• Clinical and Experimental Reproductive Medicine
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• v.24 no.3
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• pp.347-353
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• 1997

This study was to test whether in vitro/in vivo survival of vitrified mouse blastocysts was influenced by culture conditions and ET method. Mouse blastocysts were obtained from in vitro fertilization and cultured for 4 days in M16 medium, and they were vitrified in EFS40 which contained 40% ethlyene glycol, 18% Ficoll and 0.5 mol sucrose in PBS. In experiment I, in vitro and in vivo survival rate of these embryos were evaluated in different culture condition after thawing. When thawed embryos were cultured in M16 medium as a control, m-CR1 medium contained 20 amino acids (2% BME amino acis and 1% MEM non-essential amino acids solution) and 4 mg/ml BSA and cumulus monolayer cell co-cultured condition in mCR1 medium (10% FBS), their in vitro survival at 24 hr after thawing was not affected by culture condition (75.6, 83.1, 82.4%). However, in vivo survival rates of implantation in m-CR1 medium (80.4%) were significantly higher than those of M16 medium (51.2%), co-culture (57.1%) condition, although there was no difference in live fetuses rates on day 15 gestation (39.0, 49.0, 38.1%). In experiment II, the in vivo development potential of embryos by ET methods was examined. When blastocysts were transferred to the day 2, 3 pseudopregnant recipient without culture soon after thawing, no pregnant recipient was obtained on the day 2 pseudopregnancy, and 50% of pregnancy rates and 15.4% of live fetus rates were obtained on the day 3 pseudopregnant recipients. These results were significantly lower than those of transferred group (day 3 pseudopregnant recipients) after culture for 16 hr post thawing (73.5, 57.1%) (p<0.05). In experiment III, to elevate usability of delayed embryos in vitro/in vivo survival of vitrified embryos (day 4 early, day 5 early and expanding blastocyst) were examined. in vivo survival rates (live fetus, total implantation) were higher in day 4 early blastocysts (33.3, 66.7%) than in day 5 expanding blastocysts (29.0, 38.7%), although the highest in vitro survival rates were obtained in the day 5 expanding brastocysts (78.3%). Therefore, these results suggest that the in vitro/in vivo survival rates of vitrified embryos could be improve by the culture condition and ET method and that the in vivo development rates of delayed embryos were decreased with longer culture duration in vitro. It means that more effective cryopreservation was obtained in day 4 early blastocysts than in day 5 expanding blastocysts.

• Clinical and Experimental Reproductive Medicine
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• v.34 no.2
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• pp.117-124
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• 2007

Objective: The aim of this study was to compare the efficacy of slow freezing with vitrification method for cryopreservation of mouse pronuclear stage embryos. Methods: Mouse pronuclear embryos obtained from superovulated mice and classified into 2 groups of slow freezing and vitrification. Slow freezing solution consisted of 1.5 M PROH, 0.1 M sucrose, while vitrification solution consisted of 40% ethylene glycol, 18% Ficoll and 0.5 M sucrose diluted in Dulbecco's phosphate-buffered saline supplemented with 10% SSS. Recovery and survival rates after thawing and development rates to hatching balstocyst stage were compared between two groups. Results: After freezing and thawing, recovery rate of slow freezing group was 93.8%, whereas vitrification group was 66.5% (p<0.01). Survival rate of recovered embryos were similar between two groups as 83.2% in slow freezing and 87.6% in vitrification. Embryo development rates to 2-cell stage after 24 hrs (77.0% vs 59.1%), 4-cell after 48 hrs (72.6% vs 53.3%), blastocyst after 96 hrs (53.1% vs 40.1%) of thawing were significantly higher in vitrification group than those of slow freezing group, respectively. Conclusion: The vitrification method may provide better developmental competence of frozen-thawed embryos than that of slow freezing method for cryopreservation of mouse pronuclear stage embryos.

• Korean Journal of Animal Reproduction
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• v.15 no.3
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• pp.173-177
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• 1991

In vitro survival of the mouse morulae frozen by vitrification method(Kasai et al., 1990) was investigated in the present study. The embryos were plunged into LN2 directly after exposure to the vitrification solutions(EFS, GFS and DFS). The results were obtained as follows. The viability of morulae after freezing and thawing was high in EFS(96.7∼100.0%) and GFS vitrification solution(93.3∼96.7%), and the lowest in DFS vitrification solution(0.00∼0.03%).

• Proceedings of the Korean Geotechical Society Conference
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• 1995.06b
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• pp.88-116
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• 1995

The applicability of marine clay and banking material as landfill liner and supporting layer was examined, where additives (cement, lime, Mg0, bentonite and ESCA) were mixed with these soils. The mixtures were tested for the unconfined compressive strength, bending strength, permeability, and better results than existing solidifying agents were obtained. The freezing-thawing test is under way.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.300.2-300.2
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• 2003

Unlike cationic liposome/DNA complexes, neutralliposomes containing plasmid DNA are stable in blood and does not selectively entrapped in the lung. The objective of this study was to construct neutral liposomes containing plasmid DNA with optimal encapsulation efficiency. Plasmid DNA (pGL2 clone 753,-6 kb) was encapsulated by the freeze/thawing method into liposomes composed of 1-palmitoyl-2-oleyl-sn-glycerol-3-phosphocholine(POPC), didodecyldimethylammonium bromide(DDAB), distaroylphosphatidyl-ethanolamine polyethylene glycol 2000(DSPE-PEG 2000) and DSPE-PEG 2000-maleimide. (omitted)

