• Journal of Microbiology and Biotechnology
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• 제19권11호
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• pp.1342-1347
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• 2009

We have isolated endophytic fungi from the Indian yew tree, Taxus baccata, and then screened for taxol production. Out of the 40 fungal cultures screened, one fungus Gliocladium sp. was found to produce taxol and 10-DABIII (10-deacetyl baccatin III). These compounds were purified by TLC and HPLC and characterized using UV-spectroscopy, ESI-MS, MS/MS, and proton NMR. One liter of Gliocladium sp. culture yielded $10\;{\mu}g$ of taxol and $65\;{\mu}g$ of 10-DABIII. The purified taxol from the fungus showed cytotoxicity towards cancer lines HL-60 (leukemia), A431 (epidermal carcinoma), and MCF-7 (breast cancer).

• Mycobiology
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• 제39권3호
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• pp.151-157
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• 2011

The endophytic fungal populations of different tissues of Taxus baccata grown at high altitudes in West Bengal, India were explored. These isolated fungal populations represented different genera, which were screened for taxol production using immunoassay technique. The culture AAT-TS-$4_1$ that produced taxol was identified as Gliocladium sp. based on its cultural, morphological characteristics, internal transcribed spacer, and 18S rRNA sequence analysis. Kinetics of taxol production as a function of culture growth were investigated.

• Animal Systematics, Evolution and Diversity
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• 제18권1호
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• pp.85-89
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• 2002

깡충좀벌과의 무늬날개깡충좀벌속(Epitetracnemus)을 우리나라에서 처음으로 보고하며, 또한 이 속에 속하는 1신종, E. kosef sp. nov.를 기재·보고한다.

• Journal of Microbiology and Biotechnology
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• 제8권5호
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• pp.427-440
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• 1998

• Mycobiology
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• 제50권5호
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• pp.317-325
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• 2022

Endophytic fungi occupy various ecological niches, which reinforces their diversity. As few studies have investigated the endophytic fungi of alpine conifers, we focused on four species of alpine conifers in this study-Abies nephrolepis, Pinus pumila, Taxus cuspidata var. nana, and Thuja koraiensis-and examined them for endophytic fungi. A total of 108 endophytic fungi were isolated. There were four taxa in A. nephrolepis, 12 in P. pumila, 18 in T. cuspidata var. nana, and 17 in T. koraiensis; these were divided into five classes: Agaricomycetes (3.2%), Dothideomycetes (29.0%), Leotiomycetes (15.0%), Sordariomycetes (41.9%), and Orbiliomycetes (1.6%). The most prevalent fungi were Sydowia polyspora (22.7%) and Xylariaceae sp. (22.7%) in P. pumila, Phomopsis juglandina (16.1%) in T. cuspidate var. nana, and Thuja-endophytes sp. 1 (70.0%) in T. koraiensis. However, there was no dominant species growing in A. nephrolepis. Some host plants were analyzed using next-generation sequencing. We obtained 4618 reads for A. nephrolepis and 2268 reads for T. koraiensis. At the genus level, the top three endophytic fungi were Ophiostomataceae_uc (64.6%), Nectriaceae_uc (15.5%), and unclassified organism (18.0%) in A. nephrolepis and Nectriaceae_uc (41.9%), Ophiostomataceae_uc (41.8%), and Magnaporthaceae_uc (9.2%) in T. koraiensis. Our results show that there are different communities of endophytic fungi among different host plants, even if the host plants are in the same region. Such ecological niches are important in terms of the ecological restoration of alpine conifers.

• 식물병연구
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• 제22권4호
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• pp.243-248
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• 2016

As preventive measures for bonsai exports, nematodes were isolated from 55 bonsai samples of five coniferous species (Chamaecyparis pisifera, Juniperus chinensis, Pinus densiflora, Pinus thunbergii, and Taxus cuspidate) from all 15 bonsai gardens in Korea. Nematodes belonging to 21 genera in 7 orders were isolated from the 55 bonsai samples. Among plant-parasitic nematodes, Tylenchus spp. was the most frequently isolated (14.9%), followed by Ditylenchus spp. (10.5%), Aphelenchoides spp. (9.5%), Aphelenchus sp. (5.5%), Criconemoides sp. (4.0%), Helicotylenchus sp. (0.7%), Hemicycliophora sp. (0.7%), Mesocriconema sp. (0.7%), Tylenchorhynchus sp. (0.7%), and Paratylenchus sp. (0.4%). Among nonparasitic nematodes, Cephalobina was the most frequently isolated nematodes (26.5%), followed by Rhabditida (19.3%), Dorylaimida (17.8%), Pangrolaimida (14.5%), Plectida (6.5%), Tryphylida (6.2%), Mononchida (3.3%), Alaimida (2.9%), Monhysterida (2.5%), and Triplonchida (0.4%). Based on these results, we conclude that there is no problematic plant-parasitic nematode in bonsai gardens of Korea.

• 한국토양동물학회지
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• 제6권1_2호
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• pp.37-44
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• 2001

제주시의 한라수목원과 서귀포시의 한라수목원 직영 육묘장에서 1999년과 2001년 수목의 뿌리를 가해하는 굼벵이의 종류를 조사하였다. 1999년 3월에는 한라수목원에서 12과 14종의 수종을 대상으로 조사를 하였고, 서귀포 육묘장에서는 주목 묘목에서 굼벵이를 조사하였다. 2001년에는 3월과 9월 한라수목원에서 27과 55종의 수목을 대상으로 조사를 하였으며, 서귀포 육묘장에서는 9과 18종의 수목에서 조사를 하였다. 한라수목원에서는 6종의 굼벵이와 풍뎅이가 채집이 되었는데 큰다색풍뎅이 (Holotrichia niponensis)는 21과 28종의 수목에서 채집되었고, 참검정풍뎅이 (H.diomphalia)는 20과 27종에서 채집이 되었으며, 미동정 굼벵이가 19과 27종의 수목에서 채집되었다. 애우단풍뎅이(Maladera orientalis)는 4과 4종의 수목에서 채집되었고, 똥풍뎅이 1종(Aphodius sp.)과 주둥무늬차색풍뎅이는 석류(Punica granatum)와 참개암나무(Carpinus sieboldiana)에서만 성충으로 채집되었다. 서귀포시의 육묘장에서는 큰다색풍뎅이가 6과 9종의 묘목에서 채집되었고, 참검정풍뎅이는 6과 10종의 묘목에서 채집되었다. 후피향나무(Ternss-traermia japonica)에서는 별줄풍뎅이(Minela testaceopes) 유충이 채집되었다. 큰다색풍뎅이와 참검정풍뎅이, 미동정 굼벵이 1종이 우점종이었으며 녹나무(Cinumomum cam-phrora)에서는 이들이 모두 피해를 주고 있었고, 주목에서는 큰다색풍뎅이의 피해가 심각하였다.