• Journal of Microbiology
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• v.40 no.4
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• pp.335-339
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• 2002

Partial sequences of 16S rRNA gene of five chroococcalian blue-green algal strains, Aphanothece nidulans KCTC AG10041, Aphanothece naegelii KCTC AG10042, Microcystis aeruginosa KCTC AG10159, Microcystis ichthyoblabe KCTC AG10160, and Microcystis viridis KCTC AG10198, which were isolated from water from the Geumgang River, were determined and were inferred their phylogenetic and taxonomic positions among taxa of order Chroococcales. Most taxa of Chroococcales whose partial 16S rRNA gene sequences were aligned in this study, are clustered with other related taxa. Aphanothece nidulans KCTC AG10041 and Aphanothece naegelii KCTC AG10042 made a cluster with other European species of these genera, which supported 100% of the bootstrap trees with a very high sequence similarity (97.4-99.4%) in this study. Three strains, Microcystis aeruginosa KCTC AG10159, M. ichthyoblabe KCTC AG10160, and M. viridis KCTC AG10198, formed a cluster with other Microcystis spp. supported 100 % of the bootstrap trees with a similarity of 97.0-99.9% except for two strains. However, this phylogentic tree made no resolution among the species of Microcystis spp. The topology of the tree reconfirmed the taxonomic status of three species of Microcystis, identified in this study based on the morphology, as three colonial types of Microcystis aeruginosa com. nov. Otsuka et al. (1999c). The genera of chroococcalian cyanophytes are heterogeneously clustered in these sequence analyses. We suggest that more molecular studies on the genera of Chroococcales with reference strains, widely collected from restricted geographic or environmental ranges, get accurate taxonomic or phylogenetic determinations.

• Mycobiology
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• v.44 no.3
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• pp.146-154
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• 2016

The wood decay fungi are a diverse taxonomic group that plays a pivotal role in forest carbon cycling. Wood decay fungi use various enzymatic pathways to digest dead or living wood in order to obtain carbon and other nutrients and these enzymatic systems have been exploited for both industrial and medical applications. Over 600 wood decay fungi species have been described in Korea; however, the recent application of molecular markers has dramatically altered the taxonomy of many of these wood decay fungi at both the genus and species levels. By combining molecular methods, specifically sequences of the internal transcribed spacer region, with traditional morphological characters, this study identified five new species records for Korea in five genera: Aurantiporus, Favolus, Neofavolus, Loweomyces, and Hymenochaetopsis. Three of these genera (Aurantiporus, Favolus, and Loweomyces) were previously unknown in Korea. The relatively simple morphology of the wood decay fungi often leads to ambiguous taxonomic assignment. Therefore, molecular markers are a necessary component of any taxonomic or evolutionary study of wood decay fungi. Our study highlights the need for a more robust and multifaceted approach in investigating new wood decay fungi in Korea.

• ALGAE
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• v.31 no.4
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• pp.317-340
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• 2016

The Abrolhos Continental Shelf (ACS) encompasses the largest and richest coral reefs in the southern Atlantic Ocean. A taxonomic study of non-geniculate coralline algae (NGCA) from the region was undertaken using both morpho-anatomical and molecular data. Specimens of NGCA were collected in 2012 and 2014 from shallow reefs of the ACS. Phylogenetic analysis was performed using dataset of psbA DNA sequences from 16 specimens collected in the ACS and additional GenBank sequences of related NGCA species. Nine common tropical reef-building NGCA species were identified and described: Hydrolithon boergesenii, Lithophyllum kaiseri, Lithophyllum sp., Lithothamnion crispatum, Melyvonnea erubescens, Pneophyllum conicum, Porolithon onkodes, Sporolithon ptychoides, and Titanoderma prototypum. A key for species identification is also provided in this study. Our molecular phylogenetic analyses suggest that Lithophyllum sp. corresponds to a new species. Our study also confirms that Lithophyllum kaiseri is a new record in Brazil. The psbA sequences of Lithophyllum kaiseri and Melyvonnea erubescens matched with type specimens indirectly. The taxonomic identification of the remaining species was supported by morpho-anatomical evidences as DNA sequences of their types or topotypes remain unavailable.

