• Title/Summary/Keyword: Test-day

Search Result 4,497, Processing Time 0.03 seconds

Analytical Validation of Rosmarinic Acid in Water Extract of Perilla frutescens Britton var. acuta Kudo as Functional Health Ingredient (건강기능식품 기능성 원료로써 장흥 차조기 열수 추출물의 지표성분인 로즈마린산 분석법 검증)

  • Park, Sung-Yong;Kim, Jung-Eun;Choi, Chul-Yung;Lee, Dong-Wook;Kim, Ki-Man;Yoon, Goo;Yoon, In-Su;Moon, Hong-Seop;Cho, Seung-Sik
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.44 no.1
    • /
    • pp.85-88
    • /
    • 2015
  • This study attempted to establish an HPLC analysis method for determination of marker compounds as a part of material standardization for the development of health functional food materials from Perilla frutescens Britton var. acuta Kudo. The quantitative determination method of rosmarinic acid as a marker compound of P. frutescens Britton var. acuta Kudo extract (PFE) was optimized by HPLC analysis using a C18 column ($4.6{\times}150mm$, $5{\mu}m$) with 0.1% acetic acid as the elution gradient and methanol as the mobile phase at a flow rate of 1 mL/min and detection wavelength of 280 nm. The HPLC/UV method was applied successfully to quantification of the marker compound in PFE after validation of the method with linearity, accuracy, and precision. The method showed high linearity in the calibration curve at a coefficient of correlation ($R^2$) of 0.9995, and the limit of detection and limit of quantitation were $0.36{\mu}g/mL$ and $1.2{\mu}g/mL$, respectively. Relative standard deviation (RSD) values of data from intra- and inter-day precision were less than 3.21% and 1.43%, respectively. Recovery rate test at rosmarinic acid concentrations of 12.5, 25 and $50{\mu}g/mL$ scored between 97.04~98.98% with RSD values from 0.25~1.97%. These results indicate that the established HPLC method is very useful for the determination of marker compound in PFE to develop a health functional material.

The Oral Health Behaviors for Children and Teachers by Teacher-Supervised Toothbrushing in Community Child Center (지역아동센터 교사의 칫솔질 지도여부에 따른 아동과 교사의 구강보건행태)

  • Shin, Sun-Jung;Jang, Jung-Yoo;Kim, Chang-Hee
    • Journal of dental hygiene science
    • /
    • v.12 no.6
    • /
    • pp.572-582
    • /
    • 2012
  • The purpose of this research was to compare the oral health behaviors for children and teachers in toothbrushing facilities installed community child center related with teacher-supervised toothbrushing. This research was follow-up study from the Korean Dental Hygienists Association third project '2010 program of making of the diamond tooth'. 266 child support teachers were invited and 201 were included in the study (response rate: 75.6%). Statistical analysis conducted using PASW ver. 18.0 for Windows. The difference on the distribution of independent variables related with teacher-supervised toothbrushing was verified with chi-square test. The results of this research could be summarized as follows: The children in the teacher-supervised toothbrushing community child center, the practice rate of toothbrushing was higher than the children in the non-supervised toothbrushing community child center (p<0.05). Teachers offered children one of snacks and drinks per day independently of teacher-supervised toothbrushing (p>0.05). Most of participated teachers accepted to be responsible for oral health of children (73.4%) and demanded that improve facilities for a toothbrushing in community child center (77.2%). In conclusion, this study suggests that a programme of daily teacher-supervised toothbrushing can be effectively targeted into community child center.

