• BMB Reports
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• 제37권6호
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• pp.741-748
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• 2004

The hepatitis C virus is associated with the development of liver cirrhosis and hepatocellular carcinomas. Among the 10 polyproteins produced by the virus, no function has been clearly assigned to the non-structural 5A (NS5A) protein. This study was designed to identify the hepatocellular proteins that interact with NS5A of the HCV. Yeast two-hybrid experiments were performed with a human liver cDNA prey-library, using five different NS5A derivatives as baits, the full-length NS5A (NS5A-F, amino acid (aa) 1~447) and its four different derivatives, denoted as NS5A-A (aa 1~150), -B (aa 1~300), -C (aa 300~447) and D (aa 150~447). NS5A-F, NS5A-B and NS5A-C gave two, two and 10 candidate clones, respectively, including an AHNAK-related protein, the secreted frizzled-related protein 4 (SFRP4), the N-myc downstream regulated gene 1 (NDRG1), the cellular retinoic acid binding protein 1 (CRABP-1), ferritin heavy chain (FTH1), translokin, tumor-associated calcium signal transducer 2 (TACSTD2), phosphatidylinositol 4-kinase (PI4K) and $centaurin{\delta}$ 2 ($CENT{\delta}2$). However, NS5A-A produced no candidates and NS5A-D was not suitable as bait due to transcriptional activity. Based on an in vitro binding assay, CRABP-1, PI4K, $CENT{\delta}2$ and two unknown fusion proteins with maltose binding protein (MBP), were confirmed to interact with the glutathione S-transferase (GST)/NS5A fusion protein. Furthermore, the interactions of CRABP-1, PI4K and $CENT{\delta}2$ were not related to the PXXP motif (class II), as judged by a domain analysis. While their biological relevance is under investigation, the results contribute to a better understanding of the possible role of NS5A in hepatocellular signaling pathways.

• 한국산림과학회지
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• 제4권1호
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• pp.9-13
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• 1965

Powdery mildew of Ailanthus altissima Sw. was first reported by J.S. Park in Korea, 1961. According to his report, this disease was found on the leaves of this tree species collected in Jeongup, 1957, and in Taejon, 1958. He described morphological characters of this pathogen and described also that the causal fungus may be closely related with Phyllactinia corylea. This disease which appears dusty grayish yellow molds under surface of leaves was collected again by the author in Kwangju, 1962. The present works were undertaken to make clear the species of causal fungus by means of the morphology and pathogenecity. According to the following results, the pathogen was identified as Phyllactinia corylea (Pers.) Karst. Morphological characters of the fungus Mycelia persistent or more or less evanescent; matured conidia solitary, aseptate, short-clavate to clavate to clavate, hyaline or yellowish, granulate, $44.6-89.2{\times}9.3-24.2{\mu}$, average $64.8-17.8{\mu}$ in size; conidiophores elongate clavate or columnar, hyaline, 2-3 septate, $122.8-346.0{\times}3.7-7.4{\mu}$, average $208.8{\times}6.3{\mu}$; per-thecia usually scattered, rarely gregarious, dark brown to black, depressed globose to globose, $223.2-297.6{\mu}$, average $267.8{\mu}$ in diameter; appendages 12-19, usually 15 in number, hyaline, straight, needle-shaped, sharply pointed at the apex and bulbous at the base, aseptate, $93.0-310.0{\times}5.0-8.0{\mu}$, average $173.3{\times}6.4{\mu}$ in size; asci elongate ellipsoidal to broadly clavate, hyaline, more or less stalked, 8-13 in number, $68.5-76.6{\times}26.1-34.2{\mu}$, average $71.4{\times}29.0{\mu}$ in size; ascospores 2-4, usually 2 in number, hyaline or yellowish, aseptate, ellipsoidal or ovate, $27.7-34.2{\times}14.7-17.9{\mu}$, average $25.5{\times}13.9{\mu}$ in size. Pathogenicity of the fungi In order to make clear the species of the fungus and the pathogenic differences of Phyllactinia fungi which are collected around the contaminated area and seemed to be related to Ailanthus powdery mildew, some inoculation experiments were performed. 1. Cross inoculation to several tree species with their pathogen: Tested materials; Phyllactinia in question on Ailanthus altissima Sw. Ph. fraxini (DC.) Homma. On Alnus firma S. et Z. Ph. moricola (P. Henn.) Homma on morus alba L. According to the results of the experiments, the reactions were all negative with the exception of the original hosts of tested fungi. 2. Inoculation to Picrasma ailanthoides Planch. : The appearance of symptom on the leaves of P. ailanthoides is not distinct, but more or less mycelial growth. Therefore, under the optimal condition in glass chamber, it may be possible to success artificially.

