• 한국환경보건학회지
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• 제46권1호
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• pp.45-64
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• 2020

Objectives: Limited information is available on the presence and associated ecological risks of pharmaceutical residues in aquatic environments near pharmaceutical manufacturing areas in Korea. In this study, we investigated the current state of pharmaceutical contamination and its associated ecological risks in streams near a pharmaceutical manufacturing complex. Methods: Seven pharmaceuticals (acetaminophen, clarithromycin, diclofenac, diphenhydramine, ibuprofen, mefenamic acid and roxithromycin) were measured in water samples collected from the streams near a pharmaceutical manufacturing complex. A predicted no-effect concentration (PNEC) was derived using either the assessment factor method or species sensitivity distribution method. In addition, a hazard quotient for each pharmaceutical was calculated by dividing its measured environmental concentration by its PNEC. Results: Samples collected downstream from the wastewater treatment plant (WWTP) had higher concentrations of pharmaceuticals than those collected from the reference site (upstream). Moreover, pharmaceutical concentrations were greater in ambient water than in the final effluent from the WWTP, which suggested that non-point sources were contributing to the contamination of the ambient water environment. Some of the target pharmaceuticals exhibited a hazard quotient >1, indicating that their potential ecological effects on the aquatic environment near the pharmaceutical industrial area should not be ignored. Conclusion: This study demonstrated that the pharmaceutical manufacturing area was contaminated with residual drugs, and that there was a possible non-point source near the WWTP effluent discharge area. The results of this study will aid in the development of management plans for pharmaceuticals, particularly in hotspots such as pharmaceutical industrial sites and their vicinities.

• Parasites, Hosts and Diseases
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• 제58권3호
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• pp.301-308
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• 2020

Owing to global climate change, the global resurgence of vector-borne infectious diseases and their potential to inflict widespread casualties among human populations has emerged as a pivotal burden on public health systems. Tsutsugamushi disease (scrub typhus) in the Republic of Korea is steadily increasing and was designated as a legal communicable disease in 1994. The disease is a mite-borne acute febrile disease most commonly contracted from October to December. In this study, we tried to determine the prevalence of tsutsugamushi disease transmitted by chigger mites living on rodents and investigated their target vector diversity, abundance, and distribution to enable the mapping of hotspots for this disease in 2015. A total of 5 species belonging to 4 genera (109 mites): Leptotrombidium scutellare 60.6%, L. pallidum 28.4% Neotrombicula tamiyai 9.2%, Euschoengastia koreaensis/0.9%), and Neoschoengastia asakawa 0.9% were collected using chigger mite collecting traps mimicking human skin odor and sticky chigger traps from April to November 2015. Chigger mites causing tsutsugamushi disease in wild rodents were also collected in Hwaseong for the zoonotic surveillance of the vector. A total of 77 rodents belonging to 3 genera: Apodemus agrarius (93.5%), Crocidura lasiura (5.2%), and Micromys minutus (1.3%) were collected in April, October, and November 2015. The most common mite was L. pallidum (46.9%), followed by L. scutellare (18.6%), and L. orientale (18.0%). However, any of the chigger mite pools collected from rodent hosts was tested positive for Orientia tsutsugamushi, the pathogen of tsutsugamushi disease, in this survey.

• 토지주택연구
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• 제6권4호
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• pp.221-229
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• 2015

아파트 단지 내 대형교목은 공동주택 외부환경의 질적 수준을 좌우하는 주요한 요인이다. 이것은 단지의 랜드 마크적 활용 및 공간 상징성 제고를 위한 목적으로 활용된다. 그러나 대형교목의 식재는 높은 유지관리비용 및 하자의 발생으로 오히려 외부환경의 질적 저하를 초래하는 요인이 되기도 한다. 이에 본 연구는 공동주택 인공지반에 식재되는 대형 교목을 대상으로, 식재실태를 분석하여 대형교목의 식재 개선방향 제시를 목적으로 한다. 이를 위해 3개 대상지의 대형교목(R20이상) 총 265주를 대상으로, 조경설계계획 및 현장설계 변경사항, 그리고 준공실태를 관련 설계도서 및 현장 조사를 기반으로 식재 현황 및 최소 생육 토심, 추가 토심조치 유형 등을 조사하였다. 그 결과, 공동주택의 대형교목 조경설계는 85% 심근성 수종으로 평균 R35의 대형교목이 인공지반에 식재되고, 평균 65cm 최소 생육 토심이 부족한 것으로 조사되었다. 그리고 현장설계변경은 주로 R30과 R40의 특정 규격에 한정된 검토가 이루어지고, 부족 토심에 대한 추가계획 등은 수반되지 않고 있는 것으로 조사되었다. 다음으로, 추가 토심 확보방안으로 85%가 마운딩으로 조치하였으나, 만족율은 10%내외로 매우 낮아, 조치방법에 문제가 있음을 지적하였다. 또한 대형 교목의 규격과 뿌리근 특성 이외에, 식재 입지선정이 최소 생육 토심 확보에 주요한 요인임을 지적하고, 식재계획 시 개선방안을 제시하였다.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.179-188
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• 2017

