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Effects of the Fructooligosaccharide Intake on Human Fecal Microflora and Fecal Properties (Fructooligosaccharide의 섭취에 의한 인체의 장내세균(腸內細菌) 및 분변(糞便) 성상의 변화)

  • Kang, Kook-Hee;Kim, Kyung-Min;Choi, Sun-Gyu
    • Korean Journal of Food Science and Technology
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    • v.28 no.4
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    • pp.609-615
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    • 1996
  • To study the effects of the fructooligosaccharide (FOS) intake on the intestinal microflora, the FOS (8g) was served to each of 5 volunteers (adult men $23{\sim}28$ years old) after every lunch for 4 weeks. Changes in fecal microflora, fecal moisture, and fecal pH were observed during the FOS intake and after the FOS intake, respectively. The fecal moisture content of the control period (4 weeks before the intake of FOS) was $81.77{\pm}1.18%$. The moisture content of the feces increased significantly at the end of the 4th week of FOS intake, and these effects lasted for 3 weeks after stopping FOS intake. The fecal pH before FOS intake was $6.56{\pm}0.09%$, while it decreased significantly (p<0.01) during the period of FOS intake. The pH reduction lasted for 4 weeks after stopping the intake of FOS. The log fecal number of Bifidobacteria during the period was $7.88{\pm}1.43%$ (CFU/g of wet feces) and it increased significantly during the FOS intake. After stopping the intake of FOS, the number of Bifidobacteria returned to those of control period. The log fecal number of Lactobacilli before the intake was $6.76{\pm}1.34%$ and significantly increased in 3 weeks due to the FOS intake. After stopping the intake of FOS, however, the number of Lactobacilli returned to those of control period. No remarkable changes were observed in the number of coliforms throughout all durations.

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Characterization of Umbilical Cord-derived Stem Cells during Expansion in Vitro (탯줄유래 줄기세포의 계대배양에 따른 특성 변화의 분석)

  • Park, Se-Ah;Kang, Hyun-Mi;Heo, Jin-Yeong;Yoon, Jin-Ah;Kim, Hae-Kwon
    • Clinical and Experimental Reproductive Medicine
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    • v.36 no.1
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    • pp.23-34
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    • 2009
  • Objectives: Mesenchymal stem cells (MSC) comprise a promising tool for cellular therapy. It is known that long-term in vitro culture of human bone marrow and adipose tissue derived-MSCs lead to a reduction of life span and a change of stem-like characters. The aim of our study was to examine whether stem cell properties of human umbilical cord-derived stem cells (HUC) could be affected by in vitro expansion. Methods: HUC were isolated from human umbilical cord and cultured for 10 passages in vitro. Morphology and population doubling time (PDT) were investigated, and changes of stem cell properties were examined using RT-PCR and immunocytochemistry during serial subcultures. Results: Morphology and PDT of HUC began to change slightly from the 7th passage (p7). Expression level of nestin and vimentin mRNAs increased along with the culture period from p4 until p10. In contrast, expression level of SCF mRNA decreased during the same culture period. Expression level of Oct-4 and HNF-4${\alpha}$ mRNAs was not significantly changed throughout the culture period until p10. Expression level of BMP-4, FGF-5, NCAM and HLA-ABC mRNAs appeared to increase as the culture continued, however, the difference was not significant. Immunocytochemical studies showed that HUC at p3, p6 and p9 positively were stained with antibodies against SSEA-3 and SSEA-4 proteins. Interestingly, staining intensity of HUC for ICAM-1 and HLA-ABC gradually increased throughout the culture period. Intensity against thy-1 and fibronectin antibodies increased at p9 while that against TRA-1-60 and VCAM-1 antibodies began to decrease at p6 until p9. Conclusions: These results suggest that HUC change some of their stem cell characteristics during in vitro culture. Development of culture system might be needed for the maintenance of characteristics.

