• Journal of Animal Science and Technology
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• 제51권5호
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• pp.421-426
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• 2009

본 연구는 산란계의 사육공간의 크기별 발성음의 특성을 파악하기 위해 하이라인브라운(Hy-Line Brown) 80주령의 산란계를 대상으로 2008년 10월부터 2009년 2월까지의 기간 동안 발성음을 수집 및 녹음하였다. 산란계의 사육 케이지는 무항생제 축산물 사육밀도조건을 만족하는 0.0231 $m^3$ (0.3m ${\times}$ 0.14m ${\times}$ 0.55m)을 개체당 사육공간의 기준(control)으로 하고, 좁은 공간(small, 0.21m ${\times}$ 0.14m ${\times}$ 0.55m)과 넓은 공간(large, 0.3m ${\times}$ 0.3m ${\times}$ 0.55m) 등의 3가지 유형으로 구분하였다. 하루 3회, 각각 1시간씩 디지털 녹음기(PMD-650, Marantz)와 마이크(MKH 416P48, RF Condenser Mic.)를 이용하여 발성음을 녹음하여 분석을 실시하였다. 발성음의 스펙트로그램을 비교한 결과 케이지의 크기별로 차이가 있는 것으로 나타났으며, 스펙트럼을 비교한 결과 역시 차이가 있었다. 또한 발성음의 주파수, 강도 및 길이 역시 통계적으로 유의한 차이를 나타내었다. 본 연구를 통해 산란계의 사육공간과 발성음 사이에는 깊은 관련이 있었으며, 사육공간에 의한 스트레스의 정도를 파악하기 위해 발성음은 매우 유용할 것으로 판단된다.

• 동의신경정신과학회지
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• 제24권4호
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• pp.419-434
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• 2013

Objectives: The object of this study is to observe the cognition and motor function recovery effects of Joojakwhan (JJW), a traditional Korean poly-herbal formula for treating various neuropsychiatric diseases such as dementia, for the mildly stroke rats, with 60 minutes of reperfusion transient middle cerebral artery occlusion (tMCAO). Methods: In the present study, 125, 250 and 500 mg/kg of JJW were orally administered, once per day for 10 continuous days 2 hours after the tMCAO. The body weight changes, infarct sizes under 2% 2, 3, 5-triphenyl tetrazolium chloride (TTC) stain, sensorimotor functions and cognitive motor behavior tests were serially monitored with cerebral caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP)-immunoreactivities and histopathological changes. The effects of tMCAO on sensorimotor functions were evaluated by using of limb placing and body-swing tests, and the cognitive motor behaviors were also observed with water maze tests. Results: From the results of tMCAO, with marked decreases of body weights, disorders of sensorimotor functions increases the limb placing test scores, and decrease the numbers and percentages of body swings to the ipsilateral sides. The cognitive motor behaviors increases the distances and time to reach the escape platform which included the inhibitions of the decreases with repeated trials that were observed with focal cerebral cortex infarct volumes. In addition, the marked increases of the atrophy, numbers of degeneration, caspase-3- and PARP-immunoreactive cells around peri-infarct ipsilateral cerebral cortex were also observed in tMCAO controls when compared with the sham control rats, respectively. Conclusions: The results obtained from this study suggest that oral administrations of JJW indicate obvious cognitions and motor function recoveries of the rats with tMCAO, mild strokes, which are mediated by neuro-protective effects through known antioxidant effects of components.

• 한국식품과학회지
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• 제45권4호
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• pp.488-494
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• 2013

제2형 당뇨질환모델인 KK-$A^y$를 이용하여 녹차와 발효녹차의 항당뇨 활성을 측정한 결과, 발효 녹차는 비발효녹차에 비해 높은 항당뇨 활성이 있는 것으로 분석되었다. 발효녹차 섭취군의 혈당은 당뇨 대조군보다 낮게 유지되었으며, 60일 이후에는 시판 건강기능식품 섭취군(양성대조군)과 유사한 수준으로 유지되었을 뿐만 아니라 당화혈색소값도 8.08%로 대조군 및 양성대조군 군보다 낮게 나타났다. 간 조직의 DNA microarray 분석결과, 이러한 발효녹차의 항당뇨 활성은 glycolysis 활성화를 통한 glucose 이용율 및 베타세포 function 증가에 의한 것으로 사료된다. 또한 발효녹차는 혈중 triglyceride 수치를 낮추고 HDL-cholesterol 수치를 높이는 등 당뇨로 인해 발생할 수 있는 지질대사이상 개선에도 효과가 있음을 알 수 있었다. 이로 미루어 보아 발효녹차는 항당뇨 관련 건강기능식품으로의 상업적 이용가능성이 높을 것으로 생각된다.

