• Korean Journal of Microbiology
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• v.50 no.1
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• pp.67-72
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• 2014

Minimal broth containing nicotine as a sole carbon source (MB/N) was used to isolate novel nicotine-degrading bacterial strains from tobacco plants and field soils. Comparative analysis of 16S rRNA gene sequence, phenotypic test and morphological tests showed that the position of these isolates were in the genus Arthrobacter of the family Micrococcaceae. The highest 16S rRNA gene sequence similarity of the isolate NU11 and NU15 to type strains in the genus Arthrobacter were Arthrobacter equi (98.2%) which was presumably a novel strain and Arthrobacter nicotinovorans (99.8%), respectively. Both strain NU11 and NU15 showed rod shaped, Gram-positive characteristics and catalase activity, but did not show oxidase activity. The novel strain NU11 was found to degrade efficiently nicotine in MB/N medium by the analysis of UV absorption spectra and could be used as an organism in bioremediation technique.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.8 no.2
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• pp.167-175
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• 1988

The principle objective of this study is to evaluate the fatigue behavior of structural steel components of highway bridges subjected to service stresses. The main aspects of this investigation are; 1) a measurement and statistical analysis of service stress cycles observed in highway bridge. 2) fatigue tests under equivalent constant-amplitude(CA) loading and simulated variable-amplitude(VA) loading 3) a evaluation of the fatigue behavior under VA-loading by eqivalent root mean cube (RMC) stress range. Theoretically, the RMC model is adequate in evaluation of fatigue behavior under VA-loading, because the regression coefficient (m) of crack growth rate is 3 approximately. The result of fatigue test shows that the RMC model is fitter than the current RMS model in fatigue evaluation under VA-loading. The interaction effects and sequence effects under VA-loading affect little fatigue life of structural components. As the transition rate of stress ranges is higher, the crack growth rate is higher.

• School Mathematics
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• v.11 no.3
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• pp.497-511
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• 2009

This study compared two problem solving instructional approaches, schema based sequence instruction and schema based parallel instruction on word problem solving performance of elementary school students who were in general students group. The subjects totaled 48 third grade students who were exposed to a test that consisted of 9 word problem items of three types for 4 sessions. First of all, the baseline of word problem performance level was measured without any training. During session 1, 2 and 3 participants were put into strategic training groups. The experiment was designed by two between factor(two intervention group and two within factors(two problem types, three sessions). The results of experiment were as follows. Schema based sequence instruction group performed significantly better than students in another group on word problem solving performance. The effect of strategic schema based Instruction revealed that solving word problems relied upon problem types, sessions and input orders which were of great value.

• International Journal of Industrial Entomology and Biomaterials
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• v.5 no.1
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• pp.85-91
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• 2002

The sequences of the internal transcribed spacers (ITS1 and ITS2) and 5.8S ribosomal DNA gene from five Cordyceps species and one Paecilomyces japonica were determined. The total length of the ITSI, 5.8S and ITS2 regions ranged from 528 to 549 bp. When the C. militaris collected from Korea was used as a standard genotype, the sequence showed 88.4%, 88.6%, 91.1% and 86.8% identity to C. pruinosa, C. sphecocephala, C. scarabaeucika and R japonica, respectively, while the lowest identity was found with C. sinensis (75.4%). Interestingly, C. sinensis was phylogenetically distant from the other Cordyceps species. To test geographic variation, furthermore, sequences of the ITS regions in the 8 samples of C. militaris collected from two localities in Korea and China analyzed and compared with the GenBank-searched sequences from Japan and China. The total length of the ITS regions of C. militaris from Korea, Japan and China was completely identical to each other with 528 bp, and the sequence divergence among three localities in pairwise comparisons ranged from 0.2% (1 bp) to 0.4% (2 bp).

