• Fisheries and Aquatic Sciences
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• v.26 no.3
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• pp.204-215
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• 2023

The thermolabile haemolysin (tlh) of Vibrio parahaemolyticus (Vptlh) from V. parahaemolyticus is a multiple-function enzyme, initially describes as a haemolytic factor activated by lecithin and phospholipase A2 enzymatic activity (Shinoda, 1991; Vazquez-Morado, 2021; Yanagase et al., 1970). Until now, the tlh structure has hypothesized including N-terminal and C-terminal domain, but what domain of the Vptlh structure does the haemolytic activity has not been refined yet. In this study, a 450-bp VpTLH nucleotide sequence of the entire Vptlh gene encoded the C-terminal domain cloned firstly to examine its responsibility in the activity of the Vptlh. The C-terminal domain fused with a 6-His-tag named the His-tag-VpC-terminal domain was expressed successfully in soluble form in the BL21 (DE3) PlysS cell. Remarkably, both expression and purification results confirmed a high agreement in the molecular weight of the His-tag-VpC-terminal domain was 47 kDa. This work showed the His-tag-VpC-terminal domain lysed the erythrocyte membranes in the blood agar and the phosphate buffered saline (0.9%) media without adding the lecithin substrate of the phospholipase enzyme. Haemolysis occurred at all tested diluted concentrations of His-tag-VpC-terminal domain (p < 0.05), providing evidence for the independent haemolytic activity of the His-tag-VpC-terminal domain. The content of 100 ㎍ of the His-tag-VpC-terminal domain brought the highest haemolytic activity of 80% compared to that in the three remaining contents. Significantly, the His-tag-VpC-terminal domain demonstrated not to involve the phospholipase activity in Luria-Bertani agar supplemented with 1% (vol/vol) egg yolk emulsion. All results proved the vital responsibility of the His-tag-VpC-terminal domain in causing the haemolytic activity without the required activation by the phospholipase enzyme. Raw extracts of Phellinus igniarus and Phellinus pipi at 10^-1 mg/mL inhibited the haemolytic activity of the His-tag-VpC-terminal domain from 67.7% to 87.42%, respectively. Hence applying the His-tag-VpC-terminal domain as a simple biological material to evaluate quickly potential derivatives against the Vptlh in vivo conditions will accessible and more advantageous than using the whole of the Vptlh.

• Journal of Navigation and Port Research
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• v.27 no.3
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• pp.297-306
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• 2003

As the competition in the liner shipping industry is becoming fiercer, the shipping company is trying to find the way to keep the best competitive position by reducing the cost level as low as possible and offering the best quality of service to their customer. In order to achieve this goal, the selection of port and terminal that have the best quality of service and cost effective structure is essential for the shipping line, where they can only have a chance to save the money. In this context, the paper compares three European container terminals in order to understand the factors that have to be considered in the decision making process for the selection of terminal. And its advantages and disadvantages of each type of container terminal operation system were analyzed from the liner shipping company's point of view in terms of cost, time, flexibility, stability, hinterland connection, geographical position and terminal productivity. It is obvious that the cost factors are very important for liner shipping company to select their terminal. However, there might be even more important factors than the cost factors, such as quality of port and terminal service, mutual trust, and possibility of future development.

• Journal of Korea Society of Digital Industry and Information Management
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• v.16 no.3
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• pp.1-11
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• 2020

In this paper, we describe about the user terminal tagging-based embarkation/disembarkation management system and embarkation/disembarkation management method using this system. The system authenticates the validity of the user and on whether to board on the ship by tagging the user's terminal which the boarding reservation was made by using the management terminal provided in the ship. The system identifies on whether the user disembark in the ship by tagging the user's terminal. In the event of ship accident, it is easy to figure out the user and manage the non-contact boarding and disembarking. Therefore, we design the embarkation/disembarkation management system based on user's terminal tagging on the terminal provided in the ship and embarkation/disembarkation management method using this system. User terminal tagging can solve the problem of manpower required for the management of embarkation and disembarkation, the problem of requiring time to confirm the match between the reservation and the passenger, and the problem of increase of the possibility on the spread of infectious diseases due to face-to-face contact.

• 한국정보디스플레이학회:학술대회논문집
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• 2005.07a
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• pp.464-467
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• 2005

In this paper, we present techniques to manufacture color electronic ink for multi-color electrophoretic display implementation. The charged color pigments have been prepared to have superior affinity for dielectric fluid. White $TiO_2$ nanoparticles were modified with poly(methyl methacrylate) copolymer for a microencapsulated electrophoretic display system, in order to reduce the density mismatch between nanoparticles and dielectric medium. These color balls and white pigment particle suspensions were microencapsulated through the typical microencapsulation technique. We fabricate the microcapsules to the single layer on flexible ITO substrate to test the multi-color electrophoretic display application.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2004.04a
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• pp.479-485
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• 2004