• 한국식품영양과학회지
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• 제43권8호
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• pp.1174-1180
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• 2014

대추와 5개의 균주로 발효시킨 발효대추의 total polyphenol 함량과 in vitro에서의 radical 소거능을 검토한 결과 대추에 비해 발효대추에서의 total polyphenol 함량이 높게 나타났고 DPPH와 OH의 소거능이 우수하였으며, 특히 Saccharomyces cerevisiae로 발효시킨 대추가 가장 우수한 효과를 보였다. 이에 대추 추출물과 Sac. cerevisiae로 발효시킨 대추 추출물을 이용하여 C57BL/6N 탈모 마우스실험 모델 하에서 발모 성장 촉진 효과 및 탈모 예방 효과를 검토하였다. 제모 후 EtOH 도포군에서는 거의 발모가 진행되지 않았으나 Zizyphus jujuba(Zj) 도포군과 Sac. cerevisiae-fermented Zj(Zj-Y) 도포군에서는 전체적으로 발모가 진행되었고, 모발의 성장 속도는 minoxidil 도포군>Zj-Y 도포군> Zj 도포군> EtOH 도포군의 순으로 나타났다. 또한 자발적인 탈모에 대한 예방 효과도 대추, 특히 발효대추 도포군에서 우수함을 관찰할 수 있었다. 본 연구 결과로 대추에 비해 발효대추, 특히 Sac. cerevisiae로 발효시킨 대추가 항산화 효능이 뛰어나며, 발모 촉진 효과 및 탈모예방 효과가 우수함을 알 수 있었다.

• Nutrition Research and Practice
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• 제12권3호
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• pp.191-198
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• 2018

BACKGROUD/OBJECTIVES: Neuroinflammation plays critical role in neurodegenerative disorders, such as Alzheimer's disease (AD). We investigated the effect of three licorice varieties, Glycyrhiza uralensis, G. glabra, and Shinwongam (SW) on a mouse model of inflammation-induced memory and cognitive deficit. MATERIALS/METHODS: C57BL/6 mice were injected with lipopolysaccharide (LPS; 2.5 mg/kg, intraperitoneally) and orally administrated G. uralensis, G. glabra, and SW extract (150 mg/kg/day). SW, a new species of licorice in Korea, was combined with G. uralensis and G. glabra. Behavioral tests, including the T-maze, novel object recognition and Morris water maze, were carried out to assess learning and memory. In addition, the expressions of inflammation-related proteins in brain tissue were measured by western blotting. RESULTS: There was a significant decrease in spatial and objective recognition memory in LPS-induced cognitive impairment group, as measured by the T-maze and novel object recognition test; however, the administration of licorice ameliorated these deficits. In addition, licorice-treated groups exhibited improved learning and memory ability in the Morris water maze. Furthermore, LPS-injected mice had up-regulated pro-inflammatory proteins, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2, interleukin-6, via activation of toll like receptor 4 (TLR4) and nuclear factor-kappa B ($NF{\kappa}B$) pathways in the brain. However, these were attenuated by following administration of the three licorice varieties. Interestingly, the SW-administered group showed greater inhibition of iNOS and TLR4 when compared with the other licorice varieties. Furthermore, there was a significant increase in the expression of brain-derived neurotrophic factor (BDNF) in the brain of LPS-induced cognitively impaired mice that were administered licorice, with the greatest effect following SW treatment. CONCLUSIONS: The three licorice varieties ameliorated the inflammation-induced cognitive dysfunction by down-regulating inflammatory proteins and up-regulating BDNF. These results suggest that licorice, in particular SW, could be potential therapeutic agents against cognitive impairment.

