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Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10

  • Yong-Min Kim;Sung-Woo Park;Mi-Jin Lee;Da-Yeon Jeon;Su-Jin Sa;Yong-Dae Jeong;Ha-Seung Seong;Jung-Woo Choi;Shinichi, Hochi;Eun-Seok Cho;Hak-Jae Chung
    • Journal of Animal Science and Technology
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    • v.65 no.2
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    • pp.401-411
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    • 2023
  • Many studies have been conducted to improve technology for semen cryopreservation in pigs. However, computer-assisted analysis of sperm motility and morphology is insufficient to predict the molecular function of frozen-thawed semen. More accurate expression patterns of boar sperm proteins may be derived using the isobaric tags for relative and absolute quantification (iTRAQ) technique. In this study, the iTRAQ-labeling system was coupled with liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify differentially expressed CM10-fractionated proteins between fresh and frozen-thawed boar semen. A total of 76 protein types were identified to be differentially expressed, among which 9 and 67 proteins showed higher and lower expression in frozen-thawed than in fresh sperm samples, respectively. The classified functions of these proteins included oxidative phosphorylation, mitochondrial inner membrane and matrix, and pyruvate metabolic processes, which are involved in adenosine triphosphate (ATP) synthesis; and sperm flagellum and motile cilium, which are involved in sperm tail structure. These results suggest a possible network of biomarkers associated with survival after the cryopreservation of Duroc boar semen.

HMM Based Part of Speech Tagging for Hadith Isnad

  • Abdelkarim Abdelkader
    • International Journal of Computer Science & Network Security
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    • v.23 no.3
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    • pp.151-160
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    • 2023
  • The Hadith is the second source of Islamic jurisprudence after Qur'an. Both sources are indispensable for muslims to practice Islam. All Ahadith are collected and are written. But most books of Hadith contain Ahadith that can be weak or rejected. So, quite a long time, scholars of Hadith have defined laws, rules and principles of Hadith to know the correct Hadith (Sahih) from the fair (Hassen) and weak (Dhaif). Unfortunately, the application of these rules, laws and principles is done manually by the specialists or students until now. The work presented in this paper is part of the automatic treatment of Hadith, and more specifically, it aims to automatically process the chain of narrators (Hadith Isnad) to find its different components and affect for each component its own tag using a statistical method: the Hidden Markov Models (HMM). This method is a power abstraction for times series data and a robust tool for representing probability distributions over sequences of observations. In this paper, we describe an important tool in the Hadith isnad processing: A chunker with HMM. The role of this tool is to decompose the chain of narrators (Isnad) and determine the tag of each part of Isnad (POI). First, we have compiled a tagset containing 13 tags. Then, we have used these tags to manually conceive a corpus of 100 chains of narrators from "Sahih Alboukhari" and we have extracted a lexicon from this corpus. This lexicon is a set of XML documents based on HPSG features and it contains the information of 134 narrators. After that, we have designed and implemented an analyzer based on HMM that permit to assign for each part of Isnad its proper tag and for each narrator its features. The system was tested on 2661 not duplicated Isnad from "Sahih Alboukhari". The obtained result achieved F-scores of 93%.

Development of Daily Life Monitori ng System using RFID (RFID를 이용한 일상생활 모니터링 시스템 개발)

  • Jung, Kyung-Kwon;Park, Hyun-Sik;Choi, Woo-Seung
    • Journal of the Korea Society of Computer and Information
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    • v.14 no.7
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    • pp.49-56
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    • 2009
  • In this paper, we present a daily activity monitoring system by using a wireless sensor network. The proposed system is installed in glove for activity monitoring. The RFID reader, to send data by using sensor network platform and RFID tag are small size, the shape of quadrangle, and operate in the frequency of 13.56 MHz. The sensor node can read RFID tags on the various objects used in daily living such as furniture, medicines, and kitchenwares. The sensor node reads the data of RFID tags, it transmits wireless packets to the sink node. The sink node sends the received packet immediately to a server system. The data from each RFID system is collected into a database, and then the data are processed to visualize the measurement of daily living activities of users. We provide a web-based monitoring system, and can see the number of RFID tag readings per day as bar charts. The result of experiments demonstrates that the way we propose can help to check the situation of life for people who live alone.

A RFID Tag Indexing Scheme Using Spatial Index (공간색인을 이용한 RFID 태그관리 기법)

  • Joo, Heon-Sik
    • Journal of the Korea Society of Computer and Information
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    • v.14 no.7
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    • pp.89-95
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    • 2009
  • This paper proposes a tag indexing scheme for RFID tag using spatial index. The tag being used for the inventory management and the tag's location is determined by the position of readers. Therefore, the reader recognizes the tag, which is attached products and thereby their positions can be traced down. In this paper, we propose hTag-tree( Hybrid Tag index) which manages RFID tag attached products. hTag-tree is a new index, which is based on tag's attributes with fast searching, and this tag index manages RFID tags using reader's location. This tag index accesses rapidly to tags for insertion, deletion and updating in dynamic environment. This can minimize the number of node accesses in tag searching comparing to previous techniques. Also, by the extension of MER in present tag index, it is helpful to stop the lowering of capacity which can be caused by parent node approach. The proposed index experiment deals with the comparison of tag index. Fixed Interval R-tree, and present spatial index, R-tree comparison. As a result, the amount of searching time is significantly shortened through hTag-tree node access in data search. This shows that the use of proposed index improves the capacity of effective management of a large amount of RFID tag.

