• Korean Chemical Engineering Research
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• v.60 no.1
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• pp.169-174
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• 2022

In this study, a tandem water and hexane washing process was developed to improve the recovery efficiency of paclitaxel derived from Taxus chinensis. The polar impurities contained in the sample were effectively removed by washing with water at a sample/water ratio of 1:40 (w/v) for 10 min. In addition, the non-polar impurities were effectively removed by washing with hexane at a sample/hexane ratio of 1:160 (w/v) for 20 min. A high purity of paclitaxel (>30.0%) was obtained in a short operating time (~30 min) by sequential washing with water and hexane.

• Journal of the Korea Convergence Society
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• v.13 no.2
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• pp.85-91
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• 2022

As emphasized in the COVID-19 quarantine guidelines, hand washing is the most important prevention rule in tandem with distancing and mask. This study aimed to confirm relevant factors that affect practice of hand washing to find out approach for improvement of hand washing practice rate after COVID-19. Using the 2020 Community Health Survey data. As methods of research, this study searched for relevance by carrying out univariate logistic regression analysis, and also conducted multivariate logistic regression analysis using significant variables. Analysis results show that hand washing practice rate was high in females, well-educated, low age, cities, office job, the more people wear a face mask indoors, the higher the cycle of ventilation, the higher the cycle of disinfection and the more people maintain healthy distance. This study understood factors related to the rate of hand washing practice and results can be used as basic data for COVID-19 quarantine guidelines.

• Journal of Animal Environmental Science
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• v.14 no.3
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• pp.149-158
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• 2008

The purpose of this study was to determine the effect of milking system type on milking center effluent production through the four seasons. Four different types of milking systems (Bucket, Pipeline, Tandem and Herringbone) were estimated, in duplicate, through the different seasons. The following conclusions can be drawn from this study. 1. The quantity of wastewater produced from Tandem and Herringbone milking systems were significantly larger than Bucket milking system (p<0.05). 2. The main wastewater production was from the washing of milking apparatus. Tandem and Herringbone milking systems produced 398.8 and $407.7{\ell}$/day of wastewater, respectively, for apparatus washing. These values were significantly higher than the other milking systems during the summer (p<0.05). 3. The average wastewater production from the various milking systems was $15.4{\ell}$/head/day. The quantity of wastewater production during summer ($16.4{\ell}$/head/day) season was higher than of the other seasons. 4. The highest level of $BOD_5$ ($906.4mg/\ell$) was produced from the washing of the parlor floor and the lowest level of $BOD_5$ ($212.4mg/\ell$) was produced from the washing of the udders of the cows. 5. The pH of dairy wastewater was in the range of $7.3{\sim}8.2$ and the average levels of $BOD_5$, COD, SS, T-N, and T-P were 731.2, 479.0, 751.6, 79.1, $14.7mg/\ell$, respectively. Following conclusions can be drawn from this experiment. The quantities of wastewater production from Bucket, Pipeline, Tandem and Herringbone milking system were 143.9, 487.9, 914.0, and $856.7{\ell}$, respectively. The average wastewater produced from the milking systems was $15.4{\ell}$/head per day. In order to effectively manage on the wastewater from milking systems, dairy farms need to consider the milking system type and farm size when determining the optimum wastewater treatment system.

• Journal of Fashion Business
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• v.23 no.4
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• pp.100-111
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• 2019

In this study, the dyeing properties and functionalities of Crataegi fructus extract were investigated for the purpose of application to new natural dye resources. The effects of dyeing conditions(concentration of dye, dye bath temperature, dyeing duration and dye bath pH) and mordanting on dye uptake and color changes were also examined. The study also estimated the colorfastness, antibacterial properties, and ultraviolet protection properties of the dyed and mordanted silk fabrics. The dye uptake increased in tandem with the dye concentration and dye bath temperature. The highest K/S values were obtained at the following conditions: a dye concentration of 100%(v/v), a dyeing period of 60 minutes, a dye bath temperature of $90^{\circ}C$, and a dyeing pH of 3. The colorfastness of the material when dry cleaned or rubbed ranged from good to excellent and fastness to light was rated at grade 2-3. The change in the color grade when exposed to washing was not good; however, the stain of washing fastness was good at a range of 4-5. In regards to the functional property aspects, the dyed and mordanted fabrics exhibited excellent results with a 99.9% reduction rate, and excellent ultraviolet protection factors.