• 한국노년학
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• 제37권3호
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• pp.727-745
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• 2017

본 연구는 중고령층에서 초기노년층의 주관적 건강수준의 변화양상과 그 격차를 결정짓는 요인으로 불안정 고용(precarious employment) 및 고용상태 관련 요인의 영향력을 살펴보고자 하였다. 또한 공적 및 사적 사회적 자본이 중고령기에서 초기노년기에 이르는 주관적 건강상태의 격차를 완화시킬 수 있는지 알아보고자 하였다. 이를 위하여 한국노동패널조사(KLIPS) 6차~17차년도 자료를 사용하였고, 다수준 성장곡선 모형(Multi-level growth curve modeling)을 고정효과(fixed effects) 모형과 확률효과(random effects)모형으로 STATA 13.0을 사용하여 추정하였다. 또한 두 모형을 기반으로 한 가설검정을 위해 하우스만 검정을 수행하였다. 연구결과는 정규직에 비해 일용직이 시간에 따른 주관적 건강상태의 변화를 악화시키는 요인으로 나타났으며, 임금, 고용상태의 질(근로시간), 사적/관계적 사회적 자본이 중고령기에서 초기노년기에 이르는 건강격차를 설명할 수 있는 주요 요인으로 나타났다. 이러한 결과를 기반으로 '확산 가설'과 '누적되는 이익' 가설을 지지하고 있으며, 중고령기에서 초기노년기에 이르는 건강불평등을 완화시킬 수 있는 고용지원 정책 및 사회적 서비스 개발 방향을 제시하고 있다.

• 한국지반공학회논문집
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• 제40권3호
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• pp.77-84
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• 2024

도로 하부의 노후하 된 매설물이 손상되면 공동 및 지반 함몰 등의 재해가 발생할 가능성이 있고 이는 사회적인 비용을 발생시킨다. 본 연구의 목적은 적외선 카메라를 활용하여 공동 위치에 따른 열적 특성을 평가하고 CNN 알고리즘으로 각 공동 위치에 적합한 분류를 수행하고자 하였다. 가로×세로×깊이가 400cm×50cm×40cm인 대상 부지에서 PVC pipe를 상부, 중부 그리고 하부에 매설하여 공동을 조성하였다. 실험부지의 상부에는 포장층을 모사하기 위하여 콘크리트 블록을 설치하였고, 오후 4시부터 다음날 12시까지 측정이 진행되었다. 적외선 카메라로 측정된 초기 온도는 각각 43.7℃, 43.8℃ 그리고 41.9℃로 관측되었고, 측정값은 대기온도 변화가 반영되었다. CNN 알고리즘으로 분류를 진행하기 위해서는 이미지 데이터가 필요하며, 해당 연구에서는 RP 알고리즘을 통해 수치데이터를 이미지로 변환하였다. RP 알고리즘은 4가지 방법을 활용하여 이미지를 생성하였고, 각각의 이미지는 10,000×10,000, 2,000×2,000, 1,000×1,000 그리고 100×100 픽셀로 구성된다. CNN 학습 정확도는 각각 99%, 97%, 98% 그리고 96%로 매우 높게 나타났다. 제안된 방법의 신뢰성은 수치데이터의 정확도와 비교하였으며, 신뢰성이 약 20% 이상 향상된 결과가 나타났다. 이와 같은 결과는 RP 알고리즘으로 전환된 데이터가 적외선 측정된 값의 신뢰성 있는 결과 제공이 가능함을 시사한다.

• 한국제4기학회지
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• 제20권1호
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• pp.51-66
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• 2006

