• Korean Journal of Fisheries and Aquatic Sciences
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• 제55권5호
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• pp.583-589
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• 2022

The feeding habits of the marbled flounder Pseudopleuronectes yokohamae were studied using 384 specimens (10.7-52.3 cm in total length) collected from the West Sea, Korea between January and December 2021. P. yokohamae ate mainly polychaeta and gastropoda, incuding teleostei, caridea, euphausiacea, amphipoda and brachyura. The mean number of preys per stomach (mN/ST) showed a statistically significant difference, and the mean weight of prey per stomach (mW/ST) increased as the total length increased. P. yokohamae ate mainly teleostei in spring and winter. P. yokohamae ate mainly euphausiacea in summer and mainly polychaeta in autumn.

• Korean Journal of Ichthyology
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• 제35권4호
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• pp.325-332
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• 2023

The feeding habits of the Fat Greenling Hexagrammos otakii were studied using 376 specimens (17.4~41.4 cm in total length) collected off the Incheon coast, Korea between March 2020 and February 2021. H. otakii ate mainly gastropoda and teleostei, incuding caridea, brachyura, polychaeta, cephalopoda and amphipoda. The diet composition of H. otakii showed changes in season. H. otakii ate mainly cephalopoda in spring and ate mainly polychaeta in summer. The teleostei feeding rate was highest in autumn, whereas the proportion of gastropoda was higher in winter than in other seasons. The mean number of preys per stomach (mN/ST, One-way ANOVA, F=2.026, P>0.05) and mean weight of preys per stomach (mW/ST, One-way ANOVA, F=1.075, P>0.05) didn't show a statistically significant difference among size classes.

• Korean Journal of Ichthyology
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• 제21권3호
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• pp.141-148
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• 2009

Sex differentiation and gonad development were investigated in a marine medaka species, Oryzias dancena (Beloniformes; Teleostei). The average time to hatch was 11 days post-fertilization (dpf) at $25^{\circ}C$. Primordial germ cell (PGC) was first observed at 5 dpf and migrated to presumptive gonadal area between the gut and pronephric duct at 9 dpf. Male and female gonads were morphologically differentiated at 12 days post-hatching (dph). Early oocytes at perinucleolus stage as well as the formation of spermatid and efferent duct were observed at 28 dph. At 6 weeks of age, the ovary exhibited yolk granulation in many oocytes, while testis possessed a considerable number of spermatogonia and spermatids. The first ovulation was observed in 9-week-old females, and at the same age, males contained fully-matured spermatozoa. Data obtained in this study indicate that the gonad differentiation of O. dancena is the typical type of differentiated gonochorism.

• Fisheries and Aquatic Sciences
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• 제14권1호
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• pp.43-54
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• 2011

Gene structure of copper/zinc-superoxide dismutase (Cu/Zn-SOD; sod1) was characterized in Hemibarbus mylodon (Teleostei; Cypriniformes), an endangered freshwater fish species in Korean peninsula. Full-length cDNA of H. mylodon SOD1 consisted of a 796-bp open reading frame sequence encoding 154 amino acids, and the deduced polypeptide sequence shared high sequence homology with other orthologs, particularly with regard to metal-coordinating ligands. Genomic structure of the H. mylodon sod1 gene (hmsod1; 1,911 bp from the ATG start codon to the stop codon) was typical quinquepartite (i.e., five exons interrupted by four introns); the lengths of the exons were similar among species belonging to various taxonomic positions. The molecular phylogeny inferred from sod1 genes in the teleost lineage was in accordance with the conventional taxonomic assumptions. 5'-flanking upstream region of hmsod1, obtained using the genome walking method, contained typical TATA and CAAT boxes. It also showed various transcription factor binding motifs that may be potentially involved in stress/immune response (e.g., sites for activating proteins or nuclear factor kappa B) or metabolism of xenobiotic compounds (e.g., xenobiotic response element; XRE). The hmsod1 transcripts were ubiquitously detected among tissues, with the liver and spleen showing the highest and lowest expression, respectively. An experimental challenge with Edwardsiella tarda revealed significant upregulation of the hmsod1 in kidney (4.3-fold) and spleen (3.1-fold), based on a real-time RT-PCR assay. Information on the molecular characteristics of this key antioxidant enzyme gene could be a useful basis for a biomarker-based assay to understand cellular stresses in this endangered fish species.