The interest of ACT (Automated Container Terminal) is increasing because of expansion of container's transportation quantity, appearance of large-sized and. high-speed vessel, high labor cost of container terminal and the change of technical level. Therefore, the ACT had been developed in several ports in the world, such as ECT (Europe Combined Terminals) and CTA (Container Terminal Altenwerder). They have studied detailed technique for the operation of ACT. In Korea, it also has increased not only expansion and improvement of container terminal, but also necessity of ACT. Now, many projects related to the development of ACT are working in Korea. And the technical improvement of operating system, automated equipments and information system has accomplished by the projects. The ACT consists of the basic equipment and the integrated information system for operating and controlling automated equipments. The productivity of ACT is maximized through efficient connection between them. Thus, the automated degree of container terminal is dependent on the developing time of operating system, reliability, transportation quantity of container, investment amount and technical level. In this paper, we propose important strategy in developing ACT through analyzing the property of each ACT.

• Journal of Korean Clinical Health Science
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• v.3 no.2
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• pp.361-371
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• 2015

Purpose. The purposed of this study was done to DNR attitude and terminal care performance among hospital nurses. Methods. The participants were 207 nurses working in hospital nurses in D and G cities. Data on DNR attitude and terminal care performance were collected via questionnaire between April 2015 and July 2015. Data analysis was done with SPSS 12.0 program and included one-way ANOVA, independent t-test, and Pearson correlation. Result. DNR attitude of participants in this study were shown to have high levels (DNR attitude: M=32.64/60, SD=6.14). Terminal care performance of participants in this study were shown to have poor levels (physical M=20.72/32, SD=3.77, psychological M=20.26/32, SD=3.85, spiritual M=9.62/24, SD=3.65). The attitude of the hospital nurse was significantly different according to the marital status. The terminal care performance was significantly different according to experience of terminal care. The DNR attitude by nurses was positively correlation to physioloical terminal care performance(r=.137, p<.049) but the relationship between the psychological terminal care performance( r=.016, p=.815) and spiritual terminal care performance showed no correlation(r=-.099, p=.157). Conclusion. The results of this study indicate that it is necessary to increase DNR attitude and to encourage terminal care performance among hospital nurses.

• Journal of Navigation and Port Research
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• v.27 no.5
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• pp.577-584
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• 2003

Container terminals are various in terms of layout, cargo handling systems and operational aspects resulting in difference in productivity and economic feasibility. Therefore, by evaluating different terminal types, more comprehensive understanding can be grasped. For this the paper aims at evaluating the three terminal types under operation, the conventional terminal, the unmaned terminal and the indented terminal, with respect to productivity and cost. Based on the results in connection with the situation of Korean container terminal operation, feasibility for each type is discussed with suggestion for the optimal type.

• The Journal of Bigdata
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• v.4 no.1
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• pp.11-27
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• 2019

There is gradually being a decrease in earnings rate of the container terminals because of worsened business environment. To enhance global competitiveness of terminal, operators of the container terminal have been attempting to deal with problems of operations through analyzing overall the terminal operations. For improving operations of the container terminal, the operators try to efforts about analyzing and utilizing data from the database which collects and stores data generated during terminal operation in real time. In this paper, we have analyzed the characteristics of operating processes and defined the event log data to generate container processes and CKO processes using stored data in TOS (terminal operating system). And we have explained how imperfect event logs creating non-normal processes are refined effectively by analyzing the container and CKO processes. We also have proposed the framework to refine the event logs easily and fast. To validate the proposed framework we have implemented it using python2.7 and tested it using the data collected from real container terminal as input data. In consequence we could have verified that the non-normal processes in the terminal operations are greatly improved.

• Journal of Korea Port Economic Association
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• v.32 no.3
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• pp.47-66
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• 2016

Once it has been built, a container terminal is impossible to move to another location. It is hard to rectify wrong decisions in a container terminal. This highlights the importance of decision making for a container terminal. The port management about a container terminal has developed from a cargo interface location between sea and land transport, to the standardization of information and procedures due to globalization among global shipping and terminal operators. This research focuses on the current states of market and management for global container terminal operators by investigating up-to-date data for them. The current market states for global container terminal operators are analyzed by using by Herfindahl-Hirschman Index. The analyses of current management states for global container terminal operators are divided into profitability analysis, activity analysis, and bankruptcy risk analysis. Finally, global container terminal operators are clustered into three groups by the current management states.

• International Journal of Control, Automation, and Systems
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• v.2 no.1
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• pp.15-22
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• 2004

Some recent advances in stability and optimality for the nonlinear receding horizon control (NRHC) or the nonlinear model predictive control (NMPC) are assessed. The NRHCs with terminal conditions are surveyed in terms of a terminal state equality constraint, a terminal cost, and a terminal constraint set. Other NRHCs without terminal conditions are surveyed in terms of a control Lyapunov function (CLF) and cost monotonicity. Additional approaches such as output feedback, fuzzy, and neural network are introduced. This paper excludes the results for linear receding horizon controls and concentrates only on the analytical results of NRHCs, not including applications of NRHCs. Stability and optimality are focused on rather than robustness.