• Title/Summary/Keyword: Test 1.1 & 2.1

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Detection of IgG and IsM Antibodies with Immunofluoreseent Antibody Technique in Buman Trichomoniasis (질트리코모나스증에서 간접형광항체법을 이용한 혈청내 항질트리코모나스 IgG 및 IgM 항체의 측정)

  • 윤경찬;김경민;안명희;민득영;차동수
    • Parasites, Hosts and Diseases
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    • v.25 no.1
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    • pp.7-12
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    • 1987
  • The indirect fluorescent antibody(IFA) test was used to detect serum IgG and IgM antibodies to Trichomonas vaginalis in 31 vaginal trichomoniasis, 7 candidiasis and in 20 non-infected healthy women with antigen prepared from axonic culture of Trichomenas vaginalis isolated from vulvovaginitis patient. The results were as follows: 1. In 31 vaginal trichomoniasis the positive reactions of IgG antibody were 27 in the 1/8 dilution or higher and :l in the 1/4 dilution whereas in healthy women the reaction showed significantly low as in the 1/4 dilution or below. 2. The sensitivity and specificity of IFA test for IgG antibody to trichomonad antigen in this study were 87.1% and 100%, respectively. 3. No significant difference of IgM antibody levels between vaginal trichomoniasis and healthy women was observed. 4. No relation between the levels of IgG and IsM antibodies to trichomonad antigen by IFA test was observed. 5. No relation between the time lapse and the level of serum IgG antibodies in IFA test of vaginal trichomoniasis was regarded. In conclusion the present study suggests that IFA test in trichomoniasis could be a useful tool for detection of anti-trichomonad IgG antibodies and applicable as an immunodiagnostic method.

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Synthesis of DL-Aminoalkyl Phosphonic Acids and Their Derivatives (III) (DL-Aminoalkyl Phosphonic Acid와 그 유도체들의 합성 (제3보))

  • Man Khyun Rho;Yong Joon Kim
    • Journal of the Korean Chemical Society
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    • v.17 no.2
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    • pp.136-141
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    • 1973
  • DL-1-aminobutylphosphonic acid was synthesized from the pentanoic acid which was prepared from the butyl alcohol, by the modified Curtius reaction. DL-2-amino-2-carboxyethylphosphonic acid was also synthesized from the pyruvic acid was also synthesized from the pyruvic acid. Four previously unreported N-acylated derivatives were prepared according to the modified Schotten-Baumann method. They are as follows; N-acetyl-DL-1-aminobutylphosphonic acid, N-benzoyl-DL-1-aminobutylphosphonic acid, N-benzoyl-DL-2-amino-2-carboxyethylphosphonic acid, N-p-chlorobenzoyl-DL-2-amino-2-carboxyethylphosphonic acid. The products were identified by the methods of elemental analysis, infrared spectra, ninhydrin test and neutralization equivalent.

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Generalized Divisibility Rule of Natural Number m (자연수 m의 일반화된 배수 판정법)

  • Lee, Sang-Un
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.14 no.5
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    • pp.87-93
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    • 2014
  • For n/m=qm+r, there is no simple divisibility rule for simple m=7 such that is the n multiply by m? This problem can be more complex for two or more digits of m. The Dunkels method has been known for generalized divisibility test method, but this method can not compute very large digits number that can not processed by computer. This paper suggests simple and exact divisibility method for m completely irrelevant n and m of digits. The proposed method sets $r_1=n_1n_2{\cdots}n_l(mod m)$ for $n=n_1n_2n_3{\cdots}n_k$, $m=m_1m_2{\cdots}m_l$. Then this method computes $r_i=r_{i-1}{\times}10+n_i(mod m)$, $i=2,3,{\cdots}k-l+1$ and reduces the digits of n one-by-one. The proposed method can be get the quotient and remainder with easy, fast and correct for various n,m experimental data.

