• Korean Chemical Engineering Research
• /
• 제46권2호
• /
• pp.227-232
• /
• 2008

화장품용 천연계면활성제를 개발하기 위해 도라지(platycodon grandiflorum) 껍질에서 유기용매를 이용하여 추출물을 얻었다. 도라지 추출물의 화장품용 계면활성을 측정하기 위해 계면장력, 용해도, 기포력, 분산력, 유화력, 유화활성, 유화안정성을 측정하였고 피부자극을 평가하기위해 첩포시험을 실시하였다. 도라지 사포닌 함량이 0.005 wt%일 때 castor oil에 대한 계면장력은 11.5 dyn/cm로 비교대상인 Tween 40이나 quillaja bark보다 낮은 계면활성을 보여주었다. 도라지 추출물은 화장품에 유상으로 사용되는 olive oil, soybean oil, canola oil에 대해서도 우수한 유화활성과 유화안정성을 나타내었다. 2~5%의 도라지 추출물과 글리세린을 사용한 피부자극 시험에서는 도라지 추출물은 약간의 피부자극을 보여 주었다. 결론적으로 도라지 추출물은 화장품용 계면활성력은 양호하나 다소의 피부자극을 보여주었다.

• Journal of Chest Surgery
• /
• 제22권1호
• /
• pp.10-15
• /
• 1989

Excision of bullous emphysema or decortication of chronic empyema commonly results in a prolonged air leakage. Prolonged air leakage requires prolonged intercostal drainage, delays recovery, and can be followed complications such as pneumothorax, atelectasis, incomplete expansion of remained lung, secondary infection. To minimize these complications free muscle grafts can be used like a patch to close the opening of visceral pleura and reinforce suture lines without undue tension. From a preliminary study using the latissimus dorsi muscle as a free muscle graft in the rabbit pleural space, viable muscle fibers that seems the result of the process of regeneration can be consistently identified around the degenerating muscle fibers. Voluminous connective tissues and numerous blood vessels are also observed in the peripheral zone. Further studies in that free muscle graft will be sutured with visceral pleura and lung parenchyme will hopefully provide additional information before clinical application.

• 대한기계학회논문집
• /
• 제18권12호
• /
• pp.3395-3403
• /
• 1994

The structural test technique and equipment for strength test of astronautical structures, such as rocket, were presented in this paper. Structural strength tests of the fin assembly with fin and fin frame in the scientific sound rocket were performed with load levels of 100% limit load and 150% ultimate load of design lift force. Safety factors in each part of the fin assembly were calculated at these two load levels and the stiffnesses based on the measured deflection of fin assembly and strains on fin and fin frame were evaluated at these two load level. As the result of structural test, the fin assembly was estimated to be safe.

• Experimental and Molecular Medicine
• /
• 제50권12호
• /
• pp.11.1-11.9
• /
• 2018

Estrogen has diverse effects on cardiovascular function, including regulation of the contractile response to vasoactive substances such as serotonin. The serotonin system recently emerged as an important player in the regulation of vascular tone in humans. However, hyperreactivity to serotonin appears to be a critical factor for the pathophysiology of hypertension. In this study, we examined the modulatory mechanisms of estrogen in serotonin-induced vasoconstriction by using a combinatory approach of isometric tension measurements, molecular biology, and patch-clamp techniques. $17{\beta}$-Estradiol (E2) elicited a significant and concentration-dependent relaxation of serotonin-induced contraction in deendothelialized aortic strips isolated from male rats. E2 triggered a relaxation of serotonin-induced contraction even in the presence of tamoxifen, an estrogen receptor antagonist, suggesting that E2-induced changes are not mediated by estrogen receptor. Patch-clamp studies in rat arterial myocytes showed that E2 prevented Kv channel inhibition induced by serotonin. Serotonin increased Src activation in arterial smooth muscle required for contraction, which was significantly inhibited by E2. The estrogen receptor-independent inhibition of Src by E2 was confirmed in HEK293T cells that do not express estrogen receptor. Taken together, these results suggest that estrogen exerts vasodilatory effects on serotonin-precontracted arteries via Src, implying a critical role for estrogen in the prevention of vascular hyperreactivity to serotonin.

