• Microbiology and Biotechnology Letters
• /
• v.31 no.4
• /
• pp.322-328
• /
• 2003

The Transformation-Associated Recombination (TAR) cloning technique allows selective isolation of chromosomal regions and genes from complex genomes. The procedure requires knowledge of relatively small genomic sequences that reside adjacent to the chromosomal region of interest. This technique involves homologous recombination during yeast spheroplast transformation between genomic DNA and a TAR vector that has 5'and 3' gene targeting sequences. In this study, we examined the minimum size of specific hooks required for a single-copy gene isolation and compared the utility of different TAR vectors, radial and unique vectors, by cloning the same single-copy gene. The efficiency of TAR cloning of the hHPRT gene was same using hooks varying from 750 to 63 bp. The number of transformants decreased approximately 20-fold when the TAR vector contained two unique hooks versus using a radial vector, but the percentage of positive recombinants increased over 2-fold when a unique TAR vector was used. Therefore, we suggest that the two-unique TAR vector is suitable for general TAR cloning given its high selectivity, and the radial TAR vector is more suitable when genomic DNA is in limited quantity, for example, DNA isolated from pathological specimens. Moreover, we confirm the minimal length of a unique sequence in a TAR vector is approximately 60 bp for a single-copy gene isolation.

• Journal of KIISE
• /
• v.45 no.1
• /
• pp.9-14
• /
• 2018

Tar is a Linux command that combines several files into a single file. Combining multiple small files into large files increases the compression efficiency and data transfer speed. However, tar has a problem in that smaller target files, result in a lower performance. In this paper, we show that this performance degradation occurs when tar reads the data from the target files and propose qtar (quick tar) to solve this problem via flash-level remapping. When the size of an I/O request is less than 1 MB, the I/O performance decreases proportionally to the decrease in size of the I/O request. Since tar reads the data of files one by one, a smaller file size results in a lower performance. Therefore, the remapping technique is implemented in qtar to read data from the target files at the maximum I/O size regardless of the size of each file. Our evaluations show that the execution time with qtar is reduced by up to 3.4 times compared to that with tar.

• Applied Chemistry for Engineering
• /
• v.31 no.3
• /
• pp.328-333
• /
• 2020

In this study, blended feedstock derived pyrolyzed fuel oil (PFO) and coal-tar was prepared to produce a pitch by thermal polymerization reaction for manufacturing artificial graphite materials. The aromaticity value of 0.355 and 0.818 was obtained for PFO and coal-tar, respectively. In addition, PFO and coal-tar exhibited the difference tendency of weight loss curve for thermogravimetric analysis, which is related to the structural stability depending on the aromaticity and functional groups. The production characteristics confirmed that the pitch derived PFO showed lower production yield and higher softening point than that using blended feedstock. In particular, when comparing P360 (138.5 ℃) and B420 (141.4 ℃) having similar softening points, the production yields of both pitches exhibited 29.89 and 49.03 wt%, respectively. This is mainly due to the blending of PFO and coal-tar having high pitch polymerization reactivity including a large amount of alkyl groups and coal-tar having high thermal stability. This phenomenon indicated that the increased production yield is because of a synergic effect of both the high reactivity of PFO and thermal stability of coal-tar.

• Journal of Preventive Medicine and Public Health
• /
• v.30 no.1 s.56
• /
• pp.145-155
• /
• 1997

Interview survey and dermatological examination have been performed to investigate the health problems of workers continuously exposed to coal-tar pitch. The phototoxicity of coal-tar pitch was confirmed by the photopatch tests for six healthy adults. The main results are followings; 1. There was no special history of allergic diseases in both the exposed and non-exposed group. 2. The frequency of the phototoxic dermatosis and the coal-tar acne in the exposed group was significantly greate.(p<0.05) than that of the control group. In the exposed group, the phototoxic dermatosis and the coal-tar acne were observed in nine workers(90%) and seven workers(70%), respectively. However, those, disease were not observed in the control group. 3. Five results(83%) were positive to the photopatch test for coal-ta. pitch 48 hours after UVA irradiation. But the lesion was subsiding 72 hours after UVA irradiation. 4. Malignant cancers were not reported among workers ever exposed to coal-tar pitch. In conclusion, it appears that workers exposed to coal-tar pitch have high risks of phototoxic dermatosis and coal-tar acne. A health policy should be provided to prevent phototoxic dermatosis among coal-tar pitch workers. More studies are required to determine malignancy.

