• Animal cells and systems
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• 제14권2호
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• pp.73-81
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• 2010

In this study, monoclonal antibodies against lysosomal acid phosphatase (LAP) of a salamander, Hynobius leechii, were used to determine the spatial and temporal expression of the LAP in the regenerating limbs. The Western blot and immunohistochemical analysis in the limb regeneration revealed that LAP was highly expressed at the dedifferentiation stage, especially in the wound epidermis and dedifferentiating limb tissues such as muscle and cartilage. With RA treatment, the LAP expression became upregulated in terms of both level and duration in the wound epidermis, blastemal cell and dedifferentiating limb tissues. In addition, in situ activity staining of LAP showed a similar result to that of immunohistochemistry. Thus, the activity profile of LAP activity coincides well with the expression profile of LAP during the dedifferentiation period. Furthermore, to examine the effects of lysosomal enzymes including LAP on salamander limb regeneration, lysosome extract was microinjected into limb regenerates. Interestingly, when the lysosome extract was microinjected into limb regenerates with a low dose of RA($50\;{\mu}g/g$ body wt.), skeletal pattern duplication occurred frequently in the proximodistal and transverse axes. Therefore, lysosomal enzymes might cause the regenerative environment and RA plays dual roles in the modification of positional value as well as evocation of extensive dedifferentiation for pattern duplication. In conclusion, these results support the hypothesis that dedifferentiation is a crucial event in the process of limb regeneration and RA-evoked pattern duplication, and lysosomal enzymes may play important role(s) in this process.

• Journal of Oral Medicine and Pain
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• 제22권1호
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• pp.145-155
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• 1997

The purpose of this study was to compare differences in endurance time and EMG power spectral characteristics of the masticatory muscles during sustained isometric contraction between patients and controls. 15 CMD patients{8 women and 7 men, aged 15 to 38 years(24.1$\pm$7.5)}, and 15 healthy volunteers{8 women and 7 men, aged 15 to 30 years(24.7$\pm$3.4)} without past history or present symptoms of CMD were included in this study. Sustained isometric contractions of masticatory muscles were perfomeed as long as possible at 50% level of maximum voluntary contraction(MVC) of EMG activity via visual feedback, and the duration of sustained isometric contraction(endurance time) was examined. The author perfomed EMG power spectral analysis in the myoelectric signals of masseter and anterior temporal muscle during sustained isometric contraction in CMD patients with chronic muscle pain and healthy controls. The author came to following conclusions from the results. 1. The endurance time of the patient group was shorter than the control group in sustained isometric contraction of masticatory muscles(p<0.01). 2. MF values of masticatory muscles with sustained isometric contraction during endurance time were decreased following regression line in both groups(p<0.01, r>0.9). 3. The amount of MF shift to lower frequency range exhibited no significant differences between the patients and the control group in sustained isometric contraction during endurance time. 4. SMF to lower frequency range of the patient group was steeper than the control group in sustained isometric contraction during endurance time(p<0.05).

• 말소리와 음성과학
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• 제12권2호
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• pp.17-27
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• 2020

This paper examines kinematic characteristics of /pk/ clusters, as compared to /kk/ and /pp/ with varying vowel contexts and speech rate. The results of EMMA data from eight Seoul-Korean speakers indicate as follows. Firstly, comparing /pk/ to /pp/ sequences, lips closing movement was faster and spatially greater in the /a/-to-/a/ context while temporally longer in the /i/-to-/i/ context. It was smaller in spatial displacement and shorter in temporal duration in /pk/ sequences. Peak velocity did not vary. Secondly, comparing /pk/ with /pp/ and /kk/ controls, lip aperture was less constricted in the /a/-to-/a/ context than /i/-to-/i/, but the maximum contact between the upper and lower lips was invariant across different vocalic contexts within /pk/ sequences (/apka/=/ipki/). Categorical reduction of C1 in /pk/ sequences fell in with the low-vowel and fast-rate conditions with across-/within-speaker variability. Gradient reduction of C1 was observed in all C1C2 types, being more frequent in fast rate. Lastly, the jaw articulator was a stable indicator of rate effects. The implication of the current study is that gestural reduction occurs with categorical reduction and general spatiotemporal weakening in the assimilating contexts, while quantitative properties of gestures may be a reason for gradient reduction, not necessarily confined to place assimilation.