• Korean Journal of Veterinary Service
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• v.32 no.2
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• pp.147-153
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• 2009

Salmonella species are the most important etiologic agents of food-borne acute gastroenteritis. The most common serotypes isolated from humans are Salmonella enterica serotype Typhimurium (S. Typhimurium) and S. Enteritidis. Traditional detection methods for Salmonella are based on cultures using selective media and characterization of suspicious colonies by biochemical and serological tests. These methods are generally time-consuming and not so highly sensitive. Recently, the polymerase chain reaction (PCR) has been used as a highly sensitive, specific, and rapid test for the presence of pathogenic bacteria. In this study, a multiplex PCR (m-PCR) was used to detect S. Typhimurium and S. Enteritidis. We selected m-PCR target genes, which were the spv (virulence plasmid specific for S. Enteritidis) and sefA (S. Enteritidis fimbrial antigen) genes, fliC (H1-i antigen specific for S. Typhimurium) and a randomly cloned sequence specific for the genus Salmonella. With m-PCR, random sequence was detected from all strains of Salmonella spp, spv and sefA were detected from all strains of S. Enteritidis (100%), and fliC was detected from all strains of S. Typhimurium (100%). This assay indicate that the specificity of the m-PCR make them potentially valuable tools for detection of S. Typhimurium and S. Enteritidis.

• Journal of Sensor Science and Technology
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• v.8 no.6
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• pp.440-447
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• 1999

Tensile stress loaded on smart composite structures and thermal stress occurred during the during process of the smart composite materials with embedded optical fiber sensors affect directly the mechanical behavior of the embedded optical fiber sensors within the smart composite structures. Stress distribution within the optical fiber sensors varies with respect to the stacking sequence of the composite laminate and the coating conditions of the optical fibers. The cracks occurred within the composite laminate affect not only the fracture of the composite laminate but also the fracture of the optical fiber sensors embedded within the composite laminate. In this study, firstly, stress distribution of the optical fiber sensors embedded within the composite laminate which is subjected to the tensile and thermal stresses was analyzed using Finite Element Method. And, secondly, the effect of the stacking sequence of the composite laminate and the coating conditions of the optical fiber sensors on the stress distribution of the optical fiber sensors was investigated. Finally, the effect of the crack occurred within the smart composite laminate on the fracture behavior of the optical fiber sensors was also observed through the tensile test.

• Transactions of the Korean Society of Automotive Engineers
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• v.14 no.1
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• pp.79-85
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• 2006

CFRP(Carbon Fiber Reinforced Plastics) of the advanced composite materials as structural materials for vehicle, has a wide application in light-weigh structural materials of airplanes, ships and automobiles because of high strength and stiffness, However, there is a design variable to be considered in practical application of the laminate composite materials, these materials are vulnerable to transverse impact. This paper is to study the effects of stacking sequence and curvature on the penetration characteristics of composite laminate shell. They are stacked to $[0_3/90_3]S,\;[90_3/0_3]s\;and\;[0_2/90_3/0]s,\;[90_2/0_3/90]s$ and their interlaminar number two and four. They are manufactured to various curvature radius (R=100, 150, 200mm and $\infty$), When the specimen is subjected to transverse impact by a steel ball, the velocity of the steel ball was measured both before and after impact by determing the time for it to pass two ballistics-screen sensors located a known distance apart. The critical penetration energy of specimen A and B with less interfaces were a little higher than those of C and D. As the curvature increases, the critical penetration energy increases linearly because the resistance to the in-plane deformation as well as bending deformation increases, which need higher critical penetration energy. The specimen A and C have higher critical penetration energy than B and D because of different stacking sequences. We examined crack length through a penetration test. For the specimen A with 2interfaces, the longest circumferential direction crack length were observed on the first interface from the impact point. For the specimen B 4-interface, the longest circumferential direction crack length were observed on the second interface from the impact point.

