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Electrophoretical Analysis of 36-Kilodalton Outer Membrane Protein of Vibrio vulnificus ATCC 27562

  • Moon-Soo Heo;Cho-Rok Jung
    • Journal of Life Science
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    • v.9 no.1
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    • pp.35-39
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    • 1999
  • Elecrophoreticl analysis of a 36 kDa protein was runned by SDS-PAGE, isoelectric focusing (IEF) and two dimensional electrophoresis pattern. Major 36 kDa and 25, 46, 48, 66 kDa protein were detected by Coomassie blue stain on SDS-PAGE. Major 36kDa protein was eluted for production of antiserum for serological analysis, IEF and two dimensional electrophoresis. Isoelectric point of 36kDa was aout pH 8.5. Two dimensional electrophoresis of eluted 36kDa showed one point on the gel. Anti-36 kDa serum made by newzilland rabbit for serological test. In ELISA, final titer of antibody was 100×{TEX}$2^5}${/TEX} : 1. Neutralize ability of serum was examined by slide agglutination test and colonization test in rat. Anti-36 kDa serum agglutinated whole cell of V. vulnificus were inhibited colonization on intestine in rat. Accordingly In this paper contain some electrophoretical analysis and serological test of a 36 kDa OMP of V. vulnificus.

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A Study on the Dynamic Characteristics of AirshipThrough the Flight Test

  • Woo, Gui-Aee;Kim, Jong-Kwon;Cho, Kyeum-Rae;Lee, Dae-Woo
    • International Journal of Aeronautical and Space Sciences
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    • v.5 no.1
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    • pp.75-82
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    • 2004
  • Nowadays, many kinds of research for airship have being studied withincrease of interests in airship. But these make little progress even now. In flighttest, the data acquisition from the actual flight test has lots of difficulties becausethe airship dynamic response is slow and sensitive to extemal environment. In thispaper, through the actual flight test, appropriateness of the mathematical dynamicmodel was presented by showing the test results in various conditions. Thetuming, the acceleration, and the deceleration motions were tested and analyzed.

A Study on the Minimal Test Pattern of the RAM (RAM의 최소 테스트 패턴에 관한 연구)

  • 김철운;정우성;김태성
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1996.11a
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    • pp.23-25
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    • 1996
  • In this paper aims at studying the minimal test pattem of the RAM. This also propose a scheme of testing faults from the new fault model using the LLB. The length of test patterns are 6N(1-wsf), 9.5N(2-wsf), 7N(3-wsfl, 3N(4-wsf) operations in N-bit RAM. This test techniques can write into memory cell the number of write operations is reduced and then much testing time is saved. A test set which detects all positive-negative static t-ws faults for t=0, 1, 2, 3, 4 and detects all pattern sensitive fault in memory array. A new fault model, which encompasses the existing fault model Is proposed.

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On Testing Exponentiality Against HNRBUE Based on Goodness of Fit

  • Mahmoud, M.A.W.;Diab, L.S.
    • International Journal of Reliability and Applications
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    • v.8 no.1
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    • pp.27-39
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    • 2007
  • Based on goodness of fit new testing procedures are derived for testing exponentiality against harmonic new renewal better than used in expectation (HNRBUE). For this aging properties, a nonparametric procedure (U-statistic) is proposed. The percentiles of this test statistic are tabulated for sample sizes n=5(1)30(10)50. The Pitman asymptotic efficiency (PAE) of the test is calculated and compared with, the (PAE) of the test for new renewal better than used (NRBU) class of life distribution [see Mahmoud et al (2003)]. The power of this test is also calculated for some commonly used life distributions in reliability. The right censored data case is also studied. Finally, real examples are given to elucidate the use of the proposed test statistic in the reliability analysis.

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The flexural of Ultra High Strength Reinforced Concrete Beams (초고강고 RC보의 휨거동)

  • 정철규;조인철;안종문;이광수;신성우
    • Proceedings of the Korea Concrete Institute Conference
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    • 1997.04a
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    • pp.475-482
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    • 1997
  • This study reports the results of flexural test on 6 specimens for maximum reinforcement ratio and 4 specimens for minmum reinforcement ratio with concrete compressive strength 1,000, 1,200 kg/$\textrm{cm}^2$ . The major test variables for the reinforcement ratio designed 0.55$\rho_b$, 0.65$\rho_b$, 0.75$\rho_b$ for maximurm reinforcement ratio test 14/fy $\sqrt[0.72]{\rho}\acute{f}_c$ / $f_y$ for minimum reinforcement ratio test. The test results were compared with ACI 318-95 Code. In the Ultra High Strength Concrete beam, the maximum reinforcement ration should be less than 0.6$\rho_b$ for ductile behavior and the existing minimum reinforcement ratio by ACI Building Code is Sufficiently safe.

