• Biotechnology and Bioprocess Engineering:BBE
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• 제4권1호
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• pp.72-77
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• 1999

An antibody containing a genetically engineered lipid group at the N-termunus and a hexahistidinyl tag at the C-terminus (Lpp-scF-His6) was immobilized in an oriented manner on the surface of liposome. Liposomes, consisting of antibody and phosphatidyl-choline, have been prepared and imaged by AFM. For AFM visualization, the resulting liposomes were bound on the surface of mica by two different mechanisms. The histidine tags present in the antibody molecules of the immonuliposome were anchored to the NiCl2 treated mica surface. Alternatively, the immunoliposomes were immunochemically bound on antigen-coated mica surface. Both approaches yielded liposomes which were clearly imaged without damage by AFM in ambient condition.

• 대한가정학회지
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• 제36권5호
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• pp.137-150
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• 1998

The purpose of this study was to examine the effect of sex role orientation on the amount of household work time by husbands and wives in Seoul and Taejon. The data were collected from 100 couples in Seoul and 142 in Tadjon. The Major finding were as follows: 1. Taejon did not differed from Seoul in sex role orientation of the couples. 2. Sex role orientation of wife was related to the amount of time that husband spent on meal preparation and cleanup, clothing care, and child care. 3. Total household work time by husbands in Taejon was affected by the sex role orientation of himself, but the couples in Seoul and the wife in Taejon was not.

• The Korean Journal of Ecology
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• 제16권2호
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• pp.199-208
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• 1993

To estimate the level of the air pollution in Taejon city, the contents of S, Pb and Cd of the leaves of the roadside trees were deterimined. And we analyzed the relationship between the contents of S, Pb and Cd in the leaves of the roadside trees and traffic volume. The ranges of S, Pb and Cd contents in all the samples were 1.82~4.34 mg/g, 1.95~$246.80{\mu}g/g$ and 2.00~$20.50{\mu}g/g$ respectively. Areas with the high contents of S, Pb and Cd in the leaves were the road from Taejon railroad station to the provincial government, West Taejon crossroad, Taehwa-dong, Kasuwon-dong and Yongmun crossroad.

• 한국결정학회지
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• 제9권2호
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• pp.92-95
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• 1998

The structure of bis(isocyanto-N)nickel (II) complex, [Ni(L)(NCO)2] (L: 2,5,9,12-tetramethyl-1,4,8,11-tetraazacyclotetradecane), is centrosymmetric and the central nickel has an axially elongated octahedral geometry with two nitrogen atoms of the isocyanate ligand.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.377.2-377.2
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• 2002

The MeOH extract of the twig of Marus alba L. (Moraceae) inhibited strong lipid peroxidation activity. Five antioxidative compounds were isolated through activity-guided fractionation. and identified as 6-geranylapigenin (1). 6-geranylnorartocarpetin(2). resveratrol (3). oxyresveratrol (4). quercetin (5) by physicochemical and spectrometric methods. In order to evaluate the antioxidant effect of these compounds. the lipid peroxidation inhibitory activity test were performed. Compounds 1-5 showed greater activity than tocopherol. (omitted)

• Biotechnology and Bioprocess Engineering:BBE
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• 제3권2호
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• pp.61-66
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• 1998

Glycolipids produced by Pseudomonas aeruginosa YPJ-80 were characterized by chromatographic and spectorscopic techniques as a mixture of two rhamnolipids. For recovery of glycolipids from the culture broth, various isolation methods including ultrafiltration, adsorption and solvent extraction were compared. Ultrafiltration showed the best results in terms of glycolipids recovery. Further purification for spectroscopic analysis was carried out by adsorption chromatography and preparative thin layer chromatography. From the spectroscopic analysis, such as IR spectroscopy. FAB-MS, 1H-NMR and 13C-NMR and hydrolysis analysis, the glycolipids were identified as L-${\alpha}$-rhamnopyranosyl-${\beta}$-hydroxydecanoly-${\beta}$-hydroxydecanoate and 2-O-${\alpha}$-L-rhamnopyranosyl-${\alpha}$-L-rhamnopyranosyl-${\beta}$-hydroxydecanoyl-${\beta}$-hydroxydecanoate. Monorhamnolipid and dirhamnolipid lowered the surface tension of water to 28.1 mN/M and 29.3 mN/m, respectively.

