• Genomics & Informatics
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• v.21 no.1
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• pp.3.1-3.10
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• 2023

Characterization as well as prediction of the secondary and tertiary structure of hypothetical proteins from their amino acid sequences uploaded in databases by in silico approach are the critical issues in computational biology. Severe acute respiratory syndrome-associated coronavirus (SARS-CoV), which is responsible for pneumonia alike diseases, possesses a wide range of proteins of which many are still uncharacterized. The current study was conducted to reveal the physicochemical characteristics and structures of an uncharacterized protein Q6S8D9_SARS of SARS-CoV. Following the common flowchart of characterizing a hypothetical protein, several sophisticated computerized tools e.g., ExPASy Protparam, CD Search, SOPMA, PSIPRED, HHpred, etc. were employed to discover the functions and structures of Q6S8D9_SARS. After delineating the secondary and tertiary structures of the protein, some quality evaluating tools e.g., PROCHECK, ProSA-web etc. were performed to assess the structures and later the active site was identified also by CASTp v.3.0. The protein contains more negatively charged residues than positively charged residues and a high aliphatic index value which make the protein more stable. The 2D and 3D structures modeled by several bioinformatics tools ensured that the proteins had domain in it which indicated it was functional protein having the ability to trouble host antiviral inflammatory cytokine and interferon production pathways. Moreover, active site was found in the protein where ligand could bind. The study was aimed to unveil the features and structures of an uncharacterized protein of SARS-CoV which can be a therapeutic target for development of vaccines against the virus. Further research are needed to accomplish the task.

• Applied Biological Chemistry
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• v.51 no.4
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• pp.276-280
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• 2008

To elucidate the effects of ultraviolet (UV) irradiation on molecular properties of ovalbumin, the molecular weight profile, secondary structure and tertiary structure of proteins were examined after irradiation by UV with 254 nm wavelength for 4, 8, 16 and 32 hrs, respectively. UV irradiation of protein solution caused the disruption on the native state of protein molecules. SDS-PAGE and gel permeation chromatography indicated that radiation caused initial fragmentation of polypeptide chains and as a result subsequent aggregation due to cross-linking of protein molecules. Circular dichroism (CD) study showed that UV irradiation caused the change on the secondary structure resulting in decrease of helical structure or compact denature on structure of protein depending on irradiation period. Fluorescence spectroscopy indicated that irradiation quenched the emission intensity excited at 280 nm. These results suggest that UV irradiation affect the molecular properties of ovalbumin and may have potential as a means to change the antigenicity of protein allergen.

• The Journal of the Korea Contents Association
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• v.13 no.7
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• pp.24-37
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• 2013

This study aims to analyze comparatively the structure of conflict between parents and children in terms of their character with Pixar's and from the aspects of MBTI's type dynamics. Thus, the paper infers the hierarchy of the characters' personality type and psychological function and also analyzes the structure of conflict and reconciliation based on type dynamics. The study has drawn conclusions with the work analysis as follows: (1) The structure shows that the parents' thinking function restricts the children's intuitive function, and it is metaphorized as 'collision between reality and possibility', (2) As the extroverted psychological function of the character having a complementary role complements the underdeveloped tertiary function of the character with deficiency and contributes to the type development, (3) In , the subject of conflict realizes direct, unidirectional complementation, (4) And in , not because of the subject of conflict but because of the mirror character, indirect, bidirectional complementary methodology differs.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.833-840
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• 1997

Differentiation and degeneration of spikelets in paddy rice has been studied in high yielding Indica$\times$Japonica hybrid cultivar, Milyang 23 and a Japonica type cultivar, Koshihikari. Germinated seeds planted in 5000$^{-1}$ a pots filled with submerged soil and cultured under natural conditions. The young panicles of main stem were continuously dissected and observered by Cryo-SEM from the panicle initiation stage, and investigated about formation position of the differentiation and degeneration spikelet within a panicle of 7 days after heading. The degeneration of spikelet appeared simultaneously throughout panicle just after closure of spikelet by the palea and lemma. Differentiated and degenerated spikelets per panicle were about 240, 80 for Milyang 23 and 87, 6 for Koshihikari, respectively. The spikelets degeneration in Milyang 23 was mainly on the secondary and tertiary branch which were developed from primary branch of middle-basal panicle node and hardly not the spikelets of primary branch, and degeneration rate of secondary and tertiary rachis branch and spikelets for Milyang 23 were 2.5 times greater than those of Koshihikari. The proper relation equation between total differentiation or normal spikelets number per panicle(Y) and each rachis branch number were different between cultivars, Le., Y=5.5X$_1$＋3.0X$_2$ for Koshihikari as previously proposed, but those of Milyang 23, Y=5.7X$_1$＋3.5X$_2$＋2.8X$_3$ for total differentiation spikelets and Y=5.6X$_1$＋3.2X$_2$＋2.4X$_3$ for normally developed spikelets, where X$_1$, X$_2$, X$_3$ are number of primary, secondary, tertiary rachis branch, respectively.