Risk Factors in Relation to Blood Pressure in Schoolchildren (국민학교 학동의 혈압에 관련된 요인분석)

  • Lee, Dong-Soo;Lee, Kyeong-Soo;Kang, Pock-Soo
    • Journal of Yeungnam Medical Science
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    • v.10 no.2
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    • pp.345-359
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    • 1993
  • This study was conducted to identify the factors related to the blood pressure in school children. The study subjects were 144 boys and 140 girls aged 10 years old, 4th grade student of one elementary school in Taegu City. Blood pressure and 18 variables for 284 school children were measured in May 1992. A weight-for-height index, R$\ddot{o}$hrer index(weight/$height^3$) was calculated for each individual as a measure of obesity. Body fat percent, fat weight, and lean body mass were measured by impedance fat meter(Model SIF-891). Serum total cholesterol, high-density lipoprotein, triglyceride, uric acid, total protein, and electrolyte were measured by automated clinical chemistry analyzer(Hitachi 4020). Low-density lipoprotein and atherogenic index were calculated by the equation. The variables assessed were sex, systolic blood pressure, diastolic blood pressure. R$\ddot{o}$hrer index, body fat percent, body fat weight, lean body mass, total cholesterol, high-density lipoprotein, low-density lipoprotein, uric acid, total protein, serum electrolyte and blood glucose. The mean systolic and diastolic blood pressures for boys were 104.1mmHg and 66.6mmHg and those for girls were 102.9mmHg and 67.5mmHg. Systolic blood pressure of boys was positively correlated with height, weight, R$\ddot{o}$hrer index, fat weight, lean body mass, and triglyceride. Systolic blood pressure of girls was positively correlated with height, weight, lean body mass, and uric acid. Diastolic blood pressure of boys was positively correlated with weight. R$\ddot{o}$hrer index, and lean body mass. Diastolic blood pressure of girls was positively correlated with height, weight, fat weight, and lean body mass and negatively with total serum protein. In multiple regression analysis for the systolic blood pressure, the significant independent variables were R$\ddot{o}$hrer index and serum sodium in boys, and serum uric acid, fat percent, and R$\ddot{o}$hrer index in girls. In multiple regression analysis of the diastolic blood pressure, the significant independent variables were R$\ddot{o}$hrer index in boys and total serum protein in girls. This study indicated that important factors influencing blood pressure in school children were R$\ddot{o}$hrer index, fat percent, serum sodium, uric acid, and total protein. R$\ddot{o}$hrer index was most important among these. It is recommended to enforce the nutritional education for diet control of obesity to prevent hypertention in children and to adopt healthy life-styles that promote good health and prevent development of cardiovascular risk factors.

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THE RELIABILITY AND VALIDITY OF KOREAN CONNERS PARENT AND TEACHER RATING SCALE (한국어판 Conners 부모 및 교사용 평가 척도의 신뢰도와 타당도에 대한 예비적 연구)

  • Park, Eun-Hee;So, Yu-Kyoung;Choi, Nak-Kyung;Kim, Se-Joo;Noh, Joo-Sun;Ko, Yun-Joo;Kim, Young-Shin
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.14 no.2
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    • pp.183-196
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    • 2003
  • Objectives:The Purpose of this study is to investigate the reliability and validity of Korean Parent and Teacher Conners' Rating Scale. Methods:Randomly selected 1st to 6th graders of the two elementary schools(N=1044) in Anyang City participated in the study. Children diagnosed with ADHD(N=23) at the child and adolescent clinic at a university affiliated hospital were included in the study for the analysis of clinical validity of the scales. Parent and teacher completed Korean Conners' Rating Scale and Korean-ADHD Rating Scale(KARS). In addition, parents completed Korean-Children Behavior CheckList(K-CBCL). Descriptive statistics, t-test, and analysis of variance were performed. Results:Scores of Korean Conners' Parents Rating Scale were significantly correlated with those of Korean Conners Teacher Rating Scale. High internal consistency reliability were demonstrated in both parent and teacher rating scales. There were significant correlations among sub-scales of Conners' Rating Scales, K-CBCL and K-ARS. Factor analyses revealed that the K-CTRS had three-factor structure (Inattention-Passivity, Hyperactivity, Conduct Problem) and the K-CPRS had five-factor structure(Impulsive-Hyperactive, Conduct Problem I, Anxiety, Psychosomatic, Conduct Problem II). Conners' Rating Scales effectively distinguish children with ADHD from children without ADHD. Conclusion:Korean Parent and Teacher Conners' Rating Scales are valid and reliable instruments that are useful for screening and identifying childhood problem behaviors. Future studies are required with a larger number of sample sizes including adolescents from various geographic regions.