• 한국산학기술학회논문지
• /
• 제19권12호
• /
• pp.14-24
• /
• 2018

몇 가지 전형적인 기존 및 진보된 초탄성 구성모델들의 고무패치 이축인장 및 구형 또는 원통형 풍선 팽창에서의 불안정성에 대해서 밝힌다. 적용할 구성모델은 neo-Hookean 모델, Mooney-Rivlin 모델, Gent 모델, Arruda-Boyce 모델, Fung 모델, Pucci-Saccomandi 모델 등이다. 팽창 및 분기 해석은 이들 변형에너지 함수들의 막 방정식을 이용하여 수행할 수 있다. 해석에는 사각패치에 대한 Kearsley의 분기현상, 고무풍선의 일반화 한 팽창현상, 고무풍선의 분기현상을 다룬다. 이들 변형에너지 함수들 중에서도 오직 Mooney-Rivlin 모델에서만 Kearsley의 분기현상이 일어남을 확인하였다. 팽창 방정식은 구형풍선과 원통형 풍선을 함께 다룰 수 있도록 일반화 시켰다. 팽창해석에 의하여 극한점과 임계 물성치들을 무차원 압력 및 팽창 부피의 항들로 구하였다. 그렇게 구해진 결과들로부터 분기현상을 구할 수 있었다. 또한 유한요소법을 사용하여 고무류의 구조적 불안정 문제들을 다룰 때 필요한 특별한 조처에 대해서 제안하였다. 결론적으로 고무류의 불안정성을 포함하는 문제를 다룰 때는 해석기법은 물론 구성모델의 선택에 따라 결과가 달라질 수 있으므로 신중한 처리가 요구된다.

• The Korean Journal of Physiology and Pharmacology
• /
• 제18권6호
• /
• pp.503-508
• /
• 2014

Spontaneous myometrial contraction (SMC) in pregnant uterus is greatly related with gestational age and growing in frequency and amplitude toward the end of gestation to initiate labor. But, an accurate mechanism has not been elucidated. In human and rat uterus, all TRPCs except TRPC2 are expressed in pregnant myometrium and among them, TRPC4 are predominant throughout gestation, suggesting a possible role in regulation of SMC. Therefore, we investigated whether the TRP channel may be involved SMC evoked by mechanical stretch in pregnant myometrial strips of rat using isometric tension measurement and patch-clamp technique. In the present results, hypoosmotic cell swelling activated a potent outward rectifying current in G protein-dependent manner in rat pregnant myocyte. The current was significantly potentiated by $1{\mu}M$ lanthanides (a potent TRPC4/5 stimulator) and suppressed by $10{\mu}M$ 2-APB (TRPC4-7 inhibitor). In addition, in isometric tension experiment, SMC which was evoked by passive stretch was greatly potentiated by lanthanide ($1{\mu}M$) and suppressed by 2-APB ($10{\mu}M$), suggesting a possible involvement of TRPC4/5 channel in regulation of SMC in pregnant myometrium. These results provide a possible cellular mechanism for regulation of SMC during pregnancy and provide basic information for developing a new agent for treatment of premature labor.

• The Korean Journal of Physiology and Pharmacology
• /
• 제4권6호
• /
• pp.479-486
• /
• 2000

The aim of this study was to clarify the mechanism of the inhibitory action of carbon monoxide (CO) on contraction, by measuring cytosolic $Ca^{2+}$ level $([Ca^{2+}]_i)$ and ionic currents in guinea-pig ileum. CO (10%) inhibited 40 mM KCl-induced contraction and this effect was blocked by ODQ $(1\;{\mu}M),$ a soluble guanylyl cyclase (sGC) inhibitor. CO inhibited the 40 mM KCl-induced contraction without changing $[Ca^{2+}]_i.$ Cumulative addition of KCl induced a graded increase in $[Ca^{2+}]_i$ and muscle tension. In the presence of CO, cumulative addition of KCl induced smaller contraction than in the absence of CO. On the other hand, the increase in $[Ca^{2+}]_i$ induced by cumulative addition of KCl was only slightly decreased in the presence of CO, and the $[Ca^{2+}]_i-tension$ relationship shifted downwards. Using the patch clamp technique with a holding potential of -60 mV, we found that CO had little effect on the peak Ba currents $(I_{Ba})$ when voltage was stepped from -60 mV to 0 mV. In addition, CO showed no effect on the depolarization-activated outward $K^+$ currents in the all potential ranges. We conclude that CO inhibits smooth muscle contraction mainly by decreasing the $Ca^{2+}$ sensitivity of contractile elements via a cGMP-dependent pathway, not by involving L-type $Ca^{2+}$ and outward-potassium currents in guinea-pig ileum.

