• Molecular & Cellular Toxicology
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• v.1 no.2
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• pp.92-98
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• 2005

DNA microarray analysis of gene expression in toxicogenomics typically requires relatively large amounts of total RNA. This limits the use of DNA microarray when the sample available is small. To confront this limitation, different methods of linear RNA amplification that generate antisense RNA (aRNA) have been optimized for microarray use. The target preparation strategy using amplified RNA in DNA microarray protocol can be divided into direct-incorporation labeling which resulted in cDNA targets (Cy-dye labeled cDNA from aRNA) and indirect-labeling which resulted in cRNA targets (i.e. Cy-dye labeled aRNA), respectively. However, despite the common use of amplified targets (cDNA or cRNA) from aRNAs, no systemic assessment for the use of amplified targets and bias in terms of hybridization performance has been reported. In this investigation, we have compared the hybridization performance of cRNA targets with cDNA targets from aRNA on a 10 K cDNA microarrays. Under optimized hybridization conditions, we found that 43% of outliers from cDNA technique and 86% from the outlier genes were reproducibly detected by both targets hybridization onto cDNA microarray. This suggests that the cRNA labeling method may have a reduced capacity for detecting the differential gene expression when compared to the cDNA target preparation. However, further validation of this discordant result should be pursued to determine which techniques possesses better accuracy in identifying truly differential genes.

• Journal of Korean Elementary Science Education
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• v.24 no.4
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• pp.345-350
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• 2005

The purpose of this study was to identify observation methods, observation targets and their changes in elementary school students' observing aquariums. Silurus microdorsalis, Orthrias nudus, Iksookimia rotundicaudata and Semisulcospira bensoni in two aquariums were observed for 40 minutes in every week during 10 weeks by elementary children. Students' observation methods and targets were analyzed with their observation diary. Also the change of observation methods and targets on each species were analysed based on the observation diary. This study found the facts which was change of observation methods and observation targets according to observable theme of each species as time goes by. The results also found children's various methods and targets in observing living objects and observation methods were changed variously during 10 week-observation. The results of this study may provide more various and systematic norm and assessment criteria for teaching observation.

• Journal of Microbiology and Biotechnology
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• v.31 no.4
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• pp.621-629
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• 2021

Shigella flexneri is a facultative intracellular pathogen that causes bacillary dysentery in humans. Infection with S. flexneri can result in more than a million deaths yearly and most of the victims are children in developing countries. Therefore, identifying novel and unique drug targets against this pathogen is instrumental to overcome the problem of drug resistance to the antibiotics given to patients as the current therapy. In this study, a comparative analysis of the metabolic pathways of the host and pathogen was performed to identify this pathogen's essential enzymes for the survival and propose potential drug targets. First, we extracted the metabolic pathways of the host, Homo sapiens, and pathogen, S. flexneri, from the KEGG database. Next, we manually compared the pathways to categorize those that were exclusive to the pathogen. Further, all enzymes for the 26 unique pathways were extracted and submitted to the Geptop tool to identify essential enzymes for further screening in determining the feasibility of the therapeutic targets that were predicted and analyzed using PPI network analysis, subcellular localization, druggability testing, gene ontology and epitope mapping. Using these various criteria, we narrowed it down to prioritize 5 novel drug targets against S. flexneri and one vaccine drug targets against all strains of Shigella. Hence, we suggest the identified enzymes as the best putative drug targets for the effective treatment of S. flexneri.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.38 no.4
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• pp.11-21
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• 2015

In order to achieve success in ground operations, searching for moving targets is one of critical factors. Usually, the system of searching for adversary ground moving targets has complex properties which includes target's moving characteristics, camouflage level, terrain, weather, available search time window, distance between target and searcher, moving speed, target's tactics, etc. The purpose of this paper is to present a practical quantitative method for effectively searching for infiltrated moving targets considering aforementioned complex properties. Based upon search theories, this paper consists of two parts. One is infiltration route analysis, through terrain and mobility analysis. The other is building dynamic probability maps through Monte Carlo simulation to determine the prioritized searching area for moving targets. This study primarily considers ground moving targets' moving pattern. These move by foot and because terrain has a great effect on the target's movement, they generally travel along a constrained path. With the ideas based on the terrain's effect, this study deliberately performed terrain and mobility analysis and built a constrained path. In addition, dynamic probability maps taking terrain condition and a target's moving speed into consideration is proposed. This analysis is considerably distinct from other existing studies using supposed transition probability for searching moving targets. A case study is performed to validate the effectiveness and usefulness of our methodology. Also, this study suggests that the proposed approach can be used for searching for infiltrated ground moving target within critical time window. The proposed method could be used not only to assist a searcher's mission planning, but also to support the tactical commander's timely decision making ability and ensure the operations' success.