• 대한산업공학회지
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• 제29권4호
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• pp.271-282
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• 2003

Automotive occupant packaging has been a part of main ergonomics interests, especially, in terms of driver's posture. Previous research on driver's posture has mainly focused on the initial optimal posture for driving sedans. However, customer preferences on cars are shifting from sedans to RV and automobile manufacturing companies seek to understand temporal changes in drivers' posture according to driving environment. So the main aim of this study was to develop a driver's posture measurement system during driving and investigate casual changes due to duration, car type, traffic flow. Four male drivers participated in the experiments during one week. It was shown that considerable changes in their postures were caused with respect to driving environment, which implies that not only static optimal postures but their dynamic changes should be taken into consideration for proper design and evaluation of interior packaging. The research is expected to help packaging designers understand human drivers so as to improve their comfort.

• 자원환경지질
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• 제28권5호
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• pp.527-533
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• 1995

A disadvantage of Fourier analysis is that frequency information can only be extracted for the complete duration of a signal f(t). Since the Fourier transform integral extends over all time, from $-{\infty}$ to $+{\infty}$), the information it provides arises from an average over the whole length of the signal. If there is a local oscillation representing a particular feature, this will contribute to the calculated Fourier transform $F({\omega})$, but its location on the time axis will be lost There is no way of knowing whether the value of $F({\omega})$ at a particular ${\omega}$ derives from frequencies present throughout the life of f(t) or during just one or a few selected periods. This disadvantage is overcome in wavelet analysis which provides an alternative way of breaking a signal down into its constituent parts. The main advantage of the wavelet transform over the conventional Fourier transform is that it can not only provide the combined temporal and spectral features of the signal, but can also localize the target information in the time-frequency domain simultaneously. The wavelet transform distinguishes itself from Short Time Fourier Transform for time-frequency analysis in that it has a zoom-in and zoom-out capability.

• 대한음성학회지:말소리
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• 제61호
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• pp.63-74
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• 2007

Discrimination of music and speech from the multimedia signal is an important task in audio coding and broadcast monitoring systems. This paper deals with the problem of feature parameter extraction for discrimination of music and speech. The wavelet transform is a multi-resolution analysis method that is useful for analysis of temporal and spectral properties of non-stationary signals such as speech and audio signals. We propose new feature parameters extracted from the wavelet transformed signal for discrimination of music and speech. First, wavelet coefficients are obtained on the frame-by-frame basis. The analysis frame size is set to 20 ms. A parameter $E_{sum}$ is then defined by adding the difference of magnitude between adjacent wavelet coefficients in each scale. The maximum and minimum values of $E_{sum}$ for period of 2 seconds, which corresponds to the discrimination duration, are used as feature parameters for discrimination of music and speech. To evaluate the performance of the proposed feature parameters for music and speech discrimination, the accuracy of music and speech discrimination is measured for various types of music and speech signals. In the experiment every 2-second data is discriminated as music or speech, and about 93% of music and speech segments have been successfully detected.

• Current Research on Agriculture and Life Sciences
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• 제32권3호
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• pp.135-143
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• 2014

