• Asian-Australasian Journal of Animal Sciences
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• 제24권11호
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• pp.1529-1540
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• 2011

Silencing of a specific gene using RNAi (RNA interference) is a valuable tool for functional analysis of a target gene. However, information on RNAi for analysis of gene function in farm animals is relatively nil. In the present study, we have validated the interfering effects of siRNA (small interfering RNA) using both quantitative and qualitative gene silencing in buffalo granulosa cells. Qualitative gene knockdown was validated using a fluorescent vector, enhanced green fluorescence protein (EGFP) and fluorescently labeled siRNA (Cy3) duplex. While quantitatively, siRNA targeted against the luciferase and CYP19 mRNA was used to validate the technique. CYP19 gene, a candidate fertility gene, was selected as a model to demonstrate the technique optimization. However, to sustain the expression of CYP19 gene in culture conditions using serum is difficult because granulosa cells have the tendency to luteinize in presence of serum. Therefore, serum free culture conditions were optimized for transfection and were found to be more suitable for the maintenance of CYP19 gene transcripts in comparison to culture conditions with serum. Decline in fluorescence intensity of green fluorescent protein (EGFP) was observed following co-transfection with plasmid generating siRNA targeted against EGFP gene. Quantitative decrease in luminescence was seen when co-transfected with siRNA against the luciferase gene. A significant suppressive effect on the mRNA levels of CYP19 gene at 100 nM siRNA concentration was observed. Also, measurement of estradiol levels using ELISA (enzyme-linked immunosorbent assay) showed a significant decline in comparison to control. In conclusion, the present study validated gene silencing using RNAi in cultured buffalo granulosa cells which can be used as an effective tool for functional analysis of target genes.

• 비교문화연구
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• 제22권
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• pp.223-277
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• 2011

Tan Yuanjie(譚元杰) of CHINA COSTUME ART OF PEKING OPERA("中國京劇服裝圖譜") is 'Foreword' attention from the bar 'Formalism'. A note is makeup system from ever performances here, 'what kind of adaptation must be a corresponding type of costume should be worn.' This stance to 'type of person's identity and faced the scene correlated' with the actual performance tradition plays out is going and, while here the rules to capture the opera's on the character of 'identity and the circumstances under clothing' is defined. This position discussed previously 'Formalism' in line with the will he perform, and looks to meet the elements of production. This basic stance is clean up, while 'Old Beijing Opera costumes costumes taxonomy largely' literary costume' and 'militant outfit' into two groups divided over throughout steamroll surgery, because surely need to have a more systematic classification. The classification system was established as 'Part 1. Mang, Part 2. Pi, Part 3. Xi, Part 4. Kao, Part 5. YI'. In addition to these classification systems, as well as the aforementioned 'object theory' Given the symbolic significance of the capacity to keep in mind is necessary. Costumes conduct, character, situation, atmosphere and so the transport of charged symbols here, a target symbol of the system is the projection of water. This costume is detrimental to the mall for the positionsay, but I kept in mind damwongeolyi internationalization of Chinese culture. when you see the view from the perspective of semiotic systems for the sign, that the theater is necessary to complement. In this paper, 'Yi(衣)' costume on the corresponding point of the target compared to the China Culture Department of Theatre and Folklore methodology ran off and sprinting was to lay the groundwork for research.

• 한국의상디자인학회지
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• 제17권3호
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• pp.197-206
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• 2015