• Journal of Veterinary Science
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• v.22 no.3
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• pp.37.1-37.14
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• 2021

Background: Ticks are one of the most common external parasites in dogs, and are associated with the transmission of a number of major zoonoses, which result in serious harm to human health and even death. Also, the increasing number of pet dogs and pet owners in China has caused concern regarding human tick-borne illnesses. Accordingly, studies are needed to gain a complete understanding of the bacterial composition and diversity of the ticks that parasitize dogs. Objectives: To date, there have been relatively few reports on the analysis of the bacterial community structure and diversity in ticks that parasitize dogs. The objective of this study was to investigate the microbial composition and diversity of parasitic ticks of dogs, and assessed the effect of tick sex and geographical region on the bacterial composition in two tick genera collected from dogs in China. Methods: A total of 178 whole ticks were subjected to a 16S ribosomal RNA (rRNA) next generation sequencing analysis. The Illumina MiSeq platform targeting the V3-V4 region of the 16S rRNA gene was used to characterize the bacterial communities of the collected ticks. Sequence analysis and taxonomic assignment were performed using QIIME 2 and the GreenGene database, respectively. After clustering the sequences into taxonomic units, the sequences were quality-filtered and rarefied. Results: After pooling 24 tick samples, we identified a total of 2,081 operational taxonomic units, which were assigned to 23 phyla and 328 genera, revealing a diverse bacterial community profile. The high, moderate and low prevalent taxa include 46, 101, and 182 genera, respectively. Among them, dominant taxa include environmental bacterial genera, such as Psychrobacter and Burkholderia. Additionally, some known tick-associated endosymbionts were also detected, including Coxiella, Rickettsia, and Ricketssiella. Also, the potentially pathogenic genera Staphylococcus and Pseudomonas were detected in the tick pools. Moreover, our preliminary study found that the differences in microbial communities are more dependent on the sampling location than tick sex in the tick specimens collected from dogs. Conclusions: The findings of this study support the need for future research on the microbial population present in ticks collected from dogs in China.

• Korean Journal of Plant Taxonomy
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• v.37 no.3
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• pp.275-308
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• 2007

For 20 taxa of Korean Allium, including 16 species and 5 varieties, examined were morphological characters (i.e. sexuality, structure and shape of underground part, types and growing patterns of leaf and scape, and shapes of perianth, filament and pistil). The specialization and the evolutionary trends of taxonomic characters were inferred from morphological examination. Taxonomic relationships and system of Korean Allium were also studied. The characters showing evolutionary trends were the structure and shape of underground part including rhizome and bulb, leaf, scape, inflorescens, filament and ovary. It seemed that thin and short rhizome developed from thick and long one, and the membranous simple bulb tunic evolved into fibrous reticulate one. The presence of hyaline sheath in A. monanthum of sect. Microscordum was apomorphic. Both angular leaf blade with 2-rowed vascular bundle and flat blade with 1-rowed vascular bundle were developed from terete one with 2-rowed vascular bundle. The base of filament have differentiated from entire to toothed, and 2-ovuled ovary as well as erect scape before flowering was plesiomorphic type. In addition, sexuality, structure of underground part, the presence of hyaline sheath and cross-section structure of leaf were taxonomic characters with the level of the subgenus in this genus. Shape of rhizome, bulb, leaf, scape and pedicel discriminated each section from other ones in the subgenera easily. The shape and arrangement of perianth and filaments were the diagnostic characters for species level along with shape of ovary and stigma.

• Korean Journal of Plant Resources
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• v.12 no.1
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• pp.50-61
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• 1999

The leaf morphological and anatomical characters about 17 taxa of Aster and its allied taxa were investigated to estimate taxonomic values. Leaf shapes of the treated taxa were divided into five types; elliptic, spathulate, lanceolate, linear, ovatodeltoid. These types were fixed in same taxa, but variable among different taxa, therefore useful as taxonomic character. Leaf margins were divided into four types; entire, serrate, dentate, incised, these types were invariable in most taxa, but variable among individual in same taxa such as Kalimeris incise, Aster spathulifolius. The size and shape of leaf epidermal cell, the size and distributional numbers per 10$\textrm{mm}^2$ of stomata, deposit feature of cuticle were not distinguished clearly from treated taxa, but presence of stomata on adaxial surface, size and distributional numbers per 10$\textrm{mm}^2$ of stomata were useful taxonomic characters in some taxa such as Gymnaster koraiensis, Aster altaicus var. uchiyamae, Aster tripolium, Heteropappus arenarius. The leaf trichomes were divided into five types by basic form, sculpturing on their outer surface and cell arrangement; uniseriate granulate conical type, uniseriate psilate conical type, uniseriate psilate filiform type, globular type, biseriate vesicular capitate type. In spite of various habitat, basic morphology of trichomes were not changed, therefore, it was thought to be good taxonomic character.