• Journal of Animal Environmental Science
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• v.7 no.2
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• pp.77-82
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• 2001

This research was carried out to investigate the daily amount and characteristics of wastewater produced from bucket milkers, pipeline, tandem ad herringbone milking system for washing operations after milking included 28 dairy farms. The average amount of wastewater produced from milking system was 9.8l/head/day. The amount of wastewater varied from a low of 8.2 litters/head/day(pipeline milking system) to 13.4 litter/head/day(herringbone milking system). The moisture content, Biochemical Oxygen Demand($BOD_5$), Chemical Oxygen Demand($COD_{Mn}$), Suspended Solids(SS), Total Nitrogen(T-N) and Total Phosphorus(T-P) concentration of wastewater were 99.9%, 394mg/l, 417.3mg/l, 1,201.3mg/l, 3.78mg/l, 0.51mg/l.

• YAKHAK HOEJI
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• v.52 no.5
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• pp.407-411
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• 2008

Phosphorylation plays the most important role in cell signaling mechanism. Various methods to identify the phosphorylation sites of proteins using tandem mass spectrometry (MS/MS) have been reported recently. Furthermore, the enrichment strategy such as Titanium dioxide ($TiO_2$) method should be combined with MS/MS analysis to effectively identify phosphorylation sites. It is necessary to optimize phosphopeptide-enrichment strategy, $TiO_2$ method in this study, due to the low amount of phosphorylated form followed by analyzing them by MS/MS. To evaluate the several conditions to enrich phosphopeptides using $TiO_2$ method, we used ${\apha}$-casein as a standard phosphoprotein and analyzed a representative phosphopeptide (VPQLEIVPNpSAEER) peak of MS spectrum. Batch is better than column method for binding and 300 g/l DHB in loading buffer is better than lower concentration of DHB. 3% TFA and pH 10.5 shows high efficiency of phosphopeptide-enrichment for washing and elution steps, respectively. Finally we identified various efficient conditions of phosphopeptide-enrichment method using $TiO_2$. This optimized method would assist in reliable identifying thousands of phosphorylation sites existed in low abundance from various complex proteins.

• Journal of Applied Biological Chemistry
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• v.65 no.3
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• pp.173-181
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• 2022

This study was carried out to investigate the residual characteristics of flubendiamide in kale to establish pre-harvest residue limits (PHRL) and the removal efficiency according to the washing solvent and method. Field tests were conducted at two different greenhouses, field 1 (Anseong-si, Gyeonggi-do) and field 2 (Incheon-si, Gyeonggi-do). According to the safe use guidelines kale was sprayed with flubendiamide twice every 10 days and harvested 0 (after 2 h), 1, 2, 3, 5, 7 and 10 days after the final application. The biological half-live of flubendiamide in kale was calculated based on dissipation curves of the pesticide in samples analyzed by liquid chromatography coupled with tandem mass spectrometry. In the analysis, method limits of quantitation (MLOQ) were 0.01 mg/kg, and recoveries performed with two different fortification levels of 10 MLOQ and maximum residue limit (0.7 mg/kg) were 104.2±3.6 and 101.9±10.2%, respectively. The dissipation rate constant of flubendiamide in kales were 0.2437 at field 1 and 0.1981 at field 2. PHRL calculation equations obtained using the dissipation constants estimated as follows: if the residual concentration of flubendiamide in kale on 10 days before harvest is less than 8.0 mg/kg, the residual concentration on the harvest would be under MRL. The removal of flubendiamide from kale was the greatest when it was washed with vinegar (39.8%), followed by baking soda (31.7%), calcium powder (30.2%), neutral detergent (27.2%), and tap water (15.9%). The results of this study would be useful for both farmers and consumers to produce or consume safe agricultural products.

• Analytical Science and Technology
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• v.12 no.3
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• pp.260-264
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• 1999

A simple and rapid procedure, called FoLT-PCR(Formamide Low Temperature-Polymerase Chain Reaction) was applied to amplifying DNA directly from various forensic biological evidences including human blood, saliva, hair root, or semen without any DNA preparative steps. We added washing step with non-ionic detergent, 1% Triton X-100, and used Taq DNA polymerase instead of Tth DNA polymerase to amplify 3 STR loci and gender allele simultaneouly. Optimal concentration of formamide and annealing temperature were determined empirically to 8%(v/v), and $48^{\circ}C$ respectively. We also compared this method with standard PCR.