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A study on the sled test methods for IIHS small overlap performance development (IIHS small overlap 성능개발을 위한 대차 시험 방법 연구)

  • Oh, Hyungjooon;Kim, Seungki;Kim, Sungwon;Lim, Kyungho
    • Journal of Auto-vehicle Safety Association
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    • v.5 no.1
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    • pp.11-15
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    • 2013
  • Small overlap crash caused fatal injury in real-world crash. IIHS(Insurance Institute for Highway Safety) proposed the small overlap test. The objective of this study is to analyze dummy injury criteria and dummy excursion on the sled reinforced body angle. Result of the comparisons of dummy injury criteria of a head, neck, and chest was best correlation between sled and vehicle test on base $angle+3^{\circ}$. However, lower extremity was not correlation because sled test could not copy of intrusion. There were a correlation between dummy movement and sled reinforced body angle. Sled reinforced body angle affects the lateral direction of excursion more than longitudinal excursion.

FRP or steel plate-to-concrete bonded joints: Effect of test methods on experimental bond strength

  • Chen, J.F.;Yang, Z.J.;Holt, G.D.
    • Steel and Composite Structures
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    • v.1 no.2
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    • pp.231-244
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    • 2001
  • The strengthening of reinforced concrete structures using externally bonded steel or advanced fibre reinforced plastic (FRP) composites is becoming increasingly common. A key factor affecting the behaviour and reliability of such strengthened structures is the bond strength between the steel or FRP plate and the concrete substrate. Several different experimental set-ups have previously been used to determine bond strength. This paper presents a careful finite element analysis of the stress distributions in these test set-ups. Results show that stress distributions can be significantly different for different set-ups, for similar materials and geometry.

A safety evaluation on the loading test of EMU′s carbody having stainless and aluminum (도시철도차량의 스테인리스와 알루미늄 구조체 하중시험에 대한 안전성평가)

  • 정종덕;김원경;홍용기;윤성철;이장욱
    • Proceedings of the KSR Conference
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    • 2002.10a
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    • pp.156-161
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    • 2002
  • This paper describes the result of carbody load test. The purpose of the test is to evaluate an safety which carbody structure shall be considered fully sufficient rigidity so as to satisfy proper system function under maximum load and operating condition. Carbody material applied a stainless steel and an aluminum alloy, The stainless steel model is the carbody of a motor car which is delivering to KNR line 1 in 2002 and the aluminum alloy model is the carbody of a motor car which is delivering to GWANGJU line 1 in 2003. This strength test is based on "performance test standard for Electrical Multiple Unit, noticed by Ministry of Construction & Transportation, in 2000" and reference code is UIC 566 and JIS E 7105.

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Biaxial Fracture Behavior of Alumina Ceramics : Indentation Effect on Ball-on-3-ball Test (압입에 따른 알루미나 세라믹스의 이축 파괴 거동)

  • 정성민;박성은;이홍림
    • Journal of the Korean Ceramic Society
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    • v.37 no.7
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    • pp.713-720
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    • 2000
  • The biaxial fracture behavior of alumina ceramics was studied using ball-on-3-ball test. The polished surfaces of alumina specimens were indented at 0mm, 1mm, 2mm, 3mm apart from the center of the specimen along path A, passing between the two supporting balls from the center of the specimen, and along path B, passing above the three supporting balls from the center of the specimen. The fracture strength of the indented specimens was measured using the ball-on-3-ball test, a kind of biaxial strength test. The fracture strength increased with increasing the distance from the center to indented position. The fracture strength of the specimen indented along path B was higher than that of the specimens indented along path A. It was presented that the fracture caused by tangential stress rather than radial stress when the indented positions are 1mm and 2mm from the center of the specimen. This phenomenon was in good agreement with FEM analysis.

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Plant Minimum Stable Load (Pmin) Test for Ilijan CCPP

  • Kim, Si Moon;Yun, Wan No;Jang, Cheol Ho;Park, Se Ik
    • KEPCO Journal on Electric Power and Energy
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    • v.2 no.1
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    • pp.83-88
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    • 2016
  • This paper describes the test results of plant minimum stable load (Pmin) for Ilijan Power Plant. The test was conducted on May 13 through 14, 2015 to investigate the plant operating and equipment condition in accordance with "Ilijan Plant Performance Test Procedure on Plant Minimum Stable Load" [1]. This paper also contains the assessment of the impact of Pmin to plant operating parameters and possible technical operating issues when operating at lower loads and to recommend the safe minimum load operation of Ilijan per block. In addition, this paper describes the performance calculation results of efficiency and heat rate depending on the load level.