미국 해양대기청의 CCM3 기후모델을 이용하여 마지막최대빙하기 (Last Glacial Maximum (LGM))의 동아시아기후변화를 시뮬레이션 하였다. 이용된 수치모델의 수평해상도는 약 75km로 동아시아의 비교적 상세한 지형 및 기후 기작들이 표현된다. 현재 기후는 미국 해양대기청 에서 제공한 월평균 표층해수가 경계조건으로 이용되었으며, LGM 실험은 CLIMAP 프로젝트에서 복원된 표층해수가 경계조건으로 이용되었다. 또한 LGM실험에서는 현재 의 이산화탄소농도(330 ppm) 보다 낮았던 대기 이산화탄소농도 (200 ppm)가 적용되었고, 대륙빙하를 포함한 LGM 지표지형이 표현되었다. LGM 경계조건하에서 한국 서해와 동해의 대륙사면에서는 표층 온도가 겨울철에 약 18도 정도 현재보다 낮게 나타나며, 여름철에는 약 2도 정도 높게 나타나는데, 이와 같은 온도 변화는 한국 서해가 빙하기때 해수면 하강에 따라 지표로 드러남으로서 표층 열용량의 차이가 나타나기 때문이다. 전체적으로 마지막최대빙하기에 동아시아 내륙에서는 표층온도가 약 4-6도 감소하며, 주변해역을 포함하여 약 7.1도 하강하는 것으로 시뮬레이션 되는데, 이와 같은 온도감소는 이산화탄소농도 감소에 따른 장파복사량의 증가에 기인한다. 표층의 온도감소는 물수지의 약화를 초래하는데, 겨울철 아시아의 남동부에서 강수량이 약 1-4 mm/day 감소하며, 여름철에는 중국에서의 감수감소가 나타난다. 대체로 마지막최대 빙하기동안 동북아시아에서 현재보다 약 50%정도 감수가 감소하는 것으로 시뮬레이션 된다. 증발량도 감소하는 것으로 나타나지만, 강수의 감소가 더 커서 지난빙하기동안 동아시아는 대체로 건조한 기후가 나타나며, 이와 같은 결과는 다른 지역에서 보이고 있는 결과와 잘 일치한다. 결론적으로 고해상도 수치모델은 전 지구 영역 하에서 동아시아의 기후변화를 잘 시뮬레이션 하고 있다.mu}M$에서 $9.1{\mu}M$까지, 알테미아 경우 $16.13{\mu}M$에서 $45.8{\mu}M$까지, 그리고 배합 사료 공급시에는 $36.5{\mu}M$에서 $120.1{\mu}M$까지 상승하였다. 일간 수질 변화에 따른 용존 무기 질소(로티퍼; 7.0 g/일, 알테미아; 24.7 g/일, 배합 사료; 140.9 g/일)와 인(로티퍼; 0.7 g/일, 알테미아; 0.7 g/일, 배합 사료; 2.2 g/일) 배출량은 배합 사료 공급 시기에 유의적으로 높았다(P<0.05). 이와 같은 결과는 상업적 볼락 대량 종묘 시설에서 사육 시스템 내 수질 및 사육 관리를 위한 중요한 정보를 제공할 것이다.였다. 2종의 박하 추출물 투여군들 사이에는 혈청 GPT 활성에 대한 유의적인 차이가 나타나지 않았다. 7.과산화물질 (TBARS)의 함량은 정상군에 비해 음성대조군에서 약간의 함량 증가가 관찰되었으나 유의적인 수준은 아니었으며 대부분의 박하 추출물 투여군과 Silymarin 투여군에서 과산화지질이 비슷한 수준으로 증가하여 박하는 알콜에 의한 과간화지질생성에 대한 저해효과가 낮은 것으로 확인되었다. 8. 상대간장중량은 각 실험군별로 상대간장중량에서의 차이는 다소 있었으나 유의성은 없었다.mg/ml로 한 EtOAc 분획물은 26의 돌연변이 유발성을 보였다. 이상의 결과로 부터 뽕나무를 이용한 식용 제품생산을 고려할 때 추출물들의 제조와 선택을 가름하는 자료로서의 활용이 기대되며 앞으로 in vivo test 등 더욱 연구할 필요가 있을 것으로 사료된다.0^{-4}\;pg/cell$로 60 kHz로 병행 추출한 복분자 water 분획층의 $19.5{\times}10^{-4}\;pg/cell$보다 높은 분비량을 나타내었다.

