Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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A Study on Detection of Residual Solvent, Ethoxyquin and Color Stability in Oleoresin Paprika Extracts

파프리카 추출물의 색소안정성과 Ethoxyquin 및 잔류용매 검출

  • Lee, Seon-Ok (Dept. of Food & Drug Analysis, Food & Drug Administration Seoul Regional Office) ;
  • Kyung, Suk-Hun (Dept. of Applied Biol. and Chem., Kon-Kuk University) ;
  • Park, Kil-Dong (Dept. of Food Industry Korea Health Industry Development Institute) ;
  • Kang, Hee-Gon (Seoul Metropolitan Government Institute of Health & Environment) ;
  • Park, Joo-Sung (Seoul Metropolitan Government Institute of Health & Environment) ;
  • Lee, See-Kyung (Dept. of Applied Biol. and Chem., Kon-Kuk University)
  • 이선옥 (서울지방식품의약품안전청) ;
  • 경석헌 (건국대학교 응용생물화학과) ;
  • 박길동 (한국보건산업진흥원) ;
  • 강희곤 (서울시 보건환경연구원 가락동 농수산물 검사소) ;
  • 박주성 (서울시 보건환경연구원 가락동 농수산물 검사소) ;
  • 이시경 (건국대학교 응용생물화학과)
  • Published : 2002.05.31
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Abstract

Effects of ethoxyquin on the color stability of oleoresin paprika extracts and amount of residual ethoxyquin, a color stabilizer, in commercial extracts were determined. The oleoresin paprika extracts dissolved in ethanol gave the highest maximum photo-absorbency at 444-458 nm, with the color index of United States product 2-6 times higher than that produced in India. The residual solvents in oleoresin paprika extracts were mainly acetone and methanol, although some other extracts also contained small amounts of hexane. HPLC analysis was determined as a proper analytical method for residual ethoxyquin assay in the oleoresin paprika extracts, particularly when hexane was used as a solvent. The residual ethoxyquins were detected in the extracts produced in US and Spain which had relatively high color indices.

올레오레진 파프리카의 색소 안정성과 ethoxyquin의 잔류량 분석을 위하여 용매별 최대 흡수파장과 흡광도, 용매와 ethoxyquin파의 잔류량 분석 조건을 비교 분석한 결과는 다음과 같다. 용매별 올레오레진 파프리카의 최대 흡수파장은 444-458 nm에서 나타났으며 흡광도 값은 에탄올에서 가장 높게 나타났고 아세톤, 클로르포름, 메탄올의 순으로 높게 나타났다. 용매별 올레오레진 파프리카 추출물에서의 색가는 에탄올, 아세톤, 클로르포름 그리고 메탄올 순으로 높았으며 국가별로는 미국 제품이 용매에 따라서 다른 나라의 제품보다 2-6배 정도가 높았다. 올레오레진 파프리카에서 잔류되는 용매는 주로 아세톤, 메탄올 그리고 헥산이 일부제품에서 미량 검출되었으며 기타의 잔류용매는 검출되지 않았다 Ethoxyquin의 분석에서 GC에 의한 분석방법으로는 다양한 분석조건에서 실시하였으나 적합하지 않았다. HPLC에 의한 ethoxyquin의 분석을 위한 올레오레진 파프리카 추출물의 용해용매로 헥산을 이용한 것이 가장 분리능이 좋았으며 검출한계는 $0.01\;mgl^{-1}$이었다. 생산국별 올레오레진 파프리카에서의 ethoxyquin 검출은 미국과 스페인 제품에서 미량 검출되었으며 ethoxyquin이 검출된 제품이 검출되지 않은 제품보다 색가가 더 높게 나타났다.

Keywords

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