• The KIPS Transactions:PartB
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• v.14B no.3 s.113
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• pp.163-170
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• 2007

In autonomous navigation of a mobile vehicle or a mobile robot, localization calculated from recognizing its environment is most important factor. Generally, we can determine position and pose of a camera equipped mobile vehicle or mobile robot using INS and GPS but, in this case, we must use enough known ground landmark for accurate localization. hi contrast with homography method to calculate position and pose of a camera by only using the relation of two dimensional feature point between two frames, in this paper, we propose a method to calculate the position and the pose of a camera using relation between the location to predict through perspective transform of 3D feature points obtained by overlaying 3D model with previous frame using GPS and INS input and the location of corresponding feature point calculated using KLT tracking method in current frame. For the purpose of the performance evaluation, we use wireless-controlled vehicle mounted CCD camera, GPS and INS, and performed the test to calculate the location and the rotation angle of the camera with the video sequence stream obtained at 15Hz frame rate.

• Steel and Composite Structures
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• v.18 no.3
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• pp.583-601
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• 2015

In the present study, the combined effects of thermal and mechanical loadings on the interlaminar shear stresses of both moderately thin and thick composite laminated beams are numerically analyzed. The finite element modelling of laminated composite beams and analysis of interlaminar stresses are performed using the commercially available software package MSC NASTRAN/PATRAN. The validity of the finite element analysis (FEA) is demonstrated by comparing the experimental test results obtained due to mechanical loadings under the influence of thermal environment with those derived using the present FEA. Various parametric studies are also performed to investigate the effect of thermal loading on interlaminar stresses generated in symmetric, anti-symmetric, asymmetric, unidirectional, cross-ply, and balanced composite laminated beams of different stacking sequences with identical mechanical loadings and various boundary conditions. It is shown that the elevated thermal environment lead to higher interlaminar shear stresses varying with the stacking sequence, length to thickness ratio, ply orientations under identical mechanical loading and boundary conditions of the composite laminated beams. It is realized that the magnitude of the interlaminar stresses along xz plane is always much higher than those of along yz plane irrespective of the ply-orientation, length to thickness ratios and boundary conditions of the composite laminated beams. It is also observed that the effect of thermal environment on the interlaminar shear stresses in carbon-epoxy fiber reinforced composite laminated beams are increasing in the order of symmetric cross-ply laminate, unidirectional laminate, asymmetric cross-ply laminate and anti-symmetric laminate. The interlaminar shear stresses are higher in thinner composite laminated beams compared to that in thicker composite laminated beams under all environmental temperatures irrespective of the laminate stacking sequence, ply-orientation and boundary conditions.

• The Korean Journal of Ecology
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• v.25 no.4
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• pp.241-245
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• 2002

During the breeding season of 1998, breeding ecology of the Great Reed-Warbler (Acrocephalus arundinaceus orientalis) was studied at Yangsoo-ri and Yongdam-ri of the Yangpyung-gun, Kyunggi province, Korea. Egg-weight (CV: 6.25) was more variable than either length or breadth, and breadth was the least variable of the measures. Significant variations in overall egg-weight occurred between clutches, and that more of the total variation in egg-weight and shape are due to inter-clutch variation as to intra-clutch variation when the data were pooled. The last egg tends to be larger than the remaining eggs in the clutch of the Great Reed-Warbler, suggesting the Great Reed-Warbler may adopt the brood-survival strategy. When method 3 was used, the most common incubation period is 12 days. In the Great Reed-Warbler, the length of the incubation period was related to clutch-size when method 1 (r=0.485, p<0.05) and method 2 (r=0.621, p<0.01) were employed, but not related to egg weight. The averagee number of days of hatching asynchrony was 2.5, raging 0.5~2.5. Asynchronous hatching was related to the clutch size (r=0.66, p<0.01). Hatching sequence was closely related to the laying sequence (r=0.93, p<0.001), suggesting Great Reed-Warblers incubate their eggs before clutch completion. The effect of egg weight on hatching asynchrony was found in Great Reed-Warblers (t-test, p<0.01).