Ghetto was originated from the concept of "Jewish Camp", the segregated Jewish residential area, and recently it refers to the black neighborhoods of the poor living conditions or slum. A region to form a unique culture distinguished from the adjacent area is also called as ghetto. The culture born in that region is called "ghetto culture", and from the cultural aspect it can mean a type of haven that allows the freedom and deviation of their own. In this study, the generating background and the characteristics of ghetto style especially to adolescents were examined, and celebrities' unique fashion styles that lead the public in the fashion diffusion process were analyzed. Through this, Ghetto culture was understood and the effects of the mass culture phenomena on fashion, symbolization, and an aesthetic value were examined. With this, it aims to help understand the effect of special local culture like "ghetto" on modern fashion and expand the design area. As a result of this study, the characteristics of ghetto style were as followings; 1. It is based on hiphop style; 2. The name brands are exposed conspicuously; 3. Caught eyes by unusually excessive decorations; 4. It expresses confidence and toughness through fashion beyond the resistance to the target who suppresses and humiliates themselves; 5. Ghetto culture is rapidly spread through media. To conclude, ghetto style is an expression of hope of the poor that they can gain wealth through impressing the public and drawing empathy just by their talent. Furthermore, ghetto style is an important cultural trend that has appeared as their wannabe and a powerful display method to express success.

• BMB Reports
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• 제49권3호
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• pp.149-158
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• 2016

Plants have evolved a vast chemical cornucopia to support their sessile lifestyles. Man has exploited this natural resource since Neolithic times and currently plant-derived chemicals are exploited for a myriad of applications. However, plant sources of most high-value natural products (NPs) are not domesticated and therefore their production cannot be undertaken on an agricultural scale. Further, these plant species are often slow growing, their populations limiting, the concentration of the target molecule highly variable and routinely present at extremely low concentrations. Plant cell and organ culture constitutes a sustainable, controllable and environmentally friendly tool for the industrial production of plant NPs. Further, advances in cell line selection, biotransformation, product secretion, cell permeabilisation, extraction and scale-up, among others, are driving increases in plant NP yields. However, there remain significant obstacles to the commercial synthesis of high-value chemicals from these sources. The relatively recent isolation, culturing and characterisation of cambial meristematic cells (CMCs), provides an emerging platform to circumvent many of these potential difficulties.

• 농촌계획
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• 제19권1호
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• pp.165-177
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• 2013

This study deals with rural village development project of Gasangri, Yeongcheon city area, based on the use of the cultural factors and strengthening them in study area. Unlike other cases of today's Korean rural village development projects, this plan keenly concerns not only for tourists but also for the village people. Since culture means the quality of life also, this project especially deals with 'community', 'well-being', and 'convenience' for the inhabitants. On the other hand, it attempts to connect with "Cyan Museum", which is the first grade art gallery remodelled from closed school, to make village of culturally abundant atmosphere. In result, seven sub-projects of the expansion of basic living foundation, and two improvement of landscape in area have planned. Among stereotype of rural development projects and similar programs of tourism, the project makes difference on targeting for the meaning and resource of place culture.

• 복식문화연구
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• 제29권2호
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• pp.289-306
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• 2021

The study aims to define the Korean Wave as global attention to Korea's unique culture and consider the specificity of traditional Korean fashion images in Korean Wave content. The research method of this study is a case study through literature research. In order to collect Korean Wave content on YouTube, 24 channels with the highest number of views were selected from among content uploaded from 2018 to the present through keyword search, and up to two channels with high views showing traditional Korean fashion images. As a result of selecting the analysis target, 41 Korean Wave videos and 368 costumes were selected and analyzed based on fashion style elements, including item, color, detail, motif, styling, silhouette, and accessory. As a result of the study, music, broadcast, fashion, and other content were found in the Korean Wave content fields in which Korean fashion style appeared, and the characteristics of each field were derived. Music content was characterized by fashion style excluding stereotypes about traditional Korean costume, broadcast and fashion content was characterized by fashion style inherited from traditional costume, and other content was characterized by symbolic fashion style of traditional culture. This study is meaningful in revealing the formative characteristics of traditional Korean design elements recently shared online through the study of Korean traditional fashion images in Korean Wave content.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제17권12호
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• pp.3364-3382
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• 2023