AprilTag and Stereo Visual Inertial Odometry (A-SVIO) based Mobile Assets Localization at Indoor Construction Sites

  • Khalid, Rabia;Khan, Muhammad;Anjum, Sharjeel;Park, Junsung;Lee, Doyeop;Park, Chansik
    • International conference on construction engineering and project management
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    • 2022.06a
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    • pp.344-352
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    • 2022
  • Accurate indoor localization of construction workers and mobile assets is essential in safety management. Existing positioning methods based on GPS, wireless, vision, or sensor based RTLS are erroneous or expensive in large-scale indoor environments. Tightly coupled sensor fusion mitigates these limitations. This research paper proposes a state-of-the-art positioning methodology, addressing the existing limitations, by integrating Stereo Visual Inertial Odometry (SVIO) with fiducial landmarks called AprilTags. SVIO determines the relative position of the moving assets or workers from the initial starting point. This relative position is transformed to an absolute position when AprilTag placed at various entry points is decoded. The proposed solution is tested on the NVIDIA ISAAC SIM virtual environment, where the trajectory of the indoor moving forklift is estimated. The results show accurate localization of the moving asset within any indoor or underground environment. The system can be utilized in various use cases to increase productivity and improve safety at construction sites, contributing towards 1) indoor monitoring of man machinery coactivity for collision avoidance and 2) precise real-time knowledge of who is doing what and where.

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Sentiment Analysis of Korean Reviews Using CNN: Focusing on Morpheme Embedding (CNN을 적용한 한국어 상품평 감성분석: 형태소 임베딩을 중심으로)

  • Park, Hyun-jung;Song, Min-chae;Shin, Kyung-shik
    • Journal of Intelligence and Information Systems
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    • v.24 no.2
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    • pp.59-83
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    • 2018
  • With the increasing importance of sentiment analysis to grasp the needs of customers and the public, various types of deep learning models have been actively applied to English texts. In the sentiment analysis of English texts by deep learning, natural language sentences included in training and test datasets are usually converted into sequences of word vectors before being entered into the deep learning models. In this case, word vectors generally refer to vector representations of words obtained through splitting a sentence by space characters. There are several ways to derive word vectors, one of which is Word2Vec used for producing the 300 dimensional Google word vectors from about 100 billion words of Google News data. They have been widely used in the studies of sentiment analysis of reviews from various fields such as restaurants, movies, laptops, cameras, etc. Unlike English, morpheme plays an essential role in sentiment analysis and sentence structure analysis in Korean, which is a typical agglutinative language with developed postpositions and endings. A morpheme can be defined as the smallest meaningful unit of a language, and a word consists of one or more morphemes. For example, for a word '예쁘고', the morphemes are '예쁘(= adjective)' and '고(=connective ending)'. Reflecting the significance of Korean morphemes, it seems reasonable to adopt the morphemes as a basic unit in Korean sentiment analysis. Therefore, in this study, we use 'morpheme vector' as an input to a deep learning model rather than 'word vector' which is mainly used in English text. The morpheme vector refers to a vector representation for the morpheme and can be derived by applying an existent word vector derivation mechanism to the sentences divided into constituent morphemes. By the way, here come some questions as follows. What is the desirable range of POS(Part-Of-Speech) tags when deriving morpheme vectors for improving the classification accuracy of a deep learning model? Is it proper to apply a typical word vector model which primarily relies on the form of words to Korean with a high homonym ratio? Will the text preprocessing such as correcting spelling or spacing errors affect the classification accuracy, especially when drawing morpheme vectors from Korean product reviews with a lot of grammatical mistakes and variations? We seek to find empirical answers to these fundamental issues, which may be encountered first when applying various deep learning models to Korean texts. As a starting point, we summarized these issues as three central research questions as follows. First, which is better effective, to use morpheme vectors from grammatically correct texts of other domain than the analysis target, or to use morpheme vectors from considerably ungrammatical texts of the same domain, as the initial input of a deep learning model? Second, what is an appropriate morpheme vector derivation method for Korean regarding the range of POS tags, homonym, text preprocessing, minimum frequency? Third, can we get a satisfactory level of classification accuracy when applying deep learning to Korean sentiment analysis? As an approach to these research questions, we generate various types of morpheme vectors reflecting the research questions and then compare the classification accuracy through a non-static CNN(Convolutional Neural Network) model taking in the morpheme vectors. As for training and test datasets, Naver Shopping's 17,260 cosmetics product reviews are used. To derive morpheme vectors, we use data from the same domain as the target one and data from other domain; Naver shopping's about 2 million cosmetics product reviews and 520,000 Naver News data arguably corresponding to Google's News data. The six primary sets of morpheme vectors constructed in this study differ in terms of the following three criteria. First, they come from two types of data source; Naver news of high grammatical correctness and Naver shopping's cosmetics product reviews of low grammatical correctness. Second, they are distinguished in the degree of data preprocessing, namely, only splitting sentences or up to additional spelling and spacing corrections after sentence separation. Third, they vary concerning the form of input fed into a word vector model; whether the morphemes themselves are entered into a word vector model or with their POS tags attached. The morpheme vectors further vary depending on the consideration range of POS tags, the minimum frequency of morphemes included, and the random initialization range. All morpheme vectors are derived through CBOW(Continuous Bag-Of-Words) model with the context window 5 and the vector dimension 300. It seems that utilizing the same domain text even with a lower degree of grammatical correctness, performing spelling and spacing corrections as well as sentence splitting, and incorporating morphemes of any POS tags including incomprehensible category lead to the better classification accuracy. The POS tag attachment, which is devised for the high proportion of homonyms in Korean, and the minimum frequency standard for the morpheme to be included seem not to have any definite influence on the classification accuracy.