The variability and temporal trends of the annual and seasonal minimum and maximum temperature, rainfall, relative humidity, wind speed, sunshine hours, and runoff were analyzed for 5 major rivers in Korea from 1960 to 2010. A simple regression and non-parametric methods (Mann-Kendall test and Sen's estimator) were used in this study. The analysis results show that the minimum temperature ($T_{min}$) had a higher increasing trend than the maximum temperature ($T_{max}$), and the average temperature increased by about $0.03^{\circ}C\;yr.^{-1}$. The relative humidity and wind speed decreased by $0.02%\;yr^{-1}$ and $0.01m\;s^{-1}yr^{-1}$, respectively. With the exception of the Han River basin, the regression analysis and Mann-Kendall and Sen results failed to detect trends for the runoff and rainfall over the study period. Rapid land use changes were linked to the increase in the runoff in the Han River basin. The sensitivity of the evapotranspiration and ultimately the runoff to the meteorological variables was in the order of relative humidity > sunshine duration > wind speed > $T_{max}$ > $T_{min}$. Future studies should investigate the interaction of the variables analyzed herein, and their relative contributions to the runoff trends.

• 말소리와 음성과학
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• 제13권1호
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• pp.45-51
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• 2021

End-to-end (E2E) automatic speech recognition (ASR) has achieved promising performance gains with the introduced self-attention network, Transformer. However, due to training time and the number of hyperparameters, finding the optimal hyperparameter set is computationally expensive. This paper investigates the impact of hyperparameters in the Transformer network to answer two questions: which hyperparameter plays a critical role in the task performance and training speed. The Transformer network for training has two encoder and decoder networks combined with Connectionist Temporal Classification (CTC). We have trained the model with Wall Street Journal (WSJ) SI-284 and tested on devl93 and eval92. Seventeen hyperparameters were selected from the ESPnet training configuration, and varying ranges of values were used for experiments. The result shows that "num blocks" and "linear units" hyperparameters in the encoder and decoder networks reduce Word Error Rate (WER) significantly. However, performance gain is more prominent when they are altered in the encoder network. Training duration also linearly increased as "num blocks" and "linear units" hyperparameters' values grow. Based on the experimental results, we collected the optimal values from each hyperparameter and reduced the WER up to 2.9/1.9 from dev93 and eval93 respectively.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• 제25권2호
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• pp.54-65
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• 2021

In this study, a two-dimensional thermoelastic problem under hyperbolic heat conduction theory with an internal heat source is considered. The general solution for the temperature field, stress components and displacement field are obtained using the reduced differential transform method. The stress and displacement components are obtained using the thermal stress function in the reduced differential transform domain. All the solutions are obtained in the form of power series. The special case with a time-dependent laser heat source has been considered. The problem is considered for homogeneous material with finite rectangular cross-section heated with a non-Gaussian temporal profile. The effect of the heat source on all the characteristics of a material is discussed numerically and graphically for magnesium material taking a pulse duration of 0.2 ps. This study provides a powerful tool for finding the solution to the thermoelastic problem with less computational work as compared to other methods. The result obtained in the study may be useful for the investigation of thermal characteristics in engineering and industrial applications.

• The Journal of Korean Physical Therapy
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• 제34권6호
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• pp.326-331
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• 2022

Purpose: The stroke patients have gait dysfunction due to impaired neural tracts; corticospinal tract (CST), corticoreticular pathway (CRP), and vestibulospinal tract (VST). In this study, we investigated characteristics of gait pattern according to the injury aspect of the neural track in a stroke patient. Methods: One patient and six control subjects of similar age participated. A 19-year-old male patient with spontaneous intracerebral hemorrhage on right basal ganglia, thalamus, corona radiata and cerebral cortex due to arteriovenous malformation rupture. Diffusion tensor imaging (DTI) data was acquired 21 months after the stroke. Kinematic and spatio-temporal parameters of gait were collected using a three-dimensional gait analysis system. Results: On 21 months DTI, the CST and CRP in affected hemisphere showed severe injury, in contrast, the VST in affected hemisphere showed intact integrity. Result of gait analysis, walking distance and speed were significantly decreased in a patient. The stance rate of unaffected lower limb, the swing rate of affected lower limb and the duration of double stance significantly increased compared with normal control. The knee and hip joint angle were significantly decreased in a patient. Conclusion: We found recovered independent gait ability may be associated with unimpaired VST in a patient with severe injury in CST and CRP.