• Journal of information and communication convergence engineering
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• v.10 no.2
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• pp.210-214
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• 2012

A microtiter well plate DNA hybridization method using Mycobacterium kansasii-specific rpoB DNA probe (kanp) were evaluated for the detection of M. kansasii from culture isolates. Among the 201 isolates tested by this method, 27 strains show positive results for M. kansasii, but the other 174 isolates were negative results for M. kansasii. This result was consistent with partial rpoB sequence analysis of M. kansasii and the result of biochemical tests. The negative strains by this DNA-DNA hybridization method were identified as Mycobacterium tuberculosis (159 strains), Mycobacterium avim (5 strains), Mycobacterium intracellulare (8 strains), and Mycobacterium flavescens (2 strain) by rpoB DNA sequence analysis. Due to high sensitivity and specificity of this test result, we suggest that DNA-DNA hybridization method using rpoB DNA probes of M. kansasii could be used for the rapid and convenient detection of M. kansasii.

• ALGAE
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• v.30 no.4
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• pp.303-312
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• 2015

The intimate physical interaction between food algae and sacoglossan sea slug is a pertinent system to test the theory that “you are what you eat.” Some sacoglossan mollusks ingest and maintain chloroplasts that they acquire from the algae for photosynthesis. The basis of photosynthesis maintenance in these sea slugs was often explained by extensive horizontal gene transfer (HGT) from the food algae to the animal nucleus. Two large-scale expressed sequence tags databases of the green alga Bryopsis plumosa and sea slug Placida dendritica were established using 454 pyrosequencing. Comparison of the transcriptomes showed no possible case of putative HGT, except an actin gene from P. dendritica, designated as PdActin04, which showed 98.9% identity in DNA sequence with the complementary gene from B. plumosa, BpActin03. Highly conserved homologues of this actin gene were found from related green algae, but not in other photosynthetic sea slugs. Phylogenetic analysis showed incongruence between the gene and known organismal phylogenies of the two species. Our data suggest that HGT is not the primary reason underlying the maintenance of short-term kleptoplastidy in Placida dendritica.

• Proceedings of the KSME Conference
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• 2001.11a
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• pp.204-209
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• 2001

In this research, the effects of fiber stacking sequence on damage behaviors of FML(Fiber Metal Laminates) subject to indentation loading. SOP (Singly Oriented Ply) FML and angle ply FML were fabricated to study fiber orientation effects and angle ply effects. FML were fabricated by using 1050 aluminum laminate and carbon/epoxy prepreg. To increase adhesive bonding strength, Al laminate was etched using FPL methods. The static indentation test were conducted by using UTM(5ton, Shimadzu) under the 2side clamped conditions. During the tests, load and displacement curve and crack initiation and propagation behaviors were investigated. As fiber orientation angle increases, the crack initiation load of SOP FML increases because the stiffness induced by fiber orientation is increased. The penetration load of SOP FML is influenced by the deformation tendency and boundary conditions. However, the macro-crack of angle ply FML was initiated by fiber breakage of lower ply because angle plies in Angle ply FML prevents the crack growth and consolidation. The Angle ply FML has a critical cross-angle which prevent crack growth and consolidation. Damage behavior of Angle ply FML is changed around the critical cross-angle.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.23 no.11 s.170
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• pp.1952-1959
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• 1999

The work factor approach, so-called single specimen technique could be used to determine energy release rate from a single test record for unidirectional CLS specimen. In the present study, the work factor approach was extended to determine the mixed-mode fracture toughness of multi-directional graphite/epoxy laminated composites. Multi-directional CLS specimens were used for fracture tests. The stacking sequences used for the lap and the strap were $[90_2/0_2]_s/[0_4/90_4]_s$ and $[0/\pm45/0]_s/[0_2/\pm45_2/0_2]_2$, respectively. For both cases, the fracture toughness determined from the work factor approach was compared with that determined from the compliance method. It was found that both methods produced fracture toughness within a maximum 15% difference for each stacking sequence. The fractography analysis also showed that the fiber bridging occurred for$[0/\pm45/0]_s/[0_2/\pm45_2/0_2]_2$ case while it did not occur for $[90_2/0_2]_s/[0_4/90_4]_s$ case.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.117-117
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• 2017

The onion (Allium cepa L.) is the most widely cultivated species of the genus Allium, especially it has been valued because of the pungent flavor and aroma. Allium species including onion has very large genome sizes ranging from approximately 10 to 20 Gbp, which have complicated genomic studies and precluded genome sequencing until recently. A population of 186 F2 individuals derived from a cross of 'Umjinara' ${\times}$ 'Sinsunhwang' and the two parental lines were used for this study. For the development of framework map, various types of markers including SSRs, RAPD, SNPs, and CAPS makers have been used for polymorphism test. Especially, a lot of SNP and CAPS loci were developed from the onion transcriptome sequence by RNASEQ of two parental lines. The GBS libraries have been constructed based on a modified protocol from Poland Lab using a two-enzyme system. We have been developing markers showing polymorphism between two parental lines, and genotyping for all F2 individuals were finished for a number of polymorphic markers. For the construction of GBS libraries, a set of 192 barcoded adapters were generated from complementary oligonucleotides with XhoI overhang sequence and unique barcodes of length 4-8 bp and they have been tested using two parental linesto determine the optimum conditions for GBS analysis.

• 한국생태학회:학술대회논문집
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• 2002.08a
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• pp.107-111
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• 2002

During the breeding season of 1998, breeding ecology of the Great Reed-Warbler (Acrocephalus arundinaceus orientalis) was studied at Yangsoo-ri and Yongdam-ri of the Yangpyung-gun, Kyunggi province, Korea. Egg-weight (CV: 6.25) was more variable than either length or breadth, and breadth was the least variable of the measures. Significant variations In overall egg-weight occurred between clutches, and that more of the total variation in egg-weight and shape are due to inter-clutch variation as to intra-clutch variation when the data were pooled. The last egg tends to be larger than the remaining eggs in the clutch of the Great Reed-Warbler, suggest- ing the Great Reed-Warbler may adopt the brood-survival strategy. When method 3 was used, the most common incubation period is 12 days. In the Great Reed-Warbler, the length of the incubation period was related to clutch-size when method 1 (r=0.485, p<0.05) and method 2 (r=0.621, p<0.01) were employed, but not related to egg weight. The average number of days of hatching asynchrony was 2.5, raging 0.5∼2.5. Asynchronous hatching was related to the clutch size (r=0.66, p<0.01). Hatching sequence was closely related to the laying sequence (r=0.93, p<0.001), suggesting Great Reed-Warblers incubate their eggs before clutch completion. The effect of egg weight on hatching asynchrony was found in Great Reed-Warblers (t-test, p<0.01).

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.3
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• pp.373-379
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• 2012

This paper presents a fault location algorithm based on the adaptively estimated value of the local sequence source impedance for faults on a parallel transmission line. This algorithm uses only the local voltage and current signals of a faulted circuit. The remote current signals and the zero-sequence current of the healthy adjacent circuit are calculated by using the current distribution factors together with the local terminal currents of the faulted circuit. The current distribution factors consist of local equivalent source impedance and the others such as fault distance, line impedance and remote equivalent source impedance. It means that the values of the current distribution factors can change according to the operation condition of a power system. Consequently, the accuracy of the fault location algorithm is affected by the two values of equivalent source impedances, one is local source impedance and the other is remote source impedance. Nevertheless, only the local equivalent impedance can be estimated in this paper. A series of test results using EMTP simulation data show the effectiveness of the proposed algorithm. The proposed algorithm is valid for a double-circuit transmission line system where the equivalent source impedance changes continuously.

• Journal of Electrical Engineering and Technology
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• v.3 no.4
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• pp.484-490
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• 2008

In this paper, an integrated fault section detection and isolation strategy is proposed based on the application of the Distribution Automation System(DAS) utilizing advanced IT and communication technologies. The Feeder Remote Terminal Unit(FRTU) has been widely used to collect data in the Korean distribution system. The achieved data is adopted in this method for detecting multiple fault types. Especially in the case of single phase-to-ground fault, the fault section is detected by comparison of the zero-sequence current phase angle. The test results have verified the effectiveness of the proposed method in a radial distribution system through extensive simulations in Matlab/Simulink. Furthermore, a communication-based demo system identical to the simulation model has been developed, and it can be applied as an online monitoring and control program for fault section detection and isolation.

• Journal of Korean Academy of Nursing
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• v.37 no.3
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• pp.343-352
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• 2007

Purpose: The purpose of this study was to examine the effects of an inpatient pulmonary rehabilitation program on dyspnea, exercise capacity, and health related quality of life in inpatients with chronic lung disease. Method: This quasi experimental study was designed with a nonequivalent control group pre-post test time series. Twenty three patients were assigned to the experimental group and nineteen to the control group. The inpatient pulmonary rehabilitation program was composed of upper and lower extremity exercise, breathing retraining, inspiratory muscle training, education, relaxation and telephone contacts. This program consisted of 4 sessions with inpatients and 4 weeks at home after discharge. The control group was given a home based pulmonary rehabilitation program at the time of discharge. The outcomes were measured by the Borg score, 6MWD and the Chronic Respiratory Disease Questionnaire(CRQ). Results: There was a statistically significant difference in dyspnea between the experimental group and control group, but not among time sequence, or interaction between groups and time sequence. Also significant improvements in exercise capacity and health related quality of life were found only in the experimental group. Conclusions: An Inpatient pulmonary rehabilitation program may be a useful intervention to reduce dyspnea, and increase exercise capacity and health related quality of life for chronic lung disease patients.

• Journal of Species Research
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• v.4 no.1
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• pp.49-56
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• 2015

Zeuzera is one of the most important wood borer pests in South East Asia. Like of most other genera of moths, the systematic of this genus is still in dispute, especially on the monophyly and the relationship within this genus due to the fact that genus is very varied. This genus was defined based on external characters only such as cross vein Sc-Rs present, humeral plate approximately triangular in shape and anal plate moderately long to moderately short. Therefore, the monophyly of this genus need to be evaluated based on more comprehensive data. To clarify the monophyly of the genus Zeuzera, to reveal the phylogenetic relationships among the Indonesian species, and to establish the genetic characters of Indonesian Zeuzera, we analyzed seven species of Indonesian Zeuzera including three other species distributed around the world based on nucleotide sequence variation across a 580-bp region in the CO I gene. The results showed that the monophyly of Zeuzera was supported by bootstrap tests at the MP and ML tree building methods (>95%). Genus Zeuzera was divided into two groups (A and B) with Z. borneana was excluded from the two groups and occupied at the basal node. Indonesian species was distributed into two different clades. CO I gene alone was able to fully resolve the relationships among species within clade B. However, further investigations were needed by including more species and other genes that the more conserved to test the validity of the phylogenetic hypothesis proposed here.