• Korean Journal of Environment and Ecology
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• v.29 no.1
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• pp.69-80
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• 2015

Studies on Trapa of Korean, Japanese, and Chinese origins have been generally limited to currently living Trapa, thereby demonstrating the lack of phylogenetic approaches. In the present study, we conducted a numerical taxonomic study on 34 Korean origin Trapa species newly discovered in peat deposits. By using the numerical taxonomy as well as geological data, we created an appearance map based on the periods and taxonomic groups of the Korean Trapa. Further, by using this appearance map, we reviewed the limitations that exist in the taxa and simultaneously discussed the adaptational environment of Trapa in each period. Although other studies have discussed new variants of Trapa worldwide, including T. litwinowii V. vassil. var. chihuensis S.F.Guan & Q.Lang of China (Guan and Lang, 1987), the present study is significant because it provides data on the origin of Korean Trapa species. The availability of such data is essential in determining the adaptational environment of the presently living Trapa. To our knowledge, this is the first report of approximately 10 newly discovered and other presently living species of Trapa. Further, the appearance map in the present study allows the use of the 10 newly discovered species and variants of 34 Trapa species indigenous to South Korea for over 1000 years, for a comparison with the currently living Trapa species. Thus, the present study results could serve as important basic data to understand the adaptational environment of the genus Trapa. from the past to the present in Korea.

• Journal of Plant Biology
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• v.33 no.1
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• pp.1-24
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• 1990

This study was attempted to clarify the taxonomic problems that have been raised up to now in the study o section Telephium, genus, Sedum: the delimitation of taxa belong to Telephium group, their relationship, and nomenclatural confusion and the rank of Telephium group. Specimens were collected at 19 sites in Korea. The habitat, distribution, external morphological characters of habit, leaf shape, phyllotaxy, inflorescence, color of floral organs, floral formula, carpel, capsule, and micro-characters of pollen, seed coat, stigma and anther with SEM were examined. Through this study, the following results were obtained. The delimitation of taxa that belong to section Telephium in Korea and their relationship Nomenclatural errors were corrected. In S. rotundifolium Lee, species epithet“rotundifolium”is later homonym of s. rothundifolium Lamarck, so corrected to S. duckbongii Chung and Kim by article 64 of ICBN. Phyllotaxy was elucidated to meaningless, character in sect. Telephium. S. taquetii was a separate species from S. viridescens and not of its synonym. S. alboroseum Baker in Korea was S. erythrostictum sensu Master not of S. erythrostictum Miquel. By this study , taxa of sect. Telephium in Korea were arranged to 7 species. Considering interspecific relationship of sect. Telephium, S. duckbongii and S. alboroseum are considered to be more advanced forms and S. viridescens, S. taquetii, and S. viviparum to be more primitive forms among 7 species.

• International Journal of Industrial Entomology and Biomaterials
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• v.33 no.2
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• pp.72-77
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• 2016

The genus Scutellathous Kishii, 1955 is a small group known from Korea, Japan, and Taiwan. We carried out a taxonomic study on the Korean species of Scutellathous for the first time. As the result, S. porrecticollis (Lewis, 1894) is revealed to be commonly distributing in Korea and Japan. Additionally, we found that the previous record of Athous jactatus (Lewis, 1894) from North Korea is misidentification of S. porrecticollis. We herein provide redescription of S. porrecticollis and the result of DNA barcoding of congeners belonging to Scutellathous.

• Animal Systematics, Evolution and Diversity
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• v.5 no.2
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• pp.257-268
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• 1989

Hada(1936) reported thelist of 14 species of Korean freshwater Rotifer for the first time. Since his report, there have been no taxonomic studies on the Korean except some sporadical reports which contained lists of species as a partial result of limnological study. The author made a list of 145 species of Korean freshwater Rotifera reported until 1988 with reference as convenient compendium for further taxonomic research.