Development and Flight Test of Educational Water Rocket CULV-1 for Implementation of Launch Vehicle Separation Sequence and Imaging Data Acquisition (발사체 분리과정모사 및 단계별 영상획득이 가능한 교육용 물로켓 CULV-1 개발 및 비행시험)

  • Lee, Myeongjae;Park, Taeyong;Kang, Soojin;Jang, Sueun;Oh, Hyunung
    • Journal of Aerospace System Engineering
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    • v.10 no.2
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    • pp.14-21
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    • 2016
  • In this study, we proposed a water rocket CULV-1 (Chosun University Launch Vehicle-1). Unlike a conventional water rocket, CULV-1 can perform the booster rocket, fairing, and payload separation like an actual launch vehicle and also the imaging data acquisition. The conceptual and critical design of the proposed CULV-1 have been performed considering the operation characteristics. The verification tests have been performed from subsystem to system level in accordance with the established test specifications and verification procedures. Through the final launch test of the flight model, we have verified the design effectiveness of the proposed separation mechanisms for water rocket applications and the mission requirements of the CULV-1 also have been complied.

Serological Diagnosis for Swine Cysticercus Cellulosae Infection in Korea (돼지의 유구낭충(有鉤囊蟲)에 관(關)한 혈청학적시험(血淸學的試驗))

  • Jean, Y.;Lee, T.J.
    • Korean Journal of Veterinary Research
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    • v.25 no.1
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    • pp.77-89
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    • 1985
  • The agar gel precipitation(AGP), indirect hemagglutination(IHA) and indirect enzyme immunoassay(IEIA) tests were used to detect antibodies in pigs naturally infected with Cysticercus cellulosae in Jeju. The results obtained were summarized as follows: 1. Sera collected from pigs naturally infected with Cysticcrcus cellulosae did not react in AGP test. 2. In the IHA test for swine cysticercosis, the peak titers observed were between 1:20 and 1:160 and non-specific reaction was recognized with a few samples among control sera. 3. In the IEIA test, optical density(OD) values were obtained the best results under the condition of OPD-substrats are reacted at room temperature for 5minutes. The OD values of greater than 0.2 were determined as positive and the high titers in positive sera ranged from 1:40 to 1:1,080. 4. Antibodies to swine cysticercosis were detected by IHA and IEIA tests but the latter was more sensitive and specific than the former. 5. In the preparation of Cystisercus antigens, saline extract which was prepared the precipitate of internal membrane treated ultrasonicator were better results than other antigens for serological tests. 6. Amounts of protein in antigens was not related in direct proportion to results of serological reaction.

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A Study of the Behavioral Characteristics of the Primary and Secondary Searches on Online Databases (온라인 데이터베이스의 1차탐색과 2차탐색의 특성 연구)

  • Noh Dong-Jo
    • Journal of the Korean Society for Library and Information Science
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    • v.32 no.2
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    • pp.189-209
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    • 1998
  • The purpose of this study is to verify the difference of behavioral characteristics between the primary and secondary search, the influence of the primary search results on Online databases. Data is collected by surveying the professional and end-user searchers in 33 online information service centers. 262 valid responses out of 308 questionaire are analyzed by the t-test, ANOVA, $\chi^2-test$ using SAS program. The major findings are as follows : (1) Preparatory searches(the levels of expectation, the degree of question comprehension), search results(the number of output documents, precision ratio, recall ratio, degree of satisfaction) are significantly different between the primary and secondary searches on Online Databases. But the number of search terms, Boolean logics, files, systems, relevant documents are not significantly different between the primary and secondary searches. (2) The results of the primary search affect the secondary searches. The number of output documents in the primary search affect the modification of search strategies and objects in the secondary searches. The number of relevant documents affect the variation of search scopes in the secondary searches. The precision ratio affect the change of search strategies and scopes in the secondary searches.