• 재활간호학회지
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• 제1권1호
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• pp.111-123
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• 1998

The related factors of the quality of life (QOL) in stroke patients was identified empirically. The subjects were 254 stroke patients who were discharged and taken follow-up care at the outpatient department. In this model, the physical, psychological, and social status were assumed to affect the QOL. And the social support was assumed to moderate these effects. NIH stroke state, ADL, and IADL were used to measure the physical status. Using CES-D, the psychological status was measured. The social status was defined as the job change after stroke attack. The satisfaction with the care by primary caregivers, significant others, and health professionals was measured as the social support. To identify the effect of the physical, psychological, and social status on the QOL, multiple regression analysis was carried out. The psychological and social status were found to be the significant predictors of the QOL(R2=0.27, p=0.00). Next, to identify the moderating effect of the social support, the subjects were divided into two groups, that is, the low social support group and the high social support group. It is found that the predicting variance is different between these two groups. In the low social support group, the psychological, social, and physical status predicted as much as 42% of the QOL. On the contrary, the psychological status predicted only 8% of the QOL in the high social support group. So it is concluded that the social support moderates the effects of the physical, psychological, and social status on QOL. Finally, to identify the social support which moderates those effects, the social support was divided into three classes. Each social support class was divided into the low and high social support group again. In the every class of social support, the difference between two groups was also identified. So the model of the QOL is recommended for the framework of the care for the stroke patients. Also these results support the claim that the long-term facilities for stroke patients are necessary.

• Applied Microscopy
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• 제31권2호
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• pp.167-173
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• 2001

한국재래산양의 임신60, 90, 105, 120일령 태아 및 신생아의 뇌를 대상으로 면역조직화학적방법을 통하여 면역반응을 나타낸 방사아교세포를 투과전자현미경적 방법을 이용하여 그 미세구조를 연구한 결과 임신 60일령 태아의 방사아교면역반응세포는 소수의 사립체와 많은 당원질 및 과립형질내세망이 관찰되었으며 임신90일령 태아 이후에서도 소수의 사립체, 많은 당원질 그리고 소수의 형질내세망이 관찰되었으며 임신95일령 태아에서 골지체가 관찰되었다. 그리고 임신 120일령에서 혈관에 부착되어 종말발을 뻗고 있는 GFAP 면역반응세포를 관찰할 수 있었다.

• 한국식품과학회지
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• 제17권3호
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• pp.155-162
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• 1985