• Korean Journal of Animal Reproduction
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• v.17 no.4
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• pp.311-323
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• 1994

This study was conducted to observe seasonal and individual changes in semen characteristics and sperm freezability, and sperm penetration into zona-free hamster eggs in Korean native goats. Buck response and change in semen characteristics to electrical stimulations was evaluated for four seasons throughout 2 years and percentage of motile sperm and normal apical ridge acrosome was investigated after equilibration and thawing for 4 seasons with 5 bucks. Sperm penetration rate was evaluated for 4 bucks. 1. Probe insertion at depth of 7cm and repeated stimulation for 3 sec was more effective(P<0.05) in buck response and semen collection than those of other conditions. 2. Semen characteristics from electrojaculation was signficantly(P<0.005) higher in spring and fall for semen volume, in spring and summer for sperm concentration and in fall for sperm motility than those in other seasons, respectively. However, there were no differences in total sperm among seasons. 3. Buck response to electrical stimulation showed significant difference(P<0.05) among individuals in all 3 seasons except winter. Significant individual difference in semen volume was only in spring and summer, but there was no indivudual difference in sperm concentration and total sperm in all season. 4. Washing of semen before freezing treatment was greatly(P<0.05) beneficial to sperm motility after thawing, no matter whether ejaculates exhibit egg yolk coagulation or not. 5. Sperm motility after glycerol equilibration was significantly(P<0.05) low in summer semen and motility after thawing was greatly(P<0.05) higher in winter semen than in other seasons. Freezability of unwashed sperm was significantly difference among bucks, but a yearly freezability of washed sperm after chilling and thawing were no differences among bucks and percentage of normal apical ridge acrosome were not different among seasons and bucks. 6. There was no significant difference in sperm motility after thawing between egg yolk levels in summer, although 20% level gave more higher motility than 5% level. 7. In summer, 3.2% glycerol and 3-h equilibration gave greatest percentage(P<0.05) of sperm motility and normal apical ridge acrosome after thawing. 8. Sperm penetration rate into zona-free hamster eggs was not different between bucks and seasons. Overall, it is concluded that to obtain maximum sperm output and successive semen freezing by electrojaculation method, buck selection with good response in all season could be basically considered and that seasonal effect on sperm freezability was more greater than that of individual bucks.

• Proceedings of the Korea Concrete Institute Conference
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• 2006.05b
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• pp.309-312
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• 2006

In this study, the repair method using PVA fiber reinforced mortar evaluated on durability performance. Test items are compressive strength, flexural strength, carbonation, freezing-thawing. As for the test results, it was found that durability performance of the repair method using PVA fiber mortar showed more better than the existing repair method. Therefore, appling on the repair method using PVA fiber mortar, the repaired concrete structures can be increased to service life.

• Journal of the Korean GEO-environmental Society
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• v.17 no.6
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• pp.23-31
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• 2016

Nowadays abnormal coldness happens frequently in Korea and frost heaving causes unexpected ground deformation which results in severe problems for structures such as roadway, railroad and cutoff slope. 'Frost heave' as one of the primary phenomenon is considered to be an important factor together with 'adfreeze bond-strength' and 'creep deformation' for structural design process in permafrost area. Therefore, the fundamental study for frost heave has to be preceded for design of geo-structures in cold region. While various experimental apparatuses have been developed, there still exist a certain level of limitation to evaluate the frost-heave characteristics as design parameters. There are no standard testing method and criteria for analyzing frost heaving in Korea because temperature controlled testing apparatuses including a freezing chamber are expensive. In this paper, a new standard freezing and thawing testing apparatus is introduced, which simulates various freezing and thawing conditions in a soil specimen by using a temperature controlled triaxial cell. Frost heaving tests were performed to assess the new testing apparatus and experimental procedure to evaluate frost heaving for soils is proposed.

• Journal of Industrial Technology
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• v.24 no.A
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• pp.157-164
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• 2004

Air voids in hardened concrete have an important influence on concrete durability such as resistance of freezing and thawing, permeability and surface scaling resistance. Linear traverse method and point count method in ASTM standard method have been widely used to estimate the air void system in hardened concrete. However, these methods are not used at present time, because they are is exhausted much time and effort. In previous study, air voids system of concrete was estimated by spacing factor. The purpose of this study organizes image analysis method by analyzing air contents, air voids distributions by diameters, air voids system as well as spacing factors after hardened concrete. The experimental variables institute of depth of specimen(top, middle, bottom), air contents(AE contents 0, 0.01, 0.03%).

• Proceedings of the Korea Concrete Institute Conference
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• 2006.11a
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• pp.621-624
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• 2006

In this study, the repair method using PVA fiber reinforced mortar evaluated on durability performance. Test of salt injury, carbonation, freezing-thawing, chemical attack, permeability was performed As for the test results, it was found that durability performance of the repair method using PVA fiber mortar showed more better than the existing repair method. Therefore, appling on the repair method using PVA fiber reinforced mortar, the repaired concrete structures can be increased to service life.