• International Journal of Oral Biology
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• 제45권3호
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• pp.134-142
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• 2020

Colon cancer is one of the most common malignant tumors, but there are still a few validated biomarkers of colon cancer. Exosome-mediated microRNAs (miRNAs) have been recognized as potential biomarkers in cancers, and miRNAs can regulate a variety of genes. Recently, Fusobacterium nucleatum was discovered in the tissues of human colon cancer patients. Its role in colon cancer was highlighted. F. nucleatum may contribute to the progression of colon cancer through the mechanism of exosome-mediated miRNAs transfer. However, the exosomal miRNAs regulation mechanism by F. nucleatum in colon cancer is not well known. Thus, we performed next-generation sequencing to investigate the overall pattern of exosomal miRNAs expression in the colon cancer cell culture supernatant. We have confirmed the alterations of various exosomal miRNAs. In addition, to investigate the function of exosomal miRNAs, a Kyoto Encyclopedia of Genes and Genomes analysis was performed on the target genes of changed miRNAs. Potential target genes were associated with a variety of signaling pathways, and one of these pathways was related to colorectal cancer. These findings suggested that F. nucleatum can alter exosomal miRNAs released from colorectal cancer cells. Furthermore, exosomal miRNAs altered by F. nucleatum could be potential biomarkers for the diagnosis and therapy of colon cancer.

• 복식
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• 제66권1호
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• pp.58-72
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• 2016

Since the mid 2000s, a large number of Chinese students have come to Korea to study. This study investigates the research trends of Chinese students studying in the fashion field in Korea. For this study, a total of 235 graduate degree theses on fashion written by Chinese students in Korea (from 1992 to 2014) were collected through the RISS website. Various keywords were used to find the theses, including fashion, clothing and costume. Factors used in the analysis of these theses were the number of theses per year, major of the student, research trend of specific areas and research target area. The results are as follows: Most of the theses were written by Fashion/Clothing majors (141 theses/60% of the total), while other majors - such as Business Administration, International Trade, Economics, Journalism/Broadcasting, and Movie Entertainment - made up the rest (94/40%). The theses researched in the study were focused on a specific field in fashion. Fashion Marketing/Socio-Psychology of Clothing was the most popular field (113/48.1%), and Fashion Design/Aesthetics came in second (87/37.0%). Other topics, such as Costume History, Clothing Construction/Textile Science, Costume Culture, followed. Chinese student's research target area was very limited, with Chinese Study being the most popular area, and Korean and Chinese Comparative Study coming in second.

• Journal of Microbiology and Biotechnology
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• 제22권6호
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• pp.736-741
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• 2012

In this study, we analyzed the oxidative stress response of wblA ($\underline{w}$hi$\underline{B}$-$\underline{l}$ike gene $\underline{A}$, SCO3579), which was previously shown to be a global antibiotic down-regulator in Streptomyces coelicolor. Ever since a WblA ortholog named WhcA in Corynebacterium glutamicum was found to play a negative role in the oxidative stress response, S. coelicolor wblA has been proposed to have a similar effect. A wblA-deletion mutant exhibited a less sensitive response to oxidative stress induced by diamide present in solid plate culture. Using real-time RT-PCR analysis, we also compared the transcription levels of oxidative stress-related genes, including sodF, sodF2, sodN, trxB, and trxB2, between S. coelicolor wild type and a wblA-deletion mutant in the presence or absence of oxidative stress. Target genes were expressed higher in the wblA-deletion mutant compared with wild type, both in the absence and presence of oxidative stress. Moreover, expression of these target genes in S. coelicolor wild type was stimulated only in the presence of oxidative stress, suggesting that WblA plays a negative role in the oxidative stress response of S. coelicolor, similar to that of C. glutamicum WhcA, through the transcriptional regulation of oxidative stress-related genes.

• 디지털융복합연구
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• 제12권5호
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• pp.451-458
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• 2014

