• 한국생물공학회:학술대회논문집
• /
• 2000.11a
• /
• pp.651-654
• /
• 2000

A fermentation study of the recombinant Escherichia coli system has been tired to overproduce a recombinant Paragonimus westermani cysteine proteinase, rPwCP1. Using the modified LB media, main cultures and chemical induction with IPTG were carried out to examine the possibility of secretory protein overproduction at high cell density culture. As a result, the target protein of rPwCP1, purified by metal affinity chromatography and gel filtration, has been shown at 50.8 kDa on SDS-PAGE, and its final concentration turns out to be 350mg/L.

• Journal of Information Technology Applications and Management
• /
• v.21 no.1
• /
• pp.1-16
• /
• 2014

Cyberloafing is as counterproductive behavior because it is the act of using internet for personal purposes which can affect employees' productivity, especially when done excessively (more than 4 hours a week). The objective of this study was to analyze whether gender and education level had significant effect on cyberloafing among generations in the workplace. From 267 respondents, gender was not found to have impact on cyberloafing because female and male respondents had the same frequency of low level cyberloafing. However, female had higher excessive cyberloafing than male. Gen Y had significant impact on the relationship between education and cyberloafing but Gen Y with diploma degree was revealed to have excessive cyberloafing. Furthermore, Gen X had started combining professional and personal lives throughout their daily lives. Hence, organizations should foster a culture of hard work by giving challenging job with clear target and worthy incentive but still implementing internet usage policy.

• Journal of Environmental Health Sciences
• /
• v.29 no.3
• /
• pp.1-8
• /
• 2003

The development of environmentally nontoxic or non-polluting antifouling additives that can be formulated in practical coating requires assay involving target organisms. Described here are the simple laboratory assays that have been developed using the barnacle, Balanus amphitrite, a common fouling organism found throughout temperate and tropical seas. One of the assays depends on synchronous year-round mass culture, the procedure for which is described, of nauplii larvae and cyprids larvae. The laboratory assays provided quantitative estimates of toxicity and settlement inhibition of the test compounds. Laurinterol (1), isolaurinterol (2), alpysinal (3), and aplysin (4) have been isolated from the Korean red alga Laurencia okamurae. Their structures were identified by spectral data in comparison with the literature data. Compounds 1-4 inhibited larval settlement of the barnacle B. amphitrite with EC$_{50}$ values of 0.18- 36 $\mu\textrm{g}$/ml. Com-pounds 2-4 showed larval toxicity against nauplii of the barnacle B. amphitrite with 5-10 $\mu\textrm{g}$/ml, while laurinterol (1) exhibited no toxicity at even 100 $\mu\textrm{g}$/ml. Therefore, laurinterol was expected as a promising natural antifoulant.t.

• Plant Resources
• /
• v.4 no.3
• /
• pp.192-195
• /
• 2001

Human immunodeficiency virus (HIV), the causative agent of AIDS, still continues to spread rapidly in the world population, especially in Africa and Southeast Asia. At present, two kinds of therapeutic approaches are used for treatment of AIDS. One is to target HIV reverse transcriptase, which is responsible for the viral genome transcription. The other is to inhibit HIV pretense PR, which is essential for the processing of viral proteins. Drug combinations based on these approaches can reduce the blood virus to an undetectable level. However, a small amount of virus may lurk inside the immune cells in a dormant state. Another major obstacle of long-term treatment of the disease is remarkable mutation in HIV. Most of the clinical chemotherapeutic agents have one or more of these problems. High cost and harmful side-effects further reduced the desirability of these drugs. In the course our studies on development of anti-HIV agents from natural products, we investigated various crude drugs for their inhibitory activity against HIV-induced cytopathic effects (CPE) in culture cells, HIV-pretense (PR), HIV-reverse transcriptase (RT) including ribonuclease H (RNase H), and HIV integrase (INT). In the present paper, some inhibitory substances relating to the development of anti-HIV agents are reported.

• International Commerce and Information Review
• /
• v.7 no.4
• /
• pp.245-268
• /
• 2005

The purpose of this paper is to acquire competitiveness faced with a global business so that Korea and China make them put ICT into practice through industrial policy of regional innovation clustering. In the Chapter 2, overall review of industrial spaces theory and the environment in Global-business is conducted. In the Chapter 3, current main economic issue and West Great Development of China are viewed. Chapter 4 proposes models and strategies for the target of regional innovation clustering and phasing in development. The results of this study is that both country should do more long-term cooperation and collecting intensive knowledge for the property of region and preparatory research of regional innovation clustering than do reckless investment.