White-rot basidiomycetes are the organisms that decompose lignin most efficiently, and Trametes villosa is a promising species for ligninolytic enzyme production. There are several publications on T. villosa applications for lignin degradation regarding the expression and secretion of laccase and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breakdown of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA, and the corresponding lignin peroxidase (TvLiP) protein from T. villosa strain CCMB561 from the Brazilian semiarid region. The presence of ligninolytic enzymes produced by this strain grown in inducer media was qualitatively and quantitatively analyzed by spectrophotometry, qPCR, and dye fading using Remazol Brilliant Blue R. The spectrophotometric analysis showed that LiP activity was higher than that of MnP. The greatest LiP expression as measured by qPCR occurred on the $7^{th}$ day, and the ABSA medium (agar, sugarcane bagasse, and ammonium sulfate) was the best that favored LiP expression. The amplification of the TvLiP gene median region covering approximately 50% of the T. versicolor LPGIV gene (87% identity); the presence of Trp199, Leu115, Asp193, Trp199, and Ala203 in the translated amplicon of the T. villosa mRNA; and the close phylogenetic relationship between TvLiP and T. versicolor LiP all indicate that the target enzyme is a lignin peroxidase. Therefore, T. villosa CCMB561 has great potential for use as a LiP, MnP, and Lac producer for industrial applications.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 춘계학술대회
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• pp.148-148
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• 2003

This study is to investigate the environmental levels and trend of dioxins, which was the 3$\^$rd/ year of environmental monitoring research for endocrine disrupting chemicals since 1999. Total 282 samples were analyzed from 115 sites including 26 sites of airs, 43 sites of waters, 11 sites of sediments and 35 sites of soil, which were the same as those of investigated sites in 2000. Sampling period was from June 2001 to June 2002. Target chemicals were seventeen species of 2,3,7,8-chlorine-substituted PCDD and PCDF congeners and were analyzed by the standard methods, established by National Institute Environmental Research (NIER). The average concentration of dioxins in air decreased from 0.324 pg-TEQ/N㎥ in 2000 to 0.287 pg-TEQ/N㎥ in 2001, and those in water and soil were 0.073pg-TEQ/L and 1.703pg-TEQ/dry g, respectively, which was the less values detected in 2000. In sediment, however, the value was 0.086pg-TEQ/dry g, which was the increase from the value of the year 2000. The concentration range of dioxins in air for 26 sites in 17 regions detected were 0.013∼l.664pg-TEQ/N㎥, 4 sites from those were exceeded the Air Quality Standards of Dioxin in Japan (0.6 pg-TEQ/N㎥). The tolerable daily intake of dioxins was calculated at the highest level (1.664) in air, with referring the soil and food data from Japan, was calculated to be 2.85pg-TEQ/kg/day, which was below the level of 4 pg-TEQ/kg/day suggested in KFDA(Korea). While the average concentration of dioxins in 15 big cities was 0.190 pg-TEQ/N㎥, that in 8 medium/small cities constituting an industrial complex was 0.558 pg-TEQ/N㎥. In water, the concentration range detected were 0∼0.946pg-TEQ/L and the trend of the average concentrations shows an increase from those of 1999 but decreased from those of 2000, any sites however were not exceeded the Water Quality Standards of Dioxin in Japan (1 pg- TEQ/L). In soil. the detected range were 0∼43.333 pg-TEQ/dry g and the average concentration decreased, compared with the results of 2000. According to the monitoring results by land utilization, the detected range were 0∼43.333pg-TEQ/dry g in farmland, 0.017∼0.601 pg-TEQ/dry g in the industrial area, 0.005∼0.049pg-TEQ/dry g in the park and 0.008∼1.825 pg-TEQ/dry g in the rest. In sediment, the detected range increased from 0∼0.244 pg-TEQ/dry g to 0∼0.537 pg-TEQ/dry g, based on the results of 2000. For the proper control of dioxins, continuous monitoring needs to be performed and in addition, the dioxin inventory should be prepared for major sources through the dioxin emission survey. These results would provide sound and solid basis for proper decision making of dioxins management like establishment of environmental quality standards in Korea.