Regiospecific Orientation of Single-chain Antibody and Atomic Force Microscope (AFM) Images

  • Kyusik Yun;Park, Seonhee;Hyeonbong Pyo;Kim, Seunghwan;Lee, Sooyeul
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.4 no.1
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    • pp.72-77
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    • 1999
  • An antibody containing a genetically engineered lipid group at the N-termunus and a hexahistidinyl tag at the C-terminus (Lpp-scF-His6) was immobilized in an oriented manner on the surface of liposome. Liposomes, consisting of antibody and phosphatidyl-choline, have been prepared and imaged by AFM. For AFM visualization, the resulting liposomes were bound on the surface of mica by two different mechanisms. The histidine tags present in the antibody molecules of the immonuliposome were anchored to the NiCl2 treated mica surface. Alternatively, the immunoliposomes were immunochemically bound on antigen-coated mica surface. Both approaches yielded liposomes which were clearly imaged without damage by AFM in ambient condition.

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A Hypertension Management Healthcare System in Mobile Environment (모바일 환경에서의 고혈압관리 헬스케어 시스템)

  • Lee, Mal-Rey;Kim, Eun-Gyung;Lee, Jae-Wan;Zang, Yu-Peng
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.14 no.3
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    • pp.552-558
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    • 2010
  • This is an example of ABSTRACT format. This study proposes a hypertension management system that implements a signal collision avoidance algorithm for RFID tags and enables accurate medical services for hypertension patients. The proposed system enables the mobile RFID reader to accurately recognize the RFID tag signals emitted from the patient by using OR logic. Moreover, the system adopts a multi-agent approach to provide and manage information on patient condition and automated medical service in a mobile environment.

Study on the development of the KORMARC format for authority data (전기통제용 KORMARC형식의 개발에 관한 연구)

  • 윤구호
    • Journal of the Korean Society for information Management
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    • v.11 no.1
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    • pp.3-16
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    • 1994
  • The KORMARC format for authority data defines the ales and conventions (tags, indrators, subfield codes and coded value) that idenbfy the data elements in KORMARC authority records. This document is intended for the use of personel involved in the creation and maintenance of authority records as well as those involved in the deslgn and maintenance of systems for the communication and praxsmg of authority records. The format is developed on the basis of USMARC f o m t for authority data. And for the compatibility, the KORMARC format for bibliographc data is taken into account.

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Randomized Hash Lock Access Control Protocol for Mass RFID Tags (대량의 RFID 태그에 적용할 수 있는 확장성 있는 랜덤 해쉬락 접근제어 프로토콜)

  • Oh, Kyung-Hee;Kim, Ho-Won
    • Proceedings of the Korean Information Science Society Conference
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    • 2005.11a
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    • pp.109-111
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    • 2005
  • RFID는 기존의 바코드나 자기 인식 장치의 단정을 보완하고 사용의 편리성 향상으로 물류관리, 재고관리 등의 분야에서 활용 가능성이 비약적으로 증가되고 있는 차세대 핵심기술로 주목 받고 있다. 그러나 RFID 시스템이 활성화되기 위해서는 프라이버시 문제에 대한 해결책이 선행되어야만 한다. 해쉬락 기법은 리더가 태그를 인식하는 권한을 제어하여 임의의 리더가 태그 정보를 읽지 못하게 함으로써 프라이버시를 보호하는 기법이다. 본 논문은 기존의 해쉬락 기법에 의한 RFID 접근제어 프로토콜을 분석하고 취약점을 보완하여 대량의 태그를 사용하는 환경에서 도청자가 태그를 추적하지 못하도록 하는 접근제어 프로토콜을 제안한다.

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