• Journal of Applied Biological Chemistry
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• v.59 no.4
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• pp.361-364
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• 2016

We developed a spectrophotometric assay method for polyethoxylated ascorbic acidusing 3-ethyl ascorbic acid as standard material. The analytical method was validated by linearity, accuracy, precision, and stability. The coefficient of variation of the precision of the assay was less than 3.4 %. The linearity of the calibration curves in the desired concentration range is good ($r^2$>0.998). 3-Ethyl ascorbic acid and polyethoxylated ascorbic acid were stable in stock solution at room temperature for up to at least 6 h. The developed assay could be used for the content analysis of polyethoxylated ascorbic acid in samples.

• Journal of the Korean Chemical Society
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• v.44 no.6
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• pp.556-562
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• 2000

The determination of chlorite ion by flow injection analysis(FIA) with iodometric UV detection were investigated. Under rather acidic condition, chlorite ion react with iodide ion to form iodine and itself is reduced to chloride ion. The chlorite ion was determined indirectly by measuring absorbance of yellow colored iodine at 370 nm. The lengths of the mixing coil and the reaction coil, the pH of the acid stream, the concentration of the iodide ion, the injection loop volume, temperature, and flowrate were optimized as parameters for selectively determining a sort of inorganic disinfection by-product, chlorite ion by using FIA-UV detection setup. Masking agents for removing oxidants or interferences from the prepared water were tested. Independent calibration curve presented linear range of 0.002-0.2 mg/L for chlorite ion with a correlation coefficient of 0.999 or better. The limit of detection(LOD) was 0.18 ${\mu}g/L$ for chlorite ion.

• Journal of the Korean Geographical Society
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• v.46 no.3
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• pp.319-330
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• 2011

In this study, a soil moisture estimation model was proposed using the ground observation data of soil moisture, precipitation, surface temperature, MODIS NDVI and artificial neural networks. The model was calibrated and verified on the Yongdam dam watershed which has reliable ground soil moisture networks. The test statistics of calibration sites, Jucheon, Bugui, Sangjeon, showed that the correlation coefficients between observations and estimations are about 0.9353 and RMSE is about 1.4957%. Also that of the verification site, Cheoncheon2, showed that the correlation coefficient is about 0.8215 and RMSE is about 4.2077%. The soil moisture estimation model was applied to estimate the spatial distribution of soil moisture in the Yongdam dam watershed and results showed improved spatial soil moisture distribution since the model used satellite information of NDVI and artificial neural networks which can represent the nonlinear relationships between data well. The model should be useful to estimate wide range soil moisture information.

• The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.11 no.1
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• pp.114-122
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• 1997

This paper describes the design and development of a smart digital load cell used forweighing installations. Sice the load cell sensor to be used is very sensitive for weight cariation, the load cell must have the temperature stability, low-drift and the high-resolution of the A/D conversion for accuracy. A new analog circuit which is controlled by one chip micro-processer has been developed to reduce the offset voltage and the drift characteristics of operational amplifiers, and has been adapted into the digital load cell. Also, a software algorithm has been developed to obtain the stable and accurate A/D conversion. This software includes a RS-485 communication program to control the digital load cell, which gives a capability of backing-up the calibration data and transferring control data. The simulation and evaluation of the designed digital load cell has been shown as having the good performance. which will give useful application to the weighing installations as a remote weighing sensor.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.8
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• pp.2555-2560
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• 1996

Inspecting the dimensional accuracy of a car-body in assembly line is a very important process to assure high productivity. Now there exist two common inspecting methods in practice. One is to measure a sampled car-body with three dimensional measuring machine, and the other is to measure car-body with three dimensional measuring machine, and the other is to measure car-body in assembly line using many sensors fixed to a large jig frame. The formal method takes too long to inspect a sampled car-body of a same sort, and cannot therefore give an useful error trend for the whole production. On the other hand, the latter lacks flexibility and is very cost-intensive. By using industrial robots and sensors, an in-line Car-Body Measuring(CBM) system which ensured high flexiblity and sufficient accuracy was developed. This CBM cell operates in real production line and measures the check points by the non-contact type using camera and laser displacement sensor(LDS). This system can handle about 15 Measuring points within a cycle time of 40 seconds. A process computer controls whole process such as data acquisition file handling and data analysis. Robot arms changes in length due to ambient temperature fluctuation affecting the measuring accuracy. To compensate this error, a robot arm calibration process was developed.

• YAKHAK HOEJI
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• v.52 no.6
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• pp.446-451
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• 2008

A high-performance liquid chromatography (HPLC) with diode array detector (DAD) and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of chlorogenic acid (1), sweroside (2), luteolin-7-O-glucoside (3), (E)-aldosecologanin (4) and 3,5-dicaffeoylquinic acid (5) from Lonicera joponica flower buds. The optimal chromatographic conditions were obtained on an ODS column (5 ${\mu}m$, 4.6${\times}$150 mm) with the column temperature $25^{\circ}C$. The mobile phase was composed of (A) water with 0.1% formic acid and (B) acetonitrile with 0.1% formic acid using a gradient elution, the flow rate was 0.3 ml/min. Detection wavelength was set at 250 nm. All calibration curves showed good linear regression ($r^2$>0.994) within test ranges. The developed method provided satisfactory precision and accuracy with overall intra-day and inter-day variations of 0.05${\sim}$1.95% and 0.15${\sim}$2.26%, respectively, and the overall recoveries of 97.71${\sim}$103.65% for the five compounds analyzed. The verified method was successfully applied to quantitative determination of the three types (phenolic compounds, iridoids and flavonoids) of bioactive compounds in 21 commercial L. japonica flower buds samples from different markets in Korea and China. The analytical results demonstrated that the contents of the five analytes vary significantly with sources.