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History of the Korean Society of Applied Entomology for its First Fifty Years (한국응용곤충학회의 첫 50년 역사)

  • Boo, Kyung-Saeng
    • Korean journal of applied entomology
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    • v.51 no.2
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    • pp.171-190
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    • 2012
  • The Korean Society of Applied Entomology (KSAE) celebrates its First 50 years history this year, 2011. It began in the year 1962, as the Korean Society of Plant Protection (KSPP) to discuss all aspects of plant protection including entomology and plant pathology. At that time it was one of the earliest scientific ones among agricultural societies in Korea. Before liberation from the Japanese colonial rule there were a few scientific societies for Japanese scientists only in the Korean Peninsula. It seemed that there was a single exception, in medical field, formed by and operated for Korean ethnics. Right after the liberation, Korean scientists rushed to form new scientific societies in the fields of mechanical engineering, architecture, textile, internal medicine, biology, etc. in 1945, mathematics, chemistry, metallurgy, etc. in 1946, and so on. But agricultural scientists had to wait for more time before setting up their own scientific society, Korean Agricultural Society(韓國農學會), comprising all agricultural subfields, in 1954. They had annual meetings and published their own journal every year until 1962. Then those working in the plant protection field established their own KSPP, right after their section meeting in 1962. At that time the total number of participants for KSPP were only around 50. KSPP scientists were interested in plant pathology, agricultural chemicals, weed science, or bioclimate, besides entomology. They had annual meetings once or twice a year until 1987 and published their own journal, Korean Journal of Plant Protection (KJPP), once a year at the earlier years but soon gradually increasing the frequency to four times a year later. Articles on entomology and plant pathology occupied about 40% each, but the number of oral or posters were a little bit higher on plant pathology than entomology, with the rest on nematology, agricultural chemicals, or soil microarthropods. There also had a number of symposia and special lectures. The presidentship lasted for two years and most of president served only one term, except for the first two. The current president should be $28^{th}$. In the year 1988, KSPP had to be transformed into the applied entomology society, Korean Society of Applied Entomology (KSAE), because most of plant pathologists participating left the society to set up their own one, Korean Society of Plant Pathology in 1984. Since that time the Society concentrates on entomology, basic and applied, with some notes on nematology, acarology, soil microarthropods, agricultural chemicals, etc. The Society has been hosting annual meetings at least twice a year with special lectures and symposia, from time to time, on various topics. It also hosted international symposia including binational scientific meetings twice with two different Japanese (applied entomology in 2003 and acarology in 2009) societies and the Asia-Pacific Congress of Entomology in 2005. The regular society meeting of this year, 2011, turns out to be the 43rd and this autumn non-regular meeting would be the 42nd. It has been publishing two different scientific journals, Korean Journal of Applied Entomology (KJAE) since 1988 and the Journal of Asia-Pacific Entomology (JAPE) since 1998. Both journals are published 4 times a year, with articles written in Korean or English in the first, but those in English only in the latter with cooperation from the Taiwan Entomological Society and the Malaysian Plant Protection Society since 2008. It is now enlisted as one of those SCI(science citation index) extended. The highest number of topics discussed at their annual meetings was on ecology, behavior, and host resistance. But at the annual meetings jointly with the Korean Society of Entomology, members were more interested in basic aspects, instead of applied aspects, such as physiology and molecular biology fields. Among those societies related to entomology and plant protection, plant pathology, pesticide, and applied entomology societies are almost similar in membership, but entomology and plant pathology societies are publishing more number of articles than any others. The Society is running beautifully, but there are a few points to be made for further improvement. First, the articles or posters should be correctly categorized on the journals or proceedings. It may be a good idea to ask members to give their own version of correct category for their submissions, either oral or poster or written publication. The category should be classified detailed as much as possible (one kind of example would be systematics, morphology, evolution, ecology, behavior, host preference or resistance, physiology, anatomy, chemical ecology, molecular biology, pathology, chemical control, insecticides, insecticide resistance, biocontrol, biorational control, natural enemies, agricultural pest, forest pest, medical pest, etc.) and such scheme should be given to members beforehand. The members should give one or two, first and second, choices when submitting, if they want. Then the categories might be combined or grouped during editing for optimal arrangement for journals or proceedings. Secondly the journals should carry complete content of the particular year and author index at the last issue of that year. I would also like to have other information, such as awards and awardees in handy way. I could not find any document for listing awards. Such information or article categorization may be assigned to one of the vice presidents. I would rather strongly recommend that the society should give more time and energy on archive management to keep better and more correct history records.