• Korean Journal of Ichthyology
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• 제17권4호
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• pp.248-254
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• 2005

Integumentary structure of the amberjack, Seriola dumerili, was examined by light and transmission electron microscopy. Integument of the amberjack consists of epidermal and dermal layers. The epidermal thickness is about $28.32{\mu}m$. The epidermal layer could be classified into superficial, intermediate, and basal layers by morphology and position of the supporting cells. Secretory cells of the epidermal layer were classified into mucous cells and club cells in the amberjack. Mucous cells contained acidic mucous and were alcian-blue positive with AB-PAS (pH 2.5) reaction. The club cells contained well-developed rough endoplasmic reticula and large central vacuole. Also, filament-rich cells were identified in the epidermal layer. The dermal layer consists mainly of collagenous fiber, and it contains fibrocytes and pigment cells. The cytoplasm of fibrocyte had a well-developed rough endoplasmic reticulum. The pigment cells contained electron-dense melanin granules.

• Applied Microscopy
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• 제28권3호
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• pp.273-282
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• 1998

Ultrastructure of fertilized egg envelope in the tomato clown anemonefish (Amphiprion freatus) was examined by mean of light, scanning and transmission electron microscopies. The fertilized egg of tomato clown anemonefish was of non-transparent, ellipsoidal, adhesive and non-floted type, and there were numerous oil droplets in the yolk sac and adhesive filaments in the area of the animal pole. The outer surface of egg envelope was smooth. The fertilized egg envelope consisted of two distinct layers; a non-adhesive outer electron-dense layer and an inner layer, consisting of five horizontal low electron-dense lamellae alternating with the middle electron dense interlamellae.

• Fisheries and Aquatic Sciences
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• 제7권3호
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• pp.136-140
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• 2004

We examined the ovaries of 312 robust tonguefish (Cynoglossus robustus) collected each month from January to December 2001, off the coast of Yeosu, Korea. The samples were used to investigate robust tonguefish reproductive activity. Monthly changes in the gonadosomatic indexindicated that spawning peaked between June and August. Fish size at first spawning was 15-19 cm total length (TL). Over $50{\%}$ of female fish 25-29 cm TL were sexually mature, and this rose to $100{\%}$ for female fish 35-39 cm TL. The tonguefish spawned four or more times during the spawning season. Most females commenced spawning in their second year. The relationship between total length (cm TL) and fecundity (F) was F=528646Ln(TL)-1E+06.

• Korean Journal of Ichthyology
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• 제17권2호
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• pp.98-104
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• 2005

Integumentary structures of the chub mackerel (Scomber japonicus) were examined by light and transmission electron microscopy. The integument of the fish was composed of epidermal and dermal layers. The epidermal thickness is about $20{\mu}m$ in TL 35~40 cm fish. The epidermal layer could be classified into superficial, intermediate, and basal layers by morphology and position of the supporting cells. The epidermal layer also possessed mucous cells, club cells and chloride cells. The area of secretory cells, including mucous and club cells, is about 23% of the epidermal layer. The mucous materials were identified as sulfated glycoprotein, neutral and acid in nature. Club cells had a large central vacuole and rough endoplasmic reticula in the cytoplasm. Chloride cells had numerous tubular mitochondria in the cytoplasm. The dermal layer consists mainly of collagenous fiber, and it contains fibrocytes, pigment cells and cycloid scales.

• Fisheries and Aquatic Sciences
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• 제10권4호
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• pp.196-199
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• 2007

The spermatozoa of Leiocassis nitidus are relatively simple cells composed of a spherical head, a short midpiece, and a tail, as in most Siluriformes. The ultrastructure is characterized by the following features: Acrosome absent, as in most teleosts; around nucleus about $1.8\;{\mu}m$ long, with a deep nuclear fossa containing the proximal and distal centrioles and mitochondria. Two centrioles approximately $180^{\circ}$ from each other; 10 or more mitochondria surrounding the axoneme (with a 9+2 microtubular pattern), arranged in two layers in the postnuclear cytoplasm and separated from the axoneme by the cytoplasmic canal. Two lateral fins on the same plane as the two central microtubules; doublets 3 and 8, which are ultrastructural characteristics of the sperma tail unlike other siluroids laking the lateral fins.

• Korean Journal of Fisheries and Aquatic Sciences
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• 제37권6호
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• pp.505-510
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• 2004

The feeding habits of brown barracuda (Sphyraena pinguis) were analyzed using the stomach contents of monthly samples collected from coastal waters of Gadeok-do, Korea from January to December, 1998. Brown barracuda occurred mainly from summer to autumn in the study area, and ranged from 15 to 35 cm in standard length. Brown barracuda was a piscivorous fish which, mainly consumed teleost fish such as anchovy (Engranlis japonicus) and chefoo thryssa (Thfssa kammalensis). Its diet also included small quantities of shrimp and cephalopods. Although smaller fishes (<25 cm SL) consumed shrimp and cephalopods as well as fish, larger fishes (>30 cm SL) consumed exclusively teleost fish. The prey size increased with increase of fish size.