• The Korea Journal of Herbology
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• v.23 no.3
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• pp.27-32
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• 2008

Objectives : In this paper, we describe that $^1H-NMR$ spectroscopy may be superior to the conventional HPLC for the quantitative analysis of hesperidin from Citrus unshiu. Methods : $^1H-NMR$ spectra (400 MHz) were recorded in $DMSO-d_6$ using a Varian UNITY Inova AS 400 FT NMR spectrometer. One hundred milligram of powdered Citrus unshiu was weighed out and mixed with 1 ml of $DMSO-d_6$ with sonication for 30 min (room temperature). The extracts were filtrated through a 0.45 ${\mu}m$ PVDF filter and 0.5 ml of filtrated extract used for quantitative $^1H-NMR$ measurement (added 1 mg of dimethyl terephthalate as internal standard). The quantity of hesperidin was calculated by the ratio of the intensity of the compound to the known amount of internal standard. For HPLC analysis, the half gram of plant material was extracted with 60 ml of MeOH for 2 hours. The extracts were made 100 ml volume and analyzed by a Waters HPLC system using a YMC ODS column. The total flow rate was 1.0 ml/min with a sample volume 10 ${\mu}l$ and UV detection at 280nm. Results : The contents of hesperidin in Citrus unshiu was determined $5.33{\pm}0.06$% in the quantitative $^1H-NMR$ method and $5.15{\pm}0.12%$ in HPLC method. Using the quantitative $^1H-NMR$ the contents of hesperidin can be determined in much shorter time than the conventional HPLC measurements. Conclusions : From those results, the advantages of quantitative $^1H-NMR$ analysis are that can be analyzed to identify and quantify, and no reference compounds required for calibration curve. Besides, it allows rapid and simple quantification for hesperidin with an analysis time for only 10 min without any pre-purification steps.

• Journal of Korea Water Resources Association
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• v.43 no.3
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• pp.283-294
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• 2010

The objective of this study is to develop an accurate and stable dynamic water quality model which is capable of reflecting various flows and irregular cross sections and handling numerical oscillations under the low flow conditions. In order to solve the oscillation problem under the low flow conditions, diffusive wave method was applied to the low flow condition in developing a hydraulic model, DyHYD. DyQUAL is also developed as a water quality model to calculate up to 12 water quality variables including autochthonous BOD, water temperature, DO, TN and TP. The developed model is applied to both hypothetical river channels and actual Nakdong river watershed. Additionally, the applicability and reliability of the models are verified by comparing simulation results with observed values. Nash-Sutcliffe coefficients are estimated by comparison between simulation results and observed values. In the calibration and verification process, the coefficients varies from 0.391 to 0.591 and 0.704 to 0.902 for discharge, BOD, TN and TP, respectively.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.4
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• pp.313-319
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• 2017

Fiber optics sensors that have been mainly applied to aerospace areas are now finding applicability in other areas, such as transportation, including railways. Among the sensors, the fiber Bragg grating (FBG) sensors have led to a steep increase due to their properties of absolute measurement and multiplexing capability. Generally, the FBG sensors adhere to structures and sensing modules using adhesives such as an epoxy. However, the measurement errors that occurred when the FBG sensors were used in a long-term application, where they were exposed to environmental thermal load, required calibration. For this reason, the thermal curing of adhesives needs to be investigated to enhance the reliability of the FBG sensor system. This can be done at room temperature through cyclic thermal load tests using four types of specimens. From the test results, it is confirmed that residual compressive strain occurs to the FBG sensors due to an initial cyclic thermal load. In conclusion, signals of the FBG sensors need to be stabilized for applying them to a long-term SHM.

• Journal of Pharmaceutical Investigation
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• v.21 no.2
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• pp.67-71
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• 1991

A simple and sensitive method was developed for the quantification of free and conjugated bile acids in bezoar without prior hydrolysis. $3{\alpha}-Hydroxy$ bile acids are first oxidized to 3-keto bile acids in the reaction catalyzed by $3{\alpha}-hydroxysteroid$ $dehydrogenase(3{\alpha}-HSD)$. During this oxidative reaction, an equimolar quantity of nicotinamide adenine dinucleotide(NAD) is reduced to NADH and subsequently oxidized to NAD with concomitant reduction of nitrotetrazolium blue(NTB) to diformazan by the catalytic action of diaphorase. The diformazan has an absorbance maximum at 540 nm. The intensity of the color produced is directly proportional to bile acids concentration in the bezoar extracts. The optimum conditions for the enzymatic reaction such as effects of reaction time, reaction temperature and pH, and stability were investigated. Calibration plots for the sodium chelate observed to be linear and intra-, inter-assay analytical recovery of bile acids averaged $97.65{\pm}3.4%(S.D.)$. Therefore, it is considered that the quality control of total bile acids from bezoar or bezoar-containing liquid preparation using this simple and sensitive assay system will be acceptable. Also current bezoars and bezoar-containing liauid preparations were examined their total bile acids from this method.