• Title/Summary/Keyword: Test Sequence

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Molecular subtyping and antimicrobial susceptibility of Streptococcus dysgalactiae subspecies equisimilis isolates from clinically diseased pigs

  • Oh, Sang-Ik;Kim, Jong Wan;Kim, Jongho;So, Byungjae;Kim, Bumseok;Kim, Ha-Young
    • Journal of Veterinary Science
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    • v.21 no.4
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    • pp.57.1-57.11
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    • 2020
  • Background: Streptococcus dysgalactiae subspecies equisimilis (SDSE) acts as an etiological agent for lameness, neurological signs, and high mortality in pigs. Despite its importance in pig industries and zoonotic potential, little is known about the effects of this pathogen. Objectives: This study aimed to determine the molecular characteristics and antimicrobial resistance of SDSE strains isolated from diseased pigs. Methods: A total 11 SDSE isolates were obtained from diseased pigs. Bacterial identification, PCR for virulence genes, emm typing, and antimicrobial resistance genes, multilocus sequence typing, and antimicrobial susceptibility test were performed. Results: Nine isolates were from piglets, and 8 showed lameness, sudden death, or neurological signs. The isolates were PCR-positive for sla (100%), sagA (100%), and scpA (45.5%), and only 1 isolate amplified the emm gene (stL2764). Eight different sequence types were detected, categorized into 2 clonal complexes and 4 singletons. All the isolates in this study were included in a small cluster, which also contained other strains derived from humans and horses. The minimum inhibitory concentrations for the tested beta-lactams were low, while those for macrolides, tetracyclines, and fluoroquinolones were relatively high. PCR analysis of the macrolide and tetracycline resistance genes demonstrated that the isolates carried erm(B) (18.2%, n = 2), mef(A/E) (9.1%, n = 1), tet(M) (18.2%, n = 2), and tet(O) (90.2%, n = 10). Two isolates presented a mutation in parC, which is associated with fluoroquinolone resistance. Conclusion: This study provided insight into swine-derived SDSE, as it is related to veterinary medicine, and elucidated its zoonotic potential, in the context of molecular epidemiology and antimicrobial resistance in public health.

Test Case Generation of Communication Protocol with Regular Expressions (정규표현식을 이용한 통신 프로토콜의 최소 시험 경로 생성)

  • 김한경
    • Journal of Internet Computing and Services
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    • v.2 no.1
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    • pp.1-11
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    • 2001
  • Though it is proposed to use Petri net or dynamic FSM methods for the generation of test sequences on some specific protocols, those methods ere unavailable on the cases where the protocol allows faults processing or includes paths in looping which cause errors or endless looping by the explosion of states. The determination of test coverage on the protocol software that has been designed and implemented is difficult by the reason of development periods, technical solutions to support and also economical limitations. It is suggested to generate timely protocol software test sequences on the basis of regular expressions covering the functions of protocol. With this regular expression method, the 38 test sequences of Q.2971 protocol has been generated and also minimized the endless looping problem when dynamic test suites are used by simplifying the test path expressions that denotes loops, According to the works, the suggested method is confirmed as simple and easy compare to the other dynamic test sequence generation techniques. Moreover. the method to search an optional test path whether it Is included or not in the regular path expression is reviewed.

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Industrial Fatigue and Low Back Pain of the Workers (산업장 근로자들의 피로자각증상과 요통)

  • Kim, Soon-Lae;Moon, Jung-Soon
    • Research in Community and Public Health Nursing
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    • v.7 no.2
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    • pp.400-409
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    • 1996
  • To investigate industrial fatigue and low back pain, the questionaire survey for subjective symptoms of fatigue and low back pain was carried out among 591 male workers aged 20-55 employed in an automobile industry in Korea. Workers participated to this study were divided into low back pain group(LBP) and control group, according to the self-reports by written questionaires. The subjective sysptoms of fatigue comprised three groups of 10 items each, representing dullness and sleepiness(level of cerebral activation), difficulty in concentration(level of motivation) and bodily projection of fatigue. The resultant data were processed for $\chi^2-test$, t-test and a pearson's correlation coefficient to confirm the relationships. The results were as follows: 1. 30 items of fatigue subjective symptoms exeptone item, 'lack in perseverance', were directly associated with low back pain. 2. The percentage of fatigue complaint were sig nificantly higher in LBP group. 3. Of the 30 items of fatigue subjective symptoms, the highest percentage was accounted for 'eye strain'(27.9%), followed by 'whole body feels tired' and 'legs feel heavy'(22.9%), 'feel like lying'(21.4%), 'feel a pain in the low back'(18.7%), 'feel drowsy'(16.4%) and 'feel stiffness in the neck or the shoulders'(16.2%) in the order of sequence. 4. The average weighted score for the first group of fatigue items(dullness and sleepiness) was the largest among three groups and was followed by the second group(difficulty in concentration) and the third group(bodily projection of fatigue) in the order of sequence, suggesting the heavier shift work stress of the workers. 5. In the groups of the aged 30-40, work duration of 5-7yrs, heavy work amount and irregular work speed, significant high fatigue complaints were revealed in terms of eye strain, whole body feels tired, legs feel heavy, feel like lying, feel a pain in the low back and feel stiffness in the neck or the shoulders. 6. A significant negative correlations were shown between age, work duration and eleven subjective symptoms while positive reciprocal correlations were shown between eleven items with one another.