양봉밀과 토종봉밀의 일반성분, 당, 유리아미노산 조성, 저장중의 diastase활성도 및 HMF 변화는 다음과 같다. 일반 성분 중 조회분은 밤꿀이 0.46%로 다른 벌꿀에 비하여 많았고, 조단백질은 양봉밀이 평균 0.10%로서 토종봉밀에 비하여 약 3.2배가 많았다. 무기물은 밤꿀에 있어서 Ca이 2.07mg%, P이 6.81mg%, Fe이 2.70mg%로 가장 많았다. 저장 중 diastase활성도 변화에 있어서 $4^{\circ}C$ 또는 $20^{\circ}C$저장 온도에서의 단기간 저장으로는 그의 활성도가 거의 감소되지 않았으며, $70^{\circ}C$에서 30분간 가열처리로 효소의 활성도가 감소되었으나 이를 $20^{\circ}C$에 저장 하였을 때는 그렇지 않은 것과 거의 동일 하였다. 양봉밀의 평균 당조성은 fructose 40.20%, glucose 32.43%, sucrose 1.09%, fructose/glucose 비는 1.24였다. 또한 토종봉밀은 fructose 37.37%, gluctse 31.92%, sucrose 0.74%였으며 fructose/glucose비는 1.19였다. 유리아미노산의 조성은 총 16종을 확인하였는데 그 중 proline함량이 가장 많아 그 함량은 총 유리아미노산의 39.59-46.86%를 차지하는 14.02-14.40mg%였다. 그러고 밤꿀에서 Phe 이 3.28mg%로 9.26%를 차지하였으며 광양산의 토종봉밀에서 Asp가 2.76mg%로 8.98%를 차지하였다. 저장 중 HMF 함량변화는 $4^{\circ}C$ 저장에 서는 거의 없었으나, $20^{\circ}C$, 80일간의 저장에서 밤꿀이 0.470mg%, 아카시아꿀이 0.336mg%, 유채꿀이 0.164mg%, 토종봉밀인 광양산이 0.426mg%, 광주산이 0.259mg%, 운봉산이 0.117mg%로 증가되었다. 또한 $50^{\circ}C$에서 3주간 저장으로 유채꿀이 8.71mg%, 아카시아꿀이 7.79mg%, 밤꿀이 5.35mg%증가되었다.

• 한국식품과학회지
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• 제19권2호
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• pp.81-88
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• 1987

동결속도를 달리하여 제조한 2종의 동결건조 분말고추장 재수화 현탁액의 리올리지 특성을 원료고추장을 대조구로 온도 $25{\sim}60^{\circ}C$, 고형물 함량 $47{\sim}56^{\circ}C$, 전단속도 $0.1965{\sim}1.9650\;sec^{-1}$의 범위에서 Brookfield 단 원통 회전점도계로 측정하였다. 급속동결 분말고추장 및 완만동결 분말고추장의 재수화 현탁액과 원료고추장은 모두 항복치를 지닌 의가소성을 나타내고. 전단초기 20분까지 Tiu의 모델에 따라 2차 반응속도식으로 붕괴되는 thixotropic거동을 보여주였으며. 분말고추장 현탁액의 구조붕괴 속도가 원료고추장 보다 빨랐다. 평형구조변수는 전단속도에 크게 영향받지 않았으며 그 값은 세가지 시료 모두 비슷하였다. 급속동결분말고추장 및 완만동결 분말고추장의 재수화 현탁액과 원료고추장의 곁보기점도의 온도 의존성은 Arrhenius식과 일치하였으며. 활성화 에너지의 값은 가각 2.21, 2.18, $2.32\;Kcal/g{\cdot}mole $이었다. 세가지 시료의 점조도지수는 온도의 증가에 따라 감소하었으며, 고형물 함량의 증가에 따라 증가하였으나, 유동거동지수는 온두 및 고형물 함량에 거의 영향을 받지 않았다. 급속동결 분말고추장과 완만동결 분말고추장의 러올로지 성질은 큰 차이가 없었다.

• Applied Biological Chemistry
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• 제13권1호
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• pp.65-72
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• 1970

