• BMB Reports
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• v.37 no.1
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• pp.59-74
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• 2004

The study of whole patterns of changes in protein expression and their modifications, or proteomics, presents both technological advances as well as formidable challenges to biological researchers. Nutrition research and the food sciences in general will be strongly influenced by the new knowledge generated by the proteomics approach. This review examines the different aspects of proteomics technologies, while emphasizing the value of consideration of "traditional" aspects of protein separation. These include the choice of the cell, the subcellular fraction, and the isolation and purification of the relevant protein fraction (if known) by protein chromatographic procedures. Qualitative and quantitative analyses of proteins and their peptides formed by proteolytic hydrolysis have been substantially enhanced by the development of mass spectrometry technologies in combination with nanoscale fluidics analysis. These are described, as are the pros and cons of each method in current use.

• Journal of the Korean Electrochemical Society
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• v.20 no.1
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• pp.18-25
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• 2017

In this review, I have summarized the solar water splitting research based on the organic metal halide perovskite material, which has recently been spotlighted worldwide. Significantly, to date, recent reports have been categorized as photovoltaic-electrolyzer configuration and integrated photoelectrolysis. Research in this field is still in its early stages, and it is necessary to develop an effective protection film and manufacture a high-voltage tandem cell in the future.

• Journal of the Korea Society for Simulation
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• v.13 no.3
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• pp.1-10
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• 2004

The cell loss probability required in the ATM network is in the range of 10$^{-9}$ ∼10$^{-12}$ . If Monte Carlo simulation is used to analyze the performance of the ATM node, an enormous amount of computer time is required. To obtain large speed-up factors, importance sampling may be used. Since the Markov-modulated processes have been used to model various high-speed network traffic sources, we consider discrete time single server queueing systems with Markov-modulated arrival processes which can be used to model an ATM node. We apply importance sampling based on the Large Deviation Theory for the performance evaluation of, MMBP/D/1/K, ∑MMBP/D/1/K, and two stage tandem queueing networks with Markov-modulated arrival processes and deterministic service times. The simulation results show that the buffer overflow probabilities obtained by the importance sampling are very close to those obtained by the Monte Carlo simulation and the computer time can be reduced drastically.

• 한국정보디스플레이학회:학술대회논문집
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• 2007.08a
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• pp.977-979
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• 2007

We demonstrate a hybrid device with high ambien t-contrast-ratio (>133.8:1) under any ambient co nditions by vertically integrating a reflective LCD and a transparent OLED. The twisted nematic LC cell is placed beneath the OLED to improve dev ice transmittance by 53.8% due to the asymmet ric emission from both-sides of the transparent OLED.

• Parasites, Hosts and Diseases
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• v.62 no.3
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• pp.342-350
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• 2024

Although helminth parasites have different life cycles, their hosts share similar immune responses involving Th2 cell-type. Here, we extracted proteins from the larvae of Anisakis simplex complex and Trichinella spiralis to identify common and specific antigens (or allergens) associated with the Th2 immune response. We performed two-dimensional electrophoresis analysis and Matrix-assisted laser desorption ionization-time of flight/time of flight (MALDI-TOF/TOF) experiments. We found 13 potentially immunogenic proteins, which included 5 spots specific to T. spiralis and 8 common to T. spiralis and A. simplex, by tandem mass spectrometry. These molecules were identified structurally as actin, tropomyosin, col cuticle N domain-containing protein, and heat shock proteins. We also identified molecules related to parasite-host immune modulation and interactions. Our results may contribute to reveal potential roles of immunological proteins in parasite-derived immune modulation.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.6
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• pp.600-613
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• 2018

The purpose of this research was to introduce a network analysis method for analyzing the recent trend of the flexible solar cell using a scholarly database. Based on the five years from 2013 to 2017, we used centrality analysis of research papers via measurement of degree centrality, closeness centrality, and betweenness centrality. The results of network analysis show that cell has a centrality value above 0.8, which means that cell is connected with 80% of the total keywords, so it is recognized as the center of flexible solar cell research. The analysis results also indicate that perovskite and copper indium gallium diselenide (CuInGaSe2, or CIGS) are the center of the subgroup for cell. We recognize that the result refers to recent new technology called the CIGS/perovskite tandem solar cell. We hope that the network analysis method will be the appropriate and precise tool for technology and research planning via elaboration and optimization.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.11
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• pp.5409-5413
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• 2012

At present, multiple myeloma (MM) remains an incurable disease and cologenic cells may be responsible for disease relapse. It has been proposed that CD20+/CD138- NCI-H929 cells could be hallmarks of MM clonogenic cells. Here, the immunology phenotype of NCI-H929 cells is described. Only a small population of CD20+/CD138- cells (<1%) was found in the NCI-H929 cell line, but CD20+/CD138- cells were not detected. We found that CD20+/CD138+ cells were able to exhibit cologenic capacity by colony formation assay and continuous passage culture. Proteins were analyzed by 1D-SDS-PAGE and TMT based quantitative differential liquid chromatography tandem mass spectrometry (LC-MS/MS). 1,082 non-redundant proteins were identified, 658 of which were differentially expressed with at least a 1.5-fold difference. 205 proteins in CD20+ cells were expressed at higher levels and 453 proteins were at lower levels compared with CD20- cells. Most proteins had catalytic and binding activity and mainly participated in metabolic processes, cell communication and molecular transport. These results proved that there are different biological features and protein expression profile between CD20+ and CD20- cells in the NCI-H929 cell line.