Antibacterial Activity of Ceftizoxime Against Gram Negative Enteric Bacteria in vitro and in vivo (Ceftizoxime의 장내세균에 대한 시험관내 및 생체내 항균효과)

  • Byun, Woo-Mok;Chang, Jae-Chun;Park, Bok-Hwan;Kim, Hee-Sun;Kim, Sung-Kwang
    • Journal of Yeungnam Medical Science
    • /
    • v.6 no.1
    • /
    • pp.59-68
    • /
    • 1989
  • Ceftizoxime sodium is a new synthetic ${\beta}$-lactam antibiotic combining potent antibacterial activity with high stability to a wide range of bacterial ${\beta}$-lactamase. This experiment was achieved to evaluate the antibacterial activities of ceftizoxime sodium againist Gram negative enteric bacteria isolated from in outpatient visiting Yeungnam university hospital and to study the emergence of drug induced bacterial varients which resist to ceftizoxime in vitro. The antibacterial activity of the ceftizoxime was compared with that of antibiotics and its effect on population of normal intestinal flora in mice was observed. The results are summarized as follows : 1. Highly effective antibacterial activity of ceftizoxime against Gram negative enteric bacilli was demonstrated and this antibacterial activity was superior to that of ampicillin. 2. Several test strains shows multiple antibiotic resistence. Among 15 strains of Escherichia coli, 1 strain was resistent to ampicillin, cefadroxyl, gentamicin, tetracycline, and 2 strains were resistent to ampicillin, cefadroxyl, tetracycline, five strains of Escherichia coli and Enterobacter cloacae was resistent to amplicillin, tetracycline and Shigella dysenteria was resistent to ampicillin, gentamicin, tetracycline. 3. The frequency of in vitro emergence of resistent varients among ceftizoxime sensitive bacteria in the presence of increasing concentrations of the compound was found to be low. 4. Plasmid was isolated in 6 of 9 strains (6 strains of Escherichia coli, Shigella dysenteriae, Enterobacter cloaceae and Salmonella typhi) That showed different antibiotic resistance. They were 5 strains of Escherichia coli and 1 strain of Shigella dysenteriae. However, plasmid could not be considered as a hallmark for antibiotic resistance by this. Further studies with curing experiment are to be accomplished for this purpose. 5. Changes in the bacterial count of normal intestinal flora following 25mg/kg/day administration of ceftizoxime over S consecutive days were not significant. In conclusion, ceftizoxime appeared to be a drug of choice in the treatment of Gram negative enteric bacilli infection.

  • PDF

Naked Waxy Barley Cultivar "Jinjuchal" with High Whiteness after Cooking and High β-glucan Content (취반 후 백도가 좋고 베타글루칸 함량이 높은 찰성 쌀보리 "진주찰")

  • Lee, Mi-Ja;Seo, Jae-Hwan;Kim, Yang-Kil;Park, Jong-Chul;Choi, Jae-Seong;Park, Tae-Il;Hyun, Jong-Nae;Kim, Jung-Gon
    • Korean Journal of Breeding Science
    • /
    • v.41 no.3
    • /
    • pp.299-305
    • /
    • 2009
  • A new six-rowed naked barley cultivar "Jinjuchal" with high whiteness after cooking and high $\beta$-glucan content was developed from the cross between 'Jinmichapssalbori' with high winter hardiness, lodging tolerance, grain whiteness and pearling yield, and 'Suwon 333' with waxy endosperm by the Honam Agricultural Research Institute (HARI), NICS, RDA in 2007. An elite line, SB962002G-B-B-B-84-4 was selected in 2002 and designated as 'Iksan 79' It showed good agronomic performance in the regional yield trials (RYT) from 2005 to 2007 and was released with the name of "Jinjuchal" possessing high whiteness and low proanthocyanidin waxy endosperm. The average heading and maturing dates of "Jinjuchal" were April 27 and June 1 in paddy field, which were same and one day later than those of the check cultivar 'Saechalssalbori' respectively. The new cultivar, "Jinjuchal" had 81 cm of culm length that was 1cm longer than that of 'Saechalssalbori' and 4.9 cm of spike length. It showed 673 spikes per $m^2$, 56 grains per spike, 27.0 g of 1,000-grain weight, and 752 g of test weight. "Jinjuchal" showed stronger hardiness and better resistance to powdery mildew and BaYMV (Barley yellow mosaic virus) than those of the check cultivar, 'Saechalssalbori' It showed higher $\beta$-glucan content(8.4%) and water absorption rate than those of the check cultivar, 'Saechalssalbori' Its average yield of the pearled grain in the regional yield trial was 3.79 MT/ha in upland, and 3.73 MT/ha in paddy field, which were 1% lower and 3% higher than those of the check cultivar, respectively. Total phenol and proanthocyanidin contents were 4.2 and 0.2 mg/g, respectively. This cultivar is suitable for the area of the daily minimum temperature above $-6^{\circ}C$ in January in Korean peninsula.