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A 1.88-mW/Gb/s 5-Gb/s Transmitter with Digital Impedance Calibration and Equalizer (디지털 임피던스 보정과 이퀄라이저를 가진 1.88mW/Gb/s 5Gb/s 송신단)

  • Kim, Ho-Seong;Beak, Seung-Wuk;Jang, Young-Chan
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.20 no.1
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    • pp.110-116
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    • 2016
  • This paper describes 1.2-V 5-Gb/s scalable low voltage signaling(SLVS) differential transmitter(TX) with a digital impedance calibration and equalizer. The proposed transmitter consists of a phase-locked loop(PLL) with 4-phase output clock, a 4-to-1 serializer, a regulator, an output driver, and an equalizer driver for improvement of the signal integrity. A pseudo random bit sequence generator is implemented for a built-in self-test. The proposed SLVS transmitter provides the output differential swing level from 80mV to 500mV. The proposed SLVS transmitter is implemented by using a 65-nm CMOS with a 1.2-V supply. The measured peak-to-peak time jitter of the implemented SLVS TX is about 46.67 ps at the data rate of 5Gb/s. Its power consumption is 1.88 mW/Gb/s.

BEF Detection Algorithm to Improve Reliability of Three-Wire-Unigrounded Distribution Line (3선-단접지배전선로의 신뢰도개선을 위한 BEF 검출 알고리즘)

  • Wan-Ki Min;Myeong-Ho Yoo;Seong-Hwa Kang
    • Journal of the Korean Society of Safety
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    • v.12 no.3
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    • pp.166-172
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    • 1997
  • The BEF on the radial distribution line refers to a class of ground faults in which the load-side power line only is grounded, with the distribution line broken into two parts, the source-side and the load-side. Because its mechanism is remarkably different from that of other earth faults, the fault current is very low, and then difficult to detect the BEF. Thus, it is necessary to analyze its properties and to find an appropriate method that can economically protect the BEF of nonautomation area in the substation. As a result of analyzing the BEF data obtained by the RTDS, EMTP simulation, and the field test data of ETSA, we believe that it is the dominant factor in distinguishing the BEF from normal conditions by a criterion value that is appropriately handled from the zero-sequence current. Thus, with this criterion value, a BEF detecting algorithm is constructed which measures the variations of the zero-sequence current and processes then properly so as to make the fault decision. To prove the accuracy of this algorithm, it is compared with the field test data of ETSA under various conditions. The results show that the proposed algorithm is accurate.

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Isolation of Enterobacter Cloacae Producing Phytase and Medium Optimization of Its Production (Phytase를 생산하는 Enterobacter cloacae의 분리 및 효소 생산의 배지 최적화)

  • 송민동;김영훈;양시용;김대영;김창원;정원형;권문남
    • Microbiology and Biotechnology Letters
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    • v.29 no.2
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    • pp.78-83
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    • 2001
  • Phytase (myo-inositol hexakisphosphate phosphohydrolase: EC 3.1.3.8) hydrolyzes phytic acid (myo-inositol hexakisphosphate) to myo-inositol and monophosphates. In order to obtain phytase producing bacteria, many samples were collected from various soils. Among thirty-five phytase-producing strains, YH100 showed the highest phytase activity. In order to identify the selected YHlOO strain, the morphological and physiological characteristics were examined according to the method of Bergey's manual by 168 rRNA sequence, cellular fatty acids profile, O+C contents and physiological test using API 20E kit. The strain YH100 identified to be a genus of Enterobacter cloacae and was named as Enterobacter cloacae YHlOO. Optimum medium for the phytase production by the Entemhacter c!o([we YHlOO was composed of 2.0%(w/v) glucose, 1.0%(w/v) peptone, 1.0%(w/v) beef extract, 0.1 %(w/v) KCI. and 0.1 %( w/v) sodium phytate.