Remote sensing image segmentation plays an important role in realizing intelligent city construction. The current mainstream segmentation networks effectively improve the segmentation effect of remote sensing images by deeply mining the rich texture and semantic features of images. But there are still some problems such as rough results of small target region segmentation and poor edge contour segmentation. To overcome these three challenges, we propose an improved semantic segmentation model, referred to as MRU-Net, which adopts the U-Net architecture as its backbone. Firstly, the convolutional layer is replaced by BasicBlock structure in U-Net network to extract features, then the activation function is replaced to reduce the computational load of model in the network. Secondly, a hybrid multi-scale recognition module is added in the encoder to improve the accuracy of image segmentation of small targets and edge parts. Finally, test on Massachusetts Buildings Dataset and WHU Dataset the experimental results show that compared with the original network the ACC, mIoU and F1 value are improved, and the imposed network shows good robustness and portability in different datasets.

• 패션비즈니스
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• 제16권2호
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• pp.44-61
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• 2012

The 21st century directs designomics to achieve wealth by designing old beyond a simple recognition of the importance of culture. One of the representative local cultures of Gyeongnam is Gaya culture. The Gaya period had over 600 years history with rich and excellent iron manufacturing skill and advanced pottery culture. It is the very time to reevaluate the importance of Gaya equivalent to the Three Kingdoms in our ancient history which was has been so far ignored and isolated. In order to develop unique and excellent culture of Gaya as local fashion cutural goods, questionnaire survey on the recognition of Gaya culture and cultural goods purchasing status had been conducted to target visitors of Representative National Museum. Data Analysis Using SPSS 18.0 Win was performed with frequency analysis and t-test. As to the questionnaire survey, important criteria for purchasing in case of buying cultural goods could be found and the residents in Gyeongnam had higher recognition of developing cultural goods by the motif of Gaya relics. 'Mounted vessel in the shape of warrior on horseback(12.4%)' and 'Chariot wheel-shaped Pottery(10.8%)' were selected the most suitable motif, 'Cellphone strap(11.0%)' and 'key holder(10.2%), 'cup (7.2%)'were selected the most suitable item of cutural goods. These will be actively reflected in the development of fashion cultural goods design applying Gaya relics.

• 설비공학논문집
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• 제23권3호
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• pp.231-242
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• 2011

This study examines influences of a daylight dimming control system on the variation of indoor illuminance and lighting energy savings in a small office space. Field measurements and a series of computation were performed for typical types of sky conditions in summer. Results indicate that the daylight illuminance under clear and partly-cloudy sky were close to the target illuminance during a majority of time due to a higher ratio of window to wall. However, the target illuminance was not kept successfully due to the variation of photosensor signals which were strongly influenced by desktop illuminance. The system with partially-shielded conditions succeeded to keep target illuminance under clear sky conditions. The system failed under overcast sky conditions since the electric light output from fixtures caused excessive signals to photosensors due to insufficient daylight on a desktop. Unshielded and fully-shielded conditions were not recommended for effective controls of the systems. The influence of lighting fixtures on photosensors should be minimized to achieve successful lighting controls by daylight dimming systems.

• 산업식품공학
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• 제21권3호
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• pp.191-200
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• 2017

There have been great efforts to develop a rapid and sensitive detection method to monitor the presence of pathogenic bacteria in food. While a number of methods have been reported for bacterial detection with a detection limit to a single digit, most of them are suitable only for the bacteria in pure culture or buffered solution. On the other hand, foods are composed of highly complicated matrices containing carbohydrate, fat, protein, fibers, and many other components whose composition varies from one food to the other. Furthermore, many components in food interfere with the downstream detection process, which significantly affect the sensitivity and selectivity of the detection. Therefore, isolating and concentrating the target pathogenic bacteria from food matrices are of importance to enhance the detection power of the system. The present review provides an introduction to the representative sample preparation strategies to isolate target pathogenic bacteria from food sample. We further describe the nucleic acid-based detection methods, such as PCR, real-time PCR, NASBA, RCA, LCR, and LAMP. Nucleic acid-based methods are by far the most sensitive and effective for the detection of a low number of target pathogens whose performance is greatly improved by combining with the sample preparation methods.