• Korean Management Science Review
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• v.25 no.1
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• pp.55-74
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• 2008

In recent warfare, the performance improvement of air weapon systems enables an aircraft to strike multiple targets on a single sortie. Further, aircrafts attacking targets may carry out an operation as a strike package that is composed of bombers, escort aircrafts, SEAD (Suppression of Enemy Air Defenses) aircrafts and etc. In this paper, we present an aircraft allocation model that allocates multiple targets to a single sortie in the form of a strike package. A mixed integer programming is developed and solved by using a commercially available software. The new model is better than existing ones because not only it allocates aircrafts to multiple targets but also it models the concept of the strike package. We perform a computational experiment to compare the result of the new model with that of existing ones, and perform sensitivity analysis by varying a couple of important parameters.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.882-887
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• 2005

The paper describes Quasi-simultaneous recognition of plural targets and motion control of robot based on the recognition. The method searches for targets by model-based matching method using the hybrid GA, and the motion of the robot is generated based on the targets' positions on the image. The method is applied to a soccer robot, and targets are a ball, a goal, and an enemy in the experiment. The Experimental results show robustness and reliability of the proposed method.

• Proceedings of the KIEE Conference
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• 2005.11a
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• pp.62-64
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• 2005

Indium Tim Oxide(ITO) thin film was prepared for TOLEDs by Facing Targets Sputtering(FTS) apparatus which can suppress the damage of organic layer due to the collisions of high energetic particles. In particular, ITO thin film was prepared with changing the distance between two targets for reduced the bombardment by high energetic particles such as ${\gamma}-electron$ or negative oxygen ions. The electrical and optical properties of ITO thin films as a function of distance of between two targets were measured. Additionally, the ITO thin films were prepared on the cell (cell : MgAg/LiF/EML/HTL/ bottom electrode) with distance of between two targets. And the I-V characteristics of ITO/cell was investigated.

• Proceedings of the KIEE Conference
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• 1998.07g
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• pp.2430-2432
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• 1998

Generally, in the best-known Pulse-type type and Frequency Modulated Continuous Wave(FM/CW)-type RF range measurement systems, the nearest measurable distance between antenna of system and targets is limited to several tens of meters. Moreover, in case of high-speed targets it is more difficult to measure the distance precisely. In this paper we design our own RF(X-band) range(up to 15 meters) measurement system usi code correlation for high- speed targets. It 0 the correlation value between transmitting receiving PN (Pseudo -Noise) codes. So we determine the distance between antenna of s and targets using this correlation value. We fabricated it using MIC techniques and experimental results show that the proposed syste fully qualified for a short range measurement syste

• Proceedings of the KIEE Conference
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• 2001.07d
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• pp.2417-2419
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• 2001

A path planning is one of the main subjects in a mobile robot. It is divided into two parts. One is a global path planning and another is a local path planning. This paper, using the formal two methods, presents that the mobile robot moves to multi-targets with avoiding unknown obstacles. For the shortest time and the lowest cost, the mobile robot has to find a optimal path between targets. To find a optimal global path, we used GA(Genetic Algorithm) that has advantage of optimization. After finding the global path, the mobile robot has to move toward targets without a collision. FLC(Fuzzy Logic Controller) is used for local path planning. FLC decides where and how faster the mobile robot moves. The validity of the study that searches the shortest global path using GA in multi targets and moves to targets without a collision using FLC, is verified by simulations.

• Journal of the Korean institute of surface engineering
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• v.33 no.4
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• pp.229-232
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• 2000

C-axis oriented zinc oxide thin films were deposited on glass substrate by reactive Facing Targets Sputtering (FTS) system. The characteristics of zinc oxide thin films on power, inter targets distance, and substrate temperature were investigated by XRD(x-ray diffractometer), alphastep (Tencor) analyses. The Facing Targets Sputtering system can deposit thin film in plasma-free situation and change the deposition condition in wide range. The excellently c-axis oriented zinc oxide thin films were obtained at sputter pressure 1mTorr, sputtering current 0.4A, substrate temperature $300^{\circ}C$, inter targets distance 100mm. In the conditions, the rocking curve of zinc oxide thin films deposited on ZnO/Glass was $3.9^{\circ}$.