Annual Occurrent Pattern of Scirtothrips dorsalis (Thysanoptera: Thripidae) on Citrus Trees and Surrounding Host Plants (감귤원과 그 주변 기주식물에서 볼록총채벌레의 연중발생 양상)

  • Song, Jeong Heub;Kim, Chang Seog;Yang, Young Taek;Hong, Soon Yeong;Lee, Shin Chan
    • Korean journal of applied entomology
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    • v.52 no.3
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    • pp.185-191
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    • 2013
  • The damage of citrus by Scirtothrips dorsalis Hood appears to have increased since 2007 in Jeju, although the characteristics of seasonal abundance are not clear. This study was conducted to investigate the relationship between host plants and the seasonal abundance of S. dorsalis, observing plants distributed around citrus orchards. The host plants of S. dorsalis surrounding citrus orchards were determined to include 32 families, 54 species: 39 woody plant species and 15 herbaceous plant species. The host plants which related to the occurrence of 1st generation of S. dorsalis were Lonicera japonica, Clematis apiifolia, Hedera rhombea, and Viburnum awabuki. The occurrence of 1st generation S. dorsalis was estimated to be due to overwintered female adults having laid eggs into those plants from late March to early April, and the new adults having emerged from late April to late May. The host plants which were associated with fruit damage of citrus were Mallotus japonicus, and Camellia japonica, as well as creeping plants such as Clematis apiifolia, Paederia scandens and Cayratia japonica. The adult phase density of S. dorsalis caught on yellow-color sticky traps placed on the citrus trees on the edge of the citrus orchard. S. dorsalis were predominantly 3rd generation from late of June to early of July, and 6th generation from late of August to early of September, and their numbers were directly related to the degree of damage caused to the citrus fruit. The density of S. dorsalis depended on the number of new growing shoots of host plants, which indicated that the immigration of adults of S. dorsalis to the citrus was based in the suitability of host plants surrounding the orchards.

The Development and Application of Informal Gifted and Talented Education program utilizing Local Resources in Yeongheung Island (영흥도 지역자원을 활용한 학교 밖 영재 프로그램 개발 및 적용 효과)

  • Ock, Seong-Hyun;Choi, SunYoung
    • Journal of Science Education
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    • v.38 no.2
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    • pp.356-375
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    • 2014
  • The purpose of this study was to develop the informal education based elementary gifted education program utilizing local resources in Yeongheung island for enhancing the creative problem solving, science process skills, scientific attitudes. This program was totally consisted 36 lessons and 3 part. For this purpose, 7 weeks' informal gifted education program utilizing local resources was treated to a class students who were 5th graders of elementary school in Yeongheung island. The results of this study were as follows : First, the change of science creative problem solving ability in the gifted class was found statistically meaningful difference. Second, science process skills were showed statistically meaningful difference. Third, scientific attitudes were not showed statistically meaningful difference. Fourth, the student responses about effectiveness of this programs were generally positive. Therefore, the gifted education programs showed meaningful results. Based on the results of this study, a number of studies to overcome the limitations will be needed.

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The Cosmetic Operation without Healing Purpose - A comparative insight into the ruling of BSG and BGH - (미용성형의료 - 우리 판결례와 독일 판결례의 비교·분석적 소고 -)

  • Ahn, Bup-Young
    • The Korean Society of Law and Medicine
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    • v.16 no.1
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    • pp.3-82
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    • 2015
  • This paper is concerned in the theme of the liability for the breach of duty to inform(Haftungszurechnung der $Aufkl{\ddot{a}}rungspflichtverletzung$) and the malpractice of cosmetic surgery. Here, the terms, treatments for healing purposes and purely medical-technical cosmetic operations are well integrated in the category of "medical conduct(medizinische Handlung)" within the meaning of the public and administrative 'Medical Law'. In the judgment of 6. 13. 2013 Az. 2012DA94865 provides the KHGH(Korean Highest Court of Justice) to inform the patient about the prospects and risks of cosmetic surgery(Infrabrow Excision Blepharoplasty) stringent requirements, similar to the judicature of BGH(cf. BGH, Urt. v. 6. 11. 1990, Az.: VI ZR 8/90). Even in the judgment of 5. 12. 2014 Az. 2013GASO865646 the SZLG(Seoul Central Regional Court) recognizes the physician contract for 'cosmetic septoplasty' as a sort of contract for work. The medical treatment(${\ddot{a}}rztliche$ Heilbehandlung) is still regarded as a prototype of the medical activity, therefore in the meaning of the 'Civil Law(KBGB)', its term needs to be used immediately for healing purposes. The cosmetic operation, desired by a patient, differs from the healing treatment by the element of "indication" and the fact that the "healing purpose(Heilzweck)" itself is missing. In comparative context - methodically fully aware that the unreflective term transfer between different laws might contradict their legal purposes - a series of judgments BSG(BSGE 63, 83, BSGE 72, 96, BSGE, 82, 158, BSGE 93, 252 etc.) and some judgments of LSG are reviewed. In addition, also the dogmatic topic for the "legal natur of a medical treatment contract" is to reconsider by comparative introducing BGHZ 63, 306. Now in view of the current state of greater popularity of artificial cosmetic surgery still indeed is the sentences: The doctor is minister naturae, a helper of nature. A doctor promises regularly only the proper treatment of the patient, but the contractual liability for work should not be excluded in medical conditions for cosmetic surgeries altogether. "With cosmetic operations, seeking to eliminate the external deformities, the doctor may miss the medical profession entirely." - A. Laufs, Medical Law, 5th ed. P. 18.