Application of Chlorophyll Fluorescence Parameters for the Detection of Water Stress Ranges in Grafted Watermelon Seedlings (수박접목묘의 건조스트레스 범위 탐지를 위한 엽록소형광 지수의 적용)

  • Shin, Yu Kyeong;Kim, Yong Hyeon;Lee, Jun Gu
    • Journal of Bio-Environment Control
    • /
    • v.28 no.4
    • /
    • pp.461-470
    • /
    • 2019
  • This study was carried out to quantify the drought stress in grafted watermelon seedlings non-destructively by using chlorophyll fluorescence (CF) imaging technique rather than the visual judgment. Six-day old watermelon seedlings were grown under uniform irrigation for 3 days, and then given drought stress. Afterward, the sensor for the measurement of water content in plug tray cell unit was used to classify the drought-stress level into nine groups from D1 (53.0%, sufficient moisture state) to D9 (15.7%, extremely dry stress), and the 16 CF parameters were measured. In addition, re-irrigation was performed on the drought stressed seedlings(D5 - D9) to determine the growth and photosynthesis recovery level, which was not confirmed by visual judgment. The kinetic curve patterns of CF in three different drought stressed seedling groups were found to be different for the early detection of drought stress. All the 16 CF parameters decreased continuously with exposure to drought stress and drastically decreased from D5 (32.1%) where the visual judgment was possible. The fluorescence decline ratio (Rfd_Lss) started to decrease from the initial drought stress level (D5 - D6), and the Maximum PSII quantum yield (Fv/Fm) was significantly decreased in the later extreme drought stress range (D7 - D9) by re-irrigation recovery test. Thus, Rfd_Lss and Fv/Fm parameters were finally selected as potent indicators of growth and photosynthesis recovery in the initial and later stages of drought stress. Also, to the differences in the numerical values of the individual chlorophyll fluorescence parameters, the drought stress level was intuitively confirmed through the image. These results indicate that Rfd and Fv/Fm can be considered as potential CF parameters for the detection of low and extremely high drought stress, respectively. Furthermore, Fv/Fm can be considered as the best CF parameters for recovery at re-irrigation.

Neotectonic Crustal Deformation and Current Stress Field in the Korean Peninsula and Their Tectonic Implications: A Review (한반도 신기 지각변형과 현생 응력장 그리고 지구조적 의미: 논평)

  • Kim, Min-Cheol;Jung, Soohwan;Yoon, Sangwon;Jeong, Rae-Yoon;Song, Cheol Woo;Son, Moon
    • The Journal of the Petrological Society of Korea
    • /
    • v.25 no.3
    • /
    • pp.169-193
    • /
    • 2016
  • In order to characterize the Neotectonic crustal deformation and current stress field in and around the Korean Peninsula and to interpret their tectonic implications, this paper synthetically analyzes the previous Quaternary fault and focal mechanism solution data and recent geotechnical in-situ stress data and examines the characteristics of crustal deformations and tectonic settings in and around East Asia after the Miocene. Most of the Quaternary fault outcrops in SE Korea occur along major inherited fault zones and show a NS-striking top-to-the-west thrust geometry, indicating that the faults were produced by local reactivation of appropriately oriented preexisting weaknesses under EW-trending pure compressional stress field. The focal mechanism solutions in and around the Korean Peninsula disclose that strike-slip faulting containing some reverse-slip component and reverse-slip faulting are significantly dominant on land and in sea area, respectively. The P-axes are horizontally clustered in ENE-WSW direction, whereas the T-axes are girdle-distributed in NNW direction. The geotechnical in-situ stress data in South Korea also indicate the ENE-trending maximum horizontal stress. The current crustal deformation in the Korean Peninsula is thus characterized by crustal contraction under regional ENE-WSW or E-W compression stress field. Based on the regional stress trajectories in and around East Asia, the current stress regime is interpreted to have resulted from the cooperation of westward shallow subduction of the Pacific Plate and collision of Indian and Eurasian continents, whereas the Philippine Sea plate have not a decisive effect on the stress-regime in the Korean Peninsula due to its high-angle subduction that resulted in dominant crust extension of the back-arc region. It is also interpreted that the Neotectonic crustal deformation and present-day tectonic setting of East Asia commenced with the change of the Pacific Plate motion during 5~3.2 Ma.

