of Mushroom
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    • v.21 no.3
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    • pp.88-92
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    • 2023
  • In this review, we examine the latest technological developments in the utilization of truffles, a gourmet ingredient reputed to be one of the "world's three greatest delicacies," considering changing global consumption trends. Global demand for truffles is expected to increase steadily, with an average annual growth rate of 8.9% from 2023 to 2030. As truffles are expensive, the demand for truffles is expected to be concentrated in developed countries such as the United States, European countries, and Japan. In Korea, truffles are utilized in various industries, including food, functional foods, and cosmetics. Korean consumer demand for truffles has consistently remained high since 2019, and truffle products have been performing well in the market. Consequently, there exists substantial potential demand for newly developed truffle-related products and technologies. This review aims to provide objective research information through the systematic analysis of patent applications in Korea and internationally, focusing on technologies involving truffles, and can aid in setting directions for research and development.