• Applied Biological Chemistry
• /
• 제48권4호
• /
• pp.404-410
• /
• 2005

Sopoongsan is an oriental medicinal composition including 12 medicinal herbs. Sopoongsan is known to have anti-inflammatory, antimicrobial, anti-allergic, and anticancer effects on human skin. The results of stability test showed that the creams containing Sopoongsan extracts were very stable at both accelerated temperature conditions and sun-light. And pH and viscosity of each cream did not change greatly for 56 days. From the particle size and rheological measurements, it can be known that when the amount of the Sopoongsan extracts increases, the mean value of particle size decreases, and the value of the complex modulus and loss angle increases, which means the Sopoongsan extracts help stabilize the emulsion. From the result of human patch test to assess the safety of cream containing Sopoongsan extracts, there was no stimulus of negative reaction on skin. In result of the user tests, it can be known that the higher concentration of the Sopoongsan was preferred by customers.

• The Korean Journal of Physiology and Pharmacology
• /
• 제4권4호
• /
• pp.333-338
• /
• 2000

We have examined whether bile acids can affect the electrical and mechanical activities of circular smooth muscle of canine colon and ileum, using isometric tension measurement or patch clamp technique. It was found that a dilution of canine bile $(0.03{\sim}2%\;by\;volume)$ enhanced or inhibited the amplitude of spontaneous contractions. An individual component of bile, deoxycholic acid (DCA) enhanced the frequency and amplitude of the spontaneous contractile activity at $10^{-6}\;M,$ while DCA at $10^{-4}\;M$ inhibited the contraction. Similarly, the response to cholic acid was excitatory at $10^{-5}\;M$ and inhibitory at $3{\times}10^{-4}\;M.$ Taurocholic acid at $10^{-4}\;M$ enhanced the amplitude of muscle contraction. Electrically, canine bile at 1% reversibly depolarized the colonic myocytes under current clamp mode. Bile acids also elicited non-selective cation currents under voltage clamp studies, where $K^+$ currents were blocked and the $Cl^-$ gradient was adjusted so that $E_{Cl}^-$ was equal to -70 mV, a holding potential. The non-selective cation current might explain the depolarization caused by bile acids in intact muscles. Furthermore, the bile acid regulation of electrical and mechanical activities of intestinal smooth muscle may explain some of the pathophysiological conditions accompanying defects in bile reabsorption.

• 한국의류산업학회지
• /
• 제4권4호
• /
• pp.327-338
• /
• 2002

The analysis and examination of this study are focussed on the external deconstruction trend expressed in the works of Jean Paul Gaultier. The external deconstruction is a way of expression faithful to the literal meaning of 'deconstruction' and is the applied case of exposure, destruction, poverty, and decomposition as they are. The method and scope of this study are from 1980's to present, and the followings are the results of this examination focussed on the various literature of philosophy, aesthetics and literary criticism, and the domestic and foreign fashion journals. The exposure phenomena through the deconstruction expressed repeatedly in the works of Gaultier deconstructed the fixed idea of 'the inner wear should be worn inside the outer wear' and at the same time denied the dichotomical interpretation of the exposure and suppression, the traditional beauty and decadent beauty, the chastity and unchastity, the asceticism and sexuality, and obscured the notion of the inner wear and outer wear. The destructive deconstruction expressed in the works of Jean Paul Gaultier introduced the elements such as hippy, punk, and kitsch, slashed before making dresses, crumpled unseemly like wastepaper, or made dresses with textures like paper scraps, and through destroying textures, yielded shock effects and tension. Poverty, through borrowing from the outwardly poor-looking elements of design, i.e. the patch work, decolor, dye, fading, fringing, incompletion, and handmadeness, liberated dresses and their ornaments from the outside. The traditional dresses were dresses having certain forms with formative beauty, but Gaultier disassembled dresses and raised questions about the logic of dresses themselves.