Comparison of Reflectance and Vegetation Index Changes by Type of UAV-Mounted Multi-Spectral Sensors (무인비행체 탑재 다중분광 센서별 반사율 및 식생지수 변화 비교)

  • Lee, Kyung-do;Ahn, Ho-yong;Ryu, Jae-hyun;So, Kyu-ho;Na, Sang-il
    • Korean Journal of Remote Sensing
    • /
    • v.37 no.5_1
    • /
    • pp.947-958
    • /
    • 2021
  • This study was conducted to provide basic data for crop monitoring by comparing and analyzing changes in reflectance and vegetation index by sensor of multi-spectral sensors mounted on unmanned aerial vehicles. For four types of unmanned aerial vehicle-mounted multispectral sensors, such as RedEdge-MX, S110 NIR, Sequioa, and P4M, on September 14 and September 15, 2020, aerial images were taken, once in the morning and in the afternoon, a total of 4 times, and reflectance and vegetation index were calculated and compared. In the case of reflectance, the time-series coefficient of variation of all sensors showed an average value of about 10% or more, indicating that there is a limit to its use. The coefficient of variation of the vegetation index by sensor for the crop test group showed an average value of 1.2 to 3.6% in the crop experimental sites with high vitality due to thick vegetation, showing variability within 5%. However, this was a higher value than the coefficient of variation on a clear day, and it is estimated that the weather conditions such as clouds were different in the morning and afternoon during the experiment period. It is thought that it is necessary to establish and implement a UAV flight plan. As a result of comparing the NDVI between the multi-spectral sensors of the unmanned aerial vehicle, in this experiment, it is thought that the RedEdeg-MX sensor can be used together without special correction of the NDVI value even if several sensors of the same type are used in a stable light environment. RedEdge-MX, P4M, and Sequioa sensors showed a linear relationship with each other, but supplementary experiments are needed to evaluate joint utilization through off-set correction between vegetation indices.

A Study on the Current State of the Integrated Human Rights of the Elderly in Rural Areas of South Korea (농촌지역 거주 노인의 통합적 인권보장 실태에 관한 연구)

  • Ahn, Joonhee;Kim, MeeHye;Chung, SoonDool;Kim, SooJin
    • 한국노년학
    • /
    • v.38 no.3
    • /
    • pp.569-592
    • /
    • 2018
  • This study purported to investigate the current state of human rights of older adults residing in rural areas of Korea. The study utilized, as an analytic framework, 4 priority directions (1. "older persons and development", 2. "rural area development", 3. "advancing health and well-being into old age", and 4. "ensuring enabling and supportive environments") with 13 task actions recommended by Madrid International Plan of Action on Ageing (MIPAA). Furthermore, the study examined gender differences in all items included in the analytic framework. Data was collected by the face-to-face survey on 800 subjects aged 65 and over. Statistical analyses were conducted using STATA 13.0 program. The main results were summarized in order of 4 priority directions as follows. First, average working hours per day were 6.2, and men reportedly participated in economic activities and needed job training more than women, while women participated in lifelong education programs more than men. Awareness of fire and disaster prevention facilities was low in both genders. Second, accessibility to the support center for the elderly living alone as well as protective services for the vulnerable elderly was found to be low. IT-based services and networking were used more by men than women, and specifically, IT-based financial transactions and welfare services were least used. Third, medical check-ups and vaccinations were well received, while consistent treatments for chronic illnesses and long-term care services were relatively less given. In addition, accessibility to mental health service centers was considerably low. Fourth, although old house structures and the lack of convenience facilities were found to be circumstantial risk factors for these elders, experiences of receiving housing support services were scarce. The elderly were found to rely more on informal care, and concerns for their care were higher in women than men. Plus, accessibility to elderly abuse services was markedly low. Based on these results, discussed were implications for implementing policies and practical interventions to raise the levels of the human rights for this population.