• Nuclear Engineering and Technology
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• v.23 no.4
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• pp.420-425
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• 1991

The Rb charge exchange cell is constructed as the He- ion source of the SNU 1.5- MV Tandem Van do Graaff accelerator. The characteristic experiment is carried out in order to determine the optimum operational conditions of the cell. The $He^{+}$ ion beam with the energy of 1~10 keV, extracted from the duoplasmatron ion source, is passed through the Rb vapor to become He- ions by the two step charge exchange reaction, i.e., $He^{+}\;+\;Rb\;{\rightarrow}\;He^{\circ\ast}\;+\;Rb^{+}\;and\;He^{\circ\ast}\;+\;Rb\;{\rightarrow}\;He^{-}\;+\;Rb^{+}$. From the experimental results, it is found that the maximum fractional yield of $He^{-}$ ions is produced at He+ ion energy of 7 keV. The optimum temperatures of the oven and the canal are determined to be $370\;^{\circ}C{\;}and{\;}95^{\circ}C$ respectively. Under the optimum operational condition the maximum fractional yield of $He^{-}$ ions is $2.42\pm0.02%$ This charge exchange cell is proved to be an effective system for the production of He- ions.

• Journal of Animal Reproduction and Biotechnology
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• v.38 no.4
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• pp.291-299
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• 2023

Background: Leydig cells, crucial for testosterone production, express ion channels like ANO1 that influence hormone secretion. This study investigates the expression and role of the Tandem of P domains in a weak inward rectifying K⁺ channel-related Acid-Sensitive K⁺-1 (TASK-1) channel in these cells, exploring its impact on testicular function and steroidogenesis. Methods: TASK-1 expression in Leydig cells was confirmed using immunostaining, while RT-PCR and Western Blot (WB) validated its expression in the TM3 Leydig cell line. The effect of a TASK-1 channel blocker on cell viability was assessed through live/dead staining and MTT assays. Additionally, the blocker's effect on testosterone secretion was evaluated by measuring testosterone levels. Results: Immunohistochemical analysis revealed a predominant presence of TASK-1, along with c-Kit and ANO-1, in Leydig cells adjacent to seminiferous tubules and also in Sertoli and spermatogenic cells. Expression levels of TASK-1 mRNA and protein were significantly higher in TM3 Leydig cells compared to TM4 Sertoli cells. In addition, blocking TASK-1 in TM3 cells with ML365 induced cell death but did not affect LH-induced testosterone secretion. Conclusions: These findings suggest that TASK-1 in Leydig cells is crucial for their viability and proliferation, highlighting its potential importance in testicular physiology.

• Journal of Chest Surgery
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• v.33 no.1
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• pp.60-67
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• 2000

Background: Microsatellites are short-tandem repeated uncleotide sequences present throughout the human genome. Alterations of microsatellites have been termed microsatellite instability(MI). It has been generally known that microsatellite instability detected in hereditary non-polyposis colorectal cancer (HNPCC) reflects genetic instability that is caused by impairments of DNA mismatch repair system regarding as a novel tumorigenic mechanism. A number of studies reported that MI occurred at varying frequencies in non-small cell lung carcinoma (NSCLC). However It has been unproven whether MI could be a useful market of genetic instability and have a clinical significance in NSCLC. Material and Method : We have examined whether MI can be observed in thirty NCSLC using polymerase chain reaction whether such alterations are associated with other molecular changes such as p53, K-ras and c-myc oncoproteins expression detected by immunohistochemical stain,. Result: MI(+) was observed in 16.6%(5/30) and MI(-) was 83.3% (25/30) Average age was 50$\pm$7.5 year-old in MI(+) group and 57$\pm$6.6 year-old in MI(-) group. Two year survival rate in MI(=) group (20% 1/5) was worse than MI(-) group (64% 16/25) with a statistic difference. (P=0.04) The positive rate of K-ras oncoprotein expression and simultaneous expression of 2 or 3 oncoproteins expression were higher in MI(+) group than MI(-) group with a statistic difference(P=0.05, P=0.01) Conclusion: From, these results the authors can conclude that MI is found in some NSCLC and it may be a novel tumorigenic mechanism in some NSCLC. We also conclude that MI could be used as another poor prognostic factor in NSCLS.