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Isolation and Characterization of a Novel Aspergillus tubingensis with a Hydrolyzing Activity of Cellulose-pectin Complex (섬유소-펙틴 분해력이 있는 새로운 Aspergillus tubingensis의 분리와 특성 규명)

  • 김영민;서원숙;홍진영;최홍서;김주환
    • Microbiology and Biotechnology Letters
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    • v.31 no.2
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    • pp.124-128
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    • 2003
  • In order to isolate and characterize a novel fungal strain capable of producing cellulase, each samples of the old rice straw, soil, and the old tree were screened by congo red test. One of the fungi screened has been identified as Aspergillus tubingensis strain from the results of the phylogenic analysis based on partial DNA sequence and the basis of its biochemical properties. A carboxymethyl cellulase activity of the strain was higher than that of A. oryzae KCTC 6291. In CMCase activity measurement, it wasn't sensitive about pH 2.0, 3.0, 4.0, but the enzyme was more stable than A. oryzae under the various pH and temperature conditions and the enzyme activity was more similar to neutrality and alkali. Therefore, it could be suggested that the isolated strain has a potential possibility for the developing of the probiotics.

High Speed Serial Link Transmitter Using 4-PAM Signaling (4-PAM signaling을 이용한 high speed serial link transmitter)

  • Jeong, Ji-Kyung;Lee, Jeong-Jun;Burm, Jin-Wook;Jeong, Young-Han
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.46 no.11
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    • pp.84-91
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    • 2009
  • A high speed serial link transmitter using multi-level signaling is proposed. To achieve high data rate m high speed serial link, 4-pulse amplitude modulation (PAM) is used. By transmitting 2 bit data in each symbol time, high speed data transmission, two times than binary signaling, is achieved. The transmitter transmits current-mode output instead of voltage-mode output Current-mode output is much faster than voltage-mode output, so higher data transmission is available by increasing switching speed of driver. $2^5-1$ pseudo-random bit sequence (PRBS) generator is contained to perform built-in self test (BIST). The 4-PAM transmitter is designed in Dongbu HiTek $0.18{\mu}m$ CMOS technology and achieves 8 Gb/s, 160 mV of eye height with 1.8 V supply voltage. The transmitter consumes only 98 mW for 8 Gb/s transmission.

Fracture Behavior of a Stacked Concrete Structure Based on the Fracture Mechanics (적층한 콘크리트 복합구조체의 파괴역학적 거동)

  • Kim, Sang-Chul;Kim, Yeon-Tae
    • Magazine of the Korea Concrete Institute
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    • v.11 no.1
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    • pp.119-127
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    • 1999
  • The objective of this study is to simulate the fracture behavior of composite structure bonded with more than 2 different cementitious materials. For this, concrete and cement were stacked and bonded in a direction perpendicular to loading and specimens were tested. Each constituent material of concrete and cement was fabricated independently also, and three point bending and indirect tensile tests were carried out for the acquisition of measured values applicable to the proposed model. As a result of comparing theoretical results and experimental ones, it was found that the proposed model derived from fictitious crack theory can be used to predict the fracture behavior of composite structures on the vases of well agreement with experimental results. It was also noted that the degree of improvement of fracture energies and strengths is greatly dependent on the stacking sequence of layers composing of a composite structure. Thus, it can be concluded that brittleness or ductility of a composite structure can be accomplished by a proper arrangement of layers on one's purpose throughout the proposed analysis.

A Study on A Multi-Pulse Linear Predictive Filtering And Likelihood Ratio Test with Adaptive Threshold (멀티 펄스에 의한 선형 예측 필터링과 적응 임계값을 갖는 LRT의 연구)

  • Lee, Ki-Yong;Lee, Joo-Hun;Song, Iick-Ho;Ann, Sou-Guil
    • The Journal of the Acoustical Society of Korea
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    • v.10 no.1
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    • pp.20-29
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    • 1991
  • A fundamental assumption in conventional linear predictive coding (LPC) analysis procedure is that the input to an all-pole vocal tract filter is white process. In the case of periodic inputs, however, a pitch bias error is introduced into the conventional LP coefficient. Multi-pulse (MP) LP analysis can reduce this bias, provided that an estimate of the excitation is available. Since the prediction error of conventional LP analysis can be modeled as the sum of an MP excitation sequence and a random noise sequence, we can view extracting MP sequences from the prediction error as a classical detection and estimation problem. In this paper, we propose an algorithm in which the locations and amplitudes of the MP sequences are first obtained by applying a likelihood ratio test (LRT) to the prediction error, and LP coefficients free of pitch bias are then obtained from the MP sequences. To verify the performance enhancement, we iterate the above procedure with adaptive threshold at each step.

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