• 대한간호학회지
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• 제12권2호
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• pp.1-26
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• 1982

The main purpose of this study was to test the effect of the structured information on the sleep amount of the patients undergoing open heart surgery. This study has specifically addressed to the Following two basic research questions: (1) Would the structed in formation influence in the reduction of sleep disturbance related to anxiety and Physical stress before and after the operation? and (2) that would be the effects of the structured information on the level of preoperative state anxiety, the hormonal change, and the degree of behavioral change in the patients undergoing an open heart surgery? A Quasi-experimental research was designed to answer these questions with one experimental group and one control group. Subjects in both groups were matched as closely as possible to avoid the effect of the differences inherent to the group characteristics, Baseline data were also. collected on both groups for 7 days prior to the experiment and found that subjects in both groups had comparable sleep patterns, trait anxiety, hormonal levels and behavioral level. A structured information as an experimental input was given to the subjects in the experimental group only. Data were collected and compared between the experimental group and the control group on the sleep amount of the consecutive pre and post operative days, on preoperative state anxiety level, and on hormonal and behavioral changes. To test the effectiveness of the structured information, two main hypotheses and three sub-hypotheses were formulated as follows; Main hypothesis 1: Experimental group which received structured information will have more sleep amount than control group without structured information in the night before the open heart surgery. Main hypothesis 2: Experimental group with structured information will have more sleep, amount than control group without structured information during the week following the open heart surgery Sub-hypothesis 1: Experimental group with structured information will be lower in the level of State anxiety than control group without structured information in the night before the open heart surgery. Sub-hypothesis 2 : Experimental group with structured information will have lower hormonal level than control group without stuctured information on the 5th day after the open heart surgery Sub-hypothesis 3: Experimental group with structured information will be lower in the behavioral change level than control group without structured information during the week after the open heart surgery. The research was conducted in a national university hospital in Seoul, Korea. The 53 Subjects who participated in the study were systematically divided into experimental group and control group which was decided by random sampling method. Among 53 subjects, 26 were placed in the experimental group and 27 in the control group. Instruments; (1) Structed information: Structured information as an independent variable was constructed by the researcher on the basis of Roy's adaptation model consisting of physiologic needs, self-concept, role function and interdependence needs as related to the sleep and of operational procedures. (2) Sleep amount measure: Sleep amount as main dependent variable was measured by trained nurses through observation on the basis of the established criteria, such as closed or open eyes, regular or irregular respiration, body movement, posture, responses to the light and question, facial expressions and self report after sleep. (3) State anxiety measure: State Anxiety as a sub-dependent variable was measured by Spi-elberger's STAI Anxiety scale, (4) Hormornal change measure: Hormone as a sub-dependent variable was measured by the cortisol level in plasma. (5) Behavior change measure: Behavior as a sub-dependent variable was measured by the Behavior and Mood Rating Scale by Wyatt. The data were collected over a period of four months, from June to October 1981, after the pretest period of two months. For the analysis of the data and test for the hypotheses, the t-test with mean differences and analysis of covariance was used. The result of the test for instruments show as follows: (1) STAI measurement for trait and state anxiety as analyzed by Cronbachs alpha coefficient analysis for item analysis and reliability showed the reliability level at r= .90 r= .91 respectively. (2) Behavior and Mood Rating Scale measurement was analyzed by means of Principal Component Analysis technique. Seven factors retained were anger, anxiety, hyperactivity, depression, bizarre behavior, suspicious behavior and emotional withdrawal. Cumulative percentage of each factor was 71.3%. The result of the test for hypotheses show as follows; (1) Main hypothesis, was not supported. The experimental group has 282 minutes of sleep as compared to the 255 minutes of sleep by the control group. Thus the sleep amount was higher in experimental group than in control group, however, the difference was not statistically significant at .05 level. (2) Main hypothesis 2 was not supported. The mean sleep amount of the experimental group and control group were 297 minutes and 278 minutes respectively Therefore, the experimental group had more sleep amount as compared to the control group, however, the difference was not statistically significant at .05 level. Thus, the main hypothesis 2 was not supported. (3) Sub-hypothesis 1 was not supported. The mean state anxiety of the experimental group and control group were 42.3, 43.9 in scores. Thus, the experimental group had slightly lower state anxiety level than control group, howe-ver, the difference was not statistically significant at .05 level. (4) Sub-hypothesis 2 was not supported. . The mean hormonal level of the experimental group and control group were 338 ㎍ and 440 ㎍ respectively. Thus, the experimental group showed decreased hormonal level than the control group, however, the difference was not statistically significant at .05 level. (5) Sub-hypothesis 3 was supported. The mean behavioral level of the experimental group and control group were 29.60 and 32.00 respectively in score. Thus, the experimental group showed lower behavioral change level than the control group. The difference was statistically significant at .05 level. In summary, the structured information did not influence the sleep amount, state anxiety or hormonal level of the subjects undergoing an open heart surgery at a statistically significant level, however, it showed a definite trends in their relationships, not least to mention its significant effect shown on behavioral change level. It can further be speculated that a great degree of individual differences in the variables such as sleep amount, state anxiety and fluctuation in hormonal level may partly be responsible for the statistical insensitivity to the experimentation.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제28권5호
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• pp.375-395
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• 2006