• Korean Journal of Materials Research
• /
• v.23 no.5
• /
• pp.276-280
• /
• 2013

Coal tar is the primary feedstock of premium graphitizable carbon precursor. Coal tars are residues formed as byproducts of thermal treatments of coal. Coal tar pitches were prepared through two different heat treatment schedules and their properties were characterized. One was prepared with argon and oxidation treatment with oxygen; the other was prepared with oxygen treatment at low temperature and then argon treatment at high temperature; both used coal tar to prepare coal tar pitches. To modulate the properties, different heat treatment temperatures ($300{\sim}400^{\circ}C$) were used for the coal tar pitches. The prepared coal tar pitches were investigated to determine several properties, such as softening point, C/H ratio, coke yield, and aromaticity index. The coal tar pitches were subject to considerable changes in chemical composition that arose due to polymerization after heat treatment. Coal tar pitch showed considerable increases in softening point, C/H ratio, coke yields, and aromaticity index compared to those characteristics for coal tar. The contents of gamma resin, which consists of low molecular weight compounds in the pitches and is insoluble in toluene, showed that the degree of polymerization in the pitches was proportional to C/H ratio. Using an oxidizing atmosphere like air to prepare the pitches from coal tar was an effective way to increase the aromaticity index at relatively low temperature.

• Proceedings of the Korean Society of Medical Physics Conference
• /
• 2003.09a
• /
• pp.40-40
• /
• 2003

목적 : 현재 국내에서 사용중인 Co-60 원격치료용 방사선 조사장치의 경우 tissue air ratio(TAR)는 조사 표면에서 최대 선량을 가지는 back scatter factor(BSF)를 적용하여 구한 값을 사용하고 있는데, 실제로 Co-60 원격치료용 방사선 조사장치의 최대선량 깊이는 조사 표면이 아니라, 조사 표면에서 0.5cm 떨어진 거리에서 최대 선량을 나타내므로, BJR 25 에서 권장하는 값인 peak scatter factor(PSF)를 이용해 구한 값이 더 정확한 값으로 사료되기 때문에 이를 본 실험을 통해 검증하고자 하였다. 대상 및 방법 : 방사선 종양학과에서 치료용으로 사용하고 있는 Co-60 원격치료용 방사선 조사장치를 대상으로 하였다. BSF 는 Khan이 저술한 The Physics of Radiation Therapy의 부록에 제시된 값을 사용하였으며, PSF와 TAR를 구하기 위해 물 팬톰(water phantom), Farmer형 이온 챔버(ion chamber), 전기계(electrometer)를 사용하였다. PSF와 TAR를 구하기 위해서 몇 가지 측정을 하였다. 먼저, 공기 중에서 챔버를 SSD=80.5cm에 고정시킨 후, 방사선을 조사하여 선량을 측정하고, 깊이에 따른 선량을 알아보기 위해, 물 팬톰 내에 챔버를 SSD=80cm 고정시킨 후, 물을 서서히 채워가면서 5$\times$5cm, 10$\times$10cm, 15$\times$15cm, 20$\times$20cm, 30$\times$30cm의 field size에 대해서, 물의 깊이가 0.5cm-2cm 까지는 0.5cm 단위로 선량을 측정하고, 물의 깊이가 2cm-l4cm까지는 1cm단위로 선량을 측정하였다. 측정된 선량을 이용하여 PSF를 구하고 난 후, BJR 25에서 제시한 PSF와 비교를 하였고 TAR은 Khan이 제시한 변환식에 PSF를 대입하여 알아보았다. 기존의 TAR과 PSF를 이용해 구한 TAR을 측정하여 구한 TAR과 비교하였다. 결과 : BJR25에서 제시한 PSF와 본 실험에서 측정하여 얻은 PSF를 비교한 결과, field size가 5$\times$5cm, 10$\times$10cm, 15$\times$l5cm, 20$\times$20cm인 경우, 측정하여 얻은 PSF가 0.8%, 0.2%, 0.4%, 0.2%로 약간 높지만, 두 값은 매우 유사한 것으로 나타났다. 그리고, 기존의 BSF를 이용해 구한 TAR과 BJR 25에서 권고하는 PSF를 이용해 구한 TAR을 비교한 결과 field size 에 따라 약 1%-1.5% 정도로 BSF를 이용하여 구한 TAR보다 PSF를 이용하여 구한 TAR이 1.3% 정도 높게 나타났지만, 이것은 두 값의 절대적인 차이일 뿐, 실제로는 PSF를 이용하여 구한 TAR이 측정해서 구한 TAR과는 매우 유사한 값을 보여주고 있다. 결론 : 기존의 BSF를 이용해 구한 TAR과 PSF를 이용해 구한 TAR을 비교하였을 때, 약 1.3% 정도 높게 내고 있지만, 기존의 TAR보다는 PSF를 이용해 구한 TAR이 BJR 25와 잘 일치하고 있으므로 Co-60 원격치료용 방사선 조사장치를 사용할 경우 BSF보다는 PSF를 사용하는 것이 타당한 것으로 사료된다.