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Isolation and Identification of Enterococcus faecalis 2B4-1 Containing Antitumor Substances. (항종양활성을 지닌 Enterococcus faecalis 2B4-1의 분리 및 동정)

  • 박상진;임대석;윤상군;백영진;김창한
    • Microbiology and Biotechnology Letters
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    • v.26 no.6
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    • pp.471-475
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    • 1998
  • The aim of the present research program was to develop a strain of gastrointestinal bacteria containing antitumor substances. Fecal samples were collected from neonates and a number of gastrointestinal bacteria were isolated from the fecal samples by applying selective agar for intestinal bacteria. Among 127 isolates, a strain 2B4-1 containing an antitumor substance against stomach cancer, SNU-1, was selected. The strain 2B4-1 was identified as a strain similar to Enterococcus faecalis NCTC 775 with respect to morphological characteristics, growth temperature, salt and acid tolerance, growth under facultative anaerobic conditions and utilization of carbon sources such as arabinose and melibiose and so on. However, it showed some differences such as a negative reaction to hippurate hydrolysis and negative reaction to $\beta$-hemolysis. We assigned to the strain 2B4-1 to Enterococcus faecalis.

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Eye Irritation Test of Soyeom Pharmacopuncture Solution for Eye Drop (점안용 소염약침액의 안점막자극실험)

  • Seo, Hyeong-Sik
    • Journal of Pharmacopuncture
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    • v.11 no.2
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    • pp.75-79
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    • 2008
  • Objectives This study was done to investigate the safety of Soyeom pharmacopuncture solution manufactured with freezing dryness method to use eye drop. Methods The eye irritation test of this material was performed according to the Regulation of Korea Food & Drug Administration(2005. 10. 21, KFDA 2005-60). After Soyeom pharmacopuncture solution was medicated in the left eye of the rabbits, the auther observed eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Results 1. After Soyeom pharmacopuncture solution was medicated in the left eye of the rabbits, there wasn't physical problem at 9 rabbits. 2. After Soyeom pharmacopuncture solution was medicated in the left eye of the rabbits, there wasn't eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Conclusions I suggested that Soyeom pharmacopuncture solution didn't induced eye irritation in rabbits.

Eye Irritation Test of Bovis Calculus Pharmacopuncture Solutions for Eye Drop (점안용 우황약침액의 안점막자극실험)

  • Seo, Hyeong-Sik
    • Journal of Pharmacopuncture
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    • v.11 no.2
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    • pp.81-85
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    • 2008
  • Objective This study was done to investigate the safety of Bovis Calculus pharmacopuncture solution manufactured with freezing dryness method to use eye drop. Methods The eye irritation test of this material was performed according to the Regulation of Korea Food & Drug Administration (2005. 10. 21, KFDA 2005-60). After Bovis Calculus pharmacopuncture solution was medicated in the left eye of the rabbits, the auther observed eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Results 1. After Bovis Calculus pharmacopuncture solution was medicated in the left eye of the rabbits, there wasn't physical problem at 9 rabbits. 2. After Bovis Calculus pharmacopuncture solutionwas medicated in the left eye of the rabbits, there wasn't eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Conclusions I suggested that Bovis Calculus pharmacopuncture solution didn't induced eye irritation in rabbits.

Seroprevalence of Dirofilaria immitis infections in Jindo dogs (진돗개의 심장사상충 감염 실태 조사)

  • Kim, Jong-Seok;Lee, Jeong-Chi
    • Korean Journal of Veterinary Service
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    • v.41 no.3
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    • pp.179-183
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    • 2018
  • A survey on the seroprevalence of Dirofilaria immitis infection among 2,197 Jindo dogs (371 males, 1,826 females) was conducted from 2011 to 2015 using an antigen test (SNAP 4Dx plus, IDEXX, USA). The overall seroprevalence of D. immitis infection in Jindo dogs was 21.8% (479/2,197), and an increase was observed in the seroprevalence of infection from 19.4% in 2011 to 25.8% in 2015. The infection rates were higher among male dogs (32.1%, 119/371) than female dogs (19.7%, 360/1,826)(P<0.01). The infection rates of D. immitis in Jindo dogs at the age of under 1 years-old, 1 to 2 years-old, 2 to 3 years-old and over 3 years old were 10.7% (76/710), 20.0% (190/950), 36.4% (156/428) and 52.3% (57/109), respectively. The older dogs had higher infection rates than the younger dogs (P<0.01). This study indicates that the prevalence rate of D. immitis in Jindo dogs is higher than previously reported in studies which utilized an antigen test.