Characteristics of Indoor Particulate Matter Concentrations by Size at an Apartment House During Dusty-Day (황사 발생시 아파트 실내에서 미세먼지 크기별 농도 특성)

  • Joo, Sang-Woo;Ji, Jun-Ho
    • Particle and aerosol research
    • /
    • v.15 no.1
    • /
    • pp.37-44
    • /
    • 2019
  • It is recommended for the public to stay at home and to close the doors and windows when a high-particulate-matter environment such as a yellow sand event occurs outside. However, there are lack of empirical studies describing how much outdoor PM infiltrates into a closed house and how much indoor PM an inhabitant is exposed to during the period. In this study, the $PM_{10}$ and $PM_{2.5}$ were measured at the kitchen in an apartment house by an optical particle counter for 3 days including a yellow sand event. The outdoor PMs and the outdoor wind speeds were referred from surrounding weather stations. We analyzed the penetration of $PM_{10-2.5}$ and $PM_{2.5}$ at the test house against the outdoor wind speed supposed corresponding to the change of air exchange rate. In addition, the effect of an indoor activity on change in the indoor PM was investigated. In result, the indoor $PM_{10-2.5}$ was very low even a yellow sand event occurred outside; rather, a contribution of indoor activities to increase in $PM_{10-2.5}$ was higher. In contrast, the indoor $PM_{2.5}$ fluctuated following the outdoor $PM_{2.5}$ trend at high wind speeds or remained almost constant at low wind speed.

Evaluation of Shelf Life of Non-Pasteurized Egg Yolks, Egg Whites, and Whole Egg Liquid Products in Korea (국내 비살균 전란액, 난백액, 난황액의 유통기한 평가)

  • Kim, Young-Jo;Moon, Hye-Jin;Song, Bo-Ra;Lim, Jong-Soo;Heo, Eun-Jeong;Park, Hyun-Jung;Wee, Sung-Hwan;Moon, Jin-San
    • Journal of Food Hygiene and Safety
    • /
    • v.34 no.1
    • /
    • pp.94-99
    • /
    • 2019
  • The objective of this study is to establish the shelf life of non-pasteurized whole egg, egg yolk and egg white liquid. Each sample was stored for two weeks at $5^{\circ}C$, $10^{\circ}C$, $15^{\circ}C$, and $25^{\circ}C$, and then sensory, microbial, and physicochemical tests were performed periodically. The estimation of shelf life was based on the microbial standards of total viable counts and coliforms. The chemical properties highly correlated with the sensory evaluation were also used. Our results showed that the shelf life was the most influenced by microbial properties. Exceptionally, however, whole egg and white liquid stored at $5^{\circ}C$ and $10^{\circ}C$ with limited bacterial growth were affected by chemical property. The shelf life of the three non-pasteurized liquids was calculated to be less than one day at over $15^{\circ}C$. At $5^{\circ}C$ and $10^{\circ}C$, the shelf life was calculated to be 5 d and 1 d for egg yolk liquid, 5 d and 5 d for egg white, and 7 d and 5 d for whole egg, respectively. Therefore, it is advisable to establish reasonable shelf life in the more specific manner based on consideration of these findings.