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A Study of Dual-probe Esophageal pH Meter in Children with Gastroesophageal Reflux (위식도 역류질환 환아에서 보행성 이중채널 식도내 산도 검사에 대한 연구)

  • Ahn, Young-Joon;Moon, Kyung-Rye
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.3 no.1
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    • pp.9-16
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    • 2000
  • Purpose: The aim of this study was to determine how much acid exposure would occur in the proximal esophagus, both in normal and in patients with abnormal distal esophageal acid exposure. Methods: Fourty-six patients with suspected GER were classified into two groups, 24 patients with pathological distal reflux (group I); 22 patients with normal distal reflux (group II). The ambulatory dual-probe esophageal pH monitoring was performed for 18-24hr. The abnormal reflux was defined when the percent of time that pH was below 4.0 exceeded the 95th percentile of normal value. Results: The siginficant differences between distal and proximal esophageal pH recordings in group I persisted for all parameters except for the longest episode, but didn't persist in group II. At the distal esophageal site, the median percent time with pH<4.0 in group I was 19.3 and significantly higher than at proximal site. Half of patients with pathological distal reflux also had proximal acid reflux. Correlation coefficients between the distal and proximal esophageal sites in group I of the number of reflux episodes and time of the longest episode were 0.451 and 0.646 respectively. Conclusion: The 50 percent of patients with pathological distal acid reflux also had abnormal acid exposure in the proximal esophageal site. Therefore, we recommand simultaneous pH recordings from dual probe esophageal sites in children with gastroesophageal reflux.

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Factors Related to the Subjective Well-being and Depression Symptoms among Elderly in Rural Areas (일부 농촌지역 노인들의 주관적 행복감과 우울에 관련된 요인)

  • Cho, Kwang-Hee;Jeong, Yong-Jun;Cho, Young-Chae
    • Journal of agricultural medicine and community health
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    • v.28 no.2
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    • pp.71-85
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    • 2003
  • Objectives: The present study was directed at revealing the influence of various life styles on the subjective well-being and depressive states, and their related factors among the elderly. Methods: The interviews were given to 454 elderly people aged over 65 (197 male and 257 female) in rural areas of Chungnam Province during the 3-month period from July 1st to Sep. 30th, 2002. The interview contents for the elderly included social demographic characteristics, activities of daily living(ADL) whether independent of others or not, subjective well-being, Zung's self-rating depression scale(SDS), etc. Results: The male elderly showed significantly higher scores in subjective well-being than the female, and with regard to Zung's depression scores, they were significantly higher in the female than the male elderly. Based on the correlation between scores of subjective well-being and its related factors, there was a significant, positive correlation in both sexes with whether or not participation in the social gatherings and the degree of satisfaction with subjective health status. The factors shown to be negatively correlated with depression scores in both sexes were whether or not participation in the social gatherings, the degree of satisfaction with the subjective health status, economic conditions, and ADL scores. The factors influencing on the subjective well-being included depressive states, eating habits, dwelling states, ADL scores, and physical activity. Those influencing on the depressive states were the degree of satisfaction with the subjective health status, physical activity, sexes, smoking, ADL scores, economic conditions, eating habits and whether or not participation in the social gatherings. Conclusions: Consequently, the subjective well-being as well as physical activity and economic conditions were shown to be the important factors for the healthy elderly life.

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