• Journal of Microbiology
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• 제37권1호
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• pp.35-40
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• 1999

Sporulation-specific glucoamylase (SGA) gene was isolated from genomic library of Saccharomyces diastaticus 5114-9A by using glucoamylase non-producing mutant of S. diastaticus as a recipient. When the glucoamylase activities of culture supernatant, periplasmic, and intracellular fraction of cells transformed with hybrid plasmid containing SGA gene were measured, the highest activity was detected in culture supernatant. SGA produced by transformant and extracellular glucoamylase produced by S. diastaticus 5114-9A differed in enzyme characteristics such as optimum temperature, thermostability, and resistance to SDS and urea. But the characteristics of SGA produced by sporulating yeast cells and vegetatively growing transformants were identical.

• KIEE International Transactions on Power Engineering
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• 제11A권4호
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• pp.33-38
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• 2001

The KEPS(KEPCO＇s Enhanced Power system Simulator) Real Time Digital Simulator(RTDS) is the largest real time power system simulator ever built. A power system which includes 320 (3-phase) buses and 90 generators has been modeled and run in real time. Since such large-scale systems were involved, it was not practical to validate them using non-real time electro-magnetic transient programs such as EMTDC™ or EMTP. Instead, the results of the real time electromagnetic transient simulation were validated by comparing to transient stability simulations run using PTI's PSS/E™ program. The comparison of results from the two programs is very good in almost all cases. However, as expected, some differences did exist and were investigated. The differences in the results were primarily traced to the fact that the electromagnetic transient solution algorithm provides more detail solutions and therefore greater accuracy than the transient stability algorithm. After finding very good comparison of results between RTDS Simulator and PSS/E, and after investigating the discrepancies found, KEPCO gained the necessary confidence to use the large-scale real time simulator to analyze and develop their power system.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권4호
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• pp.234-241
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• 2000

The Saccharomyces cerevisiae PMR1 gene encodes a Ca2+-ATPase localized in the Golgi. We have investigated the effects of PMR1 disruption in S. cerevisiae on the glycosylation and secretion of three heterologous glycoproteins, human ${\alpha}$1-antitrypsin (${\alpha}$1-AT), human antithrombin III (ATHIII), and Aspergillus niger glucose oxidase (GOD). The pmr1 null mutant strain secreted larger amounts of ATHIII and GOD proteins per a unit cell mass than the wild type strain. Despite a lower growth rate of the pmr1 mutant, two-fold higher level of human ATHIII was detected in the culture supernatant from the pmr1 mutant compared to that of the wild-type strain. The pmr1 mutant strain secreted ${\alpha}$1-AT and the GOD proteins mostly as core-glycosylated forms, in contrast to the hyperglycosylated proteins secreted in the wild-type strain. Furthermore, the core-glycosylated forms secreted in the pmr1 mutant migrated slightly faster on SDS-PAGE than those secreted in the mnn9 deletion mutant and the wild type strains. Analysis of the recombinant GOD with anti-${\alpha}$1,3-mannose antibody revealed that GOD secreted in the pmr1 mutant did not have terminal ${\alpha}$1,3-linked mannose unlike those secreted in the mnn9 mutant and the wild type strains. The present results indicate that the pmr1 mutant, with the super-secretion phenotype, is useful as a host system to produce recombinant glycoproteins lacking high-mannose outer chains.

• 대한영양사협회학술지
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• 제5권2호
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• pp.117-127
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• 1999

This study was carried out to provide the improvable way of foodservice management to dietitian in the elementary schools. For this purpose the serving pattern of food, menu planning, nutrition education, leftover management and difficulties in foodservice were investigated. The survey was conducted through questionnaires, which were collected from 249 dietitions randomly selected in Taejon and Chung Nam. Data were analyzed by SAS program. The main results of this study are as follows. The children in Taejon took foods in the class room(65.7%) and those in Chung Nam in the dinning hall(89.9%)(p<0.01). In menu planning the balance of nutrition(42.6%) was mainly considered and then food preference(19.7%), variety of menus(16.1%), food cost(16.1%), composition of food color, taste and texture(3.2%), skills of food making(1.6%) and the number of students(0.8%). They referred cooking magazines(47.7%), the previous menu(42.2%), children's opinion(7.2%) for planning menu. The insufficiency of implements and devices for food(24.1%) and lack of season's food(20.4%) were indicated as difficulties in their job. 34.5% of dietitians used a standard menu, 79.5% investigated food preference of children, 74.3% evaluated taste of foods before serving, and 80.7% regularly checked leftover. The major reason for leftover was careless of the teacher in charge in Taejon and bad taste of foods nutrition in Chung Nam(p<0.001). 98.5% of them answered that they had the responsibility for nutrition education in elementary school. They had a hard time in relationship with the staffs in school(40.6%) and employees for food(39.8%), and lack of market information(38.2%). To efficiency of dietitian's work can be enhanced by reflecting the results of children's food preference and leftover, by using standard cooking method and proper cooking utensil, and by performing nutrition education for children by themselves.