Purpose: Lingual nerve (LN) damage may be caused by either tumor resection or injury such as wisdom tooth extraction, Although autologous nerve graft is sometimes used to repair the damaged nerve, it has the disadvantage of necessity of another operation for nerve harvesting. Moreover, the results of nerve grafting is not satisfactory. The nerve growth factor (NGF) is well-known to play a critical role in peripheral nerve regeneration and its local delivery to the injured nerve has been continuously tried to enhance nerve regeneration. However, its application has limitations like repeated administration due to short half life of 30 minutes and an in vivo delivery model must allow for direct and local delivery. The aim of this study was to construct a well-functioning $rhNGF-{\beta}$ adenovirus for the ultimate development of improved method to promote peripheral nerve regeneration with enhanced and extended secretion of hNGF from the injured nerve by injecting $rhNGF-{\beta}$ gene directly into crush-injured LN in rat model. Materials and Methods: $hNGF-{\beta}$ gene was prepared from fetal brain cDNA library and cloned into E1/E3 deleted adenoviral vector which contains green fluorescence protein (GFP) gene as a reporter. After large scale production and purification of $rhNGF-{\beta}$ adenovirus, transfection efficiency and its expression at various cells (primary cultured Schwann cells, HEK293 cells, Schwann cell lines, NIH3T3 and CRH cells) were evaluated by fluorescent microscopy, RT-PCR, ELISA, immunocytochemistry. Furthermore, the function of rhNGF-beta, which was secreted from various cells infected with $rhNGF-{\beta}$ adenovirus, was evaluated using neuritogenesis of PC-12 cells. For in vivo evaluation of efficacy of $rhNGF-{\beta}$ adenovirus, the LNs of 8-week old rats were exposed and crush-injured with a small hemostat for 10 seconds. After the injury, $rhNGF-{\beta}$ adenovirus($2{\mu}l,\;1.5{\times}10^{11}pfu$) or saline was administered into the crushed site in the experimental (n=24) and the control group (n=24), respectively. Sham operation of another group of rats (n=9) was performed without administration of either saline or adenovirus. The taste recovery and the change of fungiform papilla were studied at 1, 2, 3 and 4 weeks. Each of the 6 animals was tested with different solutions (0.1M NaCl, 0.1M sucrose, 0.01M QHCl, or 0.01M HCl) by two-bottle test paradigm and the number of papilla was counted using SEM picture of tongue dorsum. LN was explored at the same interval as taste study and evaluated electro-physiologically (peak voltage and nerve conduction velocity) and histomorphometrically (axon count, myelin thickness). Results: The recombinant adenovirus vector carrying $rhNGF-{\beta}$ was constructed and confirmed by restriction endonuclease analysis and DNA sequence analysis. GFP expression was observed in 90% of $rhNGF-{\beta}$ adenovirus infected cells compared with uninfected cells. Total mRNA isolated from $rhNGF-{\beta}$ adenovirus infected cells showed strong RT-PCR band, however uninfected or LacZ recombinant adenovirus infected cells did not. NGF quantification by ELISA showed a maximal release of $18865.4{\pm}310.9pg/ml$ NGF at the 4th day and stably continued till 14 days by $rhNGF-{\beta}$ adenovirus infected Schwann cells. PC-12 cells exposed to media with $rhNGF-{\beta}$ adenovirus infected Schwann cell revealed at the same level of neurite-extension as the commercial NGF did. $rhNGF-{\beta}$ adenovirus injected experimental groups in comparison to the control group exhibited different taste preference ratio. Salty, sweet and sour taste preference ratio were significantly different after 2 weeks from the beginning of the experiment, which were similar to the sham group, but not to the control group.