• Journal of Korean Medical classics
• /
• v.19 no.2 s.33
• /
• pp.1-10
• /
• 2006

The main purpose of this essay is to shed light on the foundation of the core notion used in oriental medicine. Under the premise that the important notion of oriental medicine has its origin in the culture of the ancient china before B.C.$2^{\sim}1$, we will get to the source notion of oriental medicine by retrospecting the analogical thinking used in the course of forming the main notion of oriental medicine. For the source notion being in various domains, we must search so many domains for example the political system including the offical system, the economic system and so on. But in this essay, we will limit the domain concerned with combat.

• Journal of Pharmaceutical Investigation
• /
• v.40 no.4
• /
• pp.201-212
• /
• 2010

Self-organized nanogels from polysaccharide derivatives offer a promising approach in treatment of cancer due to their flexibility in chemistry and their ability to improve the therapeutic index of a drug by modifying biodistribution by their preferential localization at target sites and lower distribution in normal healthy tissues. These properties have promoted studies of active cancer targeting by self-organized nanogels for even better accumulation in solid tumors. However although many researchers have reported their potential by using cell culture systems and small animal tumor models in cancer therapy, these nanogels need more decoration such as conjugation with targeting moiety and endowment of stimuli-sensitivity for precise targeting of the cancer site. In this review, we summarize the recent efforts in developing novel targeting approaches via active endocytosis and stimuli-sensitive systems responding to hyperthermic or acidic tumor pH conditions.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1998.11a
• /
• pp.132-132
• /
• 1998

It has been known that MAO (monoamine oxidase) catalyses the oxidation of endogenous neurotransmitter amines. From its key role in the regulation of some physiological amines, it has been the target of inhibitors used as antidepressive agents. In our continuing search for MAO inhibitors from Basidiomycete. sp., strain 8082 was selected. Two metabolites (8082-1 and 8082-2) were isolated by adsorption chromatography and HPLC from the culture broth of strain 8082. The structure of 8082-1 and 8082-2 were elucidated by $^1$H-, $\^$13/C-NMR and HMBC spectral data, and these products were identified as 5-methylmellein and nectriapyrone, respectively, which have significant inhibitory effect against mouse brain MAO.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.47 no.6
• /
• pp.740-744
• /
• 2014

In this study, the ${\beta}$-lactamase (VPA0477) gene was used as a new target for the PCR-based detection of Vibrio parahaemolyticus. Primers specific for the ${\beta}$-lactamase (VPA0477) gene of V. parahaemolyticus, were designed and incorporated into a PCR-based assay. The assay was able to specifically detect all of the 191 V. parahaemolyticus strains tested, but did not result in amplification of 39 other Vibrio spp. and non-Vibrio spp. strains tested. The detection limit of the assay was 10 CFU of V. parahaemolyticus RIMD2210633 from pure culture broth. The ${\beta}$-lactamase (VPA0477) gene-based assay developed in this study was sensitive and specific, and has great potential for the accurate detection and identification of V. parahaemolyticus in seawater or seafood samples.

• English Language & Literature Teaching
• /
• v.13 no.2
• /
• pp.177-200
• /
• 2007

The language socialization approach states that novices are socialized into cultural norms through participating in routine, repeated interactional acts and sequences (e.g., Ochs & Schieffelin, 1984; Ochs, 1988; Schieffelin & Ochs, 1986a; 1986b; Watson-Gegeo & Gegeo, 1986). One of the cultural norms or dominant epistemological orientations in American culture is the tendency to avoid the overt display of power asymmetry in novice-expert relationship (Ochs & Schieffelin, 1984). This study examines how this cultural preference is reflected and encoded in ESL teachers' use of routine discourse patterns in corrective sequences. Eight hours of ESL classes taught by three Caucasian teachers born and educated in the U.S. were analyzed for the study. The analysis showed that the cultural tendency in question is keyed and indexed in the teacher's routine corrective discourse patterns in the form of various questioning, elicitation, and mitigation practices. Findings support that teachers' routine classroom discourse practices represent their cultural ideologies and transfer these cultural predispositions to second language learners and that they possibly socialize the learners into the target language-oriented beliefs.