• Journal of the Korean Society of Combustion
• /
• v.16 no.1
• /
• pp.8-14
• /
• 2011

Biomass gasification is a promising technology in terms of clean energy and flexible options for end use such as heat, steam, electricity, gaseous or liquid fuels. In a gasification process, reduction of tar is very important because it can cause any mechanical problems and small tar implies high energy efficiency. However, generation and conversion mechanisms of tar have not been fully understood due to its complex nature. In this study, characteristics of tar generated from different gasification stages were investigated. Korean pine woodchip was used as feedstock and tar was sampled in a separate way during devolatilization and char gasification stage, investigated. As a result. more various kinds of hydro carbon compounds were identified in the devolatilization stage than char gasification stage because primary tar compounds are released mostly from pyrolysis of cellulose and hemicellulose. When the reaction temperature increased up to $900^{\circ}C$, tar composition becomes simplified into about 10 aromatic compounds mostly with 1-4 rings without substitution up to phenanthrene. The sampled tar in the char gasification stage mostly contains 5-7 simple aromatic compounds.

• Journal of Korean Foot and Ankle Society
• /
• v.15 no.3
• /
• pp.132-138
• /
• 2011

Although first generation total ankle replacement (TAR) had high failure rates, recent investigations have reported good results of the newer generations of TAR due to advances in implant designs and techniques. Patient selection is critical to performing TAR to obtain promising outcomes and to decrease complication rate. As the current concepts of correcting the accompanying deformity have been established, TAR in moderate to severe varus deformity of the ankle result in favorable outcomes and indications for TAR are expanding. Correction of deformity and hindfoot fusion should be performed in conjunction with TAR if needed. If radiolucency around components or osteolysis is progressive during follow-up, CT should be carried out as a confirmative diagnostic method. TAR is an effective treatment modality alternative to ankle fusion. However, we should recognize that TAR is a demanding procedure, which requires accurate techniques, enough experience, and preoperative plan for a concomitant deformity.

• Journal of Energy Engineering
• /
• v.23 no.2
• /
• pp.42-48
• /
• 2014

Soot and tar which were derived from combustion or pyrolysis processes in Puverized Coal(PC) furnace or boiler have been significantly dealing in a radiative heat transfer and an additional source of NOx. Furthermore, the increasing for the use of a coal with low caloric value gives rise to a lot of tar-soot yield and LOI in a recycled ash for using cement materials. So, the ash with higher tar-soot yield and LOI can not recycle due to decreased strength of concrete. In this study, tar-soot yields and flame structures were investigated using the LFR for a blending combustion with bituminous coal and sub-bituminous coal. Also, The investigation were conducted as each single coals and blending ratio. The coals are used in a doestic power plant. In the experimental results, sub-bituminous coal with high volatile contents shows longer soot cloud length than bituminous coal, but overall flame length was shorter than bituminous coal. Tar-soot yields of sub-bituminous coal is lower than those of bituminous coal. Combustion characteristics are different between single coal and blended coal. Therefore, finding an optimal coal blending ratio according to coal rank effects on tar-soot yields.

• 한국신재생에너지학회:학술대회논문집
• /
• 2008.05a
• /
• pp.247-250
• /
• 2008

Biomass gasification is a promising technology for producing a fuel gas which is useful for power generation systems. In biomass gasification processes, tar formation often causes some problems such as pipeline plugging. Thus, proper tar treatment is necessary. So far, nickel (Ni)-based catalysts have been intensively studied for the catalytic tar removal. However, the deactivation of Ni-based catalysts takes place because of coke deposition and sintering of Ni metal particles. To overcome these problems, we have been using ruthenium (Ru)-based catalyst for tar removal. It is reported by Okada et al., that a Ru/$Al_2O_3$ catalyst is very effective for preventing the carbon deposition during the steam reforming of hydrocarbons. Also, this catalyst is more active than the Ni-based catalyst at a low steam to carbon ratio (S/C). Benzene was used for the tar model compound because it is the main constituent of biomass tar and also because it represents a stable aromatic structure apparent in tar formed in biomass gasification processes. The steam reforming process transforms hydrocarbons into gaseous mixtures constituted of carbon dioxide ($CO_2$), carbon monoxide (CO), methane ($CH_4$) and hydrogen ($H_2$).