Bence Jones 단백질중(蛋白質中) ${\lambda}$형(型)의 N-말단(末端) 및 그 주변(周邊)의 아미노산배열(酸配列)을 결정(決定)하기 위하여 본(本) 실험(實輸)이 시도(試圖)되었던바 그 결과(結果)는 다음과 같다. 1) Bence Jones 단백질(蛋白質)을 Pronase와 Chymotrypsin으로 분해(分解)하여 얻은 Peptide중에서 Im Pr-M 및 Im Ch-M와 Ik Ch-M을 Dowex $50{\times}2$ column $1{\times}20$cm)와 Dowex $1{\times}2$ column $(0.9{\times}50{\;}cm)$을 사용(使用)하여 분리(分離)하였다. 2) ${\lambda}$형(型) Bence Jones단백질(蛋白質)의 N-말단(末端)은pyrroglutamic acid로 되어 있음을 alkali반응(反應)과 고압여지전기영동법(高壓濾紙電氣泳動法)으로 확인(確認)하였다. 3) 농염산(濃鹽酸)(12N) 반응(反應)($27^{\circ}C$, 15시간(時間))을 이용(利用)하며 Peptide중(中)의 Serine부(部)를 선택적(選擇的)으로 절단(切斷)할 수 있었다. 4) 이들 Peptide의 아미노배열순서(配列順序)는 Edman의 PTC법(法)과 소거법(消去法) 및 CarboBypeptidase A를 사용(使用)하여 결정(決定)하였다. 5) 분리(分離)한 Peptide의 아미노산배열순서(酸配列順序)는 다음과 같았다. $Im\;Ch-M\;PCA{\cdot}Ser{\cdot}Val{\cdot}Leu$ $Ik\;Ch-M\;PCA{\cdot}Ser{\cdot}Ala{\cdot}Leu1$

• Applied Biological Chemistry
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• 제13권1호
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• pp.59-64
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• 1970

Bence Jones 단백질(蛋白質)(As,Ik,In)를 DEAE-Sephadex A-50 Column으로 0.02M phosphate Buffer(pH8.)와 0.5M NaCl를 gradient로 사용(使用)해 정재(精製)하였다. 시료(試料) As(K-형(型))의 정재결과(精製結果) 주성분(主成分)인 F-1는 조단백질(粗蛋白質) 500mg로부터 350mg 얻었으며 다른 시료(試料) Im와 Ik($lambda$-형(型))에서도 각각 주성분(主成分)을 242mg(Im)와 146mg(Ik) 얻을 수 있었다. 정재(精製)한 시료(試料)는 그의 순도(純度)를 알기 위해 Poly acryl amide의 Disc electrophoresis로 확인한 후 K-형(型) Bence jones단백질(蛋白質)의 일종(一種)인 As와 $lambda$-형(型)의 일종(一種)인 IM를 6N-HCI로 $105^{\circ}C$에서 20시간(時間) 가수분해(加水分努)후 Amino 산(酸) 조정(組成)을 살펴보았다. DNP법(法)으로 시료(試料) K-형(型)(As, Ko, Ta)의 단백질(蛋白質)의 N-말단(末端)을 검출(檢出)해 Ta는 glutamic acid, Ko, As는 Aspartic acid임을 확인하였으며 Paper 상(上)에 나타난 DNP-Amino 산(酸)은 5% $NaHCO_3$ (pH8) 4ml에 추출(抽出)해 그 수량(收量)을 ${\varepsilon}=18.1{\times}10^3DNP$ $Asp\;{\varepsilon}=17.41{\times}10^(3){\;}DNP{\;}Glu$에 의(依)해 산출(算出)하였더니 Ko는 54.3%, As는 65%이였으며 Ta는 85%의 수량(收量)이였다. ${\lambda}$-형(型)의 Im,Ik의 시료(試料)도 DNP법(法)으로 N-말단(末端)을 검출(檢出)해 보았더니 검출(檢出)되지 아니하였다.

• 대한예방한의학회지
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• 제1권1호
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• pp.126-136
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• 1997