본 논문에서는 관광의 1차적인 정보원으로서의 홈페이지와 관광홍보물의 언어학적 전환의 특징을 살펴보고자 한다. 즉 관광관련 사이트의 한국어 원문과 영어 번역물을 비교하면서 관광정보 텍스트가 올바르게 언어전환 되었는지 점검하고 나아가 전환된 영어번역물과 한국어 원문을 대조하면서 번역물에서 발생하는 언어규약과 의사소통의 특징을 살펴본다. 특히 한국어와 영어는 언어유형에서 대조적인 언어로서 번역을 통해 전환되었을 때 특이한 담화의 양상을 보인다. 즉 한국어에 비해 영어표현은 직설적이고 우회적인 표현을 피하는 경향이 있다. 또한 영어번역본에서는 영어 화자위주로 언어가 변형되어 표현됨을 주시할 수 있다. 이것은 영어를 사용하는 언어군에서 흔히 발생하는 양상으로 광의의 문화간의 차이로 간주될 수 있다. 따라서 외국관광객의 1차적 정보원으로서의 관광웹사이트는 관광정보를 제공하는 동시에 목적지의 문화를 간접적인 형태로 표현함으로써 사회적 이미지 형성과 문화간 차이를 대변하며 커뮤니케이션의 일환으로서 공손의 원칙과 Grice의 원칙을 준수하고 있다.

• 전자공학회논문지SC
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• 제42권6호
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• pp.49-56
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• 2005

개발된 마이크로전자빔 조사장치는 100KeV 이하의 에너지를 갖는 전자를 세포의 핵에 0.2Gy 이하의 양으로 조사한 후 저준위 방사선에 대한 세포의 생물학적 영향을 해석하기 위해 고안된 시스템이다. 방사선의 생물학적 영향 평가에 유의한 통계성을 확보하기 위해서는 조사되는 세포의 수가 10,000개 이상이 되어야하고 실험 세포의 수명 및 방사선 피폭 후 영향 발현 관찰 시기 등을 고려할 때 시간당 10,000개 이상의 세포 조사가 요구된다. 10,000개 이상의 세포 조사를 위해서는 $320{\times}240{\mu}m^2$ 범위 내에 20개 정도의 세포가 분포하는 프레임을 500번 이상 이동하여야 한다. 본 논문에서는 장치 운영을 위한 사용자 인터페이스와 함께 효율적인 조사 알고리즘을 이용한 세포 자동 인식과 위치 제어를 구현하고 검증하였다.

• Biomolecules & Therapeutics
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• 제27권1호
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• pp.117-125
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• 2019

Mebendazole (MBZ), a microtubule depolymerizing drug commonly used for the treatment of helminthic infections, has recently been noted as a repositioning candidate for angiogenesis inhibition and cancer therapy. However, the definite anti-angiogenic mechanism of MBZ remains unclear. In this study, we explored the inhibitory mechanism of MBZ in endothelial cells (ECs) and developed a novel strategy to improve its anti-angiogenic therapy. Treatment of ECs with MBZ led to inhibition of EC proliferation in a dose-dependent manner in several culture conditions in the presence of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) or FBS, without selectivity of growth factors, although MBZ is known to inhibit VEGF receptor 2 kinase. Furthermore, MBZ inhibited EC migration and tube formation induced by either VEGF or bFGF. However, unexpectedly, treatment of MBZ did not affect FAK and ERK1/2 phosphorylation induced by these factors. Treatment with MBZ induced shrinking of ECs and caused G2-M arrest and apoptosis with an increased Sub-G1 fraction. In addition, increased levels of nuclear fragmentation, p53 expression, and active form of caspase 3 were observed. The marked induction of autophagy by MBZ was also noted. Interestingly, inhibition of autophagy through knocking down of Beclin1 or ATG5/7, or treatment with autophagy inhibitors such as 3-methyladenine and chloroquine resulted in marked enhancement of anti-proliferative and pro-apoptotic effects of MBZ in ECs. Consequently, we suggest that MBZ induces autophagy in ECs and that protective autophagy can be a novel target for enhancing the anti-angiogenic efficacy of MBZ in cancer treatment.

• 농업과학연구
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• 제48권2호
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• pp.353-365
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• 2021

Previously, we reported that tissue factor (Tf) was included in the list of differentially expressed genes as an upregulated gene in a rejected porcine heart after xenotransplantation into monkey. In this study, we analyzed that expression of Tf in aortic endothelial cells (pAEC) isolated from alpha 1,3-galactosyltransferase knockout pig in response to allogeneic porcine serum and xenogeneic human serum. The consequence was significant upregulation of Tf expression by responding to human serum compared with porcine serum. To analyze the function of Tf gene as a promoter, we constructed reporter vectors for expression of luciferase linked to 1,246 and 787 base pairs of porcine Tf (pTF1246 and pTF787), and 535 base pairs of human TF (hTF535) sequences including putative promoter regions and AP-1 biding site at the 5' end. The reporter vectors were transfected into pAEC including cytomegalovirus enhancer/chicken β-actin (CAG)-luciferase vector as a control. Luciferase assay showed that all of the promoters were insufficient to express luciferase compared with CAG promoter in basic culture conditions. Notably, pTF1246, pTF787, and hTF535 led to a significant increase of luciferase expression in response to human serum compared with porcine serum while no change of CAG. pTF1246 and pTF787 showed higher expression than hTF535. Taken together, our findings suggest that pTF1246 and pTF787 promoters could mediate target gene expression specifically at xenogeneic stress condition.