• Bulletin of the Korean Chemical Society
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• v.29 no.8
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• pp.1510-1518
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• 2008

Results of intrinsic and extrinsic fluorescence studies on choline oxidase revealed that the enzyme at high alkaline pH values has more accessible hydrophobic patches relative to acidic pH. Fluorescence quenching studies with acrylamide confirm these changes. The quenching constants were also determined at different pH(s) by using the Stern-Volmer equation. CD studies showed that at higher pH a transition from $\alpha$-helix to $\beta$- structure was appeared while at lower pH the content of $\alpha$-helix structure was increased. Furthermore, analysis of the spectral data using chemometric method gave evidence for existence of intermediate components at very high pH(s). Results of thermal denaturation evaluated that the enzyme has the most instability at higher pH(s). Altogether low and high pH values caused significant alteration on secondary and tertiary structures of choline oxidase via inducing of an intermediate.

• Journal of Distribution Science
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• v.18 no.2
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• pp.59-67
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• 2020

Purpose: This study examines the relationship between formation of employment subcenters and regional industrial structures in Incheon. Research design, data, and methodology: We used the five-year panel data from 2012 to 2016 in 146 basic municipal units of Incheon to analyze panel regression models. Gross employment density and employment to population ratio were used as indicators of employment subcenters formation. The entropy index and Hachman index were used for analyzing the diversity and heterogeneity of industrial structures. Result: The analyses of two panel regression models showed that for the formation of employment subcenters, both the Entropy and Hachman index were significantly negative in most models. But tertiary industry was shown to have a significant positive relationship in all models. In the wholesale and retail sector, it was found that the average number of employees in the employment subcenters is significantly higher than that in the non-employment subcenters. Conclusions: The specialization of the industrial structure rather than the diversification contributes to the formation of the employment subcenters in Incheon. In particular, it can be considered that the wholesale and retail sector plays a very important role in forming the employment subcenters in many areas of Incheon.

• Korea Journal of Hospital Management
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• v.5 no.1
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• pp.200-231
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• 2000

The price systems of the hospital drug services play key roles in the provision of quality services and the development of pharmacy service technologies. Under the premises, this study attempted to determine the costs of hospital drug service, to compare the costs calculated with the fees publicly fixed by the Government, and based on the results of the analysis, to propose directions for the improvement of the price systems. A Costing model for the study was developed based on the cost-fee relationship analysed of the Korean fee-for-service systems. Data on costs and workloads of the 25 hospitals were collected through survey forms designed for the costing' and analysis for the duration of 12 months of 1998. The results of the analysis show that a tremendous unbalance between cost and price levels of the drug services, and that overally the price level of the services is extremely low when compared to the costs of services. Based on these findings, this study suggests that unfairly high or low price level be corrected, and that service items newly developed and being practiced at tertiary hospitals, such as TDM and TPN consultation services, be compensated by fixing a proper level of price.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.25 no.1
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• pp.68-77
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• 2018

Purpose: The aim of this study was to establish a hypothetical model on silence regarding patient safety and to verify the model's goodness of fit and hypotheses. Methods: The participants in this study were 330 registered nurses working in tertiary hospitals with over 300 beds. Data were collected between July 1, and August 30, 2017, from nurses who agreed to participate. A covariance structure analysis was performed. Results: The model of fit index was $x^2=59.54$, normed $x^2=2.29$, GFI=.97, AGFI=.93, SRMR=.05, NFI=.99, CFI=.95 and RMSEA=.05. The organizational culture had an influence on patient safety motivation (${\beta}=.26$, p=.003) and attitude (${\beta}=.43$, p<.001). RN-MD collaboration had an influence on patient safety motivation (${\beta}=.33$, p<.001), attitude (${\beta}=.35$, p<.001), and patient safety silence (${\beta}=-.17$, p=.026). Supervisory trust had an influence on patient safety motivation (${\beta}=.26$, p<.001), attitude (${\beta}=.12$, p=.036), and patient safety silence (${\beta}=-.23$, p=.002). Patient safety motivation had an influence on patient safety silence (${\beta}=-.33$, p=.006). The model of patient safety silence explained 36.0% of the variances. Conclusion: This study is meaningful in that it provides basic data for nursing education and program development for rejecting patient safety silence.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.6
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• pp.763-768
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• 2006

Genetic and pharmacological studies in mice have demonstrated a complementary role for the melanocortin 4 receptor (MC4R) in the control of food intake, energy balance and body weight. This study was designed to investigate the associations of a MC4R gene polymorphism on chicken growth and body composition traits in broiler lines divergently selected for abdominal fat. A SNP (G54C) was found in CDS region of chicken MC4R gene. The analysis of the least squares and variance revealed a significant association between the G54C SNP and BW, CW and SL at 7 wk of age, and there were significant differences in different genotypes (p<0.05). The results from protein secondary structure prediction and tertiary structure prediction showed that it appeared a helix in $13^{th}$ amino acid and two strands at $14^{th}$ and $15^{th}$ amino acid in mutant protein, respectively. It maybe induce the change of the activity or function of MC4R gene in poultry.

• The Transactions of the Korea Information Processing Society
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• v.2 no.5
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• pp.787-796
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• 1995

In this paper, a mechanism to enhance the utilization of the tertiary storage devices such as optical disk, digital tape and so on is proposed. For this purpose, we suggest a storage structure with a CD-ROM and multiple disk and design an efficient mechanism to represent the continuous media stored in CD-ROM. The continuous media storage structure proposed in this pater can enhanced the retrieval capability of information by sending data using a shared bus with high bandwidth. And a continuous media representation mechanism not only can reduce the discontinuity of representation using parallel operation of fetch and representation but also minimize the variance of service time and the missing ratio of re-reference requirement by residing frequently accessed object on the disk.