• 한국환경복원기술학회지
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• 제20권6호
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• pp.117-131
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• 2017

This study was conducted to draw vegetation landscape elements in the ancient literature, investigate plant community structure, understand vegetation structure, and suggest reasonal conservation management methods. As a result of analyzing ancient literature, geomorphological landscapes in Guryong valley were canyon landscape and valley surrounded in Mt. Chiak. Plant landscape factors were as followed. Rhododendron spp. spread around valley. Also, Pinus densiflora communities were distributed in Guryong valley around. Especially, the entrance zones of Guryong valley were described as covered with Pinus densiflora and Whangchangkumpyo(黃腸禁標). Therefore, it was estimated that entry of Guryong valley was covered with Pinus densiflora community landscape. As for current vegetation result, the main vegetation was divided into mixed deciduous broad-leaved trees community and Pinus densiflora community. As a result of analysis by TWINSPAN for community classification, five communities(Deciduous broadleaved trees, Pinus densiflora, Pinus densiflora-Abies holophylla, Abies holophylla, and Pinus koraiensis community) were classified. To maintain historic plant landscape and conserve crucial resources, Pinus densiflora community was classified as concentrated conservation management area($105,472m^2$). To maintain Pinus densiflora landscape which has high historical and cultural value of Guryong valley, it was considered that active density control of lower layer vegetation would be necessary. Accordingly, to conserve P. densiflora landscape, Whangchangkumpyo(?腸禁標), that area was separated into Pinus densiflora lower layer forest management area($84,029m^2$) and Pinus densiflora seedling conservation management area($21,443m^2$). In understory of Pinus densiflora lower layer flora, the target tree species for elimination and management were Quercus serrata and Quercus mongolica. They were $4{\sim}6trees/100m^2$ and their average diameter was 7.1cm. To preserve Pinus densiflora seedlings, areas with Sasa borealis, the ground vegetation of Pinus densiflora community, rate of 80% or more should be selected as priority management areas and concentrated elimination and management of Sasa borealis should be implemented. Likewise, traditional Pinus densiflora forest is a historically cultural heritage to preserve with sustainable interest and survey. Efficient management method through systematic monitoring system should be made.

• The Korean Journal of Physiology and Pharmacology
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• 제15권1호
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• pp.61-66
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• 2011

P2Y receptors are metabotropic G-protein-coupled receptors, which are involved in many important biologic functions in the central nervous system including retina. Subtypes of P2Y receptors in retinal tissue vary according to the species and the cell types. We examined the molecular and pharmacologic profiles of P2Y purinoceptors in retinoblastoma cell, which has not been identified yet. To achieve this goal, we used $Ca^{2+}$ imaging technique and western blot analysis in WERI-Rb-1 cell, a human retinoblastoma cell line. ATP ($10\;{\mu}M$) elicited strong but transient $[Ca^{2+}]_i$ increase in a concentration dependent manner from more than 80% of the WERI-Rb-1 cells (n=46). Orders of potency of P2Y agonists in evoking $[Ca^{2+}]_i$ transients were 2MeS-ATP>ATP>>UTP=${\alpha}{\beta}$-MeATP, which was compatible with the subclass of $P2Y_1$ receptor. The $[Ca^{2+}]_i$ transients evoked by applications of 2MeS-ATP and/or ATP were also profoundly suppressed in the presence of $P2Y_1$ selective blocker (MRS 2179; $30\;{\mu}M$). $P2Y_1$ receptor expression in WERI-Rb-1 cells was also identified by using western blot. Taken together, $P2Y_1$ receptor is mainly expressed in a retinoblastoma cell, which elicits $Ca^{2+}$ release from internal $Ca^{2+}$ storage sites via the phospholipase C-mediated pathway. $P2Y_1$ receptor activation in retinoblastoma cell could be a useful model to investigate the role of purinergic $[Ca^{2+}]_i$ signaling in neural tissue as well as to find a novel therapeutic target to this lethal cancer.