• 한국IT서비스학회지
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• 제21권5호
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• pp.51-64
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• 2022

Companies are planning and executing IT projects using information technology as a means to secure external competitiveness. However, IT projects have high risks and uncertainties due to the invisibility of outputs (systems, services, products), and changes in plans frequently occur during project execution. As a result, most IT projects are closed without achieving the target performance. This can lead to a waste of resources and money for the company, which in turn leads to the loss of opportunities to enter new markets. This study intends to analyze the effect of changes in the project plan on customer satisfaction, which is the project performance. Also, we want to find the importance of project planning so that the target performance of the IT project can be achieved. For the empirical analysis of this study, about 500 actual project data were collected. As for the analysis method, statistical analysis such as simple and multiple regression analysis and control effect was performed. Looking at the results of the analysis, it was found that the scope change affects the cost change and the schedule change. Also, changes in scope and cost were found to affect project performance. The theoretical performance of this study proved the theoretical fact that good project performance comes out if the IT project is executed as planned. The practical performance suggested the need for a change in project management by proving that thorough execution of the project planning stage can improve the project performance in the Korean project management culture, where the project planning stage is poorly performed for the rapid implementation of the IT project.

• 한국재난정보학회 논문집
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• 제19권3호
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• pp.490-497
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• 2023

연구목적: 우리나라는 1990년대 이후 중대 산업사고 예방을 위해 유해 위험방지계획서 시행, 공정 안전관리 제도 시행 등 여러모로 노력해 오고 있으나 최근의 일련의 중대 산업사고는 그 한계를 보여주고 있다. 그래서 본 연구는 PSM 대상사업장의 안전관리 개선을 위한 대책을 제시하고자 한다. 연구방법: 산업재해 현황, 선행연구조사, PSM 대상사업장 재해 현황과 사고 발생 현황, PSM 대상사업장 안전관리 개선에 관한 선행연구와 사례를 조사하여 기존의 안전관리를 기반으로 PSM 대상사업장 안전관리에 대한 개선방안을 수립하였다. 연구결과: PSM대상사업장의 안전관리 개선을 위한 조직문화구축, 재난발생시 대응시스템, 체계적 안전관리, 노후설비교체 및 점검강화, 안전에 대한 경영자의 확고한 의지등의 대책을 제시하였다. 결론: 본 연구를 통하여 소중한 생명과 피해를 줄이기위한 안전확보 체계화방안을 제시한 것으로 의미를 가지며 각 사업장별로 PSM개선방향을 고찰하는데 활용성을 가질것으로 기대한다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.285-285
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• 2022

Recent unpredictable climate change is a major cause of rice yield loss. In particular, methane is a key factor in global warming. Therefore rice breeders are trying to breed the reducing-methane gas emission rice using the crossbreeding method. However, the traditional crossbreeding method takes 8 to 10 years to breed a cultivar, and the anther culture method developed to shorten the breeding cycle also takes 6 to 7 years. On the other hand, CRISPR/Cas9 accurately edits the target trait and can rapidly breed rice cultivars by editing the target trait as a homozygous in 2-3 years. In addition, exogenous genetic elements such as Cas9 can be isolated from the G₁ generation. Therefore, the flowering time was regulated by applying CRISPR/Cas9 technology, and OsCKq1 genome-editing (OsCKq1-G) rice with early flowered and high yield was bred in the field. Genome-editing of OsCKq1 applied CRISPR/Cas9 technology up-regulates the expression of the flowering promotion gene Ehd1 under long-day conditions induces early flowering and increases the yield by increasing the 1,000-grain weight. And as the generations advanced, each agricultural trait indicated a low coefficient of variation. As a result, indicated that OsCKq1 plays an important role in regulating the flowering time and is related to the trait determining yield. Therefore, OsCKq1-G can suggest a breeding strategy for the Net-Zero national policy for reducing-methane gas emission rice by shortening the breeding cycle with the early flowered, and high-yield rice. CRISPR/Cas9 technology is a rapid and accurate breeding technology for breeding rice cultivars with important characteristics.