To procide basic data for improvement of oriental medical care system, the questionnaire interview was carried out with community peoples, over 20 years old, selected randomly in Seoul, Pusan, Taegu and Taejon err. from 10th Mar. to 20th Apr. 1996. The result were summarized as follows: 1. In the general characteristics of subjects, the highest portion of each part was 52.4% of male, 22.4% of thirties, 37.1% of college graduates, 19.1% of married, 30.6% of town dwellers, 63.3% of muddle social class, 26.6% of housewife, 19.3% of student, 16.6% officer and 11.6% of professional technician. 2. The portion of subjects agree to open oriental clinic in western medicine hospital was 60.0%, that of disagreement was 20.1%, Among 60.0% of agreement group, the high rate was showed in female, fifties, middle school graduates, unemployed, middle social level. 3. The rate of positive responses in system of a herb specialist was 64.7%, that of negative responses was 11.6%. Among positive responses, the groups of high rate were male. twenties, above college graduates, student, middle-high social level and city dwellers. 4. In the question whether a pack of herb medicine should be included in medical insurance or not, the rate of agreement was 74.3%, that of disagreement was 4.1%. Amount agreement subjects, the high rate was showed in male, young age, high educated, city dwellers, professional technician middle social class. 5. In the cost of oriental medical care, the portion was divided by 70.3% of expensiveness,25.6% of moderate, 4.1% of low price. among 70.3% of high price, the high rate was in female, forties, agrolivestock-fishery, and town dwellers. 6. In the question what the herb doctor should do for the improvement of oriental medicine, the highest rate was 54.2% in further study. The next was 23.0% in need of western medical and 9.1% in no complain in present situation. Among 54.2% of further study, the high rate was showed in male, forties, high school graduate, profession technician, high social level, christian, married and city dwellers. 7. About institution of oriental care, 86.8% of subjects answered to need of improvement, 8.0% of them replied no problem as present. Amount 86.8% of subjects, the high rate was showed in male, twenties, above college graduate, sales, singles, high social level and city dwellers. 8. About necessity of western medical care instrument in oriental medical hospital, the rate of sightly need was 47.5%, inevitably need was 37.7%, no need was 6.3%, the Positive subjects were showed high rate in male, officer, singer, the younger age, the higher educated, better social level and city dwellers. These results might be useful information for establishing of oriental medical care policies, which open oriental medical clinic in western medical hospital, equip western medical care instrument in oriental medical hospital, improve oriental medical care instrument carry out a herb specialist system, balance the resonable medical care cost and effort for further study & research on oriental medicine to satisfy increasing need of oriental medicine.

• Parasites, Hosts and Diseases
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• 제35권1호
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• pp.9-17
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• 1997

1993-1994년에 걸쳐 전국 10개 지역내 주택에서 수거한 집먼지내의 진드기류를 조사하였다. 집 먼지는 평소 가정주부가 사용하는 전기진공청소기내 횔터에 모아진 집먼지 중 10 g을 채취하여 wet sieving metrod 일부 보완하여 진드기류를 분리하였다 채집된 총 7,257개체의 진드기를 동정한 결과 4아목 18과 23속 23종을 확인하였는데 그 중 Rhyzoglyphus robini, Sancossania phwllophcgianus, Cheyletus trouessarti 및 Scheloribotes lotipes의 4종은 국내 미기록 종이었다 전국적으로 가장 널리 분포되어 있고 서식밀도가 높은 종은 nemntophago릴os Jnrinae(DF)로 전체의 65.3%였고 D. Pteronvssinus(DP)가 20.6% Twrophnsus putrescentiqe(TP)가 6.5%를 차지하였는데, 이들 3종이 함께 서식하는 가정은 62개 조사대상 중 24.6%였고. 2개종이 공존하는 가정은 48 1%. 한종만 서식하는 가정은 27.3%였다. DF가 우점 종인 가정은 59개 조사 가정 중 63.5% DP가 우점종인 가정은 29.6%였고 TP가 우점종인 가정은 6.9%였다. 지역적으로는 서울. 광주 인천 전주 정주 등지에서는 DF가 우세하였으나 영광 춘천. 부산 영도 등 대기습도가 비교적 높은(> 73% RH) 지역에서는 DP가 우세한 것으로 나타났다. 집먼지진드기의 서식밀도를 보면 집먼지 10g 중 1.000개체 이상인 가정이 2.9%. 500-999 개체의 가정이 4.3%. 100-499개체의 가정이 15.7%였고 99개체 이하의 밀도를 보인 가정이 70.0%를 차지하였으며 전혀 채집되지 않은 가정은 7.1%였다. 아파트와 단독주택. 건물면적 청소횟수. 가족수. 건축년수 등과 집먼지진드기 서식밀도간에는 상관관계를 보이지 않았다.