• 한국산학기술학회논문지
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• 제21권8호
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• pp.417-428
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• 2020

하우스키핑 유전자는 에너지생성, 물질합성, 세포사멸 및 세포방어 등과 같은 세포의 기본적인 기능을 수행하기 때문에 모든 유기체의 세포에서 발현된다. 세포의 기본적인 기능을 유지하기 때문에 발현 수준이 상대적으로 일정하여 단백질 발현 및 목적 유전자의 mRNA 발현 분석 등과 같은 유전자 발현 연구에서 기준 유전자로 사용되고 있다. 그러나 이들 유전자의 발현 수준은 조직과 세포마다 다를 수 있으며, 특정 환경 하에서 변할 수 있다. 그러므로 하우스키핑 유전자의 발현 안정성을 탐색하여 유전자 발현 연구에서 최적의 기준 유전자를 선택하는 것이 중요하다. 이 리뷰는 문헌을 통해 인간, 닭, 돼지 그리고 쥐에서 발견된 하우스키핑 유전자를 요약하고, geNorm, NormFinder 그리고 BestKeeper 소프트웨어를 통해 발현 안정성을 추정하였다. 하우스키핑 유전자의 발현 안정성에 대한 탐색은 유전자 발현 연구에서 실험 조건에 따라 가장 적합한 기준 유전자를 선별할 수 있고, 데이터의 정규화를 위해 적용될 수 있다.

• 대한환경공학회지
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• 제37권6호
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• pp.349-356
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• 2015

본 연구는 부산연근해에서 서식하는 저서어류 체내의 미량금속에 의한 인체 위해 영향을 정량적으로 평가하기 위하여 수행되었다. 부산의 용호부두 인근에서 채취 된 4종의 식용저서어류 넙치, 우럭, 쥐치, 밑달갱이 조직내의 미량금속(trace metals)의 함량을 ICP-MS를 이용하여 분석하였다. 그리고 이를 토대로 어류섭취를 통한 미량금속의 인체 노출량을 파악하고, 이로 인한 잠재적 인체 위해도 평가를 수행하였다. 4종의 어류 조직 내 미량금속의 평균함량은 Li, Cr, Ni, Cu, Zn, As, Cd, Pb이 각각 $0.005{\pm}0.009$, $0.77{\pm}0.30$, $0.29{\pm}0.34$, $0.49{\pm}0.14$, $15.96{\pm}2.52$, $10.62{\pm}4.67$, $0.001{\pm}0.002$, $0.045{\pm}0.06mg/kgdw$으로 나타났다. 이 지역에서 채취된 어류의 섭취로 인한 표준체중(60 kg)의 국민에 대한 노출평가 결과 Cu, Zn은 PMTDI (provisional maximum tolerable daily intake)의 0.0032, 0.054-0.18%, As, Cd, Pb는 PTWI (provisional tolerable weekly intake)값의 13, 0.0041, 0.020%로 나타나 합동식품첨가물전문가위원회(The Joint FAO/WHO Expert Committee on Food Additives, JFCFA)에서 권고하는 각원소의 제한섭취량에 비하여 매우 낮은 수준이었다. 그리고 독성(비발암)위해도 평가 결과는 모두 HI (hazard index)지수가 1 이하로 장기간 섭취하여도 안전한 것으로 나타났으며, 4종의 모든 어류섭취로 인한 As의 노출로 인한 초과 발암 위해도도 10만명 당 1명 이하로 나타나 역시 장기섭취로 인한 발암 확률도 무시할 만한 수준인 것으로 나타났다.

• 대한환경공학회지
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• 제30권1호
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• pp.45-53
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• 2008

본 연구에서는 국내 한 정수장의 고도정수처리공정의 실험분석 자료를 바탕으로 정수장으로 유입되는 원수의 수질특성과 처리공정별 오염물질 제거효율의 변화특성을 연구하였고, 활성탄여과조의 역세척에 따른 BAC 내의 미생물 거동을 추적하였다. KMnO$_4$ 소비량 등 8$\sim$9개의 주요 항목을 중심으로 제거특성이 연구되었고, 활성탄여과조의 유입 유출수에 대하여 NH$_3$-N 등 4항목의 농도변화를 추적하였다. 역세척 전 후의 활성탄 시료의 SEM 사진을 촬영하였고, 미생물 생체량(HPC) 조사를 통해 활성탄 부착미생물의 존재를 확인하고자 하였다. 유입원수의 특성은 대부분의 조사항목이 일정한 농도범위로 유입되었고 그 중 계절적 일관성을 갖는 항목이 있었다. 공정별 제거효율 변화특성은 월별 일정한 유형을 갖는 것, 공정진행에 따라 지속적으로 제거되는 것, 오존접촉조에서 제거효율이 떨어지는 것 등으로 분류되었고, 현재 운전되고 있는 고도처리공정에서 제거되는 주요 대상오염물질을 구별할 수 있었다. NH$_3$-N의 농도변화 곡선으로 활성탄여과조 내에 미생물이 존재함을 예측하여 SEM 사진과 HPC 측정으로 이를 확인하였다.