Journal of information and communication convergence engineering

The Korea Institute of Information and Commucation Engineering (한국정보통신학회)
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Detection of Mycobacterium kansasii Using DNA-DNA Hybridization with rpoB Probe

  • Kweon, Tae-Dong (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Bai, Sun-Joon (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Choi, Chang-Shik (Department of Oriental Medicine Resources, Far East University) ;
  • Hong, Seong-Karp (Division of Bio and Health Sciences, Mokwon University)
  • Received : 2012.04.09
  • Accepted : 2012.05.09
  • Published : 2012.06.30
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Abstract

A microtiter well plate DNA hybridization method using Mycobacterium kansasii-specific rpoB DNA probe (kanp) were evaluated for the detection of M. kansasii from culture isolates. Among the 201 isolates tested by this method, 27 strains show positive results for M. kansasii, but the other 174 isolates were negative results for M. kansasii. This result was consistent with partial rpoB sequence analysis of M. kansasii and the result of biochemical tests. The negative strains by this DNA-DNA hybridization method were identified as Mycobacterium tuberculosis (159 strains), Mycobacterium avim (5 strains), Mycobacterium intracellulare (8 strains), and Mycobacterium flavescens (2 strain) by rpoB DNA sequence analysis. Due to high sensitivity and specificity of this test result, we suggest that DNA-DNA hybridization method using rpoB DNA probes of M. kansasii could be used for